##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526030_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 48954 Sequences flagged as poor quality 0 Sequence length 56 %GC 57 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.07157739919108 39.0 38.0 39.0 33.0 40.0 2 36.425603627895576 39.0 38.0 39.0 30.0 40.0 3 36.552314417616536 39.0 37.0 39.0 33.0 40.0 4 36.05496997181027 39.0 38.0 39.0 30.0 40.0 5 36.27217387751767 39.0 38.0 39.0 30.0 40.0 6 36.06003595211831 39.0 37.0 39.0 30.0 40.0 7 35.5170772561997 38.0 35.0 39.0 30.0 40.0 8 35.79985292315235 39.0 36.0 39.0 30.0 40.0 9 35.99519957511133 39.0 36.0 39.0 30.0 40.0 10 35.29531805368305 39.0 35.0 39.0 28.0 40.0 11 34.738039792458224 38.0 35.0 39.0 27.0 40.0 12 33.35643665481881 38.0 32.0 39.0 20.0 40.0 13 32.93136413776198 37.0 32.0 39.0 17.0 40.0 14 33.54516484863341 38.0 33.0 39.0 20.0 40.0 15 32.66350042897414 37.0 31.0 39.0 15.0 40.0 16 31.7126690362381 36.0 29.0 39.0 15.0 40.0 17 30.90184663153164 35.0 27.0 38.0 13.0 40.0 18 30.153777015157086 35.0 25.0 38.0 11.0 40.0 19 31.40819953425665 36.0 28.0 38.0 16.0 40.0 20 31.785247375086815 36.0 29.0 38.0 17.0 40.0 21 31.910323977611636 36.0 29.0 39.0 16.0 40.0 22 31.186522041099806 36.0 28.0 39.0 13.0 40.0 23 31.856375372798954 36.0 29.0 39.0 16.0 40.0 24 31.28573763124566 36.0 28.0 39.0 13.0 40.0 25 31.65800547452711 36.0 29.0 39.0 15.0 40.0 26 32.27415532949299 36.0 31.0 39.0 15.0 40.0 27 32.30373411774319 36.0 31.0 39.0 16.0 40.0 28 32.2035584426196 36.0 31.0 39.0 15.0 40.0 29 31.833598888752707 36.0 30.0 39.0 13.0 40.0 30 31.586571066715692 36.0 30.0 39.0 11.0 40.0 31 32.284042162029664 36.0 32.0 39.0 11.0 40.0 32 32.11032806307963 36.0 31.0 39.0 10.0 40.0 33 31.910323977611636 36.0 31.0 39.0 10.0 40.0 34 31.767434734648855 36.0 31.0 39.0 4.0 40.0 35 31.44000490256159 36.0 30.0 38.0 4.0 40.0 36 30.623217714589206 35.0 29.0 38.0 4.0 39.0 37 30.3546186215631 35.0 29.0 38.0 4.0 39.0 38 29.863177677002902 35.0 28.0 38.0 4.0 39.0 39 29.03946562078686 34.0 26.0 38.0 4.0 39.0 40 26.825713935531315 33.0 17.0 38.0 4.0 39.0 41 25.129264207214938 30.0 12.0 36.0 4.0 39.0 42 25.88789475834457 31.0 17.0 36.0 4.0 39.0 43 25.69747109531397 31.0 15.0 36.0 4.0 39.0 44 24.845589737304408 30.0 11.0 36.0 4.0 39.0 45 25.628426686276914 31.0 15.0 36.0 4.0 39.0 46 23.8163990685133 29.0 4.0 35.0 4.0 39.0 47 22.85551742452098 27.0 4.0 35.0 4.0 38.0 48 23.338828287780366 28.0 4.0 35.0 4.0 38.0 49 22.36223801936512 27.0 4.0 35.0 4.0 38.0 50 22.843832986068556 28.0 4.0 35.0 4.0 38.0 51 22.992503166237693 29.0 4.0 35.0 4.0 38.0 52 22.776402336887692 28.0 4.0 35.0 4.0 38.0 53 22.53774972423091 28.0 4.0 35.0 4.0 38.0 54 22.137108305756424 28.0 4.0 35.0 4.0 38.0 55 21.789986517955633 27.0 4.0 34.0 4.0 38.0 56 21.460227969113863 27.0 4.0 35.0 4.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 2.8378520360492843 1 2 3.7339973098805217 1 3 3.2845146643132708 1 4 4.244375500189221 1 5 4.005391703567248 1 6 4.191062398147196 1 7 3.741822996586386 1 8 3.8189961651841386 1 9 3.79700383780564 1 10 4.280453855721682 1 11 0.35857984466297665 1 12 -7.682132610345555 1 13 -18.2622265611135 1 14 -7.178481409275456 1 15 1.2701766071454053 1 16 -0.9450299408239893 1 17 -5.566100663139011 1 18 -4.553923896111069 1 19 1.539427802845271 1 20 0.12315327548638066 1 21 7.4892578734402235 1 22 8.475516099106802 1 23 7.572472848979352 1 24 8.2328372745446 1 25 7.78498209610461 1 26 7.801172973926782 1 27 7.728445626621557 1 28 7.9400726272615145 1 29 8.242481473228203 1 30 7.555600042715962 1 31 7.062844545647696 1 32 6.6140271444767365 1 33 6.845966283234976 1 34 5.355700062358888 1 35 7.257899232780609 1 36 4.635760045287864 1 37 4.5285837300355 1 38 4.566859468908579 1 39 3.65313932331798 1 40 -9.688125706705414 1 41 -0.2989989423749826 1 42 -1.112292760295741 1 43 -9.110754460559553 1 44 -13.428136875148502 1 45 -2.638022271970353 1 46 -4.745088034148985 1 47 -9.022514073209862 1 48 -4.344068372724713 1 49 -11.098592087062839 1 50 -2.9766700591885566 1 51 2.0906504277280895 1 52 2.0711846338491533 1 53 2.3567720510537953 1 54 2.0003369357983054 1 55 1.5352384173222369 1 56 4.723926807818721 2 1 3.1801039869968832 2 2 4.1933061170154105 2 3 3.795662936331105 2 4 4.680830349687554 2 5 4.534934624414518 2 6 4.7206053189944654 