FastQCFastQC Report
Fri 17 Jun 2016
SRR1526012_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526012_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98
Sequences flagged as poor quality0
Sequence length40
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGGGGGAGTGATCTTGTTCCCGACACTTCCTATTT11.0204081632653061No Hit
GGCTGCGGGCCGAAAAAACAAAAAAACAAAAAACAAAAAA11.0204081632653061No Hit
GGCTGCGGGCGGGGTTTTTTGTTTGTTTGGTTGGTTGGGG11.0204081632653061No Hit
GGCTGCGGGTGAAAGCTGCAACAATACTTTTCCCGGCACT11.0204081632653061No Hit
GGCTGCGGGGTGCTGGCTGCCTGTGTTCCCCCCAGGAGGC11.0204081632653061No Hit
GGCTGCGGGCGTGGTCCCCCCCGACCCCCCCTTGCGTTGC11.0204081632653061No Hit
GGCTGCGGGGGACGAAGATCACTTCACCCCCACGGCCTCC11.0204081632653061No Hit
GGCTGCGGGGCGAGTTTGTGGAAGATGGCGCCCGTTGTGA11.0204081632653061No Hit
GGCTGCGGGGTTTTCCAGGCCTGTTCAAATCCTGTTGTCT11.0204081632653061No Hit
GGCTGCGGGTTCTGGTATGTCTTCAAAATGAGTCCACGAG11.0204081632653061No Hit
GGCTGCGGGCCAGCATCTTATCCACCCTTCCGCTCCGCTC11.0204081632653061No Hit
GGCTGCGGGTTGTGCTTTTTTTTTTTTTTTTTTTTTTTTT11.0204081632653061No Hit
GGCTGCGGGATTCCCTGAAGAGAATGCCACCCCGAAGATA11.0204081632653061No Hit
GGCTGCGGGCTGGCTCCCCCCGCGTCCCCTTTTTTTTTTT11.0204081632653061No Hit
GGCTGCGGGTGGACAACGCCGACCACCTTTTTTCTTTTTT11.0204081632653061No Hit
GGCTGCGGGTGGTATCCACCAGCGTTTTTTTTTTTTTTTT11.0204081632653061No Hit
GGCTGCGGGAAGCATGGTCTCACCGCCAAGCACACACCAC11.0204081632653061No Hit
GGCTGCGGGAAGAGTGGCCTCAACCAGAATCCCCTTTTTT11.0204081632653061No Hit
GGCTGCGGGGCTGGTGCCGGGCCCAAACACACCACCACCC11.0204081632653061No Hit
GGCTGCGGGTCCGATCTTTTTTTTTTTTTTTTTTTTTTTT11.0204081632653061No Hit
GGCTGCGGGGCTGGCTGCGGGAAGCAGTGGTATCAAACAC11.0204081632653061No Hit
GGCTGCGGGCTGTCCCCGCCCCGTCGCCTTTTTTTTTTTT11.0204081632653061No Hit
GGCTGCGGGATGAGATGGACTCCACCCCCCCCTATGCCTT11.0204081632653061No Hit
GGCTGCGGCCCAGCTCCCCCTCTTTTTTTTTTTTTTTTTT11.0204081632653061No Hit
GGCTGCGGGTTCACGCCGCGCCCATCGGAAGATCTCGTAT11.0204081632653061No Hit
GGCTGCGGGGCTGCCTCCGGGGGGGGCAAACACACAAACC11.0204081632653061No Hit
GGCTGCGGGCGAGTGTCCCAGAGTCTTTTTTTTTTTTTTT11.0204081632653061No Hit
GGCTGCGGGGCTAGGCGTGGCACCCTGGCTCCCCAGGAGC11.0204081632653061No Hit
GGCTGCGGGTCCCACCCCCCCGAAACTCATCCCGGGCTCC11.0204081632653061No Hit
GGCTGCGGGGCATGTTTGGCCTTATTTTTTATACACACCT11.0204081632653061No Hit
GGCTGCGGGCTCAACGTAGAGTCGCCTTTTTTTTTTTTTT11.0204081632653061No Hit
GGCTGCGGGGGCATCAGACTTTTTGAACTAATACGCTTTA11.0204081632653061No Hit
