##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526021_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 19402 Sequences flagged as poor quality 0 Sequence length 40 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 23.164003710957633 26.0 19.0 30.0 4.0 33.0 2 12.471652406968353 11.0 10.0 13.0 4.0 21.0 3 15.375476754973715 15.0 11.0 17.0 4.0 30.0 4 19.20085558189877 20.0 11.0 27.0 4.0 32.0 5 19.26059169157819 22.0 10.0 28.0 4.0 31.0 6 20.704978868157923 24.0 11.0 29.0 4.0 32.0 7 17.6987939387692 18.0 9.0 27.0 4.0 31.0 8 20.140604061436964 23.0 10.0 29.0 4.0 32.0 9 17.644675806617876 18.0 9.0 27.0 4.0 31.0 10 17.47206473559427 19.0 7.0 26.0 4.0 31.0 11 22.761107102360583 27.0 9.0 35.0 4.0 38.0 12 21.618286774559323 24.0 4.0 33.0 4.0 37.0 13 21.94315019070199 25.0 4.0 33.0 4.0 37.0 14 22.37815689104216 27.0 4.0 34.0 4.0 37.0 15 21.424543861457582 25.0 4.0 33.0 4.0 37.0 16 19.439645397381714 20.0 4.0 32.0 4.0 36.0 17 20.04149056798268 23.0 4.0 33.0 4.0 36.0 18 19.802958457890938 23.0 4.0 33.0 4.0 36.0 19 17.69956705494279 17.0 4.0 30.0 4.0 35.0 20 18.468508401195752 19.0 4.0 32.0 4.0 36.0 21 16.545304607772394 13.0 4.0 30.0 4.0 35.0 22 15.421554478919699 10.0 4.0 28.0 4.0 33.0 23 15.507885784970622 8.0 4.0 29.0 4.0 34.0 24 15.280538088856819 4.0 4.0 30.0 4.0 34.0 25 14.377280692712091 4.0 4.0 28.0 4.0 33.0 26 11.789763941861663 4.0 4.0 21.0 4.0 32.0 27 11.651015359241315 4.0 4.0 20.0 4.0 32.0 28 11.289815482939902 4.0 4.0 19.0 4.0 31.0 29 10.917585815895269 4.0 4.0 18.0 4.0 31.0 30 10.246160189671167 4.0 4.0 14.0 4.0 31.0 31 9.321616328213587 4.0 4.0 4.0 4.0 30.0 32 8.878517678589835 4.0 4.0 4.0 4.0 30.0 33 8.397948665086073 4.0 4.0 4.0 4.0 28.0 34 7.994794351097825 4.0 4.0 4.0 4.0 26.0 35 7.569065044840738 4.0 4.0 4.0 4.0 24.0 36 7.228739305226266 4.0 4.0 4.0 4.0 21.0 37 7.106277703329553 4.0 4.0 4.0 4.0 20.0 38 6.803937738377487 4.0 4.0 4.0 4.0 17.0 39 6.766312751262756 4.0 4.0 4.0 4.0 17.0 40 6.683950108236265 4.0 4.0 4.0 4.0 17.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 10.366327117429929 1 2 22.999461695218162 1 3 20.13152643395344 1 4 16.579145525927952 1 5 13.515149505301633 1 6 13.16124265978615 1 7 15.881237328078615 1 8 7.769977679490314 1 9 8.701018891339931 1 10 10.799049122159385 1 11 2.3215601205882663 1 12 3.6048840250512235 1 13 -3.000269685004973 1 14 1.6574449409976708 1 15 -3.238437777619726 1 16 -1.0970058988599405 1 17 0.5370281489883766 1 18 -0.5597407361024089 1 19 -1.8593451287768072 1 20 1.1572075533130892 1 21 2.794399032436516 1 22 0.6560403770390177 1 23 2.489018008463429 1 24 0.614677827548217 1 25 3.3734541996364236 1 26 -0.0480593797433535 1 27 1.4872663414177882 1 28 1.8077951110918473 1 29 0.9798302655354227 1 30 0.4856518363002511 1 31 1.9803338785090006 1 32 1.9378863909552848 1 33 1.2484209345671688 1 34 2.094815221029844 1 35 1.462008806279866 1 36 0.919505811276057 1 37 1.2155164973790278 1 38 1.2027675978892098 1 39 2.1686667438888367 1 40 -0.6165364435861287 2 1 7.65521600631882 2 2 25.266128361884828 2 3 22.909304211731218 2 4 19.423589970372394 2 5 14.3818161719683 2 6 16.22790932645282 2 7 18.45901510585639 2 8 7.52553323504587 2 9 12.323241113562155 2 10 -12.289839766729504 2 11 -16.611773212745067 2 12 -15.52844930828211 2 13 -16.222491907227194 2 14 -16.786999503446772 2 15 -16.28288222206417 2 16 -13.874783676637719 2 17 -14.32963851767829 2 18 -14.15974073610241 2 19 -12.148234017665697 2 20 -12.509459113353579 2 21 -10.827823189785708 2 22 -9.899515178516538 2 23 -9.666537547092126 2 24 -9.274211061340672 2 25 -8.182101355919132 2 26 -6.51472604641002 2 27 -6.6016225474711 2 28 -6.081093777797042 2 29 -5.664614178909021 2 30 -5.181014830366415 2 31 -4.064110565935444 2 32 -3.7732247201558256 2 33 -3.4626901765439415 2 34 -3.216295890081268 2 35 -2.8046578603868015 2 36 -2.613827522057276 2 37 -2.6733723915098615 2 38 -2.286121290999679 2 39 -2.29799992277783 2 40 -2.172091999141684 3 1 -18.34478399368118 3 2 -8.733871638115172 3 3 -11.090695788268784 3 4 -14.576410029627606 3 5 -14.6181838280317 3 6 -15.77209067354718 3 7 -13.540984894143609 3 8 -15.47446676495413 3 9 -13.676758886437845 3 10 -12.289839766729504 3 11 -16.611773212745067 3 12 -15.52844930828211 3 13 -16.222491907227194 3 14 -16.786999503446772 3 15 -16.28288222206417 3 16 -13.874783676637719 3 17 -14.32963851767829 3 18 -14.15974073610241 3 19 -12.148234017665697 3 20 -12.509459113353579 3 21 -10.827823189785708 3 22 -9.899515178516538 3 23 -9.666537547092126 3 24 -9.274211061340672 3 25 -8.182101355919132 3 26 -6.51472604641002 3 27 -6.6016225474711 3 28 -6.081093777797042 3 29 -5.664614178909021 3 30 -5.181014830366415 3 31 -4.064110565935444 3 32 -3.7732247201558256 3 33 -3.4626901765439415 3 34 -3.216295890081268 3 35 -2.8046578603868015 3 36 -2.613827522057276 3 37 -2.6733723915098615 3 38 -2.286121290999679 3 39 -2.29799992277783 3 40 -2.172091999141684 5 1 -5.14478399368118 5 2 -5.308871638115172 5 3 -3.3906957882687845 5 4 -6.826410029627606 5 5 -6.0931838280317 5 6 -10.77209067354718 5 7 -11.865984894143608 5 8 -12.89946676495413 5 9 -11.551758886437845 5 10 -8.614839766729503 5 11 -9.411773212745068 5 12 -10.77844930828211 5 13 -11.172491907227194 5 14 -11.886999503446772 5 15 -8.88288222206417 5 16 -7.274783676637719 5 17 -8.52963851767829 5 18 -8.85974073610241 5 19 -7.848234017665696 5 20 -6.259459113353579 5 21 -7.027823189785708 5 22 -6.324515178516537 5 23 -7.366537547092126 5 24 -6.024211061340672 5 25 -6.007101355919132 5 26 -4.3397260464100205 5 27 -4.3266225474711 5 28 -3.6060937777970423 5 29 -3.289614178909021 5 30 -2.681014830366415 5 31 -2.5641105659354437 5 32 -2.4482247201558254 5 33 -1.9876901765439419 5 34 -1.5162958900812677 5 35 -1.2546578603868017 5 36 -2.213827522057276 5 37 -1.7483723915098617 5 38 -1.661121290999679 5 39 -2.0229999227778297 5 40 -1.8970919991416837 6 1 -0.5447839936811789 6 2 -0.43387163811517127 6 3 -3.0906957882687838 6 4 1.3235899703723923 6 5 -3.1181838280317002 6 6 0.92790932645282 6 7 2.8590151058563897 6 8 1.5255332350458701 6 9 2.023241113562154 6 10 3.610160233270495 6 11 4.588226787254932 6 12 9.771550691717891 6 13 5.477508092772805 6 14 3.5130004965532287 6 15 9.01711777793583 6 16 3.9252163233622817 6 17 8.870361482321709 6 18 10.440259263897591 6 19 9.951765982334305 6 20 7.090540886646423 6 21 9.572176810214291 6 22 9.000484821483461 6 23 