FastQCFastQC Report
Fri 17 Jun 2016
SRR1526013_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526013_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1333
Sequences flagged as poor quality0
Sequence length40
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGGGGAAGATACATGTTCCTATCAAGTCTCACCTT40.30007501875468867No Hit
GGCTGCGAGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.30007501875468867No Hit
GGCTGCGGGCTGTTCCTCGTTTTTTTTTTTTTTTTTTTTT30.22505626406601648No Hit
GGCTGCGGGCATCCTGCCTCCCTCCTTTGGGATAGGTGTG30.22505626406601648No Hit
GGCTGCGGGGCCGAATAGCCCCTCCACCCCAGCCGCCCGA30.22505626406601648No Hit
GGCTGCGGGGTACACGAGCGTCGACTTTTTTTTTTTTTTT30.22505626406601648No Hit
GGCTGCGGGGATGAGATGGACTCAAAGGAATTGACGGGTA30.22505626406601648No Hit
GGCTGCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT30.22505626406601648No Hit
GGCTGCGGGGTGGTGCAAAGACTGGAGCCACGTTTTCCTC30.22505626406601648No Hit
GGCTGCGGGGATGAGATGGACTTAAATGAATTGACGGGTA30.22505626406601648No Hit
GGCTGCGGGGACGAGAGTCTTTTTTTTTTTTTTTTTTTTT20.15003750937734434No Hit
GGCTGCGGGGAAGATACATGTTCCTATCCAGTCTCACCTT20.15003750937734434No Hit
GGCTGCGGGTAGTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.15003750937734434No Hit
GGCTGCGGGGAAGATACATGTTCCTATCACGTCTCACCTT20.15003750937734434No Hit
GGCTGCGGGGCCGAATAGCCTCTCCACCCCCGCCGCCCGA20.15003750937734434No Hit
GGCTGCGGGATCGAGAGTCACTTTTTTTCTTTTTTTTTTT20.15003750937734434No Hit
GGCTGCGGGACTTGTAATTCTTTTCTTGGTCCTGTGTGAG20.15003750937734434No Hit
GGCTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC20.15003750937734434No Hit
GGCTGCGGGGGGGTTGGGGATTTAGCTCCGTGGTCGCGCG20.15003750937734434No Hit
GGCTGCGGGACAAGAGTCGCTTTTTTCTTTTTTTTTTTTT20.15003750937734434No Hit
GGCTGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT20.15003750937734434No Hit
GGCTGCGGGAAGTACTGCCTTTGAGAGCACCCACCCATTC20.15003750937734434No Hit
GGCTGCGGGGCTGGCTGCGGGCTCATTTGCTGTGCTGGTT20.15003750937734434No Hit
GGCTGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA20.15003750937734434No Hit
GGCTGCGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.15003750937734434No Hit
GGCTGCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT20.15003750937734434No Hit
GGCTGCGGGGTGGTGCCAAGACTGGAGCCCCCTTTTCCTC20.15003750937734434No Hit
GGCTGCGGGTGGCTCGACTTTTTTTTTTTTTTTTTTTTTT20.15003750937734434No Hit
GGCTGCGGGTGGTATCCGCAGATCGACTTTTTTTTTTTTT20.15003750937734434No Hit
GGCTGCAGGAAGACACGCCGAGCCCTTTTTTTTTTTTTTT20.15003750937734434No Hit
GGCTGCGGGGCTGACCCTGAGGTGCAGCAGATCATGAGTG20.15003750937734434No Hit
GGCTGCGGGGTGTTCACGCATAGTCGATTTTTTTTTTTTT20.15003750937734434No Hit
GGCTGCGGGTGGACGAGTTTTTTTTTTTTTTTTTTTTTTT20.15003750937734434No Hit
GGCTGCGGGAGTCGCCTTTTTTTTTTTTTTTTTTTTTTTT20.15003750937734434No Hit
GGCTGCGGGGATGAGATGGACTCAAATGAATTGACGGGTA20.15003750937734434No Hit
GGCTGCGGGGATGAGATGGACTTAAAGGAATTGACGGGTA20.15003750937734434No Hit
GGCTGCGGGGGGTCTTGTGTGTGAGTCTGTATCAGCTGAC20.15003750937734434No Hit
GGCTGCGGGGCCGAATAGCCTCTCCACCCCAGCCGCCCGA20.15003750937734434No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGGGC251.1207712E-434.04
GCGGGGC200.001888579233.9999965
CGGGGCT200.001888579233.9999966
GCTGCGG1350.031.4814822
TGCGGGG551.1823431E-1030.909094
GGCTGCG1400.030.357141
CTGCGGG1400.030.357143
TGCGGGA455.1453753E-830.2222214
Produced by FastQC (version 0.11.5)