FastQCFastQC Report
Fri 17 Jun 2016
SRR1526025_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526025_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12297
Sequences flagged as poor quality0
Sequence length40
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT1050.8538667967797022No Hit
CGGCAGGGGTACCTGGTTGATCCTGCCCGTAGCATATGCT510.4147353012929983No Hit
CGGCAGGGGTACCTGGTTGATCCTGCCCGTAGCCTATGCT430.3496787834431162No Hit
CGGCAGGGGTACCTGGTTGATCCTGCCAGTAGCCTATGCT400.32528258924941045No Hit
CGGCAGGGGATGCCACAACTAGATACATCAACATGATTTA280.2276978124745873No Hit
CGGCAGGGGTGAGATGGACTTAAAGGAATTGACGGGTACG230.187037488818411No Hit
CGGCAGGGGTACCTGGTTCATCCTGCCAGTAGCATATGCT170.1382451004309994No Hit
CGGCAGGGGTGAGATGGACTTAAATGAATTGACGGGTACG140.11384890623729366No Hit
CGGCAGGGGTACCTGGTTGATCCTGCCAGTAGCATCTGCT140.11384890623729366No Hit
CGGCAGGGGTACCTGGTTGATCCTGCCAGTAGCCTCTGCT140.11384890623729366No Hit
CGGCAGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT130.10571684150605838No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGAATT301.3417317E-534.09426524
TTGACGG301.3417317E-534.09426529
TGAATTG301.3417317E-534.09426525
GGGTACG200.002646558234.0942634
GACGGGT251.8824515E-434.0942631
TGCCCGT251.8824515E-434.0942624
CGGGTAC251.8824515E-434.0942633
ACGGGTA251.8824515E-434.0942632
ATTGACG251.8824515E-434.0942628
TGACGGG251.8824515E-434.0942630
AGGGGTT301.3803248E-533.95515
AGGGGTC200.002700615433.95515
AGGGGTA700.033.95515
GATGGAC407.097515E-833.955113
AGGGGGG1750.033.95515
AGGGGGC650.033.95515
AGGGGGA750.033.95515
AGGGGCT552.5465852E-1133.95515
AGGGGCC359.893902E-733.95515
AGGGGCA359.893902E-733.95515