##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526010_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 29660 Sequences flagged as poor quality 0 Sequence length 56 %GC 51 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.55128118678355 38.0 36.0 39.0 31.0 40.0 2 33.70502360080917 38.0 33.0 39.0 15.0 40.0 3 35.57670262980445 38.0 35.0 39.0 28.0 40.0 4 35.59898853674983 38.0 35.0 39.0 28.0 40.0 5 33.212845583277144 38.0 32.0 39.0 15.0 40.0 6 35.81166554281861 38.0 35.0 39.0 28.0 40.0 7 36.34190829399865 38.0 36.0 39.0 30.0 40.0 8 36.03310856372219 38.0 36.0 39.0 30.0 40.0 9 35.96159811193527 38.0 36.0 39.0 29.0 40.0 10 35.13590694538099 38.0 35.0 39.0 26.0 40.0 11 35.16797033041133 38.0 35.0 39.0 28.0 40.0 12 34.10795684423466 38.0 33.0 39.0 23.0 40.0 13 33.42784895482131 38.0 32.0 39.0 19.0 40.0 14 34.14740391099124 38.0 33.0 39.0 23.0 40.0 15 33.077781523937965 38.0 32.0 39.0 18.0 40.0 16 32.689480782198245 37.0 30.0 39.0 19.0 40.0 17 31.664531355360754 36.0 28.0 38.0 15.0 40.0 18 31.06035064059339 35.0 27.0 38.0 14.0 40.0 19 32.18361429534727 36.0 29.0 38.0 18.0 40.0 20 33.00836142953473 37.0 30.0 38.0 22.0 40.0 21 33.34463924477411 38.0 31.0 39.0 20.0 40.0 22 32.741672285906944 38.0 30.0 39.0 17.0 40.0 23 33.27973701955496 38.0 31.0 39.0 20.0 40.0 24 32.608934592043155 37.0 30.0 39.0 18.0 40.0 25 33.10148347943358 38.0 31.0 39.0 20.0 40.0 26 33.52511800404585 38.0 33.0 39.0 20.0 40.0 27 33.79322319622387 38.0 33.0 39.0 23.0 40.0 28 33.810856372218474 38.0 33.0 39.0 23.0 40.0 29 33.32825354012137 38.0 32.0 39.0 20.0 40.0 30 33.147943358057994 38.0 32.0 39.0 19.0 40.0 31 33.81635198921106 38.0 33.0 39.0 23.0 40.0 32 33.68550236008092 38.0 33.0 39.0 22.0 40.0 33 33.49157113958193 38.0 33.0 39.0 22.0 40.0 34 33.40782198246797 38.0 33.0 39.0 20.0 40.0 35 33.38890761968982 38.0 33.0 39.0 20.0 40.0 36 32.67231962238705 37.0 32.0 39.0 17.0 40.0 37 32.44433580579906 37.0 32.0 38.0 16.0 40.0 38 31.851550910316924 36.0 30.0 38.0 10.0 40.0 39 30.59855023600809 35.0 28.0 38.0 4.0 40.0 40 28.660991233985165 35.0 23.0 38.0 4.0 39.0 41 26.687356709372892 32.0 17.0 38.0 4.0 39.0 42 27.57171274443695 33.0 21.0 38.0 4.0 39.0 43 27.66001348617667 33.0 22.0 38.0 4.0 39.0 44 26.6944032366824 32.0 18.0 38.0 4.0 39.0 45 27.63509777478085 33.0 22.0 38.0 4.0 39.0 46 25.624578556979095 30.0 16.0 37.0 4.0 39.0 47 24.601517194875253 29.0 10.0 36.0 4.0 39.0 48 25.234592043155764 30.0 15.0 36.0 4.0 39.0 49 24.23762643290627 29.0 9.0 36.0 4.0 39.0 50 24.968948078219825 30.0 12.0 36.0 4.0 39.0 51 25.21736345246123 31.0 7.0 37.0 4.0 39.0 52 25.05724881995954 31.0 4.0 36.0 4.0 39.0 53 24.8354012137559 31.0 4.0 36.0 4.0 39.0 54 24.46888064733648 30.0 4.0 36.0 4.0 39.0 55 24.153776129467296 30.0 4.0 36.0 4.0 39.0 56 22.853776129467295 29.0 4.0 36.0 4.