##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526022_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 64820 Sequences flagged as poor quality 0 Sequence length 56 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.65097192224622 39.0 36.0 40.0 33.0 40.0 2 35.82526997840173 39.0 36.0 40.0 27.0 40.0 3 36.097269361308236 39.0 36.0 40.0 29.0 40.0 4 36.01908361616785 39.0 37.0 40.0 28.0 40.0 5 35.31172477630361 38.0 35.0 39.0 27.0 40.0 6 35.48829065103363 39.0 36.0 40.0 26.0 40.0 7 36.02878741129281 39.0 37.0 40.0 29.0 40.0 8 35.88589941376119 39.0 36.0 40.0 29.0 40.0 9 35.87107374267202 39.0 36.0 40.0 29.0 40.0 10 35.260290033940144 39.0 35.0 40.0 26.0 40.0 11 34.966352977476085 38.0 35.0 40.0 26.0 40.0 12 33.887642702869485 38.0 33.0 39.0 21.0 40.0 13 33.235606294353595 38.0 32.0 39.0 18.0 40.0 14 33.921397716754086 38.0 33.0 39.0 21.0 40.0 15 32.61066029003394 38.0 31.0 39.0 15.0 40.0 16 32.233014501697006 37.0 29.0 39.0 17.0 40.0 17 31.067294045047824 36.0 27.0 39.0 13.0 40.0 18 30.464270286948473 35.0 25.0 39.0 12.0 40.0 19 31.706325208269053 36.0 28.0 39.0 16.0 40.0 20 32.460259179265655 37.0 30.0 39.0 19.0 40.0 21 32.669345880900956 38.0 30.0 39.0 17.0 40.0 22 31.918836778771983 37.0 29.0 39.0 14.0 40.0 23 32.73556001234187 38.0 31.0 39.0 17.0 40.0 24 32.032196852823205 37.0 29.0 39.0 15.0 40.0 25 32.543582227707496 38.0 30.0 39.0 17.0 40.0 26 33.014856525763655 38.0 32.0 39.0 17.0 40.0 27 33.2362388151805 38.0 33.0 39.0 18.0 40.0 28 33.136192533168774 38.0 32.0 39.0 18.0 40.0 29 32.7207343412527 38.0 31.0 39.0 16.0 40.0 30 32.48849120641777 37.0 31.0 39.0 15.0 40.0 31 33.23366244986116 38.0 33.0 39.0 17.0 40.0 32 33.007528540573894 38.0 33.0 39.0 16.0 40.0 33 32.82235421166307 38.0 32.0 39.0 15.0 40.0 34 32.67344955260722 38.0 32.0 39.0 12.0 40.0 35 32.53940141931503 38.0 32.0 39.0 10.0 40.0 36 31.614779389077444 36.0 30.0 39.0 4.0 40.0 37 31.387133600740512 36.0 30.0 39.0 4.0 40.0 38 30.74745448935514 36.0 29.0 38.0 4.0 40.0 39 29.88205800678803 35.0 27.0 38.0 4.0 40.0 40 27.720996605985807 34.0 18.0 38.0 4.0 40.0 41 25.599537179882752 31.0 12.0 38.0 4.0 39.0 42 26.514763961740204 32.0 18.0 38.0 4.0 39.0 43 26.548503548287567 32.0 17.0 38.0 4.0 39.0 44 25.642486886763344 31.0 13.0 37.0 4.0 39.0 45 26.71152422091947 32.0 18.0 38.0 4.0 39.0 46 24.703841406973158 29.0 10.0 36.0 4.0 39.0 47 23.6787102746066 28.0 6.0 36.0 4.0 39.0 48 24.407343412527 29.0 8.0 36.0 4.0 39.0 49 23.35735883986424 28.0 4.0 36.0 4.0 39.0 50 24.02528540573897 29.0 4.0 36.0 4.0 39.0 51 24.33508176488738 30.0 4.0 36.0 4.0 39.0 52 24.289231718605368 30.0 4.0 36.0 4.0 39.0 53 24.08557543967911 30.0 4.0 36.0 4.0 39.0 54 23.72190681888306 30.0 4.0 36.0 4.0 39.0 55 23.511786485652575 30.0 4.0 36.0 4.0 39.0 56 22.87062634989201 29.0 4.0 36.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 3.565903260822921 1 2 4.762317638048081 1 3 4.664655854445748 1 4 4.69705071795422 1 5 5.239806719559148 1 6 5.082362831671958 1 7 4.809097647523664 1 8 4.871379558308803 1 9 4.914603016224611 1 10 5.2621785029405 1 11 1.490383860755614 1 12 -4.989606753134616 1 13 -17.886161537846824 1 14 -4.986803481081882 1 15 1.809881086514043 1 16 -0.890608754169385 1 17 -4.973198937322305 1 18 -3.385473751993093 1 19 -0.25533734287839494 1 20 -0.3067410912862343 1 21 7.237634342176779 1 22 8.047875318610231 1 23 7.209107258315875 1 24 7.533042576296214 1 25 6.948383850910702 1 26 7.10122410422079 1 27 6.546572352188967 1 28 6.771925923148633 1 29 6.8360632882586785 1 30 7.206039172286786 1 31 6.27955215095513 1 32 6.274346282702901 1 33 6.47119615357709 1 34 6.4596566330271905 1 35 6.533511155552262 1 36 6.0213937599045195 1 37 6.065586677000411 1 38 6.580437724459724 1 39 6.882915418512535 1 40 -6.840023653635626 1 41 2.4587338592572507 1 42 2.061221178148738 1 43 -4.946766674646771 1 44 -12.380518887271991 1 45 1.51513146471763 1 46 -2.6029815642131986 1 47 -7.5692964000662 1 48 -2.966689176944712 1 49 -12.116012840689402 1 50 -1.2361786495765728 1 51 5.892582378529937 1 52 5.686265884109936 1 53 5.7663663178238735 1 54 5.681012130781731 1 55 5.980100542639416 1 56 8.45256886697008 2 1 3.6703099719609824 2 2 4.885826835541515 2 3 4.585011625620197 2 4 4.799941358746622 2 5 4.6464167862662435 2 6 5.2939051805702775 2 7 4.639196697452917 2 8 4.792746043047032 2 9 4.571768975391784 2 10 5.383464130593623 2 11 4.422160695200731 