FastQCFastQC Report
Fri 17 Jun 2016
SRR1526027_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526027_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1923
Sequences flagged as poor quality0
Sequence length40
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT231.1960478419136766No Hit
ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC221.1440457618304731No Hit
ACACCCGGGGGGGAGGTAGTGACGAAAAATAACAATACAG60.31201248049922No Hit
ACACCCCAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.31201248049922No Hit
ACACCCGGGATGCCACAACTAGATACATCAACATGATTTA50.26001040041601664No Hit
ACACCCGGGACTAATCCTAGCCCTAGCCCTACACAAATAT50.26001040041601664No Hit
ACACCCCAAAAAAAAAAAAAACAAAAAAAAAAAAAAAAAA40.20800832033281333No Hit
ACACCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG40.20800832033281333No Hit
ACACCCGGGATGCCATCTACCTTCTTCAACCTCACCATAG40.20800832033281333No Hit
ACACCCGGGATATTATTTGATCTTATCTTGGATCCTTTTG40.20800832033281333No Hit
ACACCCACCAAAACAAAAAAAAAAAAAAAAAAAAAAAAAA40.20800832033281333No Hit
ACACCCCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.15600624024961No Hit
ACACCCGGGGAGTCCCCATGATACTCCCGGGTGTTCTGAG30.15600624024961No Hit
ACACCCGGGATCGAAATTCCTACCGGTGTCAAAGTATTTA30.15600624024961No Hit
ACACCCGGGGATCTCCAGCTATACTATGAGCCTTAGGCTT30.15600624024961No Hit
ACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.15600624024961No Hit
ACACCCGGGATGGCCTACCCATTCCAACTTGGTCTACAAG30.15600624024961No Hit
ACACCCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGC30.15600624024961No Hit
ACACCCGGGGGGAGTAGTTGTGGAAAGCAGGCCTCTGTAC30.15600624024961No Hit
ACACCCGGGGTGACGACTCAGTGATCCAGCTCCTGAACCC30.15600624024961No Hit
ACACCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA30.15600624024961No Hit
ACACCCGGGGTGACCATAACCTTTGCTGTGCTGAGTAAAG20.10400416016640666No Hit
ACACCCGGGCCTTTAAAATTATTTAAATCTAGATTTCAGG20.10400416016640666No Hit
ACACCCGGGGGGTTTTTGTTTGTTAGTTTGTTTCTTGTCC20.10400416016640666No Hit
ACACCCGGGAATCTTCTTAGTCAAATGCTGGAAGCCCCAG20.10400416016640666No Hit
ACACCCGGGATGCCACAAATAGATACATCAACATGATTTA20.10400416016640666No Hit
ACACCCGGGACGCACGGCCGGTACAGTGAAACTGCGAATG20.10400416016640666No Hit
ACACCCGGGAGATGAAGTTTTTGTTACTTGTCTCTAAAGT20.10400416016640666No Hit
ACACCCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT20.10400416016640666No Hit
ACACCCGGGTACCTGGTTGATACTGCCAGTAGCATATGCT20.10400416016640666No Hit
ACACCCGGGGATAAGGTGCTCTTCTTTTCCTTCCCCTCAA20.10400416016640666No Hit
ACACCCGGGAACTCATAACTATACACTGTAATGCAAGCAT20.10400416016640666No Hit
ACACCCGGGGGACTTTTGAAGTGTTGGAATTTTTGAAGAC20.10400416016640666No Hit
ACACCCGGGGTGTGTGTGTGTGTGTGTGAGAGAGAGAGAG20.10400416016640666No Hit
ACACCCGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACT20.10400416016640666No Hit
ACACCCGGGATTCGTAGGAGTAAACATAACATTCTTCCCT20.10400416016640666No Hit
ACACCCGGGGCCTCTTCGAGATCATTGGGGTCAAGAGCCA20.10400416016640666No Hit
ACACCCCAACAAAAAAAAAAAAAAAAAAAAAATAAAAAAA20.10400416016640666No Hit
ACACCCAAACAAACAAAAAAAAAAAAAAAAAAAAAAAAAA20.10400416016640666No Hit
ACACCCGGGGAAGTCTCTTAACCCAGAACTGGGCACTGAC20.10400416016640666No Hit
ACACCCGGGGATTGCTTGCTCCTGGAAACTATAAACTTAC20.10400416016640666No Hit
