##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526027_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1923 Sequences flagged as poor quality 0 Sequence length 40 %GC 51 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.625585023400937 30.0 22.0 34.0 4.0 36.0 2 19.931357254290173 22.0 4.0 33.0 4.0 36.0 3 19.00988039521581 21.0 4.0 30.0 4.0 35.0 4 19.003640145605825 20.0 4.0 32.0 4.0 36.0 5 18.213208528341134 17.0 4.0 31.0 4.0 36.0 6 18.319292771710867 19.0 4.0 31.0 4.0 35.0 7 18.06916276651066 18.0 4.0 31.0 4.0 35.0 8 18.988039521580863 23.0 4.0 32.0 4.0 35.0 9 18.40353614144566 19.0 4.0 33.0 4.0 35.0 10 15.846073842953718 10.0 4.0 30.0 4.0 34.0 11 14.25585023400936 4.0 4.0 27.0 4.0 33.0 12 13.745709828393135 4.0 4.0 26.0 4.0 33.0 13 12.960478419136766 4.0 4.0 23.0 4.0 32.0 14 13.098283931357255 4.0 4.0 25.0 4.0 33.0 15 12.846593863754551 4.0 4.0 25.0 4.0 33.0 16 10.880395215808633 4.0 4.0 19.0 4.0 29.0 17 10.479459178367135 4.0 4.0 18.0 4.0 28.0 18 9.796151846073842 4.0 4.0 15.0 4.0 26.0 19 9.927717108684348 4.0 4.0 14.0 4.0 28.0 20 10.390015600624025 4.0 4.0 17.0 4.0 29.0 21 8.88507540301612 4.0 4.0 12.0 4.0 24.0 22 8.711388455538222 4.0 4.0 11.0 4.0 23.0 23 9.18148725949038 4.0 4.0 12.0 4.0 26.0 24 8.505980239209569 4.0 4.0 10.0 4.0 22.0 25 9.331773270930837 4.0 4.0 14.0 4.0 26.0 26 9.648985959438377 4.0 4.0 13.0 4.0 28.0 27 9.795111804472178 4.0 4.0 14.0 4.0 29.0 28 9.919396775871034 4.0 4.0 15.0 4.0 29.0 29 8.78783151326053 4.0 4.0 11.0 4.0 25.0 30 9.273530941237649 4.0 4.0 13.0 4.0 26.0 31 10.46281851274051 4.0 4.0 17.0 4.0 31.0 32 10.21476859074363 4.0 4.0 15.0 4.0 31.0 33 10.15080603224129 4.0 4.0 15.0 4.0 30.0 34 10.36557462298492 4.0 4.0 14.0 4.0 31.0 35 10.281851274050963 4.0 4.0 13.0 4.0 32.0 36 10.183047321892875 4.0 4.0 4.0 4.0 32.0 37 9.997399895995839 4.0 4.0 4.0 4.0 32.0 38 9.858554342173687 4.0 4.0 4.0 4.0 32.0 39 9.556942277691107 4.0 4.0 4.0 4.0 31.0 40 9.526261050442018 4.0 4.0 4.0 4.0 31.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 10.351774042950513 1 2 -12.477637721755372 1 3 -11.490627917833804 1 4 -11.540546218487393 1 5 -11.44623015873016 1 6 -11.581500933706817 1 7 -11.258204948646123 1 8 -12.021358543417367 1 9 -11.797992530345471 1 10 -10.047373949579832 1 11 -8.413001867413632 1 12 -7.534792250233426 1 13 -7.389915966386555 1 14 -7.1286764705882355 1 15 -7.294386087768441 1 16 -5.422829131652662 1 17 -4.79264705882353 1 18 -4.341433239962653 1 19 -3.9377217553688144 1 20 -4.2453081232493 1 21 -4.4077264239028935 1 22 -3.9395774976657325 1 23 -4.403373015873017 1 24 -4.395739962651726 1 25 -4.911986461251168 1 26 -5.2864845938375336 1 27 -4.860889355742298 1 28 -4.994887955182074 1 29 -4.553933239962651 1 30 -4.250863678804855 1 31 -5.336367880485527 1 32 -5.013211951447245 1 33 -4.978174603174603 1 34 -5.07047152194211 1 35 -5.223295985060691 1 36 -5.147233893557424 1 37 -5.021708683473392 1 38 -5.2804971988795515 1 39 -4.96078431372549 1 40 -5.3533613445378165 2 1 9.066059757236228 2 2 8.66521942110177 2 3 9.366514939309054 2 4 8.03088235294118 2 5 8.410912698412698 2 6 2.84707049486461 2 7 7.31322362278245 2 8 1.692927170868348 2 9 -10.797992530345471 2 10 -5.190231092436974 2 11 -6.413001867413632 2 12 -4.534792250233426 2 13 -1.8184873949579838 2 14 -5.1286764705882355 2 15 -5.1515289449112975 2 16 -4.279971988795519 2 17 -3.506932773109244 2 18 1.3728524743230608 2 19 1.776563958916899 2 20 -0.3881652661064434 2 21 0.5922735760971065 2 22 0.34613678804855397 2 23 -0.5462301587301601 2 24 -0.9671685340802973 2 25 0.8022992530345459 2 26 -7.703081232470765E-4 2 27 2.2819677871148443 2 28 