FastQCFastQC Report
Fri 17 Jun 2016
SRR1526030_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526030_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48954
Sequences flagged as poor quality0
Sequence length56
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG4060.8293500020427339No Hit
ACACCCGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT1430.2921109613106181No Hit
ACACCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG1320.2696408873636475No Hit
ACACCCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC1310.26759815336846837No Hit
ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1270.25942721738775176No Hit
ACACCCGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC1070.2185725374841688No Hit
ACACCCGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA890.18180332557094417No Hit
ACACCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC810.16546145360951098No Hit
ACACCCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC770.15729051762879437No Hit
ACACCCGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC750.15320504963843606No Hit
ACACCCGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC670.1368631776770029No Hit
ACACCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG670.1368631776770029No Hit
ACACCCGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT630.12869224169628632No Hit
ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC560.11439310373003227No Hit
ACACCCGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC550.11235036973485313No Hit
ACACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGT540.11030763573967398No Hit
ACACCCGGGAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGA500.10213669975895738No Hit
ACACCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG500.10213669975895738No Hit
ACACCCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT490.10009396576377826No Hit
ACACCCGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG490.10009396576377826No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCC251.9120784E-550.6233141
CGTCTCA203.8979645E-450.62330641
TTCAATC203.938242E-450.51817340
CTCGTCT251.9358016E-550.5181739
GTTCAAT251.9358016E-550.5181739
GCTCGTC251.9358016E-550.5181738
AGACCTT150.0080807550.46576737
CAGGACT309.772302E-750.36128235
CACGATT150.008214300550.2572347
CGCCCAA309.913128E-750.2572347
CCACAAC150.00824794450.20536413
ACAACTA150.00824794450.20536415
CTCCACG150.00824794450.20536444
GACTTAA150.00824794450.20536410
ATCCGCC354.986032E-850.20536444
CCCCAAC204.061062E-450.20536413
GACGAGA150.00824794450.20536413
GCCACAA150.00824794450.20536412
CGCCACA150.00824794450.20536442
AGGTCAT150.00831553650.10195549
Produced by FastQC (version 0.11.5)