2 7 4.203361458124853 2 8 4.141181226499633 2 9 4.38451777314566 2 10 4.784355750927922 2 11 4.53695219694837 2 12 0.6517581365886684 2 13 3.246692056500766 2 14 5.812042559509383 2 15 5.929039483399585 2 16 6.4273223445717775 2 17 8.402126761610155 2 18 6.737603417155377 2 19 7.671534826256639 2 20 7.098627077047137 2 21 8.486470805435765 2 22 9.527355563989747 2 23 8.227991243628182 2 24 9.164275401634903 2 25 7.815639844153662 2 26 7.762711435465242 2 27 7.574042059174509 2 28 7.350886451118818 2 29 7.1064725546105905 2 30 7.077339173150744 2 31 6.241216897933096 2 32 6.527628036338498 2 33 6.123558256479125 2 34 6.3696354023811885 2 35 6.772391986403797 2 36 7.7912784399366934 2 37 7.734269348764592 2 38 8.159390126656628 2 39 8.150352255313518 2 40 6.541528696862034 2 41 11.507578538115542 2 42 9.140773014509165 2 43 8.887015885036877 2 44 -3.70628626199354 2 45 8.138454874072007 2 46 6.757699033855477 2 47 4.023193842063272 2 48 6.757938316238498 2 49 0.45491961862278174 2 50 7.5512006208560365 2 51 11.473036157939909 2 52 10.348219193492408 2 53 10.788767591744989 2 54 10.81917751550845 2 55 10.959987581201837 2 56 11.461942415399545 3 1 3.2237886973482617 3 2 4.331957589000218 3 3 3.3564416637765007 3 4 4.668959504483375 3 5 4.298467387947284 3 6 4.521175119564269 3 7 4.0751563299197215 3 8 4.043365462017199 3 9 4.0739764626043495 3 10 4.363652996891837 3 11 5.287189613852455 3 12 6.236278554442421 3 13 6.3706236804323915 3 14 6.270257184390676 3 15 0.5235314112248481 3 16 7.788196038778807 3 17 9.035080227896948 3 18 9.4294362756549 3 19 7.597935585990733 3 20 7.256271901358627 3 21 8.625597111228736 3 22 9.455655658956513 3 23 8.080792763096369 3 24 8.848510919203747 3 25 8.198830727344546 3 26 7.62833346775394 3 27 7.88410853590765 3 28 7.809101076000111 3 29 8.409416524221228 3 30 7.805734234879136 3 31 6.843306166689029 3 32 6.964000306044106 3 33 6.9801584464126485 3 34 6.908571774650891 3 35 7.741527788872929 3 36 8.255191070515995 3 37 8.558106005934583 3 38 9.430520231120067 3 39 9.307997079625007 3 40 10.759002581002587 3 41 12.97528983916018 3 42 11.8093390164085 3 43 12.289674954362614 3 44 11.963704241330298 3 45 12.116137685088155 3 46 13.822276431766209 3 47 13.089670575206672 3 48 13.4906068822404 3 49 13.85900318937302 3 50 13.58586348885224 3 51 12.997252682156432 3 52 12.286965632238847 3 53 11.952585255562653 3 54 12.356214552545485 3 55 11.673187961068884 3 56 11.576852196975995 4 1 2.9122227454314356 4 2 4.283387088675333 4 3 3.370899103672535 4 4 4.3778613888238525 4 5 3.628389415237727 4 6 4.432481162449257 4 7 3.917261593077619 4 8 4.004541550386271 4 9 4.284990107828527 4 10 4.6753813919535645 4 11 5.416169470902489 4 12 6.118019944955741 4 13 6.689337130724791 4 14 6.411907606742847 4 15 6.5636548680149716 4 16 8.705999807459769 4 17 9.285730000476548 4 18 9.855687087870628 4 19 7.837864111006979 4 20 8.096265403632831 4 21 8.001652991670582 4 22 7.977423040373015 4 23 8.157141041199033 4 24 8.849160691783347 4 25 7.846004216623296 4 26 7.717839640593446 4 27 7.147591316934296 4 28 6.968295358001413 4 29 7.36052113760654 4 30 7.472076015256004 4 31 5.901135926273174 4 32 5.980731949968728 4 33 6.216675665386003 4 34 6.034952541382534 4 35 6.346953389912564 4 36 6.509901921718072 4 37 6.784876636213987 4 38 6.721131017344884 4 39 7.319043213478164 4 40 1.8650779546218175 4 41 11.11823980667155 4 42 9.349949802633319 4 43 9.728109002445787 4 44 10.37907136283777 4 45 9.755189017121943 4 46 11.075850180953992 4 47 12.29613581237366 4 48 11.855779071973991 4 49 12.378333923616033 4 50 12.153764723420139 4 51 11.3681103819615 4 52 11.146127425611166 4 53 11.646055041137704 4 54 12.586721375157573 4 55 12.277801346384024 4 56 11.402225816209263 5 1 3.3466041534970543 5 2 4.361138284847584 5 3 3.689935330603504 5 4 4.459884628741833 5 5 4.366769456249351 5 6 4.604388202777351 5 7 4.0361952909586805 5 8 4.08024216556057 5 9 3.965270353898241 5 10 4.672134638706808 5 11 5.711111371107549 5 12 6.732510717341249 5 13 7.030075339884053 5 14 6.686001933468756 5 15 7.729239283599384 5 16 8.785630702880134 5 17 9.734294593777982 5 18 10.19198237153433 5 19 8.221151875873694 5 20 8.168890147310211 5 21 7.701755520720475 5 22 9.470246006880192 5 23 7.972246987883928 