2 12 1.5531464306131078 2 13 2.3656072108897845 2 14 5.31711808754557 2 15 6.080550178893258 2 16 5.3590374960832925 2 17 7.849218689522246 2 18 6.371348564562396 2 19 6.957620005009215 2 20 6.219234247302808 2 21 7.663650109308374 2 22 8.302474065325413 2 23 7.39881819423664 2 24 8.028797579328359 2 25 6.85640891660708 2 26 6.718466877618809 2 27 6.64804799399208 2 28 5.903520829152274 2 29 6.736002645444849 2 30 7.304785887467702 2 31 5.665846875030322 2 32 5.603636761781132 2 33 5.323631973265783 2 34 4.8666709851597965 2 35 5.2927591846608095 2 36 6.925881328127687 2 37 6.920852494667685 2 38 7.134106614696233 2 39 7.473677496539626 2 40 6.555872849295444 2 41 9.681899414138996 2 42 8.849173472468525 2 43 7.426894129881223 2 44 -0.8944667344521022 2 45 9.048485012322239 2 46 6.747634971060705 2 47 3.7828362055533695 2 48 6.157830734112494 2 49 -0.6970720685042409 2 50 7.45464407126434 2 51 10.81283707834801 2 52 9.861220401999567 2 53 10.280718450429493 2 54 10.532841522332166 2 55 10.51365623295678 2 56 11.461766360400443 3 1 3.7618715966108454 3 2 4.8586909811997785 3 3 4.857770729261659 3 4 4.8710197901191705 3 5 5.0471520803838885 3 6 5.46389817776916 3 7 4.791332551794653 3 8 4.8797558469685995 3 9 4.995788583234919 3 10 5.17390530706421 3 11 5.053988426293174 3 12 5.948629623890422 3 13 6.524920936379978 3 14 5.664982233203833 3 15 2.5226019996215534 3 16 6.880746179556684 3 17 8.568406364592278 3 18 9.182272934310298 3 19 7.987206839743106 3 20 6.987441530215975 3 21 7.437284563089886 3 22 9.020261180171353 3 23 7.995281779670812 3 24 8.316087495294742 3 25 7.688166619688317 3 26 6.510833824397523 3 27 5.9041754449724735 3 28 6.524319148480007 3 29 6.839469031999471 3 30 6.931186447691793 3 31 5.888535950660579 3 32 6.575800627327347 3 33 6.130529732369425 3 34 6.196677987960914 3 35 6.982885235080978 3 36 8.242583008799954 3 37 8.223723643127066 3 38 9.15564022814161 3 39 8.9076760959794 3 40 10.77733643472962 3 41 13.675596893130596 3 42 12.571162267986733 3 43 12.100913737724362 3 44 12.884945030253782 3 45 11.472329550137367 3 46 12.104952898231577 3 47 12.99992304028726 3 48 12.668159865765151 3 49 13.519664626173633 3 50 12.58874771272092 3 51 12.612206826247167 3 52 11.574980906201244 3 53 11.877006965835658 3 54 11.820831718410599 3 55 12.312850910827926 3 56 11.674126304378035 4 1 3.65090220885574 4 2 5.183946083240592 4 3 4.941954402731042 4 4 5.138876932976316 4 5 5.122407182424702 4 6 5.546806341034461 4 7 5.004342755876287 4 8 4.8427660510502335 4 9 5.01874776690839 4 10 5.073140000941763 4 11 5.598631283436028 4 12 6.6029663585843 4 13 6.9866556302575304 4 14 5.941767947489549 4 15 7.051938734315431 4 16 8.610338016291376 4 17 9.165345140102481 4 18 10.529211709820501 4 19 8.970625207090048 4 20 8.155808877154747 4 21 7.846723338600089 4 22 9.055975465885638 4 23 7.522067493956527 4 24 7.799505862641684 4 25 8.194544170708724 4 26 7.411344028479149 4 27 7.012593812319409 4 28 6.287074250520821 4 29 7.2642139299586574 4 30 7.020472161977509 4 31 6.43955635882384 4 32 5.9061577701844925 4 33 5.603744018083717 4 34 5.307647375716019 4 35 6.4140076840605715 4 36 7.266817702677507 4 37 7.221172622718903 4 38 8.156915738345692 4 39 7.893645483734506 4 40 3.832183373505128 4 41 12.374576484967328 4 42 11.94361124757857 4 43 11.06775047241824 4 44 12.094128703723172 4 45 10.134319346055733 4 46 13.506738612517285 4 47 12.867269979062772 4 48 13.516374151479443 4 49 13.328338095561389 4 50 13.070890569863781 4 51 12.778023152777784 4 52 12.318603355180837 4 53 13.073435537264228 4 54 12.288943963308558 4 55 11.699330502664665 4 56 10.621830386010686 5 1 3.844779759876147 5 2 4.755374654669168 5 3 4.758280933343286 5 4 4.873570810527333 5 5 5.153019427322661 5 6 5.4447655247079325 5 7 4.504342755876287 5 8 4.720317071458396 5 9 4.7840538893573665 5 10 5.175180817268291 5 11 5.445570058946231 5 12 6.358068399400626 5 13 6.537676038420791 5 14 6.196869988305874 5 15 7.480510162886855 5 16 7.9878890366995385 5 17 9.420447180918806 5 18 9.67206885267764 5 19 8.480829288722699 5 20 7.798666020011893 5 21 7.897743746763357 5 22 8.831485669967272 5 23 7.787373616405503 5 24 8.197465046315155 5 25 8.000666619688317 5 26 6.952160355009767 5 27 6.992185649054107 5 28 6.715645679092251 5 29 7.60094862383621 5 30 7.061288488508119 5 31 5.93955635882384 5 32 6.4061577701844925 