8.633462452907875 6 24 8.32578893865933 6 25 7.91789864408087 6 26 10.28527395358998 6 27 6.598377452528899 6 28 8.618906222202957 6 29 8.335385821090979 6 30 6.018985169633584 6 31 2.1358894340645556 6 32 1.4267752798441737 6 33 1.5373098234560585 6 34 2.6837041099187324 6 35 0.6953421396131985 6 36 1.2861724779427242 6 37 0.8266276084901385 6 38 3.213878709000321 6 39 3.4020000772221692 6 40 2.0279080008583152 7 1 3.6097614608642736 7 2 2.0388556346121014 7 3 -1.0906957882687838 7 4 2.9235899703723938 7 5 0.5181798083319364 7 6 1.591545690089184 7 7 4.368106014947301 7 8 2.02553323504587 7 9 5.550513840834881 7 10 7.57379659690686 7 11 7.9336813327094795 7 12 8.698823418990617 7 13 11.868417183681899 7 14 9.803909587462318 7 15 9.171663232481283 7 16 9.534307232453191 7 17 11.988543300503526 7 18 10.7493501729885 7 19 9.98812961869794 7 20 10.808722704828238 7 21 12.535813173850656 7 22 12.009575730574372 7 23 11.37891699836242 7 24 11.862152575022964 7 25 9.999716825899052 7 26 6.439819408135433 7 27 4.3983774525289 7 28 6.282542585839321 7 29 5.108113093818252 7 30 2.0008033514517667 7 31 2.2086167067918296 7 32 1.3631389162078111 7 33 3.12821891436515 7 34 1.9200677462823688 7 35 4.468069412340472 7 36 2.158899750669997 7 37 2.7811730630355935 7 38 2.5320605271821393 7 39 2.3838182590403516 7 40 2.2369989099492242 9 1 1.5870341881370038 9 2 1.3797647255211913 9 3 -0.3179685155415104 9 4 0.2190445158269405 9 5 -0.4590929189407902 9 6 4.841545690089184 9 7 4.459015105856391 9 8 2.4573514168640536 9 9 2.6414229317439712 9 10 5.437432960543223 9 11 7.615499514527659 9 12 8.676096146263344 9 13 7.0275080927728055 9 14 7.576636860189591 9 15 5.262572323390376 9 16 8.71612541427137 9 17 10.238543300503526 9 18 10.59025926389759 9 19 9.715402345970666 9 20 10.104177250282785 9 21 11.581267719305202 9 22 7.623212094210736 9 23 14.015280634726057 9 24 12.975788938659328 9 25 13.204262280444505 9 26 4.121637589953616 9 27 4.352922907074355 9 28 4.25981531311205 9 29 2.949022184727342 9 30 2.318985169633585 9 31 1.2995257977009196 9 32 2.2722298252987194 9 33 2.764582550728785 9 34 2.3064313826460054 9 35 1.6953421396131985 9 36 2.704354296124542 9 37 2.940263972126502 9 38 2.259333254454866 9 39 1.5656364408585333 9 40 1.9642716372219526 10 1 2.3475236986265138 10 2 -0.34925625349978695 10 3 11.5439195963466 10 4 1.2312822780647004 10 5 0.18950847966060635 10 6 5.381755480298974 10 7 4.3628612597025445 10 8 6.79476400427664 10 9 3.611702652023695 10 10 4.88323715634742 10 11 8.157457556485703 10 12 12.64462761479481 10 13 9.604431169695882 10 14 10.443769727322458 10 15 8.2363485471666 10 16 9.471370169516128 10 17 12.43959225155248 10 18 12.705643879282206 10 19 13.640227520795843 10 20 13.125156271261805 10 21 13.114484502521986 10 22 17.773561744560386 10 23 16.39115476060018 10 24 15.552712015582404 10 25 16.587129413311636 10 26 7.619889338205363 10 27 6.475300529451976 10 28 9.111213914510651 10 29 9.662308898014055 10 30 7.472831323479738 10 31 7.435889434064556 10 32 4.7267752798441744 10 33 3.691155977302212 10 34 4.360627186841809 10 35 3.483803678074737 10 36 4.309249401019647 10 37 5.0766276084901385 10 38 3.079263324384936 