5 24 8.911361648721147 5 25 8.655300183814003 5 26 7.376930549684353 5 27 7.279170264302714 5 28 7.532205132437504 5 29 8.160418608556647 5 30 8.181235277046849 5 31 6.891566548282743 5 32 7.114874123584585 5 33 6.948733081653952 5 34 6.938233470979252 5 35 7.471526185537996 5 36 8.412157560815817 5 37 8.934569049064294 5 38 8.488560955827456 5 39 9.038797143758405 5 40 10.292111447444785 5 41 13.42582695636396 5 42 13.11413298786914 5 43 12.399503397524395 5 44 12.569604513897232 5 45 12.033726269343404 5 46 13.544578820735264 5 47 13.878671697541126 5 48 13.449080507380685 5 49 14.96257862628179 5 50 14.179738749394161 5 51 13.8693065542103 5 52 13.07965442492764 5 53 12.900156203133598 5 54 13.873290069621003 5 55 14.129817751032004 5 56 13.192212145669277 6 1 2.936514243407146 6 2 3.847152270861571 6 3 3.141816782484952 6 4 4.398779067636269 6 5 3.958011547491438 6 6 4.143682242071385 6 7 3.9084896632530572 6 8 3.615540200858611 6 9 3.871697260325149 6 10 4.4125608791330535 6 11 4.421567581563757 6 12 6.369706854537391 6 13 6.105666415475131 6 14 5.799222046688872 6 15 6.8136548680149716 6 16 8.735014652264084 6 17 8.838024197507586 6 18 10.083757263309224 6 19 8.427945082666902 6 20 6.944780923200984 6 21 8.19159901056397 6 22 8.950432487066674 6 23 7.945939961576904 6 24 8.40786514522464 6 25 8.277178305692122 6 26 7.390916563670366 6 27 7.458657443789896 6 28 7.607296707529073 6 29 8.18339563153367 6 30 8.34656994238151 6 31 6.189934846651045 6 32 6.668653677364141 6 33 5.8030454359663 6 34 6.177327710073495 6 35 7.1826483966602055 6 36 7.432304080962336 6 37 7.5932437077389565 6 38 7.620928588195088 6 39 8.688813793775058 6 40 4.413323568656907 6 41 12.456296486833491 6 42 11.448465322201471 6 43 11.06650306452406 6 44 12.088585532878255 6 45 11.484608720225857 6 46 13.680775956932397 6 47 14.66421948308891 6 48 13.257938316238494 6 49 13.493381157084322 6 50 12.884533954189372 6 51 13.421754106657854 6 52 13.271296116569332 6 53 13.916972719950117 6 54 13.447382643713578 6 55 13.818961940176198 6 56 13.192711646168775 7 1 3.134591166484064 7 2 3.882729193938488 7 3 3.6366885773567503 7 4 4.518650862508068 7 5 4.233652573132467 7 6 4.456823267712416 7 7 3.2751563299197244 7 8 3.6334889188073234 7 9 4.091569055196942 7 10 3.7253813919535617 7 11 5.439195786691961 7 12 6.23380941863995 7 13 6.676179235987949 7 14 6.267170764637587 7 15 6.701154868014974 7 16 8.387578754828183 7 17 9.262703684687075 7 18 10.01292392997589 7 19 8.426022005743825 7 20 7.213370666790727 7 21 8.334547728512682 7 22 8.774791461425647 7 23 8.332798935935877 7 24 8.901134375993877 7 25 8.249293690307507 7 26 7.230339640593449 7 27 7.079170264302711 7 28 6.864347989580359 7 29 7.127626400764434 7 30 7.002339173150741 7 31 5.739293821010019 7 32 5.572179318389786 7 33 6.722596718017588 7 34 6.437584120329905 7 35 5.489058653070458 7 36 7.43358613224439 7 37 7.2736924256876705 7 38 8.138236280502785 7 39 8.240095845057109 7 40 8.484156901990236 7 41 11.639950332987336 7 42 10.342055065791214 7 43 10.462977423498419 7 44 10.850123994416712 7 45 9.277557438174576 7 46 11.355455444111886 7 47 11.5263989702684 7 48 10.177489598289778 7 49 12.502676028879193 7 50 10.916264723420138 7 51 10.272715645119394 7 52 10.527706372979587 7 53 10.049344514821911 7 54 10.36501084884178 7 55 9.88306450427876 7 56 9.143673184630316 8 1 2.7881625950554962 8 2 3.9988006225099184 8 3 2.95097429164246 8 4 4.450793719650925 8 5 4.18365257313247 8 6 4.5264661248552684 8 7 3.8751563299197187 8 8 4.119203204521611 8 9 4.266569055196946 8 10 4.668238534810705 8 11 5.355267215263389 8 12 5.99095227578281 8 13 6.469036378845093 8 14 6.417170764637586 8 15 6.985083439443542 8 16 8.337578754828186 8 17 9.396632256115645 8 18 9.693281072833031 8 19 8.165307720029539 8 20 7.688370666790728 8 21 7.966690585655542 8 22 8.453362889997077 8 23 7.434584650221588 8 24 8.695777233136731 8 25 7.318936547450367 8 26 5.996411069164878 8 27 6.9791702643027165 8 28 6.003633703866072 8 29 6.1097692579072955 8 30 7.22019631600789 8 31 5.4357223924385885 8 32 4.838250746961215 8 33 5.270811003731868 8 34 5.559012691758472 8 35 5.3962015102133165 8 36 5.797871846530104 8 37 5.854049568544816 8 38 5.913236280502783 8 39 7.125810130771395 8 40 2.6895140448473818 