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 3.1738380859135518 1 2 6.518782503228554 1 3 4.459077078267093 1 4 4.001736764812044 1 5 7.168942550626422 1 6 3.9974701446298724 1 7 3.562715009483462 1 8 3.6490078796803758 1 9 3.8339756366849684 1 10 4.683339451346086 1 11 -0.2376523764276186 1 12 -6.945037800565988 1 13 -19.014956342957177 1 14 -6.824025973611953 1 15 0.41051118469414405 1 16 -2.710046692040301 1 17 -5.953414189558874 1 18 -4.983634295146032 1 19 -0.6767421055794323 1 20 -2.154915968056091 1 21 5.628122479852681 1 22 6.186491038177913 1 23 6.25250814808334 1 24 6.022539452106887 1 25 5.749880595147385 1 26 5.007417154892686 1 27 4.679314949068953 1 28 4.715261776259958 1 29 5.787160331383365 1 30 4.904488736755692 1 31 5.131732435427111 1 32 4.579138350644627 1 33 4.547884142025371 1 34 4.178067687840237 1 35 5.486404052076875 1 36 4.030332487598937 1 37 4.67514986270481 1 38 4.290121701834472 1 39 4.568245286299842 1 40 -10.373984475262688 1 41 -2.213760008718463 1 42 -3.5586883054538845 1 43 -10.091737971394002 1 44 -15.418549030079937 1 45 -4.645977539849703 1 46 -5.701820701696498 1 47 -10.404923958547382 1 48 -6.1944188330981795 1 49 -12.79600197826778 1 50 -3.2670361564760704 1 51 0.6245867853122 1 52 0.5151112266562059 1 53 0.7933097785410048 1 54 0.7089381629579314 1 55 0.8045427217954426 1 56 4.4178092079047815 2 1 3.351977620797271 2 2 6.8829685497401805 2 3 4.633495682918259 2 4 4.345457695044601 2 5 7.979175108765961 2 6 4.218400377188011 2 7 3.9808545443671832 2 8 3.546217182005961 2 9 4.154905869243109 2 10 4.993572009485625 2 11 3.5972313445026103 2 12 0.45728778082936117 2 13 3.3343459826242174 2 14 4.718764724062467 2 15 5.250046068415077 2 16 5.3336742381922555 2 17 7.528911391836473 2 18 5.472179658342341 2 19 6.324188126978708 2 20 5.478107287757862 2 21 7.02719224729454 2 22 7.174863131201171 2 23 5.320415124827523 2 24 6.724399917223163 2 25 6.538717804449707 2 26 5.772533433962451 2 27 5.070942856045697 2 28 5.197587357655308 2 29 5.074602191848488 2 30 6.131465480941738 2 31 4.3061510400782765 2 32 3.9740220715748578 2 33 3.639046932723044 2 34 3.907835129700697 2 35 4.5501249823094305 2 36 5.109402255040795 2 37 5.325382420844345 2 38 5.9664007716019185 2 39 5.664059239788216 2 40 5.945550408458242 2 41 9.717867898258284 2 42 8.834334950360066 2 43 7.456634121629257 2 44 -3.3571536812427283 2 45 8.854487576429367 2 46 7.952132786675595 2 47 5.13368069261541 2 48 7.759999771552984 2 49 0.4007422077787304 2 50 6.909242913291369 2 51 10.225051901591272 2 52 8.834646110377136 2 53 9.34633303435496 2 54 9.49033351179514 2 55 9.135240396214048 2 56 11.422925486974549 3 1 3.5483041514095177 3 2 6.38541752933202 3 3 4.480434458428462 3 4 4.477294429738478 3 5 7.4257057210108535 3 6 4.453094254739035 3 7 3.753507605591672 3 8 4.322951875883511 3 9 3.840620154957392 3 10 4.327857723771338 3 11 3.7870272628699624 3 12 5.31647145429875 3 13 5.888223533644624 3 14 4.991825948552261 3 15 0.6520868847416033 3 16 6.500204850437154 3 17 7.552993024489538 3 18 8.455853127730094 3 19 7.085004453509317 3 20 5.856474634696635 3 21 6.601069798314949 3 22 7.654454967935862 3 23 6.982455941154054 3 24 7.219501958039491 3 25 6.384432090163994 3 26 5.69702322988082 3 27 5.354208162168142 3 28 4.9359547045940815 3 29 5.085214436746444 3 30 6.26044507277847 3 31 3.9490081829354224 3 32 4.379328194023834 3 33 4.58680203476386 3 34 3.900896354190497 3 35 4.8803290639420815 3 36 5.962463479530594 3 37 4.9727293596198585 3 38 6.875788526703957 3 39 6.742426586726985 3 40 8.581876939070487 3 