5 33 6.399662385430652 5 34 6.205606559389491 5 35 6.577272990183019 5 36 7.828042192473426 5 37 8.159948132922985 5 38 8.21814022814161 5 39 8.607931198020221 5 40 10.781162965341863 5 41 12.456209138028555 5 42 11.025243900639797 5 43 11.02693414588763 5 44 11.747189928212968 5 45 11.011870366463896 5 46 12.75163657170096 5 47 13.367269979062772 5 48 13.098006804540663 5 49 13.226297279234853 5 50 12.14231914129235 5 51 11.696390499716557 5 52 11.818603355180837 5 53 11.91017023114178 5 54 11.401188861267745 5 55 12.06667744144017 5 56 11.89734059009232 6 1 3.6114864425492215 6 2 4.856415070835631 6 3 4.8693253511103975 6 4 4.917588417570151 6 5 4.783671688227024 6 6 5.431160082531065 6 7 5.0607653249038975 6 8 4.763334278341148 6 9 4.738435642058448 6 10 5.010915110985778 6 11 5.490788146181124 6 12 6.611969960024876 6 13 6.522469955987823 6 14 6.41515730323184 6 15 7.904079590657968 6 16 8.300213966671532 6 17 9.613924571875188 6 18 10.332132878287887 6 19 8.634090593244505 6 20 8.072175423773395 6 21 7.663650109308374 6 22 8.733846614345019 6 23 7.820386821687613 6 24 8.097425030308752 6 25 7.581899112685512 6 26 6.81650609330508 6 27 6.6284401508548285 6 28 6.805481613465997 6 29 6.736002645444849 6 30 6.942040789428489 6 31 6.087415502481303 6 32 6.544813232369364 6 33 6.490298639932455 6 34 7.043141573395086 6 35 6.1751121258372805 6 36 8.092547994794359 6 37 7.920852494667685 6 38 7.741949751951129 6 39 8.895246123990606 6 40 4.899010104197405 6 41 12.976017061197822 6 42 11.780546021488131 6 43 12.809247071057698 6 44 12.25259208907731 6 45 11.646524228008516 6 46 13.433909480864624 6 47 13.763228362416111 6 48 13.608811126269355 6 49 13.71469263737811 6 50 13.258565639891788 6 51 13.283425313642127 6 52 12.547494911803486 6 53 12.437581195527532 6 54 12.552449365469425 6 55 11.650911134917564 6 56 11.530393811380833 7 1 3.1815144537536995 7 2 4.581905266914063 7 3 4.676648280282066 7 4 4.383774892159984 7 5 4.5101622844655225 7 6 5.005990014503851 7 7 4.453322347713019 7 8 4.230521153091047 7 9 4.263645726092065 7 10 4.318037960125437 7 11 4.802712916089092 7 12 5.572354113686341 7 13 6.588696446584059 7 14 5.594829171979345 7 15 7.0009183261521635 7 16 7.457276791801579 7 17 8.502079833980034 7 18 9.345538240432745 7 19 7.940012962192089 7 20 6.931319081236381 7 21 7.550804971253154 7 22 8.158016282212166 7 23 6.86900626946673 7 24 8.217873209580457 7 25 6.510870701320968 7 26 6.656241987662824 7 27 5.32892034293166 7 28 5.327890577051431 7 29 5.570336378938251 7 30 6.142921141569346 7 31 4.90894411392588 7 32 5.293912872225306 7 33 4.695580752777595 7 34 4.491320845103775 7 35 5.04666074528506 7 36 6.593348314922409 7 37 6.608927724759717 7 38 7.646711656713034 7 39 7.873237320469197 7 40 7.638305822484718 7 41 12.2725356686408 7 42 11.096672472068366 7 43 10.588158635683548 7 44 10.72678176494766 7 45 10.134319346055733 7 46 11.547554939047902 7 47 12.163188346409708 7 48 11.802088437193728 7 49 12.216093197602202 7 50 11.581094651496432 7 51 10.675982336451248 7 52 10.175746212323695 7 53 10.24690492501933 7 54 10.513433759226924 7 55 10.209534584297316 7 56 11.264687528867828 8 1 3.532163804403048 8 2 4.900087085095883 8 3 4.794459040949967 8 4 4.92783797193735 8 5 5.075561171292982 8 6 5.347826749197729 8 7 4.806754629716735 8 8 4.624074028786779 8 9 4.598061310507646 8 10 4.42958712524603 8 11 5.269085828890574 8 12 6.105285468046265 8 13 6.229466390925431 8 14 5.990469246190845 8 15 7.213348752868303 8 16 7.918083841894347 8 17 9.308666104852016 8 18 9.196883323920687 8 19 8.582174372210641 8 20 7.523155815930259 8 21 7.962446900752223 8 22 8.978053387963556 8 23 7.436028532917561 8 24 8.453262819970067 8 25 6.731186100207793 8 26 6.345249408813103 8 27 6.534857263154294 8 28 5.972371096531955 8 29 6.601644356674797 8 30 6.713653980159329 8 31 5.334964522089148 8 32 5.794144783171504 8 33 5.9495232388629375 8 34 5.165022143805071 8 35 5.538080040275787 8 36 7.345667424384374 8 37 6.528918448321875 8 38 7.075283085284465 8 39 7.820825446628749 8 40 3.627174097067279 8 41 11.484038451572154 8 42 10.092266164090628 8 43 10.203186464997088 8 44 11.328126848435602 8 45 9.718271108578925 8 46 11.660959391738064 8 47 13.86193602730025 8 48 12.123517008622297 8 49 12.930378911887917 8 50 12.3679684919417 8 51 11.018050982091324 8 52 11.871247139967483 8 53 11.325058913887606 