FastQCFastQC Report
Fri 17 Jun 2016
SRR1526011_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526011_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71
Sequences flagged as poor quality0
Sequence length40
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT57.042253521126761No Hit
GGCTGCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG34.225352112676056No Hit
GGCTGCGGGTCAACGCAGAGTCGACTTTTTTTTCTTTTTT22.8169014084507045No Hit
GGCTGCGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG22.8169014084507045No Hit
GGCTGCGGGTCACAGATCCCCTTCTGAGATTAGTTTTTGT11.4084507042253522No Hit
GGCTGCGGGTCACAGATACTCTCCTGAAGGAGATTGGGCG11.4084507042253522No Hit
GGCTGCGGGCACTGCTCATTTTTAGCACTAATGTCTGATC11.4084507042253522No Hit
GGCTGCGGGGCTGGCTGCGGGTCACAGATCCTCTTCTGAG11.4084507042253522No Hit
GGCTGCGGGCAGTGCTGGCTGGATCGGAAGAGCTCGTATG11.4084507042253522No Hit
GGCTGCGGGTCACCGATCCCCTGCAGGAGGATCGGAAGAG11.4084507042253522No Hit
GGCTGCGGGCACAGATGCTTACACCACATGAAACACTGTC11.4084507042253522No Hit
GGCTGCGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT11.4084507042253522No Hit
GGCTGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC11.4084507042253522No Hit
GGCTGCGGGTCAAAGATACTCTTCTTAGATTAGTTTTTGT11.4084507042253522No Hit
GGCTGCGGGTCAAAGATCCCCTTCTGAGATGAGTTTTTGT11.4084507042253522No Hit
GGCTGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.4084507042253522No Hit
GGCTGCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTAT11.4084507042253522No Hit
GGCTGCGGGTCACAAATCCTCTTCTGAGATGAGTTGTTGG11.4084507042253522No Hit
GGCTGCGGGTCACAGATCCTCTTCTGAAGGAGATTGGTCG11.4084507042253522No Hit
GGCTGCGGGTCACAGATCCCCTTCTGAGATGAGTTTTTGT11.4084507042253522No Hit
GGCTGCGGGTTACAGATCCTCTTCTGAGATGAGTTTTTGT11.4084507042253522No Hit
GGCTGCGGGGATAGCAATAATAGCTCTACTAAACCTATTC11.4084507042253522No Hit
GGCTGCGGGTCACAAATCCTCTTCTGAGATGAGTTTTTGT11.4084507042253522No Hit
GGCTGCGGGAAGCAATGGTATCAACGCGCAGTCGACTTTT11.4084507042253522No Hit
GGCTGCGGTCACAGATCCTCTTCTGAAGTAGATTGGGCGG11.4084507042253522No Hit
GGCTGCGGGTCAACGATCCTCTTCTGAGATGAGTTTTTGT11.4084507042253522No Hit
GGCTGCGGGCCTCTTCTGAAGGAGATTGGGCGGATTGAAC11.4084507042253522No Hit
GGCTGCGGGACAGTTAAAGCAGAGTCGACTTTTTTTTTTT11.4084507042253522No Hit
GGCTGCGGGTCACAGATCCTCTTCTGAGATGATTTTTTGT11.4084507042253522No Hit
GGCTGCGGGGCTGGCTGCGGGTACAAAAAAAAAAAAAAAA11.4084507042253522No Hit
GGCTGCGGGTCACAGAGAACACTACAAGAAGGCATCACAC11.4084507042253522No Hit
GGCTGCGGGGGCTGCGGGCCACAGATTCTCTTCTGAGATG11.4084507042253522No Hit
GGCTGCGGGGCTGGCTGCGGGAAGCATGGTATCAACCCAA11.4084507042253522No Hit