10 39 4.086615461837555 10 40 2.8663695393198543 11 1 -1.026602175499363 11 2 1.266128361884828 11 3 -0.7725139700869654 11 4 6.15086269764512 11 5 0.8818161719682998 11 6 4.909727508271004 11 7 1.7771969240382077 11 8 4.7982605077731435 11 9 2.4596047499257914 11 10 4.619251142361406 11 11 9.979135878164023 11 12 9.426096146263344 11 13 10.595689910954622 11 14 8.440273223825955 11 15 8.762572323390376 11 16 10.534307232453191 11 17 11.67036148232171 11 18 12.294804718443043 11 19 11.715402345970666 11 20 11.899631795737331 11 21 13.71763135566884 11 22 14.736848457847099 11 23 12.46982608927151 11 24 12.043970756841144 11 25 11.499716825899052 11 26 4.258001226317253 11 27 2.307468361619808 11 28 3.964360767657503 11 29 -0.5282505425453845 11 30 1.27353062417904 11 31 0.34498034315546455 11 32 0.4540480071169011 11 33 0.12821891436514932 11 34 0.42006774628236876 11 35 2.87716032143138 11 36 0.6588997506699963 11 37 1.3720821539446835 11 38 0.12296961809123008 11 39 0.6110909863130791 11 40 0.9642716372219526 12 1 3.4766445777473933 12 2 1.6232712190276857 12 3 1.8378756403026433 12 4 4.923589970372394 12 5 3.6675304576825845 12 6 3.4064807550242477 12 7 -2.4695563227150377 12 8 2.954104663617297 12 9 -2.033901743580703 12 10 5.210160233270496 12 11 11.138226787254933 12 12 11.614407834575033 12 13 8.170365235629948 12 14 8.570143353696086 12 15 9.288546349364402 12 16 8.303787751933708 12 17 9.277504339464567 12 18 10.483116406754732 12 19 11.637480268048588 12 20 10.311969458074994 12 21 9.636462524500008 12 22 6.529056250054889 12 23 11.2620338814793 12 24 9.86864608151647 12 25 7.960755786938011 12 26 5.270988239304266 12 27 2.719806023957471 12 28 2.5260490793458157 12 29 3.8711001068052653 12 30 4.211842312490727 12 31 1.9358894340645563 12 32 1.1553467084156033 12 33 2.001595537741772 12 34 2.1765612527758744 12 35 -0.5903721461010871 12 36 -0.5066846649144194 12 37 -1.7805152486527183 12 38 -1.286121290999679 12 39 -0.33371420849211564 12 40 -1.172091999141684 13 1 1.6552160063188204 13 2 -0.40053830478183805 13 3 -0.3906957882687845 13 4 6.856923303705727 13 5 0.5484828386349676 13 6 0.1945759931194857 13 7 -2.8743182274769428 13 8 -0.9411334316207949 13 9 -0.4100922197711796 13 10 5.0434935666038285 13 11 9.788226787254931 13 12 5.771550691717891 13 13 9.044174759439471 13 14 8.446333829886562 13 15 8.050451111269162 13 16 9.891882990028947 13 17 9.637028148988374 13 18 9.073592597230924 13 19 10.985099315667636 13 20 14.323874219979754 13 21 12.405510143547627 13 22 14.033818154816796 13 23 15.166795786241206 13 24 14.492455605325993 13 25 14.551231977414202 13 26 12.551940620256646 13 27 12.8983774525289 13 28 7.0189062222029595 13 29 13.068719154424313 13 30 12.518985169633584 13 31 6.26922276739789 13 32 4.793441946510841 13 33 6.970643156789392 13 34 3.117037443252066 13 35 4.6953421396131985 13 36 2.3195058112760574 13 37 2.2932942751568053 13 38 2.1472120423336545 13 39 3.0020000772221707 13 40 3.2612413341916495 14 1 0.6552160063188204 14 2 4.266128361884828 14 3 10.909304211731216 14 4 -6.576410029627606 14 5 9.3818161719683 14 6 13.22790932645282 14 7 8.459015105856391 14 8 10.52553323504587 