FastQCFastQC Report
Fri 17 Jun 2016
SRR1526015_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526015_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1242
Sequences flagged as poor quality0
Sequence length40
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT322.576489533011272No Hit
CATGATGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT181.4492753623188406No Hit
CATGATGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG90.7246376811594203No Hit
CATGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.644122383252818No Hit
CATGATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.644122383252818No Hit
CATGATGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG70.5636070853462157No Hit
CATGATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.322061191626409No Hit
CATGATGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT40.322061191626409No Hit
CATGATGGGAACTGTGGTAATTCTAGAGCTAATACATGCC40.322061191626409No Hit
CATGATGGGGACTCTGTATTTATTGTAAGGAAGTCACTGA40.322061191626409No Hit
CATGATGGGACTAATCCTAGCCCTAGCCCTACACAAATAT30.24154589371980675No Hit
CATGATGGGGAACGTCTGCCCTATCAACTTTCGATGGTAG30.24154589371980675No Hit
CATGATGGGGATGCCAAATGAAGATACAACTGAAAACACA30.24154589371980675No Hit
CATGATGGGACCCACTCCCGACCCGGGGAGGTAGTGACGA30.24154589371980675No Hit
CATGATGGGATGAAAGAACAGTATAAGTTACAGAAACTTT30.24154589371980675No Hit
CATGATGGGATCTGACTCTTTTCCTCCTGCTTTAAGAAAG30.24154589371980675No Hit
CATGATGGGATCAATGGGAGCAGTGTTTGCTATCATAGCA20.1610305958132045No Hit
CATGATGGGGGAGATCGAATCTGGATTGAGAATCAGCACT20.1610305958132045No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC20.1610305958132045No Hit
CATGATGGGATTTGAAAATGTGAAAGAAAAGTGGGTGCCT20.1610305958132045No Hit
CATGATGGGAGCCTTTCTCCTTTTCCATCTACTGTGGCTT20.1610305958132045No Hit
CATGATGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1610305958132045No Hit
CATGATGGGAACTTAAAAAAAAAAAAAAAAAAAAAAAAAA20.1610305958132045No Hit
CATGATGGGGTCCGCAGGGGGCGATTTTGGGAACCCGCTG20.1610305958132045No Hit
CATGATGGGATCTTTGTATCAGTAAGTGTGGTTTGCAGAT20.1610305958132045No Hit
CATGATGGGATAGGGTAAATAGTTAATTTTCTTCTTTCCT20.1610305958132045No Hit
CATGATGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGC20.1610305958132045No Hit
CATGATGGGTACATGGTTGATCCTGCCAGTAGCATATGCT20.1610305958132045No Hit
CATGATGGGTCAAAGATCCTATTCTGAAGGAGATTGGGCG20.1610305958132045No Hit
CATGATGGGATTGGGGGTGGGGCAGGTTTGCATAGAAGGA20.1610305958132045No Hit
CATGATGGGAGGACTTCCTAGCATTAAGCTCCACGCCAGT20.1610305958132045No Hit
CATGATGGGAGAAGATTCAGTGAAGGTTGAAGAATAGAAT20.1610305958132045No Hit
CATGATGGGGGGCAGAGTCTCAGAGCCGCCAGCGGCCCCT20.1610305958132045No Hit
CATGATGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACT20.1610305958132045No Hit
CATGATGGGTCACAGATACTCTTCTGAAGGAGATTGGGCG20.1610305958132045No Hit
CATGATGGGAAGCCATGCATGTCTAAGTACGCACGGCCGG20.1610305958132045No Hit
CATGATGGGGTGATTGATGAGATTGGGACTGCTGATAGTA20.1610305958132045No Hit
CATGATGGGGACTAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1610305958132045No Hit
CATGATGGGTCACAGATCCTCTTCTGAGAGGAGTTTTTGT20.1610305958132045No Hit
CATGATGGGTCCCTGGTTGATCCTGCCAGTAGCATATGCT20.1610305958132045No Hit
CATGATGGGGGATTTTTTGGGACTAGAATCACAGACAGTT20.1610305958132045No Hit
CATGATGGGGTTAATAAAAATATGAGCAGTCAAAAAAAAA20.1610305958132045No Hit
CATGATGGGATGAGTGTGAAGTAGCTATATATATTCTCCA20.1610305958132045No Hit
CATGATGGGGGGTGAGTTCTCTGAGGCCCGTGAGGACATG20.1610305958132045No Hit
CATGATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1610305958132045No Hit
CATGATGGGAAAAAGAAAAGGGAAAAAAATAAAACTCTAC20.1610305958132045No Hit
CATGATGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAG20.1610305958132045No Hit
CATGATGGGGCTCATGATGGGGGGAGGAAGTAAGAAGGGC20.1610305958132045No Hit
CATGATGGGAGCCTTTGAGTAGGGGAGTTTCGGTGTACAC20.1610305958132045No Hit
CATGATGGGACCAGCTGAGTGTTTAATCTTCCCATCACAG20.1610305958132045No Hit
CATGATGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT20.1610305958132045No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGAGTAAGAGC20.1610305958132045No Hit
CATGATGGGATGGTTCCTTTGGTCGCTCGCTCCTCTCCTA20.1610305958132045No Hit
CATGATGGGGAGGATCATTTATTTCACTAACAGCTGTTCT20.1610305958132045No Hit
CATGATGGGAGTTCGTGGCCATTTTCTATTCAGGACGTGG20.1610305958132045No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGGGT251.0617067E-434.04
GATGGGG600.034.04
ATGGGGT305.9052636E-634.05
ATGGGTC200.001823720633.9999965
ATGGGGG200.001823720633.9999965
CATGATG1250.032.641
ATGATGG1250.032.642
TGATGGG1200.032.5833323
GATGGGA303.1106424E-428.3333324
ATGGGAA250.005458369427.1999995
Produced by FastQC (version 0.11.5)