FastQCFastQC Report
Fri 17 Jun 2016
SRR1526029_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526029_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012
Sequences flagged as poor quality0
Sequence length40
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCCGGGGTATTTATTTACTGTTGATTTGTTTGTGTCT80.7905138339920948No Hit
ACACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC70.691699604743083No Hit
ACACCCGGGGTGGGGGTTAGCCTGGAAGTTTGGGAGAAGG30.2964426877470355No Hit
ACACCCGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCC30.2964426877470355No Hit
ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC30.2964426877470355No Hit
ACACCCGGGAGAGGGATTGCACATCCATATTATTATGATC20.1976284584980237No Hit
ACACCCGGGGTGGCATCAACGCGAAAAAAAAAAAAAAAAA20.1976284584980237No Hit
ACACCCGGGAATCCGCAGCGGCTTCTGCGCCGATCAGACT20.1976284584980237No Hit
ACACCCGGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAG20.1976284584980237No Hit
ACACCCGGGGTATTTATTTACTGTTGCTTTGTTTGTGTCT20.1976284584980237No Hit
ACACCCGGGAAGCAGTGGTATCAACGCCGAGTCGACTTTT20.1976284584980237No Hit
ACACCCGGGCAGAGTCGACCTTTTTTTTTTTTTTTTTTTT20.1976284584980237No Hit
ACACCCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCT20.1976284584980237No Hit
ACACCCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCC20.1976284584980237No Hit
ACACCCGGGGGAAGGATTTTAGTATTTCTGGTCTGTTGAC20.1976284584980237No Hit
ACACCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC20.1976284584980237No Hit
ACACCCGGTCCGCATCGTTTTTTTTTTTTTTTTTTTTTTT20.1976284584980237No Hit
ACACCCGGGGGCCTGTGGCGATCGCGTGTGAGGTGAGGTC20.1976284584980237No Hit
ACACCCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCC20.1976284584980237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGGG501.8189894E-1133.654
CCCGGGA200.001748940333.654
ACACCCG900.033.651
ACCCGGG800.033.653
CACCCGG850.033.652
CCGGGGG259.7661825E-533.655
Produced by FastQC (version 0.11.5)