8 41 10.616736047273054 8 42 9.449197922934076 8 43 10.339763137784129 8 44 10.235838280130999 8 45 9.002557438174573 8 46 11.467955444111883 8 47 11.379970398839827 8 48 10.425703884004065 8 49 12.279461743164905 8 50 10.725193294848712 8 51 10.574501359405108 8 52 11.1419920872653 8 53 10.883273086250483 8 54 11.00965370598464 8 55 11.525921647135903 8 56 10.874030327487457 9 1 3.200170876628995 9 2 4.139794411329795 9 3 3.4729204614147164 9 4 4.548723326276182 9 5 4.280754022407827 9 6 4.553381238726907 9 7 3.872257779195081 9 8 3.456677324604428 9 9 4.333235721863609 9 10 3.9181350151419707 9 11 4.981768250460078 9 12 5.607722462118211 9 13 6.317483583814035 9 14 6.198330184927439 9 15 7.168727331783089 9 16 8.08395556642239 9 17 8.375928322368232 9 18 9.141728277801974 9 19 7.495949541975705 9 20 7.51445762331247 9 21 7.663170916918482 9 22 8.605950881715497 9 23 7.253632269269211 9 24 8.827040173095327 9 25 7.292228472916204 9 26 5.409868626100696 9 27 5.77192388749112 9 28 6.099492917116592 9 29 5.996104661634 9 30 6.0193681586579935 9 31 4.744003965937555 9 32 4.947360477810072 9 33 5.1358213556987415 9 34 4.587584120329904 9 35 4.41731952263568 9 36 5.766194827896559 9 37 6.021337353223906 9 38 5.98714932398104 9 39 7.899516134912179 9 40 2.239410525178645 9 41 9.729986564871396 9 42 10.481910138254982 9 43 10.135622351034652 9 44 11.093602255286278 9 45 10.361977728029643 9 46 12.370853994836526 9 47 12.107920709398833 9 48 12.206656264956443 9 49 12.568074579603831 9 50 12.921880665449123 9 51 11.945722891496207 9 52 11.332054199066544 9 53 12.168366253952346 9 54 11.5075833126099 9 55 10.680165953554123 9 56 9.492042749847705 10 1 3.035266842159743 10 2 3.986445410154708 10 3 3.4366885773567475 10 4 4.6260832949405 10 5 4.253922843402741 10 6 4.547701646090793 10 7 3.8859671407305356 10 8 4.455110540428947 10 9 3.707109595737485 10 10 4.222678689250863 10 11 5.383452543448719 10 12 5.933809418639953 10 13 6.912665722474433 10 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115 9 2.7915690551969448 115 10 3.2587147252869 115 11 0.21002912002529683 115 12 -3.857857248026715 115 13 -4.1571540973453835 115 14 -6.691162568695745 115 15 -6.6863451319850284 115 16 2.0875787548281863 115 17 2.200203684687075 115 18 4.292090596642556 115 19 2.121855339077154 115 20 1.8342040001240605 115 21 5.638714395179349 115 22 6.487291461425645 115 23 5.566132269269211 115 24 6.576134375993874 115 25 5.557627023640844 115 26 5.322006307260111 115 27 4.487503597636049 115 28 5.014347989580358 115 29 4.452626400764437 115 30 5.4106725064840795 115 31 4.2059604876766805 115 32 3.9138459850564473 115 33 3.4184300513509207 115 34 3.962584120329904 115 35 4.668225319737125 115 36 5.4419194655777225 115 37 5.352859092354336 115 38 5.563236280502782 115 39 4.990095845057109 115 40 7.579990235323571 115 41 8.13578366632067 115 42 6.192055065791216 115 43 5.00881075683175 115 44 -4.1790426722499525 115 45 4.577557438174573 115 46 -1.144544555888114 115 47 -8.119434363064935 115 48 -1.2725104017102211 115 49 -5.8848239711208095 115 50 -0.1587352765798613 115 51 0.501882311786062 115 52 1.1735397063129192 115 53 1.8576778481552445 115 54 0.8608441821751143 115 55 1.299731170945428 115 56 4.6478398512969825 116 1 2.671066576320129 116 2 3.2866226365614395 116 3 2.9366885773567475 116 4 3.8411918461146257 116 5 3.5656197862472183 116 6 3.7512904808271657 116 7 3.41941862500169 116 8 3.6695544925778165 116 9 3.158372333885467 116 10 3.359807621461762 116 11 2.5857121801345855 116 12 2.171514336672743 116 13 3.5655234982830315 116 14 4.435203551522836 116 15 -3.7683123450997833 116 16 3.5752836728609765 116 17 5.6612692584575655 116 18 4.143866552926706 116 19 3.9083990549241463 116 20 3.8195182077743333 116 21 5.516105105561863 116 22 6.154299658146957 116 23 5.6580038539686655 116 24 5.523880277633218 116 25 4.8831051657173425 116 26 5.35308554223279 116 27 5.1644161659420575 116 28 5.471315202695109 116 29 5.542790335190666 116 30 5.224880156757305 116 31 5.197080706255921 116 32 5.40803997412749 116 33 4.945752455722502 116 34 4.849879202297114 116 35 5.316107833398327 116 36 6.868832033883731 116 37 6.786602261753245 116 38 6.567334641158521 116 39 7.854849943417765 116 40 9.15649296756401 116 41 