0.576540616246497 2 29 0.7317810457516352 2 30 2.177707749766574 2 31 1.9493464052287592 2 32 3.272502334267042 2 33 2.7361111111111107 2 34 1.072385620915032 2 35 2.0624183006535954 2 36 1.2813375350140053 2 37 1.1211484593837504 2 38 3.1480742296918773 2 39 3.324929971988796 2 40 -0.9247899159663877 3 1 7.351774042950513 3 2 16.02236227824463 3 3 13.676038748832864 3 4 9.959453781512607 3 5 11.05376984126984 3 6 4.751832399626515 3 7 2.908461718020545 3 8 5.311974789915965 3 9 10.53534080298786 3 10 -4.214040616246498 3 11 -0.5796685340802981 3 12 3.6318744164332397 3 13 4.276750700280111 3 14 -2.4620098039215694 3 15 3.8722805788982253 3 16 5.243837535014004 3 17 8.540686274509802 3 18 4.991900093370681 3 19 7.8956115779645195 3 20 4.921358543417366 3 21 4.42560690943044 3 22 4.393755835667601 3 23 5.429960317460317 3 24 2.27092670401494 3 25 1.7546802054154984 3 26 2.7135154061624664 3 27 0.13911064425770192 3 28 2.505112044817926 3 29 2.4460667600373487 3 30 2.9158029878618112 3 31 1.6636321195144728 3 32 2.9867880485527554 3 33 1.3551587301587311 3 34 3.2628618113912236 3 35 -2.389962651727358 3 36 4.5194327731092425 3 37 -2.855042016806725 3 38 -2.7804971988795515 3 39 -0.1274509803921564 3 40 -0.5200280112044826 5 1 10.351774042950513 5 2 20.52236227824463 5 3 -4.4906279178338036 5 4 21.459453781512607 5 5 2.5537698412698404 5 6 15.418499066293183 5 7 -1.2582049486461226 5 8 -1.0213585434173673 5 9 14.202007469654529 5 10 18.952626050420168 5 11 24.586998132586366 5 12 7.465207749766574 5 13 8.610084033613445 5 14 18.871323529411764 5 15 7.705613912231559 5 16 2.5771708683473378 5 17 1.2073529411764703 5 18 15.658566760037347 5 19 5.062278244631186 5 20 9.7546918767507 5 21 22.592273576097107 5 22 2.0604225023342675 5 23 2.596626984126983 5 24 21.604260037348276 5 25 23.088013538748832 5 26 3.7135154061624664 5 27 4.139110644257702 5 28 24.005112044817928 5 29 24.446066760037347 5 30 17.749136321195145 5 31 21.663632119514475 5 32 11.986788048552755 5 33 8.021825396825397 5 34 18.92952847805789 5 35 23.77670401493931 5 36 5.852766106442576 5 37 25.978291316526608 5 38 15.719502801120449 5 39 9.03921568627451 5 40 25.646638655462183 6 1 8.018440709617181 6 2 -3.6443043884220376 6 3 -1.8239612511671375 6 4 -1.8738795518207265 6 5 -5.112896825396826 6 6 -1.0815009337068169 6 7 -2.7582049486461226 6 8 0.6453081232493005 6 9 0.20200746965452865 6 10 -4.880707282913166 6 11 2.253664799253034 6 12 3.7985410830999076 6 13 5.776750700280113 6 14 6.537990196078432 6 15 6.705613912231559 6 16 8.91050420168067 6 17 10.70735294117647 6 18 8.49190009337068 6 19 11.395611577964518 6 20 8.921358543417368 6 21 4.42560690943044 6 22 4.0604225023342675 6 23 6.763293650793649 6 24 2.27092670401494 6 25 4.421346872082166 6 26 6.2135154061624664 6 27 1.305777310924368 6 28 2.505112044817926 6 29 4.779400093370683 6 30 7.415802987861811 6 31 4.830298786181139 6 32 8.653454715219423 6 33 5.355158730158731 6 34 8.262861811391222 6 35 6.776704014939309 6 36 3.5194327731092425 6 37 2.311624649859942 6 38 4.7195028011204485 6 39 4.205882352941176 6 40 3.1466386554621835 7 1 13.018440709617177 7 2 18.355695611577964 7 3 13.509372082166196 7 4 13.626120448179275 7 5 19.55376984126984 7 6 9.418499066293183 7 7 0.40846171802054343 7 8 8.311974789915965 7 9 13.702007469654529 7 10 10.7859593837535 7 11 1.420331465919702 7 12 9.298541083099906 7 13 11.776750700280113 7 14 5.3713235294117645 7 15 12.20561391223156 7 16 14.91050420168067 7 17 14.874019607843138 7 18 15.825233426704015 7 19 6.3956115779645195 7 20 14.421358543417368 7 21 13.425606909430439 7 22 14.3937558356676 7 23 