41 11.277459734992973 3 42 10.57515127689068 3 43 10.559899427751702 3 44 10.877132033042987 3 45 10.13285492336814 3 46 12.377438909124571 3 47 13.44225212118684 3 48 12.337142628695837 3 49 13.20115037104404 3 50 11.80842658676076 3 51 10.48505190159127 3 52 10.634237947111828 3 53 10.008782013946796 3 54 10.229109021999221 3 55 10.636056722744659 3 56 9.597211201260265 4 1 3.4182521306011964 4 2 6.635517569348028 4 3 4.506044702526104 4 4 3.9638890675936196 4 5 7.059959422491449 4 6 4.110557239933108 4 7 3.963991799269145 4 8 4.113668162398113 4 9 4.0843176339489915 4 10 4.4371014212503255 4 11 3.6109568346986904 4 12 4.729836800437205 4 13 4.84140480615363 4 14 5.083862763278148 4 15 5.648085284101352 4 16 6.624654630349124 4 17 6.937146685954119 4 18 7.801591423048222 4 19 7.279482244625765 4 20 6.21261709167943 4 21 6.691505972784736 4 22 6.812118033161958 4 23 6.096101399337332 4 24 6.464399917223165 4 25 6.0595021181752 4 26 5.374494218276176 4 27 5.4262369736927525 4 28 4.82150892628276 4 29 5.789896309495543 4 30 5.144798814275074 4 31 4.296347118509651 4 32 4.721865208829762 4 33 5.355909677821085 4 34 3.639991992445797 4 35 4.781889688191786 4 36 6.17214735308001 4 37 5.1936177149619915 4 38 6.131890967680349 4 39 6.7064121809646835 4 40 2.1380994280660843 4 41 10.748848290415147 4 42 10.024531028791444 4 43 9.004477258884158 4 44 11.005983573659233 4 45 9.155664047017599 4 46 11.916054355303046 4 47 11.445053241635016 4 48 12.401568399003963 4 49 12.20035005091599 4 50 12.300223305448235 4 51 11.36740484276774 4 52 11.595822580965368 4 53 11.72947028925692 4 54 11.270725668657882 4 55 11.948181572684636 4 56 12.902533330111808 5 1 3.6069776207972737 5 2 7.012968549740183 5 3 4.7168290162515945 5 4 4.343791028377936 5 5 7.953341775432623 5 6 4.614233710521347 5 7 3.725021211033848 5 8 3.8195505153392943 5 9 3.888239202576443 5 10 3.946905342818958 5 11 4.172231344502613 5 12 5.1906211141626954 5 13 5.673512649290881 5 14 4.8595980573958 5 15 5.654212735081739 5 16 6.652840904858923 5 17 7.786411391836474 5 18 8.251346325009003 5 19 6.7525214603120425 5 20 5.4381072877578625 5 21 6.511358913961207 5 22 6.904029797867835 5 23 6.651248458160861 5 24 6.6935665838898295 5 25 6.598717804449709 5 26 5.4308667672957895 5 27 4.107609522712359 5 28 5.475920690988644 5 29 5.471268858515149 5 30 5.769798814275074 5 31 4.389484373411612 5 32 4.905688738241523 5 33 4.032380266056382 5 34 4.6645017963673645 5 35 4.7034583156427665 5 36 5.3302355883741335 5 37 5.188715754177682 5 38 5.3855674382685805 5 39 7.490725906454877 5 40 8.786383741791578 5 41 10.568701231591618 5 42 10.326001617026733 5 43 9.47996745496259 5 44 10.412846318757271 5 45 9.151987576429367 5 46 11.94546612000893 5 47 10.63868069261541 5 48 10.688333104886318 5 49 10.430742207778732 5 50 10.182576246624706 5 51 7.8600519015912695 5 52 8.612979443710469 5 53 8.427999701021626 5 54 8.248666845128472 5 55 9.261907062880717 5 56 7.903758820307882 6 1 3.3841515338407504 6 2 6.523838114957574 6 3 4.916104378570431 6 4 4.363718564609819 6 5 7.903522934852912 6 6 4.479269942405402 6 7 3.968680631323707 6 8 4.247086747223349 6 9 4.228818912721366 6 10 4.700528531224755 