8 54 10.724240809319689 8 55 12.078272988750008 8 56 11.097015914767645 9 1 3.9407439882641313 9 2 5.115613132082601 9 3 4.97474504707867 9 4 5.333098441804566 9 5 5.341393653417597 9 6 5.597826749197729 9 7 5.122893546979242 9 8 5.022312026743883 9 9 4.794945886605703 9 10 5.275731149760837 9 11 5.840204317143893 9 12 6.6780638132965215 9 13 7.402729925144023 9 14 6.676117867232723 9 15 7.879845177791697 9 16 8.786444413906604 9 17 9.907491436823413 9 18 10.107634090008531 9 19 8.978880194478261 9 20 8.169223231660595 9 21 7.917120036605137 9 22 8.819217841487564 9 23 8.07022158705444 9 24 8.241950256129417 9 25 7.633892943925872 9 26 7.492976681540377 9 27 6.643641737107302 9 28 6.6609563876453315 9 29 7.704938534407177 9 30 7.4024946338876205 9 31 5.420638679392521 9 32 5.978388909831359 9 33 5.606954290956907 9 34 5.663873012037964 9 35 6.3614967920633205 9 36 7.724625544915526 9 37 7.91975093044649 9 38 8.064047130228285 9 39 8.619855068692079 9 40 5.87896163537167 9 41 11.862996572103306 9 42 11.81634685867694 9 43 11.341082277050205 9 44 11.078765254972886 9 45 11.105656195402211 9 46 13.003528339644095 9 47 13.820950327606685 9 48 12.75553948053241 9 49 13.643862057955335 9 50 13.182447552207282 9 51 11.665778254818598 9 52 11.969944790631427 9 53 11.57825094657402 9 54 12.303275538635315 9 55 11.801715276799037 9 56 12.500871890252835 10 1 3.749732752309079 10 2 4.789770435453391 10 3 4.828677631348334 10 4 4.849952374388835 10 5 5.296449833192874 10 6 5.103444726725819 10 7 4.7858148952938535 10 8 4.303210903148376 10 9 4.525282965257389 10 10 4.983596318300165 10 11 4.896384092424789 10 12 6.161209880712253 10 13 5.402729925144023 10 14 6.046904384086655 10 15 6.958496863184951 10 16 7.24711857120997 10 17 9.008615032329033 10 18 9.073926224839994 10 19 8.057531879871515 10 20 6.697313119301043 10 21 7.3890301489646895 10 22 7.909105481937004 10 23 7.182581137616232 10 24 7.275658121297955 10 25 6.656364854038237 10 26 5.751403647832511 10 27 5.935776568567981 10 28 5.672192342701511 10 29 6.7723542647442585 10 30 5.391258678831441 10 31 5.364458904111618 10 32 5.169400145786419 10 33 4.809201481968145 10 34 4.191962899678412 10 35 5.0468900504902905 10 36 7.117883971881817 10 37 7.313009357412781 10 38 8.142698815621547 10 39 7.990641585546012 10 40 11.204804332000883 10 41 11.548389830530276 10 42 10.861290678901664 10 43 11.172542951207511 10 44 11.415843906658274 10 45 10.184307880795473 10 46 10.834989013801394 10 47 11.259152574797692 10 48 10.710595660307693 10 49 11.565210372562074 10 50 10.182447552207279 10 51 10.070272636841068 10 52 10.11601220636176 10 53 9.229936339832445 10 54 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115 6 4.83592198729297 115 7 3.911372234334337 115 8 4.0672558469685995 115 9 4.0890425514888875 115 10 4.577675148334052 115 11 3.3843455691503124 115 12 0.03493914769994433 115 13 -0.9056346191755793 115 14 -3.1137876080660085 115 15 -3.3732313337117823 115 16 2.51963506844557 115 17 4.556501602687511 115 18 5.602907854945215 115 19 4.381056046092315 115 20 3.896171688946133 115 21 6.42041948372481 115 22 7.460737370647543 115 23 6.228416700305729 115 24 7.598825590532837 115 25 6.341936460958159 115 26 5.858056046619744 115 27 5.248421476718505 115 28 4.780271529432387 115 29 5.4773658573963 115 30 5.687138828644173 115 31 4.0710756332002624 115 32 4.366475230501955 115 33 4.397394811734507 115 34 4.546876400659325 115 35 5.663440790636535 115 36 7.1364322151491635 115 37 6.884437928841351 115 38 7.360997370998749 115 39 8.299541175344483 115 40 10.607693577586758 115 41 9.830358797892501 115 42 9.075130521954986 115 43 6.602897864708488 115 44 -0.6745787792700284 115 45 7.65586129616911 115 46 5.260706866485542 115 47 -6.198489658125437 115 48 3.579866214971503 115 49 -1.4426369611279561 115 50 4.666128665101876 115 51 8.157841746882088 115 52 7.95692535064569 115 53 7.09724506107375 115 54 7.623411083489962 115 55 7.6233667838437995 115 56 10.611626304378035 116 1 3.2876920599313095 116 2 4.041364726373523 116 3 4.191264937755868 116 4 4.158181952281332 116 5 4.977895323627131 116 6 4.17890783027881 116 7 3.659334482296586 116 8 4.2564450361577855 116 9 4.2829507453970805 116 10 4.155324225983129 116 11 4.220252905057649 116 12 4.501477114237911 116 13 5.312083098542139 116 14 4.688872194593795 116 15 -1.9684694289498772 