9.929960317460315 7 24 2.27092670401494 7 25 12.088013538748832 7 26 9.213515406162466 7 27 11.639110644257702 7 28 8.838445378151258 7 29 6.946066760037349 7 30 8.082469654528477 7 31 12.663632119514473 7 32 7.8201213818860875 7 33 14.021825396825397 7 34 11.42952847805789 7 35 9.110037348272641 7 36 11.019432773109244 7 37 8.31162464985994 7 38 7.552836134453781 7 39 7.53921568627451 7 40 5.6466386554621835 8 1 -7.648225957049487 8 2 0.5223622782446284 8 3 -11.490627917833804 8 4 -11.540546218487393 8 5 -11.44623015873016 8 6 -11.581500933706817 8 7 -11.258204948646123 8 8 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17.452626050420168 9 11 5.586998132586368 9 12 6.465207749766574 9 13 7.610084033613445 9 14 0.8713235294117645 9 15 5.705613912231559 9 16 -0.9228291316526622 9 17 3.2073529411764703 9 18 -1.3414332399626527 9 19 3.5622782446311856 9 20 0.7546918767506998 9 21 -0.4077264239028935 9 22 4.5604225023342675 9 23 7.096626984126983 9 24 -1.3957399626517262 9 25 6.588013538748832 9 26 -1.7864845938375336 9 27 7.639110644257702 9 28 -1.4948879551820742 9 29 -1.0539332399626513 9 30 8.749136321195145 9 31 -1.8363678804855272 9 32 -1.5132119514472446 9 33 6.021825396825397 9 34 7.92952847805789 9 35 5.776704014939309 9 36 -0.6472338935574236 9 37 8.978291316526608 9 38 9.719502801120449 9 39 10.03921568627451 9 40 9.646638655462183 10 1 5.351774042950513 10 2 -9.477637721755372 10 3 9.509372082166196 10 4 -0.5405462184873926 10 5 11.55376984126984 10 6 14.418499066293183 10 7 8.741795051353877 10 8 8.978641456582633 10 9 14.202007469654529 10 10 18.952626050420168 10 11 10.586998132586368 10 12 10.465207749766574 10 13 19.610084033613447 10 14 -7.1286764705882355 10 15 -7.294386087768441 10 16 -5.422829131652662 10 17 -4.79264705882353 10 18 -4.341433239962653 10 19 -3.9377217553688144 10 20 -4.2453081232493 10 21 -4.4077264239028935 10 22 -3.9395774976657325 10 23 -4.403373015873017 10 24 -4.395739962651726 10 25 -4.911986461251168 10 26 -5.2864845938375336 10 27 -4.860889355742298 10 28 -4.994887955182074 10 29 -4.553933239962651 10 30 -4.250863678804855 10 31 -5.336367880485527 10 32 -5.013211951447245 10 33 -4.978174603174603 10 34 -5.07047152194211 10 35 -5.223295985060691 10 36 -5.147233893557424 10 37 -5.021708683473392 10 38 -5.2804971988795515 10 39 -4.96078431372549 10 40 -5.3533613445378165 11 1 3.351774042950513 11 2 2.5223622782446284 11 3 -9.490627917833804 11 4 -4.540546218487393 11 5 8.55376984126984 11 6 10.418499066293183 11 7 6.741795051353877 11 8 9.978641456582633 11 9 11.202007469654529 11 10 5.952626050420168 11 11 13.586998132586368 11 12 9.465207749766574 11 13 0.610084033613445 11 14 -2.1286764705882355 11 15 5.705613912231559 11 16 16.577170868347338 11 17 4.20735294117647 11 18 11.658566760037347 11 19 7.062278244631186 11 20 6.7546918767507 11 21 21.592273576097107 11 22 20.06042250233427 11 23 20.596626984126985 11 24 7.604260037348274 11 25 15.088013538748832 11 26 21.713515406162465 11 27 22.139110644257702 11 28 19.005112044817928 11 29 19.446066760037347 11 30 19.749136321195145 11 31 22.663632119514475 11 32 16.986788048552754 11 33 23.0218253968254 11 34 22.92952847805789 11 35 22.77670401493931 11 36 25.852766106442576 11 37 25.978291316526608 11 38 22.71950280112045 11 39 29.03921568627451 11 40 28.646638655462183 12 1 1.1017740429505132 12 2 3.0223622782446284 12 3 9.009372082166196 12 4 7.709453781512607 12 5 -8.44623015873016 12 6 9.418499066293183 12 7 6.991795051353877 12 8 8.228641456582633 12 9 7.952007469654529 12 10 9.202626050420168 12 11 5.836998132586368 12 12 5.465207749766574 12 13 2.110084033613445 