6 11 4.862448735806964 6 12 5.270331259090227 6 13 5.976954678276389 6 14 4.817025593627683 6 15 5.441350416241161 6 16 7.217152499061822 6 17 7.761954870097341 6 18 8.033049223559729 6 19 7.022448996543925 6 20 6.046802939931776 6 21 6.341974855990191 6 22 7.196602261635952 6 23 6.967371646566658 6 24 7.138312960701423 6 25 6.511761282710577 6 26 5.6681856078755 6 27 5.7996385082196085 6 28 5.256717792437918 6 29 5.380689148370227 6 30 5.007117654854785 6 31 4.719194518339144 6 32 4.548804680270507 6 33 3.742525193592609 6 34 3.900009042744177 6 35 5.1309945475268215 6 36 5.331141385475579 6 37 5.996686768670433 6 38 7.449879032471479 6 39 7.976233152831689 6 40 4.243811278023461 6 41 11.797867898258282 6 42 11.121291472099202 6 43 11.070547165107513 6 44 12.032411536148576 6 45 10.910139750342413 6 46 12.764306699719072 6 47 13.426724170876277 6 48 13.17565194546603 6 49 13.41987264256134 6 50 13.566634217639198 6 51 12.506791032026051 6 52 11.437254806029308 6 53 11.973289556094091 6 54 12.52946394657775 6 55 11.595240396214049 6 56 10.904664617409331 7 1 3.5145863164494457 7 2 6.67601202800105 7 3 4.742191335092173 7 4 4.689805521131554 7 5 7.881783804418131 7 6 4.740139507622793 7 7 4.120854544367184 7 8 3.9209997907016074 7 9 4.380992825764849 7 10 5.2222676616595365 7 11 4.645057431459136 7 12 4.987722563438055 7 13 5.6726068521894355 7 14 4.9474603762363785 7 15 5.419611285806379 7 16 6.7606307599313915 7 17 8.435867913575606 7 18 8.815657919211901 7 19 7.89201421393523 7 20 6.3946290268883 7 21 7.1898009429467145 7 22 7.740080522505522 7 23 6.663023820479697 7 24 7.116573830266642 7 25 6.924804760971451 7 26 5.8420986513537585 7 27 4.669203725610913 7 28 5.908891705481395 7 29 5.424167409239789 7 30 5.507117654854785 7 31 5.436585822686972 7 32 5.853152506357468 7 33 5.720786063157828 7 34 5.052182955787654 7 35 4.870124982309431 7 36 6.287663124606016 7 37 6.561904159974784 7 38 7.275965988993221 7 39 8.28058097891865 7 40 8.135115625849547 7 41 12.667433115649587 7 42 12.490856689490506 7 43 11.222721078150997 7 44 12.662846318757271 7 45 10.82318322860328 7 46 12.916480612762548 7 47 13.970202431745847 7 48 13.240869336770373 7 49 11.985090033865685 7 50 12.870982043726151 7 51 11.702443205939097 7 52 12.372037414724964 7 53 11.842854773485396 7 54 11.55120307701253 7 55 11.182196917953181 7 56 11.426403747844116 8 1 3.4529078533554127 8 2 6.495526689275064 8 3 4.645123589895 8 4 4.559876299695766 8 5 7.052663480858985 8 6 3.6951445632345212 8 7 3.6789940792509057 8 8 3.369938112238515 8 9 4.020022148312876 8 10 4.148455730415854 8 11 3.576301111944474 8 12 4.0317063854805255 8 13 5.333880866345144 8 14 4.96667170080665 8 15 5.061673975391816 8 16 6.4062323777271395 8 17 6.767516042999262 8 18 7.016365704853968 8 19 6.8581416153507995 8 20 5.984618915664836 8 21 6.907192247294542 8 22 7.0237003405034955 8 23 5.973438380641479 8 24 5.883004568385953 8 25 5.703368967240404 8 26 5.332998550241527 8 27 5.400245181627092 8 28 4.157122241376236 8 29 5.2290207964996505 8 30 5.5789073414068575 8 31 4.66661615635735 8 32 5.207045327388812 8 33 3.431605072257927 8 34 4.736207222723959 8 35 5.021287773007106 8 