FastQCFastQC Report
Fri 17 Jun 2016
SRR1526022_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526022_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64820
Sequences flagged as poor quality0
Sequence length56
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG11621.7926565874730023No Hit
ACTTATGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT5670.8747300215982721No Hit
ACTTATGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC5230.8068497377352669No Hit
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG3250.5013884603517432No Hit
ACTTATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1860.28694847269361307No Hit
ACTTATGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA1840.2838630052452946No Hit
ACTTATGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC1630.25146559703795124No Hit
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG1430.22061092255476702No Hit
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG1300.20055538414069735No Hit
ACTTATGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT1180.1820425794507868No Hit
ACTTATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA970.1496451712434434No Hit
ACTTATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA960.14810243751928417No Hit
ACTTATGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC870.13421783400185128No Hit
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG830.12804689910521444No Hit
ACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC800.12341869793273681No Hit
ACTTATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT780.12033323048441838No Hit
ACTTATGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC660.10182042579450787No Hit
ACTTATGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT660.10182042579450787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTCA309.3559356E-750.71732341
CCCCGAC150.0079350250.71732341
GCATTGA203.91735E-450.597840
TCGTCTC251.9249166E-550.597840
TGCTCGT251.9249166E-550.597837
TGCCCCA150.00800945950.59779738
TTGTCCA150.00800945950.59779737
AGACCGC150.00800945950.59779737
CTACCAC150.00800945950.59779738
CGTCGAG150.00800945950.59779738
TCGAGCA150.00800945950.59779740
GCTACCA150.00800945950.59779737
GCGTCGA150.00800945950.59779737
CGCATTG203.9325817E-450.55808639
GTCGAGC150.00803438750.55808339
ATGCTCG251.9519608E-550.4788436
ACGGCTA150.00815989350.36043534
GTCATCA150.00821050250.2818146
TTGGATT150.00821050250.2818144
TCCACGA251.9977337E-550.2818145
Produced by FastQC (version 0.11.5)