GGCTGCGGGTCAACGCCGACGACTTTTTTTTTTTTTTTTT11.4084507042253522No Hit
GGCTGCGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCG11.4084507042253522No Hit
GGCTGCGGGTCACAAATCTTCTTCTGAAGGAGATTGGGCG11.4084507042253522No Hit
GGCTGCGGGCTGGCTGAGGGTTGGACAAAAACTCATCTCA11.4084507042253522No Hit
GGCTGCGGGTCACAGATCCTCTGAAGGAGATTGGGCGGAT11.4084507042253522No Hit
GGCTGCGGGTCAAAAATCCCCCTCTGAGAAGAGTTTTTGT11.4084507042253522No Hit
GGCTGCGGGTCCAAGATCTTCTGAAGTGGAGACCAACGCG11.4084507042253522No Hit
GGCTGCGGGTCAACTATCCTCTTCTTAGATGAGGATCGGA11.4084507042253522No Hit
GGCTGCGGGACGCAGTGGTATCAAAAAAAAAAAAAAAAAA11.4084507042253522No Hit
GGCTGCGGGTGAGTTTTTGTTCAATAAGCCCAGTATCATT11.4084507042253522No Hit
GGCTGCGGGGGAACCAAAAAAAAAAAAAAAAAAAAAAAAA11.4084507042253522No Hit
GGCTGCGGGTCCCAGATCCTTTCCTGAAGGAGATTGGGAG11.4084507042253522No Hit
GGCTGCGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.4084507042253522No Hit
GGCTGCGGGGAGTGCAGGCTCAACATAAAATTTCAATTAA11.4084507042253522No Hit
GGCTGCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCT11.4084507042253522No Hit
GGCTGCGGGAAGATGGTTCCTGCTTATGTGGGAGGCATGT11.4084507042253522No Hit
GGCTGCGGGTCAAAGCTACTCTTCTGAAGGAAATTGGTCG11.4084507042253522No Hit
GGCTGCGGGTTCAGTTTACAATTGGAAGACTCATAGTTCC11.4084507042253522No Hit
GGCTGCGGGCTGTCTTTACGTGGTGGCCTTAAGCACCGTC11.4084507042253522No Hit
GGCTGCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCG11.4084507042253522No Hit
GGCTGCGGGACAGATCCTCTTCTGAAGGAGAATGGGCGGA11.4084507042253522No Hit
GGCTGCGGGTCCAAGATCATCTAAGTAGATTGGGCGGATT11.4084507042253522No Hit
GGCTGCGGGCCTCTTCTGAAGGAGATTGAGCGGATTGAAC11.4084507042253522No Hit
GGCTGCGGGTCAAAGATCCTCTTCTGAAAGAGATTGGGAG11.4084507042253522No Hit
GGCTGCGGGTCAAACATCCTCTTCTGAAGGAGATTGGGCG11.4084507042253522No Hit
GGCTGCGGGAAGAAGTGGTATTACCGCATAGATCGGAAGA11.4084507042253522No Hit
GGCTGCGGGTAAAGTGAAACTGCGAATGGCTCATTAAATC11.4084507042253522No Hit
GGCTGCGGTCAAATCCTCTTCTTAGATGAGTTTTTGTTCA11.4084507042253522No Hit
GGCTGCGGGTCACCGCTCCTCTTCTGAAGGAGATTGGGCG11.4084507042253522No Hit
GGCTGCGGGGGCTGCGGGTCACAGATCCTCTTCTGAGATG11.4084507042253522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCAG50.034.027
TTGTTCA50.034.017
GGTGAGT50.034.08
CCCAGTA50.034.029
GCCCAGT50.034.028
TTCAATA50.034.020
ATAAGCC50.034.024
CCAGTAT50.034.030
CAGTATC50.034.031
TAAGCCC50.034.025
TTTTTGT50.034.014
TGTTCAA50.034.018
GTTTTTG50.034.013
GTATCAT50.034.033
GGCTGCG50.034.01
GTTCAAT50.034.019
TCAATAA50.034.021
GAGTTTT50.034.011
GTGAGTT50.034.09
AGTATCA50.034.032
Produced by FastQC (version 0.11.5)