##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526019_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4845 Sequences flagged as poor quality 0 Sequence length 40 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.061300309597524 26.0 4.0 30.0 4.0 33.0 2 14.801238390092879 13.0 4.0 25.0 4.0 31.0 3 14.408255933952528 13.0 4.0 24.0 4.0 31.0 4 14.606604747162022 13.0 4.0 24.0 4.0 30.0 5 14.078844169246645 11.0 4.0 24.0 4.0 31.0 6 13.074097007223942 8.0 4.0 22.0 4.0 29.0 7 15.68482972136223 12.0 4.0 27.0 4.0 33.0 8 15.986377708978328 14.0 4.0 28.0 4.0 33.0 9 15.5453044375645 9.0 4.0 27.0 4.0 33.0 10 13.936842105263159 4.0 4.0 25.0 4.0 32.0 11 14.42765737874097 4.0 4.0 27.0 4.0 34.0 12 13.924458204334366 4.0 4.0 26.0 4.0 33.0 13 12.996904024767803 4.0 4.0 24.0 4.0 32.0 14 13.627244582043344 4.0 4.0 26.0 4.0 33.0 15 13.137048503611972 4.0 4.0 25.0 4.0 33.0 16 11.219195046439628 4.0 4.0 20.0 4.0 30.0 17 10.602063983488133 4.0 4.0 17.0 4.0 29.0 18 10.231166150670795 4.0 4.0 16.0 4.0 28.0 19 9.89329205366357 4.0 4.0 14.0 4.0 27.0 20 10.557275541795665 4.0 4.0 17.0 4.0 29.0 21 9.281733746130032 4.0 4.0 13.0 4.0 26.0 22 9.20970072239422 4.0 4.0 12.0 4.0 26.0 23 9.194427244582043 4.0 4.0 12.0 4.0 26.0 24 8.919711042311661 4.0 4.0 11.0 4.0 25.0 25 9.3781217750258 4.0 4.0 13.0 4.0 26.0 26 9.621671826625388 4.0 4.0 14.0 4.0 27.0 27 9.907739938080494 4.0 4.0 14.0 4.0 29.0 28 10.217956656346749 4.0 4.0 16.0 4.0 30.0 29 9.249122807017544 4.0 4.0 12.0 4.0 27.0 30 9.398555211558307 4.0 4.0 13.0 4.0 27.0 31 10.634055727554179 4.0 4.0 17.0 4.0 32.0 32 10.539112487100104 4.0 4.0 16.0 4.0 31.0 33 10.359752321981425 4.0 4.0 16.0 4.0 31.0 34 10.448503611971104 4.0 4.0 15.0 4.0 32.0 35 10.404540763673891 4.0 4.0 13.0 4.0 32.0 36 10.356656346749226 4.0 4.0 4.0 4.0 33.0 37 10.197729618163054 4.0 4.0 4.0 4.0 33.0 38 9.924458204334366 4.0 4.0 4.0 4.0 33.0 39 9.762848297213623 4.0 4.0 4.0 4.0 32.0 40 9.795872033023736 4.0 4.0 4.0 4.0 32.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 10.552480608524572 1 2 3.0238813689363155 1 3 3.4977425504898 1 4 -1.5180741419752426 1 5 1.947582728626685 1 6 5.066747324714358 1 7 2.26220052840932 1 8 -5.776547994130407 1 9 -7.2833076264394965 1 10 1.8009894257147003 1 11 1.7208216996678551 1 12 4.278781200484495 1 13 0.32668308492484677 1 14 0.9983354862475746 1 15 6.9521935329627595 1 16 -2.239076857208726 1 17 -3.9009242283967556 1 18 1.491075469097444 1 19 -1.4435415317283429 1 20 -5.195708320983046 1 21 -0.5354131521713956 1 22 -0.4097762737872621 1 23 -0.6694614299009913 1 24 4.014760178771167 1 25 -3.85490678430239 1 26 -4.344943975438479 1 27 -4.630717603519802 1 28 -4.84829157411575 1 29 -3.96870388463795 1 30 -3.9575899070404548 1 31 -5.1710242138813545 1 32 -4.8142394540746185 1 33 -4.623445784984245 1 34 -4.838429610682359 1 35 -5.010121715890946 1 36 -4.783921786394311 1 37 -4.553058815421451 1 38 -4.553804193364632 1 39 -4.180188126891425 1 40 -4.236828312103038 2 1 -4.697519391475428 2 2 -0.7261186310636845 2 3 -4.3772574495102 2 4 -4.768074141975243 2 5 -7.927417271373315 2 6 -2.308252675285642 2 7 -3.23779947159068 2 8 -4.151547994130407 2 9 -8.658307626439496 2 10 -2.4490105742852997 2 11 -5.279178300332145 2 12 0.15378120048449517 2 13 0.5766830849248468 2 14 -0.12666451375242538 2 15 0.32719353296275955 2 16 -2.739076857208726 2 17 -0.7759242283967556 2 18 0.6160754690974439 2 19 -0.06854153172834287 2 20 3.929291679016954 2 21 1.5895868478286044 2 22 -0.4097762737872621 2 23 2.9555385700990087 2 24 -2.1102398212288334 2 25 2.77009321569761 2 26 3.280056024561521 2 27 2.994282396480198 2 28 4.15170842588425 2 29 1.90629611536205 2 30 2.7924100929595452 2 31 3.7039757861186455 2 32 3.5607605459253815 2 33 3.251554215015755 2 34 2.1615703893176406 2 35 1.7398782841090537 2 36 2.341078213605689 2 37 6.071941184578549 2 38 1.1961958066353677 2 39 2.0698118731085753 2 40 0.013171687896962325 4 1 13.802480608524572 4 2 18.023881368936316 4 3 19.4977425504898 4 4 12.731925858024757 4 5 18.197582728626685 4 6 15.066747324714358 4 7 18.51220052840932 4 8 16.223452005869593 4 9 15.466692373560504 4 10 10.0509894257147 4 11 16.720821699667855 4 12 4.528781200484495 4 13 7.576683084924847 4 14 5.998335486247575 4 15 5.4521935329627595 4 16 -0.23907685720872607 4 17 6.099075771603244 4 18 -1.258924530902556 4 19 8.556458468271657 4 20 -5.445708320983046 4 21 -4.035413152171396 4 22 -3.909776273787262 4 23 -4.169461429900991 4 24 -3.2352398212288334 4 25 -3.85490678430239 4 26 -4.344943975438479 4 27 -4.630717603519802 4 28 -4.84829157411575 4 29 -3.96870388463795 4 30 -3.9575899070404548 4 31 -5.1710242138813545 4 32 -4.8142394540746185 4 33 -4.623445784984245 4 34 -4.838429610682359 4 35 -5.010121715890946 4 36 -4.783921786394311 4 37 -4.553058815421451 4 38 -4.553804193364632 4 39 -4.180188126891425 4 40 -4.236828312103038 5 1 8.302480608524572 5 2 13.023881368936316 5 3 12.4977425504898 5 4 7.731925858024757 5 5 1.697582728626685 5 6 14.066747324714358 5 7 -5.48779947159068 5 8 7.723452005869593 5 9 16.466692373560505 5 10 17.5509894257147 5 11 21.720821699667855 5 12 17.028781200484495 5 13 16.076683084924845 5 14 4.498335486247575 5 15 22.95219353296276 5 16 -5.739076857208726 5 17 -5.400924228396756 5 18 -5.258924530902556 5 19 -4.943541531728343 5 20 -5.445708320983046 5 21 -4.035413152171396 5 22 -3.909776273787262 5 23 -4.169461429900991 5 24 -3.2352398212288334 5 25 -3.85490678430239 5 26 -4.344943975438479 5 27 -4.630717603519802 5 28 -4.84829157411575 5 29 -3.96870388463795 5 30 -3.9575899070404548 5 31 -5.1710242138813545 5 32 -4.8142394540746185 5 33 -4.623445784984245 5 34 -4.838429610682359 5 35 -5.010121715890946 5 36 -4.783921786394311 5 37 -4.553058815421451 5 38 -4.553804193364632 5 39 -4.180188126891425 5 40 -4.236828312103038 6 1 6.102480608524573 6 2 -1.8761186310636848 6 3 4.0977425504898015 6 4 0.33192585802475705 6 5 5.5975827286266835 6 6 -4.233252675285643 6 7 0.7122005284093191 6 8 6.0234520058695935 6 9 7.4666923735605035 6 10 0.15098942571469998 6 11 2.4208216996678544 6 12 5.328781200484496 6 13 5.676683084924848 6 14 6.498335486247575 6 15 9.352193532962758 6 16 7.760923142791274 6 17 12.999075771603243 6 18 5.441075469097443 6 19 6.056458468271657 6 20 9.054291679016954 6 21 8.764586847828605 6 22 7.790223726212737 6 23 9.230538570099007 6 24 6.864760178771166 6 25 6.345093215697609 6 26 8.755056024561522 6 27 9.569282396480197 6 28 10.351708425884249 6 29 9.33129611536205 6 30 10.742410092959545 6 31 8.328975786118646 6 32 8.785760545925383 6 33 10.876554215015755 6 34 10.66157038931764 6 35 11.489878284109054 6 36 11.51607821360569 6 37 13.646941184578548 6 38 11.846195806635366 6 39 