ACACCCGGGGTGACATTGCAACTCCAATCAGTTAGTAAGC20.10400416016640666No Hit
ACACCCGGGGGATTAGATGTAGACACACGAGCTTACTTTA20.10400416016640666No Hit
ACACCCGGGGAACTTGCTCACCTTCAATGTGGGCTATCAT20.10400416016640666No Hit
ACACCCGGGGTCGGGTTCTATTTGGGAGTGACTGCGTTTT20.10400416016640666No Hit
ACACCCGGGGTTCACTGATTCCCATTATTTTCAGGCTTCA20.10400416016640666No Hit
ACACCCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGA20.10400416016640666No Hit
ACACCCGGGGTGTTCTTTATTAATATCCTAACACTCCTCG20.10400416016640666No Hit
ACACCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAG20.10400416016640666No Hit
ACACCCGGGTCTCCCTATTCGATAAAGATGGTGACGGCAC20.10400416016640666No Hit
ACACCCGGGAGGAAGGGAGGTTTGCCTGTTGTGCTTGCAA20.10400416016640666No Hit
ACACCCGGGGGTTCGATTCCGGAGAGGGAGCCTGAGAAAC20.10400416016640666No Hit
ACACCCGGGAGGGATGTTCCCGGCTCAGGAGGAGGCTGAC20.10400416016640666No Hit
ACACCCGGGGGAGCTGGAGAAATGGCTCAGTGCTTAAGAG20.10400416016640666No Hit
ACACCCGGGGTTGTTTTGGATTCCTTTGGGTTTTGCTTTG20.10400416016640666No Hit
ACACCCGGGGTTTTACGTTTTTGTTTTTTGGCTCCTGGAT20.10400416016640666No Hit
ACACCCGGGGGTCTCTGCATCTGTGACTAACACAGAAACA20.10400416016640666No Hit
ACACCCGGGGCTCTTTTCCTCCTCTCCAGCTCCGGCGCCG20.10400416016640666No Hit
ACACCCGGGACCCTTTGGAGCTGCAGCTGTGCCCGGCACA20.10400416016640666No Hit
ACACCCGGGATCAATATTTTCACAACCTCAATCTTATTAA20.10400416016640666No Hit
ACACCCGGGGGGGCTGGTGAGCTGGCTCAGTGGGTAAGAG20.10400416016640666No Hit
ACACCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA20.10400416016640666No Hit
ACACCCGGGACTAATCCTAGCACTAGCCCTACACAAATAT20.10400416016640666No Hit
ACACCCGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT20.10400416016640666No Hit
ACACCCGGGGATCGTTTCAAGGGTGGGATTCATCCCTCAG20.10400416016640666No Hit
ACACCCGGGGTAGCCCATTTCCACTATGTTCTATCAATGG20.10400416016640666No Hit
ACACCCGGGGGGCTGGTGAGATGGCTCAGCGGTTAAGAGC20.10400416016640666No Hit
ACACCCGGGAGCCCACAGAGCTAATGGTACCTCAATCTAA20.10400416016640666No Hit
ACACCCGGGGTGTTTGCTATCATAGCAGGATTTGTTCACT20.10400416016640666No Hit
ACACCCGGGGGGTGAGTTCTGCTACTGCAGTGTGTCCATA20.10400416016640666No Hit
ACACCCGGGCCTGTGAAGTCTGACAACAAGGATGATGACA20.10400416016640666No Hit
ACACCCGGGGGCTTTCTGAAGATGGCGGCGGCTAAGGACG20.10400416016640666No Hit
ACACCCGGGAGGGTTCGATTCCGGAGAGGGAGCCTGAGAA20.10400416016640666No Hit
ACACCCGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT20.10400416016640666No Hit
ACACCCGGGAACTTAATTATGTCTCACTATGGTTTCTACA20.10400416016640666No Hit
ACACCCGGGGGCTACCACATCCAAGGAAGGCAGCAGGCGC20.10400416016640666No Hit
ACACCCGGGGGAAAAAACCTTTAATAGTGAATAATTAACA20.10400416016640666No Hit
ACACCCGGGATTCTTAGGGAAGAGAGCTTTGAACGAAAAG20.10400416016640666No Hit
ACACCCGGGGCAGGATTTGTTCACTGATTCCCATTATTTT20.10400416016640666No Hit
ACACCCGGGGGAGAGGGAGCCTGAGAAACGGCTACCACAT20.10400416016640666No Hit
ACACCCGGGGAGTCGACTTTTTTTTTTGTTTGTTTTTTTT20.10400416016640666No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGG200.002148233834.0000046
CCCGGGT200.002148233834.0000044
CCGGGGT200.002148233834.0000045
CCGGGGG355.147558E-734.0000045
CCGGGAG200.002148233834.0000045
CCCGGGA555.456968E-1234.04
ACACCCG1700.034.01
ACCCGGG1700.034.03
CACCCGG1700.034.02
CCCGGGG850.032.04
Produced by FastQC (version 0.11.5)