12 14 11.871323529411764 12 15 5.455613912231559 12 16 6.827170868347338 12 17 0.7073529411764703 12 18 6.158566760037347 12 19 7.812278244631186 12 20 7.0046918767507 12 21 8.092273576097107 12 22 -0.4395774976657325 12 23 0.3466269841269831 12 24 6.354260037348274 12 25 3.5880135387488323 12 26 3.4635154061624664 12 27 -0.3608893557422981 12 28 4.255112044817926 12 29 2.4460667600373487 12 30 3.249136321195145 12 31 9.413632119514473 12 32 6.236788048552755 12 33 0.5218253968253972 12 34 9.67952847805789 12 35 10.026704014939309 12 36 5.602766106442576 12 37 8.728291316526608 12 38 3.4695028011204485 12 39 0.28921568627450966 12 40 7.8966386554621835 14 1 7.351774042950513 14 2 20.52236227824463 14 3 16.509372082166195 14 4 13.459453781512607 14 5 20.55376984126984 14 6 21.418499066293183 14 7 21.741795051353876 14 8 18.978641456582633 14 9 21.20200746965453 14 10 21.952626050420168 14 11 -8.413001867413632 14 12 -7.534792250233426 14 13 -7.389915966386555 14 14 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9.871323529411764 15 15 13.70561391223156 15 16 5.910504201680672 15 17 9.540686274509802 15 18 10.658566760037347 15 19 5.062278244631186 15 20 13.421358543417368 15 21 10.258940242763774 15 22 5.0604225023342675 15 23 6.929960317460317 15 24 6.27092670401494 15 25 6.421346872082166 15 26 22.713515406162465 15 27 15.80577731092437 15 28 16.338445378151256 15 29 9.112733426704017 15 30 12.082469654528477 15 31 17.663632119514475 15 32 18.986788048552754 15 33 14.35515873015873 15 34 23.92952847805789 15 35 24.110037348272645 15 36 22.18609943977591 15 37 22.31162464985994 15 38 17.71950280112045 15 39 25.03921568627451 15 40 21.646638655462183 16 1 -6.648225957049487 16 2 -0.47763772175537156 16 3 0.5093720821661964 16 4 -8.540546218487393 16 5 -1.4462301587301596 16 6 -8.081500933706817 16 7 -0.7582049486461226 16 8 1.9786414565826327 16 9 1.7020074696545286 16 10 3.452626050420168 16 11 -3.913001867413632 16 12 3.4652077497665736 16 13 4.610084033613445 16 14 -3.1286764705882355 16 15 3.2056139122315592 16 16 5.577170868347338 16 17 6.20735294117647 16 18 6.658566760037347 16 19 7.062278244631186 16 20 6.2546918767507 16 21 7.0922735760971065 16 22 6.0604225023342675 16 23 7.596626984126983 16 24 10.104260037348274 16 25 9.588013538748832 16 26 10.213515406162466 16 27 7.639110644257702 16 28 0.5051120448179258 16 29 8.446066760037349 16 30 7.749136321195145 16 31 7.663632119514473 16 32 5.986788048552755 16 33 -0.4781746031746028 16 34 -0.5704715219421104 16 35 7.776704014939309 16 36 0.35276610644257644 16 37 3.478291316526608 16 38 -0.7804971988795515 16 39 8.03921568627451 16 40 5.1466386554621835 17 1 3.101774042950513 17 2 -5.727637721755372 17 3 6.5093720821661964 17 4 0.7094537815126074 17 5 4.05376984126984 17 6 10.168499066293183 17 7 11.241795051353877 17 8 10.228641456582633 17 9 10.702007469654529 17 10 3.952626050420168 17 11 10.336998132586368 17 12 8.715207749766574 17 13 9.610084033613445 17 14 8.121323529411764 17 15 14.20561391223156 17 16 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-12.021358543417367 110 9 -11.797992530345471 110 10 -10.047373949579832 110 11 -8.413001867413632 110 12 -7.534792250233426 110 13 -7.389915966386555 110 14 -7.1286764705882355 110 15 -7.294386087768441 110 16 -5.422829131652662 110 17 -4.79264705882353 110 18 -4.341433239962653 110 19 -3.9377217553688144 110 20 -4.2453081232493 110 21 -4.4077264239028935 110 22 -3.9395774976657325 110 23 -4.403373015873017 110 24 -4.395739962651726 110 25 -4.911986461251168 110 26 -5.2864845938375336 110 27 -4.860889355742298 110 28 -4.994887955182074 