116 16 4.93190448071498 116 17 6.455472001657917 116 18 6.1726204246577865 116 19 5.7403439053801755 116 20 5.590723383497824 116 21 6.441869505174832 116 22 7.233581643491817 116 23 6.379210351099381 116 24 6.822120313827565 116 25 6.355878974900669 116 26 6.182165870729563 116 27 5.730188958485989 116 28 5.580786329947184 116 29 6.754285634316076 116 30 7.169120810626154 116 31 5.459964522089148 116 32 5.7324125964393176 116 33 5.433859848199546 116 34 5.941127794910724 116 35 5.565414192609936 116 36 7.045913124630076 116 37 7.556252600656023 116 38 8.12933713933852 116 39 8.988515864319172 116 40 10.928156898050084 116 41 10.305905773439477 116 42 8.021934468758936 116 43 3.4624002242108496 116 44 2.540350435659189 116 45 8.53338167368949 116 46 2.1901362959149715 116 47 -5.907412867048645 116 48 1.724868359973648 116 49 -2.1236751421661353 116 50 1.9241724231456345 116 51 6.922535011575356 116 52 6.668851562571902 116 53 6.737528201356891 116 54 6.285358745437627 116 55 6.451123111600129 116 56 10.013170705922434 117 1 3.1458001680394148 117 2 4.115238600247395 117 3 4.106580253071179 117 4 4.329353123452506 117 5 4.9179854137172185 117 6 4.797826749197732 117 7 4.1264515994136985 117 8 3.983922513635264 117 9 4.337455249901588 117 10 4.23640530706421 117 11 2.2986312834360305 117 12 -4.200775138014343 117 13 -6.533412396953356 117 14 0.5631965189181152 117 15 -6.551802762283209 117 16 -7.014491915681411 117 17 2.0541206503065617 117 18 0.020368172405532903 117 19 1.3223258873621546 117 20 1.6064891492635915 117 21 5.878355991661319 117 22 6.95835641826659 117 23 6.245877017766048 117 24 7.113111304818556 117 25 6.138761857783557 117 26 5.659643348207041 117 27 5.733342111639139 117 28 5.6731286722895256 117 29 5.661492841523284 117 30 6.37047216197751 117 31 5.526631188755818 117 32 4.64187205589878 117 33 5.394220208559901 117 34 5.029416083199013 117 35 5.105504282700025 117 36 7.289606818323769 117 37 6.646342690746117 117 38 7.4586164186177974 117 39 8.501128476931786 117 40 10.286265006158189 117 41 7.861311178844879 117 42 5.752114648939113 117 43 -4.940752928942306 117 44 -0.8483883030795525 117 45 5.278877169184984 117 46 -1.433737577958901 117 47 -1.8691245787603563 117 48 -0.02648299137770138 117 49 -1.9696210881120813 117 50 -0.3267284777552675 117 51 5.4324449214852635 117 52 5.638671382391724 117 53 5.675816489645179 117 54 5.1083317184106 117 55 6.197969958446976 117 56 7.8901977329494635 118 1 2.695800168039412 118 2 3.590996176004971 118 3 3.5126408591317855 118 4 3.741474335573713 118 5 4.261924807656612 118 6 4.270554021925001 118 7 3.6522091751712793 118 8 3.0490740287867837 118 9 3.466243128689463 118 10 3.8454962161551194 118 11 0.7895403743451155 118 12 -7.2810781683173715 118 13 -6.2046245181654776 118 14 -8.241348935627336 118 15 -8.286651247131694 118 16 -7.561461612651108 118 17 0.45866610485201775 118 18 -6.237207585170225 118 19 -0.7291892641529962 118 20 -1.2132078204333787 118 21 3.473810537115863 118 22 4.605326115236288 118 23 3.91557398746302 118 24 4.555535547242794 118 25 3.6539133729350652 118 26 5.056613045176739 118 27 4.12576635406338 118 28 3.8928256419864944 118 29 4.667553447583892 118 30 4.820472161977506 118 31 2.7781463402709683 118 32 3.1736902377169613 118 33 2.9517959661356628 118 34 3.562749416532345 118 35 3.4948982220939655 118 36 3.8365765152934657 118 37 4.385736630140052 118 38 4.0025558125571905 118 39 5.770825446628752 118 40 7.072628642521828 118 41 5.356765724299425 118 42 5.226357073181539 118 43 0.039550101360724454 118 44 0.18949048479923647 118 45 4.886452926760743 118 46 -2.141313335534658 118 47 -2.3130639726997515 118 48 -0.9537557186504309 118 49 -2.7878029062939014 118 50 -0.45248605351284255 118 51 2.2112328002731445 118 52 3.1280653217856624 118 53 2.077331641160331 118 54 3.3128771729560533 118 55 2.9737275342045493 118 56 6.040197732949462 119 1 2.816435088674332 119 2 3.9073020923108857 119 3 3.760548507039431 119 4 3.8849086790080563 119 5 4.290207635939446 119 6 4.502588653959634 119 7 3.514546837508938 119 8 3.68630346601622 119 9 3.755709218155552 119 10 3.5618021324610325 119 11 0.9399011247058695 119 12 -3.0444259316651348 119 13 -1.5881743017152594 119 14 -5.33600983028823 119 15 -9.579580540060988 119 16 -6.654968106157604 119 17 -0.014926968741058033 119 18 -4.730425478388117 119 19 -0.761801096764831 