10.419811873108577 6 40 14.163171687896961 7 1 7.052480608524572 7 2 13.023881368936316 7 3 -0.5022574495101999 7 4 10.981925858024757 7 5 1.072582728626685 7 6 -3.558252675285642 7 7 -2.61279947159068 7 8 8.848452005869593 7 9 11.216692373560504 7 10 9.5509894257147 7 11 1.9708216996678551 7 12 13.778781200484495 7 13 13.826683084924847 7 14 5.373335486247575 7 15 9.70219353296276 7 16 16.635923142791274 7 17 14.474075771603244 7 18 15.491075469097444 7 19 11.806458468271657 7 20 12.929291679016954 7 21 12.964586847828604 7 22 15.465223726212738 7 23 15.455538570099009 7 24 4.014760178771167 7 25 13.77009321569761 7 26 12.530056024561521 7 27 13.869282396480198 7 28 10.27670842588425 7 29 11.15629611536205 7 30 13.292410092959546 7 31 17.203975786118647 7 32 15.935760545925381 7 33 13.626554215015755 7 34 15.28657038931764 7 35 18.489878284109054 7 36 17.46607821360569 7 37 16.94694118457855 7 38 14.821195806635368 7 39 18.319811873108577 7 40 12.638171687896962 8 1 7.635813941857904 8 2 8.273881368936316 8 3 8.2477425504898 8 4 7.0652591913580896 8 5 0.6975827286266849 8 6 7.233413991381026 8 7 2.01220052840932 8 8 2.973452005869593 8 9 5.7166923735605035 8 10 -0.11567724095196574 8 11 -2.195844966998811 8 12 1.2787812004844952 8 13 -1.2566502484084872 8 14 0.5816688195809085 8 15 1.2021935329627595 8 16 3.42758980945794 8 17 2.0990757716032444 8 18 -2.008924530902556 8 19 -3.776874865061676 8 20 -1.3623749876497122 8 21 -1.2020798188380626 8 22 -0.3264429404539291 8 23 0.1638719034323426 8 24 -2.4019064878955003 8 25 -0.6882401176357229 8 26 1.155056024561521 8 27 3.452615729813532 8 28 0.9017084258842498 8 29 1.03129611536205 8 30 0.6257434262928792 8 31 3.1623091194519795 8 32 4.352427212592048 8 33 3.709887548349089 8 34 3.5782370559843066 8 35 3.3232116174423876 8 36 3.049411546939023 8 37 2.8636078512452148 8 38 2.862862473302034 8 39 3.4864785397752414 8 40 5.763171687896962 9 1 7.802480608524572 9 2 18.523881368936316 9 3 21.4977425504898 9 4 18.73192585802476 9 5 21.697582728626685 9 6 24.566747324714356 9 7 20.01220052840932 9 8 21.723452005869593 9 9 20.466692373560505 9 10 23.5509894257147 9 11 23.720821699667855 9 12 17.028781200484495 9 13 23.576683084924845 9 14 21.498335486247576 9 15 24.95219353296276 9 16 17.260923142791274 9 17 19.099075771603246 9 18 23.741075469097446 9 19 8.556458468271657 9 20 10.054291679016954 9 21 11.464586847828604 9 22 8.590223726212738 9 23 6.830538570099009 9 24 12.264760178771166 9 25 10.64509321569761 9 26 12.655056024561521 9 27 10.869282396480198 9 28 12.15170842588425 9 29 11.53129611536205 9 30 13.042410092959546 9 31 10.328975786118646 9 32 9.185760545925381 9 33 12.376554215015755 9 34 12.16157038931764 9 35 11.989878284109054 9 36 -4.783921786394311 9 37 -4.553058815421451 9 38 -4.553804193364632 9 39 -4.180188126891425 9 40 -4.236828312103038 11 1 5.802480608524572 11 2 8.523881368936316 11 3 3.9977425504898 11 4 10.231925858024757 11 5 10.447582728626685 11 6 3.566747324714358 11 7 9.76220052840932 11 8 10.223452005869593 11 9 9.966692373560504 11 10 11.0509894257147 11 11 7.720821699667855 11 12 9.528781200484495 11 13 1.5766830849248468 11 14 9.498335486247575 11 15 7.2021935329627595 11 16 10.010923142791274 11 17 7.599075771603244 11 18 5.741075469097444 11 19 11.056458468271657 11 20 9.554291679016954 11 21 14.464586847828604 