110 29 -4.553933239962651 110 30 -4.250863678804855 110 31 -5.336367880485527 110 32 -5.013211951447245 110 33 -4.978174603174603 110 34 -5.07047152194211 110 35 -5.223295985060691 110 36 -5.147233893557424 110 37 -5.021708683473392 110 38 -5.2804971988795515 110 39 -4.96078431372549 110 40 -5.3533613445378165 111 1 12.351774042950513 111 2 14.022362278244628 111 3 16.009372082166195 111 4 14.959453781512607 111 5 19.55376984126984 111 6 11.418499066293183 111 7 17.241795051353876 111 8 14.478641456582633 111 9 16.20200746965453 111 10 4.452626050420168 111 11 -8.413001867413632 111 12 -7.534792250233426 111 13 -7.389915966386555 111 14 -7.1286764705882355 111 15 -7.294386087768441 111 16 -5.422829131652662 111 17 -4.79264705882353 111 18 -4.341433239962653 111 19 -3.9377217553688144 111 20 -4.2453081232493 111 21 -4.4077264239028935 111 22 -3.9395774976657325 111 23 -4.403373015873017 111 24 -4.395739962651726 111 25 -4.911986461251168 111 26 -5.2864845938375336 111 27 -4.860889355742298 111 28 -4.994887955182074 111 29 -4.553933239962651 111 30 -4.250863678804855 111 31 -5.336367880485527 111 32 -5.013211951447245 111 33 -4.978174603174603 111 34 -5.07047152194211 111 35 -5.223295985060691 111 36 -5.147233893557424 111 37 -5.021708683473392 111 38 -5.2804971988795515 111 39 -4.96078431372549 111 40 -5.3533613445378165 117 1 11.351774042950513 117 2 -12.477637721755372 117 3 -11.490627917833804 117 4 -11.540546218487393 117 5 -11.44623015873016 117 6 -11.581500933706817 117 7 -11.258204948646123 117 8 -12.021358543417367 117 9 -11.797992530345471 117 10 -10.047373949579832 117 11 -8.413001867413632 117 12 -7.534792250233426 117 13 -7.389915966386555 117 14 -7.1286764705882355 117 15 -7.294386087768441 117 16 -5.422829131652662 117 17 -4.79264705882353 117 18 -4.341433239962653 117 19 -3.9377217553688144 117 20 -4.2453081232493 117 21 -4.4077264239028935 117 22 -3.9395774976657325 117 23 -4.403373015873017 117 24 -4.395739962651726 117 25 -4.911986461251168 117 26 -5.2864845938375336 117 27 -4.860889355742298 117 28 -4.994887955182074 117 29 -4.553933239962651 117 30 -4.250863678804855 117 31 -5.336367880485527 117 32 -5.013211951447245 117 33 -4.978174603174603 117 34 -5.07047152194211 117 35 -5.223295985060691 117 36 -5.147233893557424 117 37 -5.021708683473392 117 38 -5.2804971988795515 117 39 -4.96078431372549 117 40 -5.3533613445378165 119 1 -18.648225957049487 119 2 -12.477637721755372 119 3 -11.490627917833804 119 4 -11.540546218487393 119 5 -11.44623015873016 119 6 -11.581500933706817 119 7 -11.258204948646123 119 8 -12.021358543417367 119 9 -11.797992530345471 119 10 -10.047373949579832 119 11 -8.413001867413632 119 12 -7.534792250233426 119 13 -7.389915966386555 119 14 -7.1286764705882355 119 15 -7.294386087768441 119 16 -5.422829131652662 119 17 -4.79264705882353 119 18 -4.341433239962653 119 19 -3.9377217553688144 119 20 -4.2453081232493 119 21 -4.4077264239028935 119 22 -3.9395774976657325 119 23 -4.403373015873017 119 24 -4.395739962651726 119 25 -4.911986461251168 119 26 -5.2864845938375336 119 27 -4.860889355742298 119 28 -4.994887955182074 119 29 -4.553933239962651 119 30 -4.250863678804855 119 31 -5.336367880485527 119 32 -5.013211951447245 119 33 -4.978174603174603 119 34 -5.07047152194211 119 35 -5.223295985060691 119 36 -5.147233893557424 119 37 -5.021708683473392 119 38 -5.2804971988795515 119 39 -4.96078431372549 119 40 -5.3533613445378165 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 631.0 5 27.0 6 41.0 7 57.0 8 62.0 9 121.0 10 108.0 11 98.0 12 54.0 13 56.0 14 39.0 15 31.0 16 22.0 17 10.0 18 20.0 19 20.0 20 30.0 21 52.0 22 73.0 23 72.0 24 83.0 25 70.0 26 52.0 27 46.0 28 26.0 29 9.0 30 8.0 31 3.0 32 1.0 33 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 100.