36 4.960565045738473 8 37 4.582126606890856 8 38 5.848261236718194 8 39 6.661268542113795 8 40 0.3469457572954511 8 41 10.902519061048977 8 42 10.83666053175542 8 43 7.768727144885069 8 44 8.837264923408437 8 45 8.516813157824718 8 46 10.530737437838383 8 47 10.29275046005727 8 48 10.549767213413446 8 49 10.994695696150824 8 50 10.384126634221602 8 51 10.019935622521501 8 52 9.119762389446905 8 53 8.16540280179682 8 54 8.894984674585839 8 55 9.339426442725678 8 56 7.1387394404629205 9 1 3.493882382702033 9 2 6.191539978311614 9 3 4.704924254346828 9 4 3.623552933139841 9 5 7.813460823051674 9 6 4.0755432343308655 9 7 3.8113307348433736 9 8 3.0814552772440535 9 9 3.7215725359097718 9 10 2.9707148666284766 9 11 2.392469439740708 9 12 4.35728778082936 9 13 5.289584077862312 9 14 4.267336152633895 9 15 6.121474639843644 9 16 6.6022456667636895 9 17 7.658435201360284 9 18 7.843608229770908 9 19 5.806092888883466 9 20 5.557154906805483 9 21 5.097668437770732 9 22 6.532005988344025 9 23 5.16612941054181 9 24 5.226304679127924 9 25 5.693955899687804 9 26 4.924914386343403 9 27 3.0957047608075996 9 28 3.374730214798163 9 29 5.411745048991339 9 30 4.478132147608402 9 31 3.377579611506846 9 32 3.304498262051048 9 33 3.9133326470087617 9 34 2.93831132017689 9 35 3.036791648976095 9 36 4.452259397897944 9 37 5.129191944653872 9 38 5.454019819220967 9 39 5.443106858835833 9 40 4.3369789798868155 9 41 7.821677422067808 9 42 9.391477807502923 9 43 9.444253169248306 9 44 11.11522727113822 9 45 9.143059005000797 9 46 11.32641850096131 9 47 9.989871168805884 9 48 10.099047390600603 9 49 9.823599350635874 9 50 9.373052437100895 9 51 9.127909044448412 9 52 10.464169919900947 9 53 10.195856843878769 9 54 11.17128589274752 9 55 10.214288015261669 9 56 10.311496915545977 10 1 3.64106852988818 10 2 6.87660491337655 10 3 4.610768410190985 10 4 4.476366785953694 10 5 7.553720563311412 10 6 3.4002185590061913 10 7 4.007218180730817 10 8 3.577126272915052 10 9 3.9185422328794743 10 10 4.772662918576529 10 11 4.0699586172298865 10 12 3.243651417192993 10 13 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-5.164239645870641 114 16 -4.504897190379168 114 17 -0.5439457510206687 114 18 0.09360822977091132 114 19 -0.5986690158784356 114 20 -0.5261784265278528 114 21 4.407192247294542 114 22 5.424863131201171 114 23 4.344700839113241 114 24 5.714399917223165 114 25 4.848717804449709 114 26 4.186819148248169 114 27 4.33379999890284 114 28 3.6604445005124546 114 29 4.876030763277054 114 30 4.597179766656023 114 31 3.9132938972211306 114 32 4.828307785860574 114 33 3.1633326470087617 114 34 3.4621208439864155 114 35 4.120124982309431 114 36 5.166545112183652 114 37 5.7482395637014925 114 38 6.084972200173347 114 39 8.288344954073928 114 40 10.194121837029673 114 41 10.405010755401136 114 42 10.141477807502923 114 43 8.253776978772109 114 44 -0.801439395528444 114 45 6.893059005000794 114 46 -1.2807243561815476 114 47 -5.022033593098875 114 48 0.12285691441012503 114 49 -7.283543506506982 114 50 0.6706714847199429 114 51 3.7350519015912695 114 52 2.8332175389485634 114 53 3.6363330343549585 114 54 3.0046192260808553 114 55 3.809526110499764 114 56 