11 22 17.090223726212738 11 23 15.330538570099009 11 24 11.014760178771166 11 25 15.39509321569761 11 26 21.40505602456152 11 27 16.369282396480198 11 28 20.65170842588425 11 29 19.28129611536205 11 30 15.292410092959546 11 31 18.578975786118647 11 32 14.935760545925381 11 33 17.126554215015755 11 34 8.91157038931764 11 35 9.989878284109054 11 36 11.46607821360569 11 37 11.696941184578549 11 38 11.696195806635368 11 39 12.319811873108575 11 40 12.263171687896962 12 1 4.15962346566743 12 2 4.452452797507743 12 3 -4.216543163795913 12 4 -2.2680741419752426 12 5 4.697582728626685 12 6 -0.21896696099992852 12 7 9.797914814123605 12 8 9.15202343444102 12 9 9.180978087846219 12 10 9.836703711428985 12 11 10.00653598538214 12 12 11.171638343341638 12 13 9.790968799210562 12 14 8.498335486247575 12 15 8.666479247248475 12 16 8.118065999934132 12 17 10.027647200174671 12 18 8.026789754811729 12 19 8.62788703970023 12 20 10.26857739330267 12 21 15.107443990685747 12 22 8.090223726212738 12 23 13.401967141527582 12 24 9.336188750199739 12 25 10.14509321569761 12 26 16.226484595990094 12 27 17.369282396480198 12 28 14.865994140169965 12 29 13.602724686790623 12 30 11.042410092959546 12 31 11.828975786118646 12 32 10.328617688782524 12 33 6.6622685007300415 12 34 5.161570389317641 12 35 7.704163998394769 12 36 10.073221070748547 12 37 8.589798327435691 12 38 2.8747672352067966 12 39 9.819811873108575 12 40 7.191743116468391 13 1 2.96914727519124 13 2 5.2738813689363155 13 3 4.414409217156468 13 4 0.14859252469142348 13 5 -1.9690839380399812 13 6 -1.933252675285642 13 7 6.678867195075988 13 8 8.39011867253626 13 9 6.7166923735605035 13 10 3.717656092381368 13 11 3.970821699667855 13 12 4.862114533817827 13 13 5.076683084924847 13 14 2.4150021529142407 13 15 2.3688601996294256 13 16 4.67758980945794 13 17 5.2657424382699105 13 18 5.574408802430778 13 19 4.056458468271657 13 20 4.804291679016954 13 21 5.1312535144952705 13 22 5.673557059546072 13 23 7.080538570099009 13 24 9.764760178771166 13 25 8.478426549030942 13 26 8.405056024561521 13 27 8.95261572981353 13 28 10.235041759217582 13 29 9.364629448695382 13 30 7.459076759626211 13 31 10.245642452785313 13 32 12.269093879258714 13 33 9.959887548349087 13 34 7.161570389317641 13 35 10.323211617442386 13 36 9.049411546939021 13 37 8.946941184578549 13 38 7.029529139968702 13 39 9.319811873108575 13 40 8.596505021230294 14 1 11.302480608524572 14 2 18.023881368936316 14 3 -11.5022574495102 14 4 -11.268074141975243 14 5 -11.302417271373315 14 6 -8.933252675285642 14 7 -11.48779947159068 14 8 -11.276547994130407 14 9 -10.533307626439496 14 10 -9.4490105742853 14 11 -9.279178300332145 14 12 -7.971218799515505 14 13 -7.923316915075153 14 14 -8.501664513752425 14 15 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3.7855268662960935 15 16 0.7609231427912739 15 17 -1.7342575617300886 15 18 -2.758924530902556 15 19 1.389791801604991 15 20 0.5542916790169539 15 21 -2.5354131521713956 15 22 3.923557059546072 15 23 0.9972052367656747 15 24 -0.7352398212288334 15 25 -0.8549067843023899 15 26 -1.1782773087718121 15 27 2.702615729813532 15 28 -0.6816249074490841 15 29 0.8646294486953838 15 30 0.5424100929595452 15 31 2.9956424527853116 15 32 3.3524272125920476 15 33 5.043220881682421 15 34 4.4949037226509745 15 35 4.65654495077572 15 36 0.3827448802723552 