14 9 -0.6767588864378453 14 10 3.7101602332704964 14 11 -0.6117732127450672 14 12 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119 25 -2.582101355919132 119 26 -4.914726046410021 119 27 -1.401622547471101 119 28 -4.881093777797042 119 29 0.13538582109097952 119 30 0.01898516963358432 119 31 0.735889434064557 119 32 2.026775279844175 119 33 2.337309823456059 119 34 2.583704109918733 119 35 2.995342139613199 119 36 -2.613827522057276 119 37 -2.6733723915098615 119 38 -2.286121290999679 119 39 -2.29799992277783 119 40 -2.172091999141684 120 1 12.65521600631882 120 2 -1.733871638115172 120 3 13.909304211731216 120 4 10.423589970372394 120 5 16.3818161719683 120 6 15.22790932645282 120 7 1.4590151058563912 120 8 15.52553323504587 120 9 17.323241113562155 120 10 13.710160233270496 120 11 7.388226787254933 120 12 -3.5284493082821093 120 13 14.777508092772806 120 14 14.213000496553228 120 15 14.71711777793583 120 16 1.125216323362281 120 17 -8.32963851767829 120 18 14.84025926389759 120 19 16.851765982334303 120 20 -12.509459113353579 120 21 -10.827823189785708 120 22 -9.899515178516538 120 23 -9.666537547092126 120 24 -9.274211061340672 120 25 -8.182101355919132 120 26 -6.51472604641002 120 27 -6.6016225474711 120 28 -6.081093777797042 120 29 -5.664614178909021 120 30 -5.181014830366415 120 31 -4.064110565935444 120 32 -3.7732247201558256 120 33 -3.4626901765439415 120 34 -3.216295890081268 120 35 -2.8046578603868015 120 36 -2.613827522057276 120 37 -2.6733723915098615 120 38 -2.286121290999679 120 39 -2.29799992277783 120 40 -2.172091999141684 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 3762.0 5 142.0 6 273.0 7 206.0 8 201.0 9 370.0 10 591.0 11 726.0 12 817.0 13 906.0 14 936.0 15 1043.0 16 1034.0 17 1069.0 18 1088.0 19 1059.0 20 897.0 21 819.0 22 764.0 23 707.0 24 565.0 25 451.0 26 342.0 27 251.0 28 178.0 29 117.0 30 50.0 31 23.0 32 11.0 33 1.0 34 1.0 35 1.0 36 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 0.0 100.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.45814841572917 0.22706161626586852 0.1857776860357106 0.12901228196924347 8 97.5656299963897 0.6859559544071381 0.6550105730053123 1.0934034761978442 9 96.58322580645161 1.5896774193548389 0.7948387096774194 1.032258064516129 10 34.36420803051389 38.78666048141849 16.993969383021494 9.855162105046132 11 25.404597464178952 35.7849706215854 23.62643026492114 15.184001649314505 12 30.015463917525775 14.097938144329897 25.329896907216494 30.556701030927837 13 22.15750953509947 16.12720338109473 29.94536645706628 31.769920626739513 14 24.028450675188125 22.147201319451604 34.3108957839398 19.513452221420472 15 29.744356251932793 27.053911967838367 25.05927223997526 18.142459540253583 16 34.30058756829193 18.637253891351406 23.13679002164725 23.925368518709412 17 30.605092258530046 20.080404082053395 30.445314915988043 18.869188743428513 18 29.11555509741264 19.497989897948663 31.13081125657149 20.255643748067207 19 29.49811340259472 14.736134801261178 19.155424613635187 36.61032718250892 20 21.039068137305435 31.671992578084733 26.115864343882073 21.17307494072776 21 19.291825584991237 24.193382125554066 34.15111844139779 22.3636738480569 22 