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 0.0 100.0 6 0.0 0.0 0.0 100.0 7 92.1436004162331 3.1217481789802286 0.5723204994797086 4.162330905306972 8 92.66770670826833 4.264170566822672 0.62402496099844 2.4440977639105563 9 91.8622848200313 4.747000521648409 1.1476264997391759 2.2430881585811164 10 52.05836373110996 27.20166753517457 9.640437727983326 11.099531005732151 11 37.20083246618106 25.962539021852237 21.644120707596255 15.192507804370447 12 32.65730629225169 22.100884035361414 23.608944357774313 21.632865314612584 13 22.384174908901613 24.67464862051015 25.143154606975532 27.798021863612703 14 17.741935483870968 29.916753381893862 31.113423517169615 21.22788761706556 15 25.221008840353615 30.681227249089964 27.66510660426417 16.43265730629225 16 30.577223088923557 29.95319812792512 22.152886115444616 17.31669266770671 17 25.377015080603226 31.3052522100884 28.029121164846593 15.288611544461778 18 25.949037961518464 28.23712948517941 27.821112844513777 17.99271970878835 19 25.962539021852237 30.697190426638915 23.621227887617067 19.719042663891777 20 25.117004680187204 31.56526261050442 27.301092043681745 16.016640665626625 21 23.660946437857515 28.861154446177846 26.72906916276651 20.74882995319813 22 20.852834113364533 27.301092043681745 28.39313572542902 23.452938117524702 23 21.684867394695786 30.31721268850754 27.405096203848156 20.59282371294852 24 20.64482579303172 31.201248049921997 26.521060842433698 21.632865314612584 25 22.67290691627665 34.321372854914195 23.816952678107125 19.18876755070203 26 21.852237252861602 32.25806451612903 24.97398543184183 20.915712799167537 27 17.760416666666668 30.9375 25.260416666666668 26.041666666666668 28 20.114643043251693 32.72537780093799 25.325690463783218 21.834288692027098 29 24.413145539906104 32.34220135628586 24.152321335419927 19.092331768388107 30 22.724908996359854 28.91315652626105 30.213208528341134 18.14872594903796 31 25.637025481019244 31.461258450338015 25.949037961518464 16.952678107124285 32 24.137931034482758 27.638453500522463 24.76489028213166 23.458725182863112 33 23.608944357774313 30.00520020800832 26.781071242849713 19.604784191367656 34 21.42485699427977 30.78523140925637 29.6411856474259 18.14872594903796 35 19.34477379095164 33.229329173166924 27.92511700468019 19.50078003120125 36 19.238790406673616 33.5245046923879 27.059436913451513 20.177267987486967 37 20.95683827353094 31.357254290171603 29.6411856474259 18.044721788871556 38 21.436004162330903 30.385015608740893 27.99167533818939 20.187304890738815 39 22.516900676027042 31.201248049921997 23.712948517940717 22.568902756110244 40 20.904836193447736 29.17316692667707 25.533021320852832 24.38897555902236 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 2.5 10 5.0 11 3.5 12 2.0 13 2.0 14 10.5 15 19.0 16 18.0 17 17.0 18 17.0 19 16.0 20 15.0 21 14.0 22 13.0 23 13.0 24 14.5 25 16.0 26 14.5 27 13.0 28 13.0 29 12.5 30 12.0 31 21.0 32 30.0 33 30.0 34 39.5 35 49.0 36 49.0 37 49.0 38 49.0 39 68.0 40 87.0 41 82.0 42 77.0 43 77.0 44 103.0 45 129.0 46 139.5 47 150.0 48 150.0 49 156.5 50 163.0 51 176.0 52 189.0 53 189.0 54 190.0 55 191.0 56 172.0 57 153.0 58 153.0 59 151.0 60 149.0 61 147.5 62 146.0 63 146.0 64 137.5 65 129.0 66 95.5 67 62.0 68 62.0 69 48.0 70 34.0 71 22.5 72 11.0 73 11.0 74 8.5 75 6.0 76 5.0 77 4.0 78 4.0 79 2.5 80 1.0 81 1.5 82 2.0 83 2.0 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.05200208008320333 8 0.0 9 0.31201248049922 10 0.20800832033281333 11 0.05200208008320333 12 0.0 13 0.10400416016640666 14 0.05200208008320333 15 0.0 16 0.0 17 0.0 18 0.0 19 0.05200208008320333 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.05200208008320333 27 0.15600624024961 28 0.20800832033281333 29 0.31201248049922 30 0.0 31 0.0 32 0.46801872074883 33 0.0 34 0.0 35 0.0 36 0.26001040041601664 37 0.0 38 0.05200208008320333 39 0.0 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 1923.