15 37 0.6136078512452148 15 38 0.6128624733020338 15 39 0.9864785397752414 15 40 -4.236828312103038 16 1 5.4563267623707254 16 2 11.716189061244009 16 3 9.113127165874417 16 4 10.578079704178604 16 5 -1.1485711175271618 16 6 10.451362709329741 16 7 8.74296975917855 16 8 12.184990467408053 16 9 12.005153912022044 16 10 13.08945096417624 16 11 15.182360161206315 16 12 18.490319662022955 16 13 11.922836931078693 16 14 14.190643178555268 16 15 16.644501225270453 16 16 16.799384681252814 16 17 14.445229617757091 16 18 10.81799854602052 16 19 12.902612314425504 16 20 16.092753217478496 16 21 13.810740693982451 16 22 11.551762187751198 16 23 13.292077031637469 16 24 13.303221717232706 16 25 15.760477831082227 16 26 19.42428679379229 16 27 18.06159008878789 16 28 16.767093041268865 16 29 13.26206534613128 16 30 17.96548701603647 16 31 17.828975786118647 16 32 17.262683622848456 16 33 13.991938830400372 16 34 15.776955004702257 16 35 13.29757059180136 16 36 12.062232059759536 16 37 12.677710415347779 16 38 11.984657345096908 16 39 9.358273411570115 16 40 9.301633226358502 17 1 6.413591719635683 17 2 -6.9761186310636845 17 3 -3.391146338399089 17 4 6.620814746913647 17 5 4.253138284182242 17 6 2.1778584358254687 17 7 5.956644972853763 17 8 5.27900756142515 17 9 4.355581262449393 17 10 6.217656092381368 17 11 9.276377255223412 17 12 10.584336756040052 17 13 6.410016418258179 17 14 11.276113264025353 17 15 7.285526866296092 17 16 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-4.236828312103038 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 2012.0 5 70.0 6 111.0 7 123.0 8 112.0 9 134.0 10 177.0 11 204.0 12 146.0 13 111.0 14 87.0 15 67.0 16 61.0 17 47.0 18 42.0 19 59.0 20 118.0 21 173.0 22 219.0 23 192.0 24 166.0 25 143.0 26 113.0 27 65.0 28 39.0 29 30.0 30 13.0 31 4.0 32 6.0 33 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 0.0 100.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.02832334091379 0.4548273723382261 0.3514575149886293 0.16539177175935496 8 97.68595041322314 1.0743801652892562 0.7024793388429752 0.5371900826446281 9 96.35912287960281 1.4273893256102608 1.2412081092263136 0.9722796855606123 10 42.37043155069172 35.01961594053273 13.71051001445385 8.899442494321702 11 27.317778236630186 29.878174685112533 25.1909973157134 17.613049762543877 12 27.925696594427247 28.81320949432404 23.24045407636739 20.020639834881322 13 21.09940070262451 28.931597437487085 25.935110560033063 24.03389129985534 14 21.98596201486375 33.98018166804294 27.43600330305533 16.597853014037987 15 29.040247678018577 29.78328173374613 25.304437564499484 15.87203302373581 16 24.912280701754387 35.14963880288958 26.08875128998968 13.849329205366356 17 23.1420313790256 29.95458298926507 31.069364161849713 15.83402146985962 18 23.410404624277454 31.44095788604459 26.89925681255161 18.249380677126343 19 21.8091697645601 33.39529120198265 24.86575795125981 19.92978108219744 20 21.135190918472652 33.271413828689376 29.18472652218782 16.408668730650156 21 19.463364293085654 31.351909184726523 30.58823529411765 18.596491228070175 22 21.197110423116616 29.267285861713106 29.432404540763674 20.103199174406605 23 18.191203799297956 31.798472021474293 30.31179021267809 19.698533966549657 24 19.050567595459235 32.074303405572756 28.441692466460267 20.43343653250774 25 22.14182344428365 30.390738060781473 