FastQCFastQC Report
Fri 17 Jun 2016
SRR1526021_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526021_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19402
Sequences flagged as poor quality0
Sequence length40
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTATGGGAATTGACGGGTACGCACAGTTCTGAGTCGGA1370.7061127718791878No Hit
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT1320.6803422327595093No Hit
ACTTATGGGTACCTGGTTGATCCTGCCCGTAGCATATGCT1280.6597258014637666No Hit
ACTTATGGGTACCTGGTTGATCCTGCCCGTAGCCTATGCT1100.5669518606329245No Hit
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCCTATGCT860.44325327285846816No Hit
ACTTATGGGAATTGACGGGTACGCACCGTTCTGAGTCGGA630.32470879290794763No Hit
ACTTATGGGTACCTGGTTGATCCTGCCCGTAGCCTCTGCT490.25255128337284816No Hit
ACTTATGGGAATTGACGGGTACGCACAGTTCTGAGTCCGA310.15977734254200598No Hit
ACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC310.15977734254200598No Hit
ACTTATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG310.15977734254200598No Hit
ACTTATGGGTACCTGGTTGATCCTGCCCGTCGCCTATGCT280.14431501907019895No Hit
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATTCT240.12369858777445625No Hit
ACTTATGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCG230.11854447995052056No Hit
ACTTATGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC220.11339037212658488No Hit
ACTTATGGGAATTGACGGGTACGCACCGTTCTGAGTCCGA220.11339037212658488No Hit
ACTTATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC220.11339037212658488No Hit
ACTTATGGGAATTGACGGGTACGCACAGTTCTGCGTCGGA200.10308215647871354No Hit
ACTTATGGGTACCTGGTTGATCCTGCCCGTAGCATCTGCT200.10308215647871354No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGGT200.002673330734.1246763
ATGGGTG251.9136493E-434.1246765
ATGGGTA2200.034.1246765
TTATGCG200.002673330734.1246763
ATGGGCC200.002673330734.1246765
CCCAGTT200.002673330734.12467624
CAGTTCT407.1535396E-834.12467626
CCAGTAG601.8189894E-1234.12467626
CCTGCCA800.034.12467622
TAGCTCC200.002673330734.12467623
CCGTTCT359.905343E-734.12467626
GCCAGTC200.002673330734.12467625
TGGGGTT301.3747098E-534.1246766
TGGGGTG750.034.1246766
TGGGGTA359.905343E-734.1246766
TGGGGGC601.8189894E-1234.1246766
TGGGGCT359.905343E-734.1246766
TGGGGAC301.3747098E-534.1246766
TGGGGAA503.7471182E-1034.1246766
TGCCAGT800.034.12467624
Produced by FastQC (version 0.11.5)