5.13292548697455 115 1 3.053406192225843 115 2 4.941539978311614 115 3 3.954924254346828 115 4 3.778314837901746 115 5 5.670603680194528 115 6 3.5755432343308655 115 7 3.335140258652899 115 8 3.450502896291674 115 9 3.626334440671677 115 10 4.6135720094856225 115 11 2.832945630216898 115 12 -0.10699793345635555 115 13 -0.2699397316614984 115 14 -1.9826638473661085 115 15 -4.057096788727783 115 16 1.923674238192259 115 17 2.813197106122189 115 18 3.950751086913769 115 19 3.4727595555501374 115 20 3.473821573472147 115 21 5.871477961580258 115 22 5.567720274058317 115 23 5.773272267684671 115 24 6.6786856315088805 115 25 5.634432090163994 115 26 5.901104862533884 115 27 5.476657141759979 115 28 5.160444500512455 115 29 4.304602191848485 115 30 4.954322623798884 115 31 4.341865325792561 115 32 4.328307785860574 115 33 5.091904075580192 115 34 4.854977986843554 115 35 5.298696410880861 115 36 6.916545112183652 115 37 6.391096706558631 115 38 8.227829343030486 115 39 8.395487811216782 115 40 10.979836122743958 115 41 11.190725041115428 115 42 9.927192093217215 115 43 9.753776978772109 115 44 2.8057034616144136 115 45 8.107344719286512 115 46 3.6835613581041677 115 47 -6.486319307384591 115 48 3.5871426286958403 115 49 -3.1406863636498414 115 50 3.777814341862797 115 51 6.127909044448412 115 52 5.690360396091421 115 53 6.850618748640674 115 54 6.218904940366567 115 55 5.809526110499764 115 56 9.097211201260265 116 1 2.7056618313235887 116 2 4.920863286582286 116 3 3.2124430513393136 116 4 2.7801945371498604 116 5 5.283385635081746 116 6 3.507874061398539 116 7 2.6471703338408688 116 8 3.1177961293743763 116 9 2.791747974506265 116 10 3.1135720094856225 116 11 3.1393366076605105 116 12 2.5678140966188323 116 13 4.168030193150532 116 14 3.3650805135361495 116 15 -5.002585510532295 116 16 2.9105163434554164 116 17 5.506806128678576 116 18 4.1669165004475985 116 19 4.702082863820809 116 20 4.22758097196839 116 21 4.565086984136645 116 22 4.9117052364643286 116 23 4.590941440616998 116 24 4.727557811960008 116 25 3.9408230676076066 116 26 4.367270276067714 116 27 4.954100750782537 116 28 4.596534726076364 116 29 3.5546021918484847 116 30 3.9693602177838407 116 31 3.990361566394064 116 32 4.403495755785386 116 33 3.014836406407259 116 34 3.5899403928585976 116 35 4.0280197191515335 116 36 4.335718044514483 116 37 4.421171894528559 116 38 5.064295508444019 116 39 6.903006608209267 116 40 7.660287250563503 116 41 5.5873415824688095 116 42 4.455387581939018 116 43 1.2124235953134637 116 44 0.13653052928358633 116 45 4.10922441853463 116 46 -0.1510251080612477 116 47 -6.486319307384591 116 48 -0.5989475968680686 116 49 -1.4245209501160048 116 50 1.6387165975018974 116 51 5.0113676910649545 116 52 4.233593478798188 116 53 4.938964613302328 116 54 5.079807196005667 116 55 3.9899772383193124 116 56 6.988188644869286 117 1 2.8569776207972737 117 2 5.179635216406847 117 3 4.133495682918259 117 4 3.9271243617112646 117 5 6.640841775432623 117 6 3.6350670438546757 117 7 3.037521211033848 117 8 3.5695505153392943 117 9 3.388239202576443 117 10 4.655238676152287 117 11 1.3388980111692774 117 12 -4.64271221917064 117 13 -4.388987350709115 117 14 -0.7445686092708677 117 15 -8.199953931584925 117 16 -8.242992428474409 117 17 -0.026088608163526317 117 18 -2.7694870083243295 117 19 -0.03914520635462537 117 20 -0.6035593789088054 117 21 5.032192247294542 117 22 5.216529797867835 117 23 4.338748458160861 117 24 5.0060665838898295 117 25 5.182051137783045 117 26 4.889200100629118 117 27 4.1909428560456945 117 28 3.684254024321973 117 29 4.929602191848485 117 30 4.394798814275074 117 31 4.389484373411612 117 32 3.8640220715748583 117 33 3.9490469327230464 117 34 5.081168463034036 117 35 2.620124982309431 117 36 5.976068921707462 117 37 5.438715754177682 117 38 6.114734104935252 117 39 7.1990592397882125 117 40 10.348883741791578 117 41 7.006201231591614 117 42 5.492668283693401 117 43 -4.811699211704077 117 44 -1.7121536812427287 117 45 5.8394875764293666 117 46 0.9037994533422626 117 47 0.34701402594874153 117 48 -0.10333356178034947 117 49 -1.1942577922212685 117 50 2.6409095799580378 117 51 6.8600519015912695 117 52 7.279646110377136 117 53 6.427999701021626 117 54 7.248666845128472 117 55 6.678573729547381 117 56 7.549592153641218 118 1 2.3406732729711877 118 2 5.262968549740183 118 3 3.7421913350921727 118 4 3.320240303740249 118 5 6.099175108765959 118 6 3.2836177684923626 118 7 2.8165067182802304 118 8 3.0949128341798726 118 9 3.076644999677889 118 10 4.091832879050841 118 11 -0.0940730033234729 118 12 -13.38184265395325 118 13 -10.84913227824535 118 14 -10.87862658028536 118 15 -9.276040888106664 118 16 -7.782847500938175 118 17 -1.0858712168591786 118 18 -7.684342080788095 118 19 -2.216681438238684 118 20 -2.279284016589962 118 21 3.6898009429467145 118 22 5.283558783375085 118 23 3.4456325161318766 118 24 4.4209216563536025 118 25 2.794369978362756 118 26 4.037750825266805 118 27 3.0170298125674364 118 28 3.2349786620031367 118 29 3.858950017935438 118 30 2.5071176548547847 118 31 2.6322379966000184 118 32 2.1140220715748583 118 33 2.3294817153317418 118 34 3.726095999265919 118 35 1.717951069265954 118 36 4.439837037649493 118 37 2.692338942583479 118 38 3.623792075949744 118 39 5.6066679354403846 118 40 6.635115625849547 118 41 4.8196070286930635 118 42 3.44737842862094 118 43 -1.9511919653272685 118 44 -0.31541455080794734 118 45 3.736226706864148 118 46 -2.9313454741939715 118 47 -1.203710611732415 118 48 -2.2156524023600603 118 49 -3.471431705264745 118 50 -1.1942353475781964 118 51 4.3980953798521405 118 52 3.6546461103771364 118 53 3.668941730007134 118 54 4.594681337882097 118 55 4.203936048387963 118 56 5.491621139148464 119 1 2.2676919065115584 119 2 5.370111406883037 119 3 3.0620671114896894 119 4 3.2426005521874544 119 5 6.349175108765959 119 6 3.0755432343308655 119 7 2.9065688300814685 119 8 2.5933600391488127 119 9 2.876334440671677 119 10 3.1135720094856225 119 11 -0.27419722692595627 119 12 -9.39271221917064 119 13 -4.841368303090068 119 14 -5.4826638473661085 119 15 -8.521382503013498 119 16 -8.540611476093456 119 17 -1.5796600367349534 119 18 -5.442106055943377 119 19 -0.9915261587355779 119 20 -1.7761784265278528 119 21 4.371477961580258 119 22 3.1034345597726016 119 23 4.023272267684671 119 24 3.75011420293745 119 25 2.3487178044497092 119 26 