28.736820343187926 18.73061815174695 26 25.599669148056243 33.043837882547564 23.01488833746898 18.341604631927215 27 19.701368726669433 35.81501451679801 23.683119037743676 20.800497718788886 28 21.7606943583385 35.77185368877867 24.11655300681959 18.350898946063236 29 25.497512437810943 32.83582089552239 23.445273631840795 18.22139303482587 30 21.453644435267396 33.305802188726 28.990295271525913 16.250258104480693 31 24.566473988439306 34.62014863748968 24.15359207266722 16.659785301403797 32 25.33112582781457 33.319536423841065 25.43460264900662 15.914735099337749 33 23.50877192982456 33.106295149638804 24.705882352941178 18.67905056759546 34 20.169281585466557 33.07184145334434 30.573905862923205 16.184971098265898 35 19.174406604747162 32.26006191950464 32.87925696594427 15.686274509803921 36 19.292803970223325 34.34656741108354 27.1712158808933 19.189412737799834 37 20.990712074303406 32.36326109391125 30.092879256965944 16.553147574819402 38 23.0546955624355 31.06295149638803 29.060887512899896 16.821465428276575 39 21.238390092879257 33.9938080495356 24.99484004127967 19.77296181630547 40 18.617131062951497 33.62229102167183 30.42311661506708 17.3374613003096 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 1.0 5 2.0 6 5.0 7 8.0 8 8.0 9 55.0 10 102.0 11 99.5 12 97.0 13 97.0 14 86.5 15 76.0 16 70.0 17 64.0 18 64.0 19 63.0 20 62.0 21 73.0 22 84.0 23 84.0 24 86.5 25 89.0 26 105.5 27 122.0 28 122.0 29 146.0 30 170.0 31 193.5 32 217.0 33 217.0 34 238.5 35 260.0 36 297.0 37 334.0 38 334.0 39 387.5 40 441.0 41 406.0 42 371.0 43 371.0 44 406.0 45 441.0 46 473.5 47 506.0 48 506.0 49 443.0 50 380.0 51 340.5 52 301.0 53 301.0 54 295.0 55 289.0 56 252.5 57 216.0 58 216.0 59 159.5 60 103.0 61 81.5 62 60.0 63 60.0 64 45.0 65 30.0 66 19.5 67 9.0 68 9.0 69 9.5 70 10.0 71 5.5 72 1.0 73 1.0 74 0.5 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.1651186790505676 8 0.10319917440660474 9 0.22703818369453044 10 0.0412796697626419 11 0.0412796697626419 12 0.0 13 0.12383900928792571 14 0.02063983488132095 15 0.0 16 0.0 17 0.02063983488132095 18 0.02063983488132095 19 0.061919504643962855 20 0.0 21 0.0 22 0.0 23 0.0412796697626419 24 0.0 25 0.1651186790505676 26 0.18575851393188852 27 0.4747162022703818 28 0.12383900928792571 29 0.43343653250773995 30 0.0412796697626419 31 0.02063983488132095 32 0.26831785345717235 33 0.0 34 0.02063983488132095 35 0.0 36 0.18575851393188852 37 0.0 38 0.0 39 0.0 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 4845.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.76986584107327 #Duplication Level Percentage of deduplicated Percentage of total 1 94.2197906755471 81.75438596491227 2 4.234062797335871 7.347781217750258 3 0.6898192197906755 1.7956656346749225 4 0.26165556612749763 0.9081527347781218 5 0.11893434823977164 0.5159958720330238 6 0.142721217887726 0.7430340557275541 7 0.09514747859181732 0.5779153766769866 8 0.02378686964795433 0.1651186790505676 9 0.0 0.0 >10 0.142721217887726 2.2497420020639836 >50 0.071360608943863 3.9422084623323013 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT 69 1.4241486068111455 No Hit ACTTATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 68 1.4035087719298245 No Hit ACTTATGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT 54 1.1145510835913313 No Hit ACTTATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 