8.704089677249634 116 42 6.044514082184659 116 43 1.687772505465631 116 44 4.705861699334747 116 45 5.333704979158178 116 46 -1.8484379985110664 116 47 -6.864994472354551 116 48 -1.7714858115462881 116 49 -4.9135124957109735 116 50 -1.6003336372356003 116 51 1.1077566287259515 116 52 1.2408211270779468 116 53 1.1626641869530587 116 54 1.6852977340876834 116 55 1.0469989305082699 116 56 1.7229764633188402 117 1 1.9409865153212777 117 2 2.619066403240815 117 3 2.866921135496284 117 4 3.135511327624343 117 5 2.785978154527818 117 6 2.925137221200785 117 7 2.4007377252685558 117 8 1.94976798857477 117 9 2.6433132412434546 117 10 2.8207302291628693 117 11 -0.036094910982455986 117 12 -9.124330116243769 117 13 -9.120332391919028 117 14 -2.8549222586182275 117 15 -5.267740480822237 117 16 -5.9996305474973965 117 17 1.2205525218963764 117 18 -2.1798086281636486 117 19 1.304510377836845 117 20 0.6651148528372417 117 21 5.53745470525687 117 22 5.1108379730535525 117 23 5.199368703377736 117 24 5.661890189947364 117 25 4.580398341470296 117 26 5.281793128965539 117 27 5.255914450349223 117 28 4.1800456639989605 117 29 5.510765935648159 117 30 5.356408940592605 117 31 3.722433355893738 117 32 2.867818853273505 117 33 4.143236252901307 117 34 4.401537608701997 117 35 3.055337722837905 117 36 5.588237295035082 117 37 4.2414249838272085 117 38 5.464399071200457 117 39 5.716840031103622 117 40 7.653633646176285 117 41 5.779194519033851 117 42 4.657171344860984 117 43 -3.1326621113853044 117 44 -1.926620191629798 117 45 4.50488301956992 117 46 -0.62274223030672 117 47 -0.24394986694090193 117 48 -1.7623359831055687 117 49 -0.6348239711208095 117 50 0.5026019327224631 117 51 3.5881226218635796 117 52 2.539334279956332 117 53 2.281611956682376 117 54 3.210650383725504 117 55 3.2551575275345748 117 56 5.9986150450954305 118 1 1.0868638937567923 118 2 2.7853807090900062 118 3 2.270021910690083 118 4 3.117893286750487 118 5 2.6351677246476157 118 6 3.214777813166961 118 7 2.6206108753742683 118 8 2.772882858201264 118 9 2.8483872370151246 118 10 3.364775331347502 118 11 0.09639275638892997 118 12 -6.566190581360047 118 13 -8.376851067042356 118 14 -10.513132265665444 118 15 -10.686345131985028 118 16 -7.344239426989997 118 17 0.6130824725658641 118 18 -3.8556366760847176 118 19 0.6540523087741263 118 20 0.5368555152755761 118 21 5.4550022739672315 118 22 6.40963994627413 118 23 5.193026208663149 118 24 7.123482860842358 118 25 5.737551266065086 118 26 5.74056691332072 118 27 5.8549278400602915 118 28 5.359044959277327 118 29 5.376868825006859 118 30 6.380369476181048 118 31 4.558233214949407 118 32 5.309679318389783 118 33 4.766914899835765 118 34 5.223947756693537 118 35 3.8481495621613675 118 36 6.165404314062567 118 37 5.189980304475547 118 38 5.692024159290657 118 39 6.406762511723773 118 40 8.019384174717512 118 41 5.347904878441884 118 42 6.6162974900336415 118 43 1.910325908346902 118 44 1.3228512671439887 118 45 6.505587741204877 118 46 -1.2562869801305396 118 47 0.45442927329870386 118 48 2.1157471740473532 118 49 0.8803275440307061 118 50 2.1537647234201387 118 51 4.47536716027091 118 52 5.4216457669189815 118 53 4.363359666337065 118 54 6.561601757932692 118 55 6.580034201248456 118 56 10.019051972509104 119 1 1.8378520360492843 119 2 2.6615335417645767 119 3 2.5453842295306615 119 4 2.932781297290674 119 5 3.0053917035672484 119 6 2.973671093799368 119 7 2.879504156006675 119 8 2.1813150057638495 119 9 2.340482098675203 119 10 2.8166857397796505 119 11 -0.9167824741776052 119 12 -9.805321016142656 119 13 -5.573820764012051 119 14 -7.482829235362413 119 15 -7.664606001550247 119 16 -7.575464723432685 119 17 -0.1965354457477062 119 18 -4.57566302654585 119 19 -1.2359345159953072 119 20 -2.13771628973101 119 21 2.735634685034423 119 22 4.4465305918604265 119 23 2.181168501153266 119 24 2.138634375993874 119 25 4.009619777264032 119 26 4.511317901463009 119 27 4.387865916476628 119 28 4.041521902623835 119 29 4.1917568355470465 119 30 3.6860348253246578 119 31 3.359945994923063 119 32 3.2662010575202203 119 33 2.911183674539327 119 34 3.3919319464168574 119 35 1.7216673487226366 119 36 3.1357600452878636 119 37 3.4416272082963673 119 38 3.0741058457201724 119 39 3.5661828015788473 119 40 4.746656901990239 