GGCTGCGGGATGAGATGGCCCCAAATGGATTGCCGGGTCC11.0204081632653061No Hit
GGCTGCGGGGTCAAAACTGCTGGTATCACCCCCTCTTTGC11.0204081632653061No Hit
GGCTGCGGGACCGTGTATAACCCCCCGTCCCCTTTTTTTT11.0204081632653061No Hit
GGCTGCGGGGTGCTGGCTGCCGGAGTATCCCCGCAGAGTC11.0204081632653061No Hit
GGCTGCGGTTAGAGTCGTTTTTTTTTTTTTTTTTTTTTTT11.0204081632653061No Hit
GGCTGCGGGCCCCCTTAACCGAGCCCCCTTTTTTTTTTTT11.0204081632653061No Hit
GGCTGCGGGCACCCGTGGTCTCAACCCAAAAAAAAAAAAA11.0204081632653061No Hit
GGCTGCGGGTACCCCAGAGCGCCCTTTTTTTTTTTTTTTT11.0204081632653061No Hit
GGCTGCGGGGCCCCCTTGCCCGCGGTTTCTTTTCCCTTTC11.0204081632653061No Hit
GGCTGCGGGAAGCATACACCCAGATCGCCTTTTTTTTTTT11.0204081632653061No Hit
GGCTGCGGGTCCAGAGTCCTTTTGTTTTTTTTTTTTTTTT11.0204081632653061No Hit
GGCTGCGGGGACAACCCAAAAAAAAAAAAAAAAAAAAAAA11.0204081632653061No Hit
GGCTGCGGGGAAAACAAACAAAAAAAAAAAAAAAAAAACA11.0204081632653061No Hit
GGCTGCGGGGTCGTCCTTGTTTTTTCTTTTTTTTTTTCTT11.0204081632653061No Hit
GGCTGCGGGGGATTCCACCCTCCCTCACAACAGATTGCCT11.0204081632653061No Hit
GGCTGCGGGAGAGTGGTCCCCCCCGCGCCCCCTTTTTTTT11.0204081632653061No Hit
GGCTGCGGGAAGCAGAGGTATCAACGTTAACAAAACCGTA11.0204081632653061No Hit
GGCTGCGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTCC11.0204081632653061No Hit
GGCTGCGGGGTTTTCAAGGCCTGTTAAAATTCTGTTGTAT11.0204081632653061No Hit
GGCTGCGGGCCTTTCCAGTATGTCCCAACAGAAGGGAGTT11.0204081632653061No Hit
GGCTGCGGGCAGCAGTGGTTCCACGTACAAAACCCCACAC11.0204081632653061No Hit
GGCTGCGGGGTGCTGGCCGCCGGAGTATCCACCCCGAGTC11.0204081632653061No Hit
GGCTGCGGCTGGCTGCGGGCCGAGTGGTCTCCCCACACCC11.0204081632653061No Hit
GGCTGCGGGCCGCAGTGGTATCCACCCAAAAAAACACACC11.0204081632653061No Hit
GGCTGCGGGCCCTCCATTTTTTTTTTTTTTTTTTTTTTTT11.0204081632653061No Hit
GGCTGCGGGACGATGGACACCCCGAGCCCACTTTTTTTTT11.0204081632653061No Hit
GGCTGCCGGCCTGGTTCACCCAAAAAAAAAACCACACACC11.0204081632653061No Hit
GGCTGCGGGTGCTGCTGAGCCCTTTCTGGGATGTTGTCCT11.0204081632653061No Hit
GGCTGCGGGATCATCCGAACCCCCCCCCCTCCGCCCCCCG11.0204081632653061No Hit
GGCTGCGGGATTGATATTCGTAGTACATCATAAACCTTGC11.0204081632653061No Hit
GGCTGCGGGGGAATCAGACTTTTTGAACTAATAAGCTTTA11.0204081632653061No Hit
GGCTGCGGGGCTGCCTGCGGGAAGCATGGCATCACCACAA11.0204081632653061No Hit
GGCTGCGGGAAGATGGCCACCCCGAGTCGCCTTTTTTTTT11.0204081632653061No Hit
GGCTGCGGGGCATTCACCCAGACCCCACCTTTTTTTTTTT11.0204081632653061No Hit
GGCTGCGGGTCCACAGACCACTTCTTTTTTTTTTTTTTTT11.0204081632653061No Hit
GGCTGCGGGCGAGCATCTTATCCACCCTTCCCTTCCCCTC11.0204081632653061No Hit