119 20 -0.4689076761332309 119 21 3.8807369440422654 119 22 4.873435783345954 119 23 3.974448446337476 119 24 3.9956509873582355 119 25 4.722888841910539 119 26 5.127897316461009 119 27 4.53413576243279 119 28 4.954874704035557 119 29 4.85831823834868 119 30 4.845868987374331 119 31 3.896472458597085 119 32 3.5093323733590935 119 33 3.5402519545916533 119 34 4.4516383054212305 119 35 4.9174090446047884 119 36 4.993575072292018 119 37 5.201898246301667 119 38 5.932425942427319 119 39 6.6170014928048 119 40 8.845788815681999 119 41 6.179565147098849 119 42 5.67830512512959 119 43 -1.8732926114819861 119 44 -3.325372430063677 119 45 5.7828454231532405 119 46 -1.1202455144668377 119 47 -2.547696007331787 119 48 -4.229657594552307 119 49 -4.029938548429541 119 50 0.5391445381177498 119 51 4.99911158815193 119 52 5.290258683979026 119 53 4.732165695994386 119 54 4.29007775015663 119 55 5.829715990193005 119 56 6.167181859933589 120 1 2.816435088674332 120 2 4.066032251041044 120 3 3.951024697515628 120 4 4.18649598059536 120 5 4.671160016891825 120 6 4.550207701578678 120 7 4.038356361318463 120 8 3.781541561254315 120 9 4.1049155673619 120 10 4.339579910238811 120 11 0.812916997721743 120 12 -5.822203709442913 120 13 -4.270713984254943 120 14 -5.097914592192993 120 15 -0.34148530196575066 120 16 -6.5121109633004615 120 17 0.9374539836398945 120 18 -5.651060399023038 120 19 0.5397862048224731 120 20 -0.00859021581577224 120 21 3.563276626581949 120 22 5.413118323028492 120 23 4.46651193840097 120 24 4.709936701643951 120 25 5.056222175243867 120 26 6.74694493550863 120 27 5.391278619575651 120 28 5.129477878638735 120 29 6.366254746285186 120 30 6.464916606421951 120 31 4.832980395105022 120 32 5.541078405105125 120 33 6.064061478401172 120 34 4.911955765738693 120 35 5.695186822382567 120 36 6.866590945307891 120 37 6.043168087571509 120 38 6.868933878935255 120 39 6.9027157785190845 120 40 9.06801103790422 120 41 -4.852180884647183 120 42 -3.829631382806916 120 43 -5.333610071799448 120 44 -14.43648354117479 120 45 -3.248900608592791 120 46 -7.977388371609695 120 47 -4.357219816855597 120 48 -3.689975054869766 120 49 -13.363271881762877 120 50 -2.5084745095012977 120 51 2.0308576198979615 120 52 1.7505761442964847 120 53 2.2718482356769236 120 54 2.1630936231725038 120 55 1.7186048790818944 120 56 6.70686439961613 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 2126.0 5 296.0 6 123.0 7 268.0 8 404.0 9 340.0 10 286.0 11 223.0 12 185.0 13 151.0 14 176.0 15 242.0 16 301.0 17 373.0 18 492.0 19 574.0 20 757.0 21 949.0 22 1177.0 23 1332.0 24 1659.0 25 1881.0 26 1914.0 27 2105.0 28 2148.0 29 2220.0 30 2422.0 31 2784.0 32 3204.0 33 3686.0 34 4087.0 35 4309.0 36 4726.0 37 5269.0 38 5576.0 39 6053.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 0.0 100.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.6667695155816 0.23912372724467756 0.05862388151804998 0.03548287565566183 8 99.11447084233261 0.2730638691761802 0.1388460351743289 0.4736192533168775 9 97.08577599506324 0.8762727553224313 1.0413452638074667 0.9966059858068498 10 43.73033014501697 30.672631903733418 17.96359148410984 7.633446467139772 11 27.679728478864547 29.335081764887384 20.887071891391546 22.098117864856526 12 26.322122801604444 25.29003394014193 19.75007713668621 28.637766121567417 13 20.502931194075902 19.136069114470843 23.395556926874423 36.96544276457883 14 21.788028386300525 29.90126504165381 26.752545510644865 21.558161061400803 15 32.267572640821584 21.219031662555654 22.145173050371543 24.368222646251223 16 27.476107397057238 30.22433571616051 22.515400886226438 19.78415600055582 17 24.87848534880491 22.554662304997915 33.376024194917214 19.190828151279955 18 22.084233261339094 21.925331687750692 26.070657204566494 29.91977784634372 19 25.9950306341343 23.349846443508188 22.55968640523481 28.095436517122707 20 21.41401037805782 29.33191252779837 29.40295280454658 19.851124289597234 21 21.26812712125887 22.48225856217217 28.933970996605986 27.315643319962973 22 21.22801604443073 22.81548904659056 30.518358531317496 25.438136377661213 23 20.24992286331379 24.989200863930886 29.734649799444618 25.026226473310707 24 19.4300877856118 25.281947637193948 27.59306971936375 27.69489485783051 25 25.30899439875322 25.10068356813307 28.88731155585045 20.70301047726326 26 30.404519006682822 25.834580896084454 