119 41 2.8894068547264666 119 42 2.583359413617302 119 43 -5.857131272153758 119 44 -4.449876005583285 119 45 1.3764704816528344 119 46 -2.3103054254533326 119 47 -2.4138184210359483 119 48 -5.764358227797178 119 49 -3.417432666772985 119 50 -2.7810178852755136 119 51 0.9819547755541755 119 52 1.7885759381969777 119 53 2.1031488626479984 119 54 0.7177282401461298 119 55 2.491760156452674 119 56 3.5355210107172717 120 1 1.9911701138524833 120 2 2.7175976149911207 120 3 1.7524780510409599 120 4 3.062071915139647 120 5 2.8257578362903644 120 6 3.2219548466597843 120 7 3.285682645709194 120 8 2.912436287228381 120 9 3.08762168677589 120 10 2.188539286690407 120 11 -1.1101463185711964 120 12 -8.039874791886362 120 13 -5.205399711380473 120 14 -6.667039761678204 120 15 -0.923187237248186 120 16 -11.451894929382341 120 17 -1.859006841628716 120 18 -7.117997122655691 120 19 -3.543714836361442 120 20 -2.048471438472429 120 21 2.041126675881106 120 22 4.187949356162488 120 23 2.7097726201464027 120 24 4.164950165467559 120 25 4.0565305324127685 120 26 5.309944903751344 120 27 4.437065001144816 120 28 4.6261900948435155 120 29 5.7947316639223345 120 30 5.077339173150744 120 31 4.269556978904756 120 32 3.6781003710213653 120 33 5.11141250749127 120 34 4.429689383487801 120 35 5.544321810965201 120 36 6.246744026981229 120 37 5.5217187414771445 120 38 5.997446806818569 120 39 5.9506221608465815 120 40 8.983499007253396 120 41 -5.6712338775389775 120 42 -3.9921554605245753 120 43 -6.916627839659478 120 44 -13.528823374004338 120 45 -4.981653088141218 120 46 -10.67414981904601 120 47 -8.190706292889496 120 48 -6.723168296447064 120 49 -13.371666076383967 120 50 -6.056761592369336 120 51 -1.9082054075121846 120 52 -2.656504153336204 120 53 -1.6565765378096664 120 54 -2.0580154669476904 120 55 -1.0116723378265036 120 56 1.0469626583145235 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1512.0 5 262.0 6 123.0 7 222.0 8 401.0 9 375.0 10 265.0 11 257.0 12 180.0 13 147.0 14 193.0 15 216.0 16 256.0 17 306.0 18 427.0 19 544.0 20 629.0 21 739.0 22 902.0 23 1105.0 24 1194.0 25 1407.0 26 1471.0 27 1625.0 28 1747.0 29 1762.0 30 2118.0 31 2343.0 32 2511.0 33 2872.0 34 3119.0 35 3618.0 36 3810.0 37 4054.0 38 3790.0 39 2452.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 100.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 0.0 100.0 6 0.0 0.0 0.0 100.0 7 98.60276994729746 0.817093598071659 0.0980512317685991 0.4820852228622789 8 99.20946194386568 0.43101687298280017 0.09192302978306165 0.26759815336846837 9 98.12677466140993 0.9172062999203318 0.3697424059812473 0.5862766326884972 10 61.19009682559137 21.72243330473506 8.144380438779262 8.94308943089431 11 41.51039751603546 23.953098827470686 18.394819626588227 16.141684029905626 12 31.776770029006823 15.304163091882176 22.6294071985946 30.2896596805164 13 24.755893287576093 15.17138538219553 26.00400375863055 34.06871757159783 14 23.334150426931405 20.960493524533234 28.347019651101036 27.358336397434325 15 26.072688489621793 20.448765280170644 25.127163836245796 28.35138239396177 16 32.28879213425727 20.52901616892541 23.245640931297398 23.93655076551992 17 29.37498723004311 20.84056964223689 26.536992011114968 23.24745111660503 18 26.500388119459085 20.99522000245128 26.567798341299998 25.936593536789637 19 31.181675146611088 22.145936778438465 20.64202374384438 26.03036433110607 20 29.976274237094003 23.05898715536284 23.11012026507404 23.854618342469116 21 29.333660170772564 21.105527638190953 25.303345998284104 24.257466192752382 22 26.492217183478367 19.58369081178249 25.969277280712504 27.95481472402664 23 25.456551047922538 22.786697716223394 23.434244392695184 28.322506843158884 24 24.36876940676581 22.77741461023043 26.16849158359209 26.68532439941167 25 26.910584053441195 25.6726113868971 22.32436518150804 25.092439378153664 26 27.29000613120785 22.893930104230535 23.153484569793584 26.66257919476804 27 24.909046314842822 22.029186935371786 22.368474839553613 30.69329191023178 28 25.63583992155421 23.972952544381116 23.617495046066477 26.773712487998203 29 30.928320104737743 21.577612306685214 21.023238687505113 26.470828901071926 30 27.92404235313356 21.955357507869667 25.595846449450143 24.524753689546625 31 28.930753356081812 24.662348542122146 