GGCTGCCGGAACAGTCCACTCCAGCCCCAATACTCTCTAC11.0204081632653061No Hit
GGCTGCGGGGAGTCCCCTTTTTTTTTTTTTTTTTTTTTTT11.0204081632653061No Hit
GGCTGCCGGGGCACACAACAAAAAAAAAAAAAAAAAAAAA11.0204081632653061No Hit
GGCTGCGGGAAGCCGTGGTATCCACCCAACCCCCCAAACC11.0204081632653061No Hit
GGCTGCGGGCCGCCTCTCCCCCCCCCCCCCCCTTTTCTTT11.0204081632653061No Hit
GGCTGCGGGGCTGGCTGCCGGGCCCCCCCCTCCCCCCTTC11.0204081632653061No Hit
GGCTGCGGGGCCGGAGCTGGTGCTGATGAGGCCGAGGCTT11.0204081632653061No Hit
GGCTGCGGGTTTTAGTTTACAGTCCTGTGTGCTTCCCTGT11.0204081632653061No Hit
GGCTGCGGGGAGCAGTGTCGCCTCGCCTTTTTTTTTTTTT11.0204081632653061No Hit
GGCTGCGGGCAGTGCTGAGATCGGGGTAACAAAACACACC11.0204081632653061No Hit
GGCTGCGGGACGCCGGTATCAACCCCGAGTCCCCTTCTTT11.0204081632653061No Hit
GGCTGCGGGGCTGGCTCCGGGAGTGGNCAACGCAGACGCG11.0204081632653061No Hit
GGCTGCGGGAGCCATTTCTCTTTTTTTTTTTTTTTTTTTT11.0204081632653061No Hit
GGCTGCGGGCTGTCGCCCCCTTTTTTTTTTTTTTTTTTTT11.0204081632653061No Hit
GGCTGCGGGAGTGGTACGCAGAGCCGACTATTTTTGTTTT11.0204081632653061No Hit
GGCTGCGGGGGTATGAACCCATGACAAACCTGGCAACAGT11.0204081632653061No Hit
GGCTGCGGGAGCGTTATCACCCCCCCGCCTTTTTTTTTTT11.0204081632653061No Hit
GGCTGCCGGGATGAGATGCACTTAAAGGAATTGCCGGGCA11.0204081632653061No Hit
GGCTGCGGGCCTTTTTTTTTTTTTTTTTTTTTTCTCCTTT11.0204081632653061No Hit
GGCTGCGGGGAGTGTGAGTGTAAGTGTGCGTATGCCGTCT11.0204081632653061No Hit
GGCTGCGGGGAAGATCACCCCGGACAACCCTTCCATCGCC11.0204081632653061No Hit
GGCTGCGGGCGCCCCCCCCCCCCCNCCCCCCCCCCCCCCC11.0204081632653061No Hit
GGCTGCGGGTCCACCCACCCCACTTTTTTTTTTTTTTTTT11.0204081632653061No Hit
GGCTGCGGGGACTGCAGGGACAACATAGCCTCCCATGAAC11.0204081632653061No Hit
GGCTGCGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.0204081632653061No Hit
GGCTGCGGGAAAAACAAACAAAAAAAAAAAAAACACAAAA11.0204081632653061No Hit
GGCTGCGGGGGGGGGCAAAGGGCCCGTTTGCCTGACTCCA11.0204081632653061No Hit
GGCTGCGGGGAATAATTAACAAAACCCCCTTTACCCCTTC11.0204081632653061No Hit
GGCTGCGGGCACTGAGACTGGCCTCTTTTTTTCTCTCCCG11.0204081632653061No Hit
GGCTGCGGGTGGTCCCCCCCCCGCCCCCTTTTTTTTTTTT11.0204081632653061No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTCC50.034.029
GCGGGGC50.034.05
TTTCTTT50.034.026
TTTCCCT50.034.031
CGGGGCC50.034.06
GCGGTTT50.034.022
TTTTCCC50.034.030
GTTTCTT50.034.025
GGTTTCT50.034.024
CCTTGCC50.034.014
GGCTGCG50.034.01
TTCCCTT50.034.032
TGCCCGC50.034.017
CCCGCGG50.034.019
CTGCGGG50.034.03
GGCCCCC50.034.09
GCTGCGG50.034.02
TTCTTTT50.034.027
CGCGGTT50.034.021
CTTGCCC50.034.015