FastQCFastQC Report
Fri 17 Jun 2016
SRR1526014_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526014_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1107
Sequences flagged as poor quality0
Sequence length56
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT706.323396567299007No Hit
GGCTGCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC595.329719963866306No Hit
GGCTGCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC181.6260162601626018No Hit
GGCTGCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT110.9936766034327009No Hit
GGCTGCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC90.8130081300813009No Hit
GGCTGCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA80.7226738934056007No Hit
GGCTGCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA80.7226738934056007No Hit
GGCTGCGGGGCTGGCTGCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATT60.5420054200542005No Hit
GGCTGCGGGTCACAGATCCTCTTCTGAGATGAGGATCGGACGAGCTCGTATGCCGT50.45167118337850043Illumina Single End Adapter 1 (95% over 23bp)
GGCTGCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCTAATCCGCCCAATCT50.45167118337850043No Hit
GGCTGCGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC50.45167118337850043No Hit
GGCTGCGGGTCACAGATCCTCTTCTGAGATGAGGATCGGACGATATCGTATGCCGT40.36133694670280037No Hit
GGCTGCGGGGCTGGCTGCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAA40.36133694670280037No Hit
GGCTGCGGGTCACACATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT40.36133694670280037No Hit
GGCTGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT40.36133694670280037No Hit
GGCTGCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAAAAA40.36133694670280037No Hit
GGCTGCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC40.36133694670280037No Hit
GGCTGCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT30.27100271002710025No Hit
GGCTGCGGGTCACAGATCCTCTTCTGAGATGAGGATCGGAAGAGCTCGTATGCCGT30.27100271002710025Illumina Single End Adapter 2 (95% over 24bp)
GGCTGCGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCA30.27100271002710025No Hit
GGCTGCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAGTCCGCCCAATCT30.27100271002710025No Hit
GGCTGCGGGTCATAGATCCTCTTCTGGGATGAGTTTTTGTTCAATCCGCCCAATCT30.27100271002710025No Hit
GGCTGCGGGTCCCACCTCCTCTTCTGAGATGAGTTTTTGTTCCCTCCGCCCAATCT20.18066847335140018No Hit
GGCTGCGGGGCTGGCTGCGGGGAGATGAGTTTTAGTTCAATCCGCCCAAAAAAAAA20.18066847335140018No Hit
GGCTGCGGGTCCCCCATCCTCTTCTGAGATGAGTTTTTGTTCACTCCGCCCAATCT20.18066847335140018No Hit
GGCTGCGGGCACCTGAGTGCGTTTGGGATTGTTTTCTGATGTGACATATTGTCACC20.18066847335140018No Hit
GGCTGCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCGATCG20.18066847335140018No Hit
GGCTGCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCGATCT20.18066847335140018No Hit
GGCTGCGGGTCCCCCCTCCTCTTCTGAAGGAGATTGGGCGGATCGACACAAAACTC20.18066847335140018No Hit
GGCTGCGGGCAGACTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAAAAAAAAAA20.18066847335140018No Hit
GGCTGCGGGTCACAGATCCTCTTCTGAGATGAGGATCGGCAGATATCGTATGCCGT20.18066847335140018No Hit
GGCTGCGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGCTTGAACAAAAACTCA20.18066847335140018No Hit
GGCTGCGGGCACATCCTCTTCTGAGATGAGTTTTTGTTCTATCCGCCCAATCTCCT20.18066847335140018No Hit
GGCTGCGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACT20.18066847335140018No Hit
GGCTGCGGGTCACAGATCCTCTTGTGAGATGAGTTTTTGTTCAATCCGCCCAATCT20.18066847335140018No Hit
GGCTGCGAGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT20.18066847335140018No Hit
GGCTGCGGGGCTGGCTGCGGGGCTGGCTGCGGGTCACAGATCCTCTTCTGAAGGAG20.18066847335140018No Hit
GGCTGCGGGGGCTGCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCC20.18066847335140018No Hit
GGCTGCGGGTCCCCGCTCCTCTTCTGAAGGAGATTGGGCGGACTGCCCAAAAACTC20.18066847335140018No Hit
GGCTGCGGGTCACAGATCCTCTTCTGAGATGAGGATCGGCAGAGCTCGTATGCCGT20.18066847335140018Illumina Single End Adapter 1 (95% over 23bp)
GGCTGCAGGGTTCTCCGGATCTCTTCTGTGTTCCCACTACTCAAGCACCGAGTGGC20.18066847335140018No Hit
GGCTGCGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATC20.18066847335140018No Hit
GGCTGCGGGGTCTCTACTAAAAATACAAATATTAGCCAGGTGTGATGGCAGGCACC20.18066847335140018No Hit
GGCTGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.18066847335140018No Hit
GGCTGCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCAATCTC20.18066847335140018No Hit
GGCTGCGGGTCACAGATCTTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT20.18066847335140018No Hit
GGCTGCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAAACT20.18066847335140018No Hit
GGCTGCGGGTCCCCCCTCCTCTTCTGAGATGAGTTTTTGTTCCCTCCGCCCAATCT20.18066847335140018No Hit
GGCTGCGGGCTGGCTGCGGGAAGCAGTGGTATCCACGCAGCGTCGACTTTTTTTTT20.18066847335140018No Hit
GGCTGCGGGTCCCAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT20.18066847335140018No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCC251.0129526E-550.00000441
TTGTTCA202.605447E-450.037
TTCAATC202.605447E-450.040
TGTTCAA202.605447E-450.038
GGATTGA150.006375103350.040
CAATCCG351.3014869E-850.042
CGGATTG202.605447E-450.039
GTTCAAT202.605447E-450.039
GCGGATT202.605447E-450.038
CGGGTCA700.050.06
AAAACTC150.006375103350.050
GGGCGGA202.605447E-450.036
GGCGGAT202.605447E-450.037
TGCGGGT800.046.8754
GCGGGTC800.046.8755
GGGTCAC750.046.6666647
GTCACAG700.046.428579
GGTCACA700.046.428578
TCACAGA700.046.4285710
ACAGATC650.046.15384712
Produced by FastQC (version 0.11.5)