37 0.7636738906088751 No Hit ACTTATGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG 21 0.43343653250773995 No Hit ACTTATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA 18 0.37151702786377705 No Hit ACTTATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12 0.24767801857585142 No Hit ACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC 11 0.22703818369453044 No Hit ACTTATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10 0.20639834881320948 No Hit ACTTATGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT 8 0.1651186790505676 No Hit ACTTATGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATT 7 0.14447884416924664 No Hit ACTTATGGGGCCCACTTCGCCATCATATTCGTAGGAGTAA 7 0.14447884416924664 No Hit ACTTATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG 7 0.14447884416924664 No Hit ACTTATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTT 7 0.14447884416924664 No Hit ACTTATGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6 0.12383900928792571 No Hit ACTTATGGGATCCTCATTGTACTGGAGGAGCAGCTGGCTA 6 0.12383900928792571 No Hit ACTTATGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG 6 0.12383900928792571 No Hit ACTTATGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT 6 0.12383900928792571 No Hit ACTTATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAA 6 0.12383900928792571 No Hit ACTTATGGGGGGGGGTCTGGTGTTCACCCTGTTTCTCATA 6 0.12383900928792571 No Hit ACTTATGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGG 5 0.10319917440660474 No Hit ACTTATGGAGAAGTGATTTTCCACAGCTGTGGTTCAGGTA 5 0.10319917440660474 No Hit ACTTATGGGGCAGTGCTACTTATGGGATCATTGGCTATGC 5 0.10319917440660474 No Hit ACTTATGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5 0.10319917440660474 No Hit ACTTATGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTT 5 0.10319917440660474 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.02063983488132095 0.0 0.0 0.0 0.0 12 0.02063983488132095 0.0 0.0 0.0 0.0 13 0.02063983488132095 0.0 0.0 0.0 0.0 14 0.02063983488132095 0.0 0.0 0.0 0.0 15 0.02063983488132095 0.0 0.0 0.0 0.0 16 0.02063983488132095 0.0 0.0 0.0 0.0 17 0.02063983488132095 0.0 0.0 0.0 0.0 18 0.02063983488132095 0.0 0.0 0.0 0.0 19 0.02063983488132095 0.0 0.0 0.0 0.0 20 0.02063983488132095 0.0 0.0 0.0 0.0 21 0.02063983488132095 0.0 0.0 0.0 0.0 22 0.02063983488132095 0.0 0.0 0.0 0.0 23 0.0412796697626419 0.0 0.0 0.0 0.0 24 0.0412796697626419 0.0 0.0 0.0 0.0 25 0.0412796697626419 0.0 0.0 0.0 0.0 26 0.0412796697626419 0.0 0.0 0.0 0.0 27 0.0412796697626419 0.0 0.0 0.0 0.0 28 0.06191950464396285 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGGTA 20 0.0024447185 34.21053 5 ATGGGAT 40 5.279071E-8 34.21053 5 GAGTTTT 20 0.0024447185 34.21053 31 TGGGTCA 40 5.279071E-8 34.21053 6 AGTTTTT 20 0.0024447185 34.21053 32 TGAGTTT 20 0.0024447185 34.21053 30 ATGGGTC 45 3.5452103E-9 34.210526 5 ATGGGGG 95 0.0 34.210526 5 ATGGGAG 35 7.8084304E-7 34.210526 5 TGGGGGG 60 1.8189894E-12 34.210526 6 TGGGGCC 25 1.6790826E-4 34.210526 6 TGGGGAG 25 1.6790826E-4 34.210526 6 TATGGGC 30 1.1480053E-5 34.210526 4 ATCCTCT 35 8.502011E-7 33.854168 16 CTGAGAT 20 0.0025764054 33.854168 24 CTCTTCT 35 8.502011E-7 33.854168 19 GTTTTTG 20 0.0025764054 33.854168 33 TTCTGAA 20 0.0025764054 33.854168 22 CTTCTGA 35 8.502011E-7 33.854168 21 TATGGGG 250 0.0 32.157894 4 >>END_MODULE