FastQCFastQC Report
Fri 17 Jun 2016
SRR1526026_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526026_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148172
Sequences flagged as poor quality0
Sequence length56
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG54963.7092028183462458No Hit
CGGCAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG14190.9576708149987851No Hit
CGGCAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG7040.4751235051156764No Hit
CGGCAGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC6460.4359798072510326No Hit
CGGCAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG6250.4218070890586616No Hit
CGGCAGGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT5790.3907620873039441No Hit
CGGCAGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT5660.38198849985152394No Hit
CGGCAGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC5070.3421699106443863No Hit
CGGCAGGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC4390.2962772993548039No Hit
CGGCAGGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA3890.262532732230111No Hit
CGGCAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG3880.2618578408876171No Hit
CGGCAGGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC3480.23486218718786275No Hit
CGGCAGGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC3380.22811327376292415No Hit
CGGCAGGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG2230.15050076937613044No Hit
CGGCAGGGGATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT2220.1498258780336366No Hit
CGGCAGGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT2130.14375185595119186No Hit
CGGCAGGGGGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGT2090.14105229058121643No Hit
CGGCAGGGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCC2060.13902761655373486No Hit
CGGCAGGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT2040.13767783386874713No Hit
CGGCAGGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC2010.13565315984126555No Hit
CGGCAGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGACCTAATACATGCCGAC2000.1349782684987717No Hit
CGGCAGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGCGCTAATACATGCCGAC1980.13362848581378398No Hit
CGGCAGGGGACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTT1950.1316038117863024No Hit
CGGCAGGGGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCG1930.1302540291013147No Hit
CGGCAGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC1910.12890424641632697No Hit
CGGCAGGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG1870.12620468104635152No Hit
CGGCAGGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG1830.1235051156763761No Hit
CGGCAGGGGAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGA1800.12148044164889452No Hit
CGGCAGGGGAACTGTGGTAATTCTAGAGCTAATACATGCCCACGGGCGCTGACCCC1680.11338174553896822No Hit
CGGCAGGGGAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCA1660.11203196285398051No Hit
CGGCAGGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAG1580.10663283211402964No Hit
CGGCAGGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG1530.10325837540156035No Hit
CGGCAGGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACCACCCATTCGAACGTC1530.10325837540156035No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1490.10055881003158491No Hit
CGGCAGGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGG1490.10055881003158491No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAATA402.41198E-950.5469841
CGCGAAT150.00806207650.5469841
CTTATGG150.00806207650.5469841
GATTGAC203.953744E-450.5469841
ACCTAAT402.4338078E-950.49503340
ACTTATG150.00809492650.4950340
AACCGCG150.00810589950.47773738
TATGCCT800.050.3914535
CGGGATC150.00816092750.3914535
TTAGTTC204.0347327E-450.3398234
CCACGAT600.050.20265646
TTGACCA150.0083276850.1343543
CGGCTCT204.1170343E-450.1343544
TCGACAC150.0083276850.1343543
ACTTTAT204.151718E-450.04923614
AGATTAC204.1796314E-449.9813549
GATTACG204.1866332E-449.9644150
GTGCCTT150.00846289749.93055312
GACGCAC252.1041351E-549.896759
AACATGA150.0084855949.89674830
Produced by FastQC (version 0.11.5)