17.029172250283477 17.797134316049892 24.38923822286362 40.78445521080301 23 24.064529429955677 19.065044840738068 26.048860942170908 30.821564787135348 24 19.00319554685084 20.941140088650652 31.26997216781775 28.785692196680756 25 29.353773097966346 24.713533601734284 21.95210075358728 23.98059254671209 26 17.62025578625796 20.224719101123593 24.051157251592194 38.10386786102625 27 17.384131566737125 20.724854358921483 25.94731143991339 35.94370263442801 28 20.739251469223632 23.646767708011136 24.889163831322815 30.724816991442417 29 25.556071631315476 19.37348402745523 29.24085255715539 25.8295917840739 30 18.693856479091377 15.937016004130097 40.03097573567372 25.338151781104802 31 17.280570336312444 25.143358991579273 29.91682595443509 27.659244717673193 32 28.649484536082475 15.025773195876289 31.252577319587626 25.072164948453608 33 22.288659793814432 16.943298969072167 29.13917525773196 31.628865979381445 34 14.848984640758683 25.45098443459437 32.64611895680858 27.053911967838367 35 18.525773195876287 19.690721649484537 39.05154639175258 22.7319587628866 36 14.422680412371133 24.701030927835053 35.00515463917526 25.87113402061856 37 14.932686852014237 18.677464280187756 38.94878011038325 27.44106875741476 38 25.842696629213485 18.224925265436553 31.728687764148027 24.203690341201938 39 20.61340206185567 19.2319587628866 28.881443298969074 31.27319587628866 40 15.513864550046385 22.863622306978662 37.28481599835069 24.337697144624265 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 1.0 9 9.0 10 17.0 11 29.0 12 41.0 13 41.0 14 57.0 15 73.0 16 98.0 17 123.0 18 123.0 19 138.5 20 154.0 21 193.0 22 232.0 23 232.0 24 254.0 25 276.0 26 287.5 27 299.0 28 299.0 29 388.0 30 477.0 31 548.0 32 619.0 33 619.0 34 685.0 35 751.0 36 828.5 37 906.0 38 906.0 39 986.0 40 1066.0 41 1069.0 42 1072.0 43 1072.0 44 1147.0 45 1222.0 46 1393.0 47 1564.0 48 1564.0 49 1819.0 50 2074.0 51 2068.5 52 2063.0 53 2063.0 54 1910.5 55 1758.0 56 1624.0 57 1490.0 58 1490.0 59 1309.5 60 1129.0 61 956.5 62 784.0 63 784.0 64 663.5 65 543.0 66 441.0 67 339.0 68 339.0 69 264.0 70 189.0 71 131.5 72 74.0 73 74.0 74 56.5 75 39.0 76 27.0 77 15.0 78 15.0 79 12.0 80 9.0 81 6.0 82 3.0 83 3.0 84 1.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.12369858777445625 8 0.0670034017111638 9 0.13916091124626329 10 0.005154107823935676 11 0.0 12 0.010308215647871353 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.28347593031646223 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.14431501907019895 26 0.45871559633027525 27 0.025770539119678384 28 0.020616431295742706 29 0.1288526955983919 30 0.16493145036594165 31 0.23193485207710546 32 0.010308215647871353 33 0.010308215647871353 34 0.0 35 0.010308215647871353 36 0.010308215647871353 37 0.07731161735903515 38 0.0 39 0.010308215647871353 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 19402.