19.960489559057308 23.80041053817542 27 21.58945304660605 31.492659431588372 21.032156475292158 25.88573104651342 28 25.901356083864297 33.400700411916254 20.555701260432897 20.142242243786544 29 31.03155227293798 28.567013813776693 20.457986958805897 19.943446954479434 30 25.683017935973822 25.43450745531442 29.586639088692003 19.295835520019757 31 27.05012883217873 33.263388517735635 20.77052443182695 18.915958218258684 32 31.670164824989193 28.193098339403665 21.323847151058708 18.812889684548427 33 27.09115343621113 28.269768447719233 21.701654386208812 22.93742372986082 34 22.711743882267466 29.154879500378733 30.21224628607646 17.921130331277343 35 21.65858030223431 26.677276800890017 33.851478723075495 17.81266417380018 36 25.371245098947238 29.965113766169615 25.246210367077275 19.41743076780587 37 25.369260243495457 25.92701316358692 30.20363389160126 18.50009270131636 38 29.966293524645927 25.927701156534113 25.575174717051148 18.530830601768816 39 24.78518884836496 25.642888050936513 23.19342276070965 26.378500339988875 40 21.55116025541193 20.789596636038 27.746456938171626 29.912786170378446 41 18.172874209416726 18.126024830171 25.2471304755212 38.45397048489107 42 24.226437279534625 28.088062380097778 24.122779874992265 23.562720465375335 43 18.631002136156773 25.372279495990835 30.184823999256988 25.8118943685954 44 19.489541176288164 26.561845838954273 27.208447690375404 26.740165294382162 45 23.007700157714073 25.44762965024585 31.20419333889971 20.340476853140366 46 19.25209043047383 29.224218024156084 24.66862805822236 26.855063487147724 47 17.972350230414747 27.98957315086347 23.15163928067154 30.886437338050243 48 23.399623259117437 29.17734613840595 24.46036500633048 22.962665596146124 49 18.631214015972265 30.457190614746487 23.54670958954993 27.364885779731317 50 23.21450655453871 29.390304229532525 24.54705664110809 22.848132574820678 51 19.67253706397598 32.65808288712124 24.773282986164848 22.896097062737937 52 19.961677535000156 32.25731680934574 28.500788082949597 19.280217572704515 53 19.625922430730675 30.362474666996704 29.2826312288247 20.72897167344792 54 19.93317451969186 29.885839804473598 27.625839185719148 22.555146490115398 55 19.643520237653174 28.70250030944424 28.654536452531254 22.999443000371333 56 22.712997199746276 25.947986447390814 29.377910484706902 21.961105868156007 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 1.0 2 2.0 3 4.5 4 10.0 5 13.0 6 151.5 7 290.0 8 372.0 9 454.0 10 452.5 11 451.0 12 405.5 13 332.0 14 304.0 15 311.0 16 318.0 17 324.0 18 330.0 19 326.5 20 323.0 21 351.5 22 401.5 23 423.0 24 502.0 25 581.0 26 639.5 27 698.0 28 730.5 29 838.5 30 914.0 31 1099.5 32 1285.0 33 1288.0 34 1291.0 35 1367.0 36 1443.0 37 1588.0 38 1926.5 39 2120.0 40 2630.5 41 3141.0 42 4360.0 43 5579.0 44 5547.0 45 5515.0 46 4719.5 47 3804.0 48 3684.0 49 3688.5 50 3693.0 51 3695.5 52 3698.0 53 3551.5 54 3441.5 55 3478.0 56 3370.0 57 3262.0 58 3054.5 59 2847.0 60 2574.5 61 2302.0 62 2067.0 63 1596.5 64 1361.0 65 1132.5 66 904.0 67 768.5 68 633.0 69 537.0 70 441.0 71 357.0 72 214.5 73 156.0 74 127.0 75 98.0 76 85.5 77 73.0 78 51.5 79 16.0 80 2.0 81 2.5 82 3.0 83 2.0 84 1.0 85 1.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5538414069731564 16 0.07867941993211972 17 0.02005553841406973 18 0.0 19 0.03548287565566183 20 0.10490589324282629 21 0.0 22 0.0 23 0.0 24 0.0046282011724776305 25 0.02005553841406973 26 0.04165381055229867 27 0.06633755013884604 28 0.0015427337241592102 29 0.15735883986423943 30 0.05245294662141314 31 0.01079913606911447 32 0.037025609379821044 33 0.12804689910521444 34 0.20209811786485654 35 0.15735883986423943 36 0.05862388151804998 37 0.14810243751928417 38 0.22215365627892628 39 0.17278617710583152 40 0.9410675717371182 41 1.2110459734649799 42 0.2838630052452946 43 0.3363159518667078 44 0.5075593952483801 45 0.22523912372724467 46 0.37025609379821045 47 0.572354211663067 48 0.08330762110459734 49 0.3208886146251157 50 0.20364085158901574 51 0.3116322122801604 52 0.16352977476087627 53 0.27923480407281703 54 0.26843566800370255 55 0.2900339401419315 56 0.2823202715211354 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 64820.