FastQCFastQC Report
Fri 17 Jun 2016
SRR1526010_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526010_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29660
Sequences flagged as poor quality0
Sequence length56
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCATGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT2260.7619689817936615No Hit
TAGCATGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC1930.6507080242751181No Hit
TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG1670.5630478759271746No Hit
TAGCATGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA730.2461227242076871No Hit
TAGCATGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT600.20229265003371544No Hit
TAGCATGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC580.19554956169925827No Hit
TAGCATGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT560.18880647336480108No Hit
TAGCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC550.18543492919757248No Hit
TAGCATGGGACTAATCCTAGCCCTAGCCCTACACAAATATCATTATACTATTATAT470.15846257585974377No Hit
TAGCATGGGGAGCTCCATTATGCTTTGGCAGGTGATCAGACTGAAGCGTAAATGGA400.13486176668914363No Hit
TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG360.12137559002022927No Hit
TAGCATGGGGATCCCCTCGACCACTCTTTTGGCGCTTCGCTGTCGACAGTGCGCTT340.11463250168577209No Hit
TAGCATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA320.10788941335131491No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAATT150.00786633350.75903738
ATAATTA150.00786633350.75903739
TAATTAT150.00786633350.75903740
GCGCATT309.127525E-750.75903738
CGCATTG309.127525E-750.75903739
ACTGACT150.00792012850.6718241
AATTATA150.00792012850.6718241
CATTGAA203.8541263E-450.67181841
CGTCTCA203.8541263E-450.67181841
GGCGCAT309.3475865E-750.58490837
TGCTCGT150.00797419650.58490837
AATACCA150.00797419650.58490837
TATGCTC150.00802853950.4982935
AGAGCGC150.00802853950.4982935
AATATAA150.00802853950.4982936
ATCCTAG252.0008696E-550.15476214
ACTAATC252.0008696E-550.15476210
TGGTTGA402.4629117E-950.15476214
AATCCTA252.0008696E-550.15476213
GGTTGAT402.4629117E-950.15476215