FastQCFastQC Report
Fri 17 Jun 2016
SRR1526018_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526018_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22810
Sequences flagged as poor quality0
Sequence length56
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG5322.332310390179746No Hit
CATGATGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT2381.0434020166593598No Hit
CATGATGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC2080.9118807540552389No Hit
CATGATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG1270.5567733450241122No Hit
CATGATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG780.34195528277071463No Hit
CATGATGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT740.32441911442349847No Hit
CATGATGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA670.29373081981587024No Hit
CATGATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG550.24112231477422183No Hit
CATGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA520.22797018851380974No Hit
CATGATGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC510.2235861464270057No Hit
CATGATGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT480.21043402016659357No Hit
CATGATGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC480.21043402016659357No Hit
CATGATGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT470.20604997807978953No Hit
CATGATGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGG470.20604997807978953No Hit
CATGATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.1841297676457694No Hit
CATGATGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA390.17097764138535732No Hit
CATGATGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC380.16659359929855327No Hit
CATGATGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT370.16220955721174923No Hit
CATGATGGGATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT360.1578255151249452No Hit
CATGATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG350.15344147303814115No Hit
CATGATGGGACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGG310.13590530469092504No Hit
CATGATGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG310.13590530469092504No Hit
CATGATGGGAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGA290.12713722051731696No Hit
CATGATGGGCATGTTACTTTATGCTGCTGGCTCTTGTACCTTGGGAGATTGTAACA280.12275317843051294No Hit
CATGATGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG270.1183691363437089No Hit
CATGATGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC250.10960105217010083No Hit
CATGATGGGAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGAT250.10960105217010083No Hit
CATGATGGGACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTT240.10521701008329679No Hit
CATGATGGGATTCGTAGGAGTAAACATAACATTCTTCCCTCAACATTTCCTGGGCC240.10521701008329679No Hit
CATGATGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC240.10521701008329679No Hit
CATGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA240.10521701008329679No Hit
CATGATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA240.10521701008329679No Hit
CATGATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.10083296799649277No Hit
CATGATGGGAACTGTGGTAATTCTAGAGCTAATACATGCCCACGGGCGCTGACCCC230.10083296799649277No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGTC204.0220926E-450.18625335
TATGCTT800.050.18625335
ATGGTAG150.00820345250.18625334
ATATGCT900.050.18625334
AATCCTC150.00827582250.0752215
TCGATGG150.00827582250.0752231
AGGATCG150.00827582250.0752232
TTCGATG150.00827582250.0752230
TGTTTGC150.00827582250.0752226
GATGAGG150.00827582250.0752228
AACTTTC150.00827582250.0752226
CTGGTTG950.050.0752213
ACCTGGT950.050.0752211
ACTTTCG150.00827582250.0752227
TAACATG150.00827582250.0752230
CGATGGT150.00827582250.0752232
CCTGGTT950.050.0752212
TTTCGAT150.00827582250.0752229
AACATGT150.00827582250.0752231
TCAACTT150.00834866449.96467624
Produced by FastQC (version 0.11.5)