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.27306386917618 #Duplication Level Percentage of deduplicated Percentage of total 1 89.70937858665356 67.52699784017278 2 6.32275782915232 9.518667078062325 3 1.7502869322839811 3.952483801295896 4 0.7337268404656501 2.2091946929959887 5 0.3853090670601738 1.4501697007096577 6 0.2582390555828824 1.1663066954643628 7 0.161911788817839 0.8531317494600432 8 0.12502049516314148 0.7528540573896945 9 0.0983767830791933 0.6664609688367787 >10 0.4078537465158223 5.044739278000617 >50 0.02664371208394819 1.4578833693304536 >100 0.014346614199049024 1.9268744214748537 >500 0.004099032628299721 1.681579759333539 >1k 0.0020495163141498606 1.7926565874730023 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 1162 1.7926565874730023 No Hit ACTTATGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT 567 0.8747300215982721 No Hit ACTTATGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC 523 0.8068497377352669 No Hit ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 325 0.5013884603517432 No Hit ACTTATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 186 0.28694847269361307 No Hit ACTTATGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA 184 0.2838630052452946 No Hit ACTTATGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC 163 0.25146559703795124 No Hit ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 143 0.22061092255476702 No Hit ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG 130 0.20055538414069735 No Hit ACTTATGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT 118 0.1820425794507868 No Hit ACTTATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 97 0.1496451712434434 No Hit ACTTATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA 96 0.14810243751928417 No Hit ACTTATGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC 87 0.13421783400185128 No Hit ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG 83 0.12804689910521444 No Hit ACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 80 0.12341869793273681 No Hit ACTTATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 78 0.12033323048441838 No Hit ACTTATGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC 66 0.10182042579450787 No Hit ACTTATGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT 66 0.10182042579450787 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.01079913606911447 0.0 0.0 0.0 0.0 11 0.015427337241592102 0.0 0.0 0.0 0.0 12 0.016970070965751313 0.0 0.0 0.0 0.0 13 0.018512804689910522 0.0 0.0 0.0 0.0 14 0.04165381055229867 0.0 0.0 0.0 0.0 15 0.04165381055229867 0.0 0.0 0.0 0.0 16 0.04319654427645788 0.0 0.0 0.0 0.0 17 0.046282011724776306 0.0 0.0 0.0 0.0 18 0.05245294662141314 0.0 0.0 0.0 0.0 19 0.05553841406973156 0.0 0.0 0.0 0.0 20 0.06633755013884604 0.0 0.0 0.0 0.0 21 0.07096575131132367 0.0 0.0 0.0 0.0 22 0.07405121875964209 0.0 0.0 0.0 0.0 23 0.0771366862079605 0.0 0.0 0.0 0.0 24 0.08485035482875655 0.0 0.0 0.0 0.0 25 0.09564949089787103 0.0 0.0 0.0 0.0 26 0.10027769207034866 0.0 0.0 0.0 0.0 27 0.10953409441530391 0.0 0.0 0.0 0.0 28 0.12341869793273681 0.0 0.0 0.0 0.0 29 0.12650416538105522 0.0 0.0 0.0 0.0 30 0.12958963282937366 0.0 0.0 0.0 0.0 31 0.13267510027769208 0.0 0.0 0.0 0.0 32 0.1373033014501697 0.0 0.0 0.0 0.0 33 0.14038876889848811 0.0 0.0 0.0 0.0 34 0.14655970379512495 0.0 0.0 0.0 0.0 35 0.1527306386917618 0.0 0.0 0.0 0.0 36 0.154273372415921 0.0 0.0 0.0 0.0 37 0.15735883986423943 0.0 0.0 0.0 0.0 38 0.15735883986423943 0.0 0.0 0.0 0.0 39 0.16198704103671707 0.0 0.0 0.0 0.0 40 0.1681579759333539 0.0 0.0 0.0 0.0 41 0.17587164455414994 0.0 0.0 0.0 0.0 42 0.1820425794507868 0.0 0.0 0.0015427337241592102 0.0 43 0.18821351434742364 0.0 0.0 0.0015427337241592102 0.0 44 0.2020981178648565 0.0 0.0 0.0015427337241592102 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCTCA 30 9.3559356E-7 50.717323 41 CCCCGAC 15 0.00793502 50.717323 41 GCATTGA 20 3.91735E-4 50.5978 40 TCGTCTC 25 1.9249166E-5 50.5978 40 TGCTCGT 25 1.9249166E-5 50.5978 37 TGCCCCA 15 0.008009459 50.597797 38 TTGTCCA 15 0.008009459 50.597797 37 AGACCGC 15 0.008009459 50.597797 37 CTACCAC 15 0.008009459 50.597797 38 CGTCGAG 15 0.008009459 50.597797 38 TCGAGCA 15 0.008009459 50.597797 40 GCTACCA 15 0.008009459 50.597797 37 GCGTCGA 15 0.008009459 50.597797 37 CGCATTG 20 3.9325817E-4 50.558086 39 GTCGAGC 15 0.008034387 50.558083 39 ATGCTCG 25 1.9519608E-5 50.47884 36 ACGGCTA 15 0.008159893 50.360435 34 GTCATCA 15 0.008210502 50.28181 46 TTGGATT 15 0.008210502 50.28181 44 TCCACGA 25 1.9977337E-5 50.28181 45 >>END_MODULE