22.283974581639114 24.122923520156924 32 32.07273470221678 22.653999387067117 21.28920216569619 23.98406374501992 33 28.73678398331254 22.468762142375102 23.462647497903845 25.331806376408515 34 27.005790755253628 24.98004951811913 24.998465347547626 23.01569437907962 35 26.977048643783498 24.436443971689236 27.09160086732398 21.494906517203287 36 27.82603364058125 25.068977498007317 22.91892333789777 24.186065523513665 37 27.949688107168424 23.56887207280908 25.209121587074346 23.272318232948155 38 29.543826617276874 25.63903157808567 22.164009577799153 22.653132226838302 39 27.817319098457887 23.399599132817933 22.139730846320614 26.64335092240357 40 22.934816187828236 19.347132118216457 21.785603954278653 35.932447739676654 41 19.88030131049427 15.791971932721083 20.602620988546075 43.72510576823857 42 24.966209297562976 23.41798074953922 23.569526930165882 28.046283022731927 43 22.048582664263478 19.4228248402425 26.738898902179255 31.78969359331476 44 24.118129614438065 20.963904840032814 23.359310910582444 31.558654634946677 45 25.365833691492192 22.733877734798718 26.17424940136305 25.726039172346045 46 23.851763838243127 24.417175400499858 25.62789363707133 26.103167124185685 47 20.530290592677723 21.950418650467903 26.169758660318504 31.34953209653587 48 25.167606900498736 23.19924781293435 26.367427029678687 25.265718256888235 49 23.364390293846625 22.516637657417835 24.73430940923518 29.38466263950036 50 26.25 21.937397708674304 27.13379705400982 24.678805237315878 51 24.604637823664373 23.033431661750246 26.987053425106524 25.374877089478858 52 24.085440707095508 22.24813814551109 28.118094770439477 25.548326376953923 53 24.519841107334454 21.124943691387855 27.75502682337524 26.60018837790245 54 25.56297084834589 23.311087454962333 26.53128070750082 24.59466098919096 55 24.291887685092263 23.1060683638151 26.661478280459583 25.940565670633053 56 25.047593703301878 21.915620969888028 27.65757098114675 25.379214345663343 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 2.0 7 4.0 8 3.5 9 3.0 10 3.5 11 4.0 12 5.0 13 9.0 14 12.0 15 27.0 16 42.0 17 47.5 18 53.0 19 54.5 20 56.0 21 55.0 22 61.0 23 68.0 24 76.0 25 84.0 26 90.5 27 97.0 28 100.5 29 131.0 30 158.0 31 180.5 32 203.0 33 244.0 34 285.0 35 291.5 36 298.0 37 403.5 38 591.0 39 673.0 40 720.0 41 767.0 42 810.5 43 854.0 44 983.0 45 1112.0 46 1368.0 47 2169.5 48 2715.0 49 2684.0 50 2653.0 51 2483.0 52 2313.0 53 2476.5 54 2767.0 55 2894.0 56 3101.0 57 3308.0 58 3274.5 59 3241.0 60 3377.0 61 3513.0 62 3688.0 63 3755.0 64 3647.0 65 3224.0 66 2801.0 67 2630.0 68 2459.0 69 2107.0 70 1755.0 71 1502.0 72 1145.5 73 1042.0 74 923.5 75 805.0 76 620.5 77 436.0 78 325.5 79 168.5 80 122.0 81 135.5 82 149.0 83 94.5 84 40.0 85 26.5 86 13.0 87 7.0 88 1.0 89 1.0 90 1.0 91 1.0 92 0.5 93 0.0 94 1.0 95 2.0 96 4.0 97 3.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.002042733995179148 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.40446133104547133 16 0.06741022184091187 17 0.022470073946970627 18 0.0 19 0.030641009927687216 20 0.12664950770110717 21 0.0 22 0.0 23 0.0 24 0.004085467990358296 25 0.006128201985537444 26 0.04902561588429955 27 0.05719655186501613 28 0.006128201985537444 29 0.14299137966254033 30 0.06536748784573274 31 0.026555541937328918 32 0.01838460595661233 33 0.11235036973485313 34 0.16954692159986928 35 0.13890591167218205 36 0.05106834987947869 37 0.12052130571556972 38 0.18588879356130245 39 0.12256403971074886 40 0.81505086407648 41 1.0193242635943947 42 0.25329901540221433 43 0.26555541937328925 44 0.3962903950647547 45 0.18997426155166072 46 0.28598275932508066 47 0.46165788291048737 48 0.06128201985537443 49 0.24308534542631857 50 0.15116231564325694 51 0.2818972913347224 52 0.15933325162397352 53 0.23695714344078114 54 0.21652980348898965 55 0.25942721738775176 56 0.2104016015034522 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 48954.