FastQCFastQC Report
Fri 17 Jun 2016
SRR1526019_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526019_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4845
Sequences flagged as poor quality0
Sequence length40
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT691.4241486068111455No Hit
ACTTATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA681.4035087719298245No Hit
ACTTATGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT541.1145510835913313No Hit
ACTTATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.7636738906088751No Hit
ACTTATGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG210.43343653250773995No Hit
ACTTATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA180.37151702786377705No Hit
ACTTATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.24767801857585142No Hit
ACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC110.22703818369453044No Hit
ACTTATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.20639834881320948No Hit
ACTTATGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT80.1651186790505676No Hit
ACTTATGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATT70.14447884416924664No Hit
ACTTATGGGGCCCACTTCGCCATCATATTCGTAGGAGTAA70.14447884416924664No Hit
ACTTATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG70.14447884416924664No Hit
ACTTATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTT70.14447884416924664No Hit
ACTTATGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.12383900928792571No Hit
ACTTATGGGATCCTCATTGTACTGGAGGAGCAGCTGGCTA60.12383900928792571No Hit
ACTTATGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG60.12383900928792571No Hit
ACTTATGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT60.12383900928792571No Hit
ACTTATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAA60.12383900928792571No Hit
ACTTATGGGGGGGGGTCTGGTGTTCACCCTGTTTCTCATA60.12383900928792571No Hit
ACTTATGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGG50.10319917440660474No Hit
ACTTATGGAGAAGTGATTTTCCACAGCTGTGGTTCAGGTA50.10319917440660474No Hit
ACTTATGGGGCAGTGCTACTTATGGGATCATTGGCTATGC50.10319917440660474No Hit
ACTTATGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.10319917440660474No Hit
ACTTATGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTT50.10319917440660474No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTA200.002444718534.210535
ATGGGAT405.279071E-834.210535
GAGTTTT200.002444718534.2105331
TGGGTCA405.279071E-834.210536
AGTTTTT200.002444718534.2105332
TGAGTTT200.002444718534.2105330
ATGGGTC453.5452103E-934.2105265
ATGGGGG950.034.2105265
ATGGGAG357.8084304E-734.2105265
TGGGGGG601.8189894E-1234.2105266
TGGGGCC251.6790826E-434.2105266
TGGGGAG251.6790826E-434.2105266
TATGGGC301.1480053E-534.2105264
ATCCTCT358.502011E-733.85416816
CTGAGAT200.002576405433.85416824
CTCTTCT358.502011E-733.85416819
GTTTTTG200.002576405433.85416833
TTCTGAA200.002576405433.85416822
CTTCTGA358.502011E-733.85416821
TATGGGG2500.032.1578944
Produced by FastQC (version 0.11.5)