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.88767550702028 #Duplication Level Percentage of deduplicated Percentage of total 1 95.41595925297113 87.6755070202808 2 3.395585738539898 6.240249609984399 3 0.5659309564233164 1.5600624024960998 4 0.2829654782116582 1.0400416016640666 5 0.11318619128466327 0.5200208008320333 6 0.11318619128466327 0.62402496099844 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.11318619128466327 2.3400936037441498 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ACACCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT 23 1.1960478419136766 No Hit ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC 22 1.1440457618304731 No Hit ACACCCGGGGGGGAGGTAGTGACGAAAAATAACAATACAG 6 0.31201248049922 No Hit ACACCCCAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6 0.31201248049922 No Hit ACACCCGGGATGCCACAACTAGATACATCAACATGATTTA 5 0.26001040041601664 No Hit ACACCCGGGACTAATCCTAGCCCTAGCCCTACACAAATAT 5 0.26001040041601664 No Hit ACACCCCAAAAAAAAAAAAAACAAAAAAAAAAAAAAAAAA 4 0.20800832033281333 No Hit ACACCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG 4 0.20800832033281333 No Hit ACACCCGGGATGCCATCTACCTTCTTCAACCTCACCATAG 4 0.20800832033281333 No Hit ACACCCGGGATATTATTTGATCTTATCTTGGATCCTTTTG 4 0.20800832033281333 No Hit ACACCCACCAAAACAAAAAAAAAAAAAAAAAAAAAAAAAA 4 0.20800832033281333 No Hit ACACCCCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3 0.15600624024961 No Hit ACACCCGGGGAGTCCCCATGATACTCCCGGGTGTTCTGAG 3 0.15600624024961 No Hit ACACCCGGGATCGAAATTCCTACCGGTGTCAAAGTATTTA 3 0.15600624024961 No Hit ACACCCGGGGATCTCCAGCTATACTATGAGCCTTAGGCTT 3 0.15600624024961 No Hit ACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3 0.15600624024961 No Hit ACACCCGGGATGGCCTACCCATTCCAACTTGGTCTACAAG 3 0.15600624024961 No Hit ACACCCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGC 3 0.15600624024961 No Hit ACACCCGGGGGGAGTAGTTGTGGAAAGCAGGCCTCTGTAC 3 0.15600624024961 No Hit ACACCCGGGGTGACGACTCAGTGATCCAGCTCCTGAACCC 3 0.15600624024961 No Hit ACACCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA 3 0.15600624024961 No Hit ACACCCGGGGTGACCATAACCTTTGCTGTGCTGAGTAAAG 2 0.10400416016640666 No Hit ACACCCGGGCCTTTAAAATTATTTAAATCTAGATTTCAGG 2 0.10400416016640666 No Hit ACACCCGGGGGGTTTTTGTTTGTTAGTTTGTTTCTTGTCC 2 0.10400416016640666 No Hit ACACCCGGGAATCTTCTTAGTCAAATGCTGGAAGCCCCAG 2 0.10400416016640666 No Hit ACACCCGGGATGCCACAAATAGATACATCAACATGATTTA 2 0.10400416016640666 No Hit ACACCCGGGACGCACGGCCGGTACAGTGAAACTGCGAATG 2 0.10400416016640666 No Hit ACACCCGGGAGATGAAGTTTTTGTTACTTGTCTCTAAAGT 2 0.10400416016640666 No Hit ACACCCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT 2 0.10400416016640666 No Hit ACACCCGGGTACCTGGTTGATACTGCCAGTAGCATATGCT 2 0.10400416016640666 No Hit ACACCCGGGGATAAGGTGCTCTTCTTTTCCTTCCCCTCAA 2 0.10400416016640666 No Hit ACACCCGGGAACTCATAACTATACACTGTAATGCAAGCAT 2 0.10400416016640666 No Hit ACACCCGGGGGACTTTTGAAGTGTTGGAATTTTTGAAGAC 2 0.10400416016640666 No Hit ACACCCGGGGTGTGTGTGTGTGTGTGTGAGAGAGAGAGAG 2 0.10400416016640666 No Hit ACACCCGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACT 2 0.10400416016640666 No Hit ACACCCGGGATTCGTAGGAGTAAACATAACATTCTTCCCT 2 0.10400416016640666 