FastQCFastQC Report
Fri 17 Jun 2016
SRR1526017_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526017_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3887
Sequences flagged as poor quality0
Sequence length40
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT380.9776177000257269No Hit
CATGATGGGTACCTGGTTGATCCTGCCCGTAGCATATGCT180.463082068433239No Hit
CATGATGGGTACCTGGTTGATCCTGCCAGTAGCCTATGCT160.41162850527399025No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG150.3859017236943658No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC140.36017494211474144No Hit
CATGATGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT110.2829945973758683No Hit
CATGATGGGTACCTGGTTGATCCTGCCCGTAGCCTATGCT110.2829945973758683No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAG80.20581425263699513No Hit
CATGATGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT70.18008747105737072No Hit
CATGATGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT70.18008747105737072No Hit
CATGATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCC50.12863390789812193No Hit
CATGATGGGTACCTGGTTCATCCTGCCAGTAGCATATGCT50.12863390789812193No Hit
CATGATGGGCTCGTCTTAATCAGCAACATCTTTTGATACA50.12863390789812193No Hit
CATGATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT40.10290712631849756No Hit
CATGATGGGTACCTGGTTGATCCTGCCAGTCGCATATGCT40.10290712631849756No Hit
CATGATGGGGGGCTGGTGCGATGGCTCAGTGGGTCAGAGC40.10290712631849756No Hit
CATGATGGGTACCTGGTTGATCCTGCCAGTAGCATCTGCT40.10290712631849756No Hit
CATGATGGGCAGAGTCGATTTTTTTTTTTTTTTTTTTTTT40.10290712631849756No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAG40.10290712631849756No Hit
CATGATGGGTACCTGGTTGATCCTGCCCGTCGCATATGCT40.10290712631849756No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGTAG251.4927189E-434.65333626
CCTGCCA251.4927189E-434.65333622
GCCAGTA251.4927189E-434.65333625
TGCCAGT251.4927189E-434.65333624
ATCCTGC404.25116E-834.6533320
TCCTGCC404.25116E-834.6533321
CAGTAGC251.6180001E-434.1973727
TAGCATA251.6180001E-434.1973730
AGCATAT251.6180001E-434.1973731
GTAGCAT251.6180001E-434.1973729
CATGATG3850.033.753251
ATGGGTA551.4551915E-1133.7532465
TGATGGG3750.033.7532463
GATGGGT650.033.7532464
GATGGGC453.608875E-933.7532464
GATGGGA1300.033.7532464
GTTGATC453.608875E-933.75324616
ATGGGGG700.033.7532465
ATGGGGC200.002548884633.7532465
ATGGGCT200.002548884633.7532465
Produced by FastQC (version 0.11.5)