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.17355068023042 #Duplication Level Percentage of deduplicated Percentage of total 1 89.38589434164655 68.08840952731136 2 6.454813622955216 9.833721452792417 3 1.8235451863770447 4.167177350165462 4 0.748189863234111 2.279691138619929 5 0.48806650576562083 1.8588879356130246 6 0.2172164119066774 0.9927687216570659 7 0.17162778224725128 0.9151448298402581 8 0.12872083668543846 0.7844098541487928 9 0.06704210244033253 0.45961514891530825 >10 0.471976401179941 7.086244229276463 >50 0.026816840976133013 1.3972300527025372 >100 0.016090104585679808 2.1366997589573886 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ACACCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 406 0.8293500020427339 No Hit ACACCCGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT 143 0.2921109613106181 No Hit ACACCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 132 0.2696408873636475 No Hit ACACCCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 131 0.26759815336846837 No Hit ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 127 0.25942721738775176 No Hit ACACCCGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC 107 0.2185725374841688 No Hit ACACCCGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA 89 0.18180332557094417 No Hit ACACCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 81 0.16546145360951098 No Hit ACACCCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC 77 0.15729051762879437 No Hit ACACCCGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC 75 0.15320504963843606 No Hit ACACCCGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC 67 0.1368631776770029 No Hit ACACCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 67 0.1368631776770029 No Hit ACACCCGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT 63 0.12869224169628632 No Hit ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 56 0.11439310373003227 No Hit ACACCCGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC 55 0.11235036973485313 No Hit ACACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGT 54 0.11030763573967398 No Hit ACACCCGGGAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGA 50 0.10213669975895738 No Hit ACACCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG 50 0.10213669975895738 No Hit ACACCCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT 49 0.10009396576377826 No Hit ACACCCGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG 49 0.10009396576377826 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.004085467990358295 0.0 0.0 0.0 0.0 12 0.004085467990358295 0.0 0.0 0.0 0.0 13 0.006128201985537443 0.0 0.0 0.0 0.0 14 0.010213669975895739 0.0 0.0 0.0 0.0 15 0.014299137966254035 0.0 0.0 0.0 0.0 16 0.01634187196143318 0.0 0.0 0.0 0.0 17 0.01634187196143318 0.0 0.0 0.0 0.0 18 0.01634187196143318 0.0 0.0 0.0 0.0 19 0.020427339951791478 0.0 0.0 0.0 0.0 20 0.024512807942149772 0.0 0.0 0.0 0.0 21 0.024512807942149772 0.0 0.0 0.0 0.0 22 0.02655554193732892 0.0 0.0 0.0 0.0 23 0.02655554193732892 0.0 0.0 0.0 0.0 24 0.02859827593250807 0.0 0.0 0.0 0.0 25 0.030641009927687216 0.0 0.0 0.0 0.0 26 0.03472647791804551 0.0 0.0 0.0 0.0 27 0.03472647791804551 0.0 0.0 0.0 0.0 28 0.03881194590840381 0.0 0.0 0.0 0.0 29 0.03881194590840381 0.0 0.0 0.0 0.0 30 0.03881194590840381 0.0 0.0 0.0 0.0 31 0.03881194590840381 0.0 0.0 0.0 0.0 32 0.040854679903582956 0.0 0.0 0.0 0.0 33 0.040854679903582956 0.0 0.0 0.0 0.0 34 0.0428974138987621 0.0 0.0 0.0 0.0 35 0.044940147893941254 0.0 0.0 0.0 0.0 36 0.044940147893941254 0.0 0.0 0.0 0.0 37 0.0469828818891204 0.0 0.0 0.0 0.0 38 0.0469828818891204 0.0 0.0 0.0 0.0 39 0.049025615884299545 0.0 0.0 0.0 0.0 40 0.05515381786983699 0.0 0.0 0.0 0.0 41 0.05719655186501614 0.0 0.0 0.0 0.0 42 0.059239285860195286 0.0 0.0 0.0 0.0 43 0.06332475385055358 0.0 0.0 0.0 0.0 44 0.06332475385055358 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCAATCC 25 1.9120784E-5 50.62331 41 CGTCTCA 20 3.8979645E-4 50.623306 41 TTCAATC 20 3.938242E-4 50.518173 40 CTCGTCT 25 1.9358016E-5 50.51817 39 GTTCAAT 25 1.9358016E-5 50.51817 39 GCTCGTC 25 1.9358016E-5 50.51817 38 AGACCTT 15 0.00808075 50.465767 37 CAGGACT 30 9.772302E-7 50.361282 35 CACGATT 15 0.0082143005 50.25723 47 CGCCCAA 30 9.913128E-7 50.25723 47 CCACAAC 15 0.008247944 50.205364 13 ACAACTA 15 0.008247944 50.205364 15 CTCCACG 15 0.008247944 50.205364 44 GACTTAA 15 0.008247944 50.205364 10 ATCCGCC 35 4.986032E-8 50.205364 44 CCCCAAC 20 4.061062E-4 50.205364 13 GACGAGA 15 0.008247944 50.205364 13 GCCACAA 15 0.008247944 50.205364 12 CGCCACA 15 0.008247944 50.205364 42 AGGTCAT 15 0.008315536 50.101955 49 >>END_MODULE