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.41892588392949 #Duplication Level Percentage of deduplicated Percentage of total 1 95.17504622174509 82.24925265436553 2 3.1311504741456435 5.411813215132461 3 0.7216556330888054 1.8709411400886506 4 0.2683843263553409 0.9277394083084218 5 0.17295878809566412 0.747345634470673 6 0.09542553825967674 0.494794351097825 7 0.08349734597721716 0.5051025667456963 8 0.03578457684737878 0.2473971755489125 9 0.01789228842368939 0.13916091124626329 >10 0.2624202302141111 4.0253582104937635 >50 0.011928192282459593 0.7679620657664158 >100 0.023856384564919186 2.613132666735388 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ACTTATGGGAATTGACGGGTACGCACAGTTCTGAGTCGGA 137 0.7061127718791878 No Hit ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT 132 0.6803422327595093 No Hit ACTTATGGGTACCTGGTTGATCCTGCCCGTAGCATATGCT 128 0.6597258014637666 No Hit ACTTATGGGTACCTGGTTGATCCTGCCCGTAGCCTATGCT 110 0.5669518606329245 No Hit ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCCTATGCT 86 0.44325327285846816 No Hit ACTTATGGGAATTGACGGGTACGCACCGTTCTGAGTCGGA 63 0.32470879290794763 No Hit ACTTATGGGTACCTGGTTGATCCTGCCCGTAGCCTCTGCT 49 0.25255128337284816 No Hit ACTTATGGGAATTGACGGGTACGCACAGTTCTGAGTCCGA 31 0.15977734254200598 No Hit ACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC 31 0.15977734254200598 No Hit ACTTATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG 31 0.15977734254200598 No Hit ACTTATGGGTACCTGGTTGATCCTGCCCGTCGCCTATGCT 28 0.14431501907019895 No Hit ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATTCT 24 0.12369858777445625 No Hit ACTTATGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCG 23 0.11854447995052056 No Hit ACTTATGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC 22 0.11339037212658488 No Hit ACTTATGGGAATTGACGGGTACGCACCGTTCTGAGTCCGA 22 0.11339037212658488 No Hit ACTTATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC 22 0.11339037212658488 No Hit ACTTATGGGAATTGACGGGTACGCACAGTTCTGCGTCGGA 20 0.10308215647871354 No Hit ACTTATGGGTACCTGGTTGATCCTGCCCGTAGCATCTGCT 20 0.10308215647871354 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.005154107823935676 0.0 0.0 0.0 0.0 15 0.005154107823935676 0.0 0.0 0.0 0.0 16 0.005154107823935676 0.0 0.0 0.0 0.0 17 0.005154107823935676 0.0 0.0 0.0 0.0 18 0.005154107823935676 0.0 0.0 0.0 0.0 19 0.010308215647871353 0.0 0.0 0.0 0.0 20 0.015462323471807031 0.0 0.0 0.0 0.0 21 0.015462323471807031 0.0 0.0 0.0 0.0 22 0.015462323471807031 0.0 0.0 0.0 0.0 23 0.015462323471807031 0.0 0.0 0.0 0.0 24 0.015462323471807031 0.0 0.0 0.0 0.0 25 0.015462323471807031 0.0 0.0 0.0 0.0 26 0.015462323471807031 0.0 0.0 0.0 0.0 27 0.025770539119678384 0.0 0.0 0.0 0.0 28 0.025770539119678384 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTATGGT 20 0.0026733307 34.124676 3 ATGGGTG 25 1.9136493E-4 34.124676 5 ATGGGTA 220 0.0 34.124676 5 TTATGCG 20 0.0026733307 34.124676 3 ATGGGCC 20 0.0026733307 34.124676 5 CCCAGTT 20 0.0026733307 34.124676 24 CAGTTCT 40 7.1535396E-8 34.124676 26 CCAGTAG 60 1.8189894E-12 34.124676 26 CCTGCCA 80 0.0 34.124676 22 TAGCTCC 20 0.0026733307 34.124676 23 CCGTTCT 35 9.905343E-7 34.124676 26 GCCAGTC 20 0.0026733307 34.124676 25 TGGGGTT 30 1.3747098E-5 34.124676 6 TGGGGTG 75 0.0 34.124676 6 TGGGGTA 35 9.905343E-7 34.124676 6 TGGGGGC 60 1.8189894E-12 34.124676 6 TGGGGCT 35 9.905343E-7 34.124676 6 TGGGGAC 30 1.3747098E-5 34.124676 6 TGGGGAA 50 3.7471182E-10 34.124676 6 TGCCAGT 80 0.0 34.124676 24 >>END_MODULE