No Hit ACACCCGGGGCCTCTTCGAGATCATTGGGGTCAAGAGCCA 2 0.10400416016640666 No Hit ACACCCCAACAAAAAAAAAAAAAAAAAAAAAATAAAAAAA 2 0.10400416016640666 No Hit ACACCCAAACAAACAAAAAAAAAAAAAAAAAAAAAAAAAA 2 0.10400416016640666 No Hit ACACCCGGGGAAGTCTCTTAACCCAGAACTGGGCACTGAC 2 0.10400416016640666 No Hit ACACCCGGGGATTGCTTGCTCCTGGAAACTATAAACTTAC 2 0.10400416016640666 No Hit ACACCCGGGGTGACATTGCAACTCCAATCAGTTAGTAAGC 2 0.10400416016640666 No Hit ACACCCGGGGGATTAGATGTAGACACACGAGCTTACTTTA 2 0.10400416016640666 No Hit ACACCCGGGGAACTTGCTCACCTTCAATGTGGGCTATCAT 2 0.10400416016640666 No Hit ACACCCGGGGTCGGGTTCTATTTGGGAGTGACTGCGTTTT 2 0.10400416016640666 No Hit ACACCCGGGGTTCACTGATTCCCATTATTTTCAGGCTTCA 2 0.10400416016640666 No Hit ACACCCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGA 2 0.10400416016640666 No Hit ACACCCGGGGTGTTCTTTATTAATATCCTAACACTCCTCG 2 0.10400416016640666 No Hit ACACCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAG 2 0.10400416016640666 No Hit ACACCCGGGTCTCCCTATTCGATAAAGATGGTGACGGCAC 2 0.10400416016640666 No Hit ACACCCGGGAGGAAGGGAGGTTTGCCTGTTGTGCTTGCAA 2 0.10400416016640666 No Hit ACACCCGGGGGTTCGATTCCGGAGAGGGAGCCTGAGAAAC 2 0.10400416016640666 No Hit ACACCCGGGAGGGATGTTCCCGGCTCAGGAGGAGGCTGAC 2 0.10400416016640666 No Hit ACACCCGGGGGAGCTGGAGAAATGGCTCAGTGCTTAAGAG 2 0.10400416016640666 No Hit ACACCCGGGGTTGTTTTGGATTCCTTTGGGTTTTGCTTTG 2 0.10400416016640666 No Hit ACACCCGGGGTTTTACGTTTTTGTTTTTTGGCTCCTGGAT 2 0.10400416016640666 No Hit ACACCCGGGGGTCTCTGCATCTGTGACTAACACAGAAACA 2 0.10400416016640666 No Hit ACACCCGGGGCTCTTTTCCTCCTCTCCAGCTCCGGCGCCG 2 0.10400416016640666 No Hit ACACCCGGGACCCTTTGGAGCTGCAGCTGTGCCCGGCACA 2 0.10400416016640666 No Hit ACACCCGGGATCAATATTTTCACAACCTCAATCTTATTAA 2 0.10400416016640666 No Hit ACACCCGGGGGGGCTGGTGAGCTGGCTCAGTGGGTAAGAG 2 0.10400416016640666 No Hit ACACCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA 2 0.10400416016640666 No Hit ACACCCGGGACTAATCCTAGCACTAGCCCTACACAAATAT 2 0.10400416016640666 No Hit ACACCCGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT 2 0.10400416016640666 No Hit ACACCCGGGGATCGTTTCAAGGGTGGGATTCATCCCTCAG 2 0.10400416016640666 No Hit ACACCCGGGGTAGCCCATTTCCACTATGTTCTATCAATGG 2 0.10400416016640666 No Hit ACACCCGGGGGGCTGGTGAGATGGCTCAGCGGTTAAGAGC 2 0.10400416016640666 No Hit ACACCCGGGAGCCCACAGAGCTAATGGTACCTCAATCTAA 2 0.10400416016640666 No Hit ACACCCGGGGTGTTTGCTATCATAGCAGGATTTGTTCACT 2 0.10400416016640666 No Hit ACACCCGGGGGGTGAGTTCTGCTACTGCAGTGTGTCCATA 2 0.10400416016640666 No Hit ACACCCGGGCCTGTGAAGTCTGACAACAAGGATGATGACA 2 0.10400416016640666 No Hit ACACCCGGGGGCTTTCTGAAGATGGCGGCGGCTAAGGACG 2 0.10400416016640666 No Hit ACACCCGGGAGGGTTCGATTCCGGAGAGGGAGCCTGAGAA 2 0.10400416016640666 No Hit ACACCCGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT 2 0.10400416016640666 No Hit ACACCCGGGAACTTAATTATGTCTCACTATGGTTTCTACA 2 0.10400416016640666 No Hit ACACCCGGGGGCTACCACATCCAAGGAAGGCAGCAGGCGC 2 0.10400416016640666 No Hit ACACCCGGGGGAAAAAACCTTTAATAGTGAATAATTAACA 2 0.10400416016640666 No Hit ACACCCGGGATTCTTAGGGAAGAGAGCTTTGAACGAAAAG 2 0.10400416016640666 No Hit ACACCCGGGGCAGGATTTGTTCACTGATTCCCATTATTTT 2 0.10400416016640666 No Hit ACACCCGGGGGAGAGGGAGCCTGAGAAACGGCTACCACAT 2 0.10400416016640666 No Hit ACACCCGGGGAGTCGACTTTTTTTTTTGTTTGTTTTTTTT 2 0.10400416016640666 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGGGG 20 0.0021482338 34.000004 6 CCCGGGT 20 0.0021482338 34.000004 4 CCGGGGT 20 0.0021482338 34.000004 5 CCGGGGG 35 5.147558E-7 34.000004 5 CCGGGAG 20 0.0021482338 34.000004 5 CCCGGGA 55 5.456968E-12 34.0 4 ACACCCG 170 0.0 34.0 1 ACCCGGG 170 0.0 34.0 3 CACCCGG 170 0.0 34.0 2 CCCGGGG 85 0.0 32.0 4 >>END_MODULE