FastQCFastQC Report
Fri 17 Jun 2016
SRR1526023_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526023_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6642
Sequences flagged as poor quality0
Sequence length40
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT3555.3447756699789215No Hit
CGGCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.4817825956037338No Hit
CGGCAGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT280.4215597711532671No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC260.3914483589280337No Hit
CGGCAGGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT180.27100271002710025No Hit
CGGCAGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG180.27100271002710025No Hit
CGGCAGGGGACTAATCCTAGCCCTAGCCCTACACAAATAT170.25594700391448355No Hit
CGGCAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGAT140.21077988557663355No Hit
CGGCAGGGGTACATGGTTGATCCTGCCAGTAGCATATGCT140.21077988557663355No Hit
CGGCAGGGGTACCTGGTTGATACTGCCAGTAGCATATGCT130.19572417946401685No Hit
CGGCAGGGGTCCCTGGTTGATCCTGCCAGTAGCATATGCT130.19572417946401685No Hit
CGGCAGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGA110.1656127672387835No Hit
CGGCAGGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT110.1656127672387835No Hit
CGGCAGGGGTACCTGGTTGACCCTGCCAGTAGCATATGCT100.15055706112616682No Hit
CGGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG90.13550135501355012No Hit
CGGCAGGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCT80.12044564890093346No Hit
CGGCAGGGGGGAGAGGGAGCCTGAGAAACGGCTACCACAT80.12044564890093346No Hit
CGGCAGGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACT80.12044564890093346No Hit
CGGCAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCC70.10538994278831677No Hit
CGGCAGGGGCGGGGAGGTAGTGACGAAAAATAACAATACA70.10538994278831677No Hit
CGGCAGGGGATTTACAGTTGGTGGTCTAACCGGAATTGTT70.10538994278831677No Hit
CGGCAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.10538994278831677No Hit
CGGCAGGGGAGCATATGCTTGTCTCAAAGATTAAGCCATG70.10538994278831677No Hit
CGGCAGGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG70.10538994278831677No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGATC750.034.2061116
TGGTTGA750.034.2061114
GGTTGAT750.034.2061115
ACCTGGT601.8189894E-1234.2061111
TTGATCC750.034.2061117
CCTGGTT601.8189894E-1234.2061112
CTGGTTG700.034.20610413
AGGGGTA1000.033.946975
ATCCTGC850.033.9469720
CCTGCCA850.033.9469722
CAGGGGT1300.033.946974
CAGGGGC650.033.946974
TCCTGCC850.033.9469721
AGGGGTC200.002613745233.9469685
AGGGGGG1200.033.9469685
AGGGGGA750.033.9469685
AGGGGCT301.2871218E-533.9469685
AGGGGCA200.002613745233.9469685
AGGGGAT601.8189894E-1233.9469685
AGGGGAG406.285882E-833.9469685
Produced by FastQC (version 0.11.5)