3.972533433962454 119 27 3.619514284617125 119 28 2.231873071941024 119 29 3.7331736204199153 119 30 4.275751195227453 119 31 3.020436754363992 119 32 3.2568792144320042 119 33 3.1633326470087617 119 34 3.283549415414985 119 35 3.1558392680237155 119 36 4.130830826469367 119 37 4.033953849415777 119 38 4.442115057316201 119 39 5.466916382645358 119 40 7.0155504084582425 119 41 6.012153612543997 119 42 4.712906378931496 119 43 -5.674794449799318 119 44 -4.337153681242729 119 45 4.9644875764293666 119 46 -0.030724356181547563 119 47 -2.0934621645274483 119 48 -2.8414287998755903 119 49 -4.104972077935553 119 50 -1.5793285152800571 119 51 3.627909044448412 119 52 4.368931824662852 119 53 3.9577616057835314 119 54 2.8260477975094247 119 55 4.023811824785476 119 56 6.311496915545977 120 1 2.6974948621765833 120 2 5.952623722153977 120 3 3.0990129242975684 120 4 3.5406301088377035 120 5 6.9957268329038875 120 6 3.2011589978776698 120 7 2.9829235098844222 120 8 3.348286147523197 120 9 3.313526558898282 120 10 4.096330630175281 120 11 0.6765416893302003 120 12 -5.953057046756847 120 13 -5.219447120824061 120 14 -3.488821482834087 120 15 -0.4154711729642351 120 16 -7.895291279049122 120 17 0.1420148401123349 120 18 -5.141613445105939 120 19 0.009705368358016386 120 20 0.14500383948200124 120 21 4.32098535074282 120 22 6.088656234649449 120 23 5.156277193793041 120 24 4.567848193085236 120 25 4.857338494104884 120 26 4.472533433962454 120 27 3.5702532008732817 120 28 4.0865528748966895 120 29 4.520119433227794 120 30 4.914913756803809 120 31 3.3578751780093086 120 32 4.320918623298994 120 33 3.268012449964427 120 34 3.765076509011042 120 35 3.6115042926542564 120 36 4.412850530902869 120 37 5.674347938085727 120 38 6.578814564705361 120 39 6.720610963926141 120 40 7.800033167078933 120 41 -4.3917872741555115 120 42 -0.844975394467518 120 43 -3.706814154232813 120 44 -12.871636439863419 120 45 -0.13034000977753024 120 46 -6.198212040910612 120 47 -6.607008962557005 120 48 -2.623448504309085 120 49 -13.164085378428165 120 50 -1.7024812246396657 120 51 4.105741556763682 120 52 4.930508179342652 120 53 5.817367517113578 120 54 5.738609373864104 120 55 4.526274878972668 120 56 9.84844272835386 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 662.0 5 14.0 6 60.0 7 60.0 8 101.0 9 93.0 10 88.0 11 63.0 12 84.0 13 68.0 14 90.0 15 89.0 16 137.0 17 157.0 18 192.0 19 260.0 20 309.0 21 479.0 22 566.0 23 697.0 24 770.0 25 910.0 26 1002.0 27 1024.0 28 1012.0 29 1120.0 30 1209.0 31 1409.0 32 1565.0 33 1727.0 34 1839.0 35 2025.0 36 2279.0 37 2665.0 38 2692.0 39 2143.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 100.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.409979770735 0.4652730950775455 0.10114632501685772 0.023600809170600135 8 99.49089683074848 0.31692515171948754 0.06743088334457181 0.12474713418745785 9 98.2434254888739 0.6810519217801754 0.6271072151045178 0.44841537424140254 10 53.833445718138904 24.52124072825354 13.22319622387053 8.42211732973702 11 30.832771409305458 26.40593391773432 19.231287929871883 23.530006743088336 12 26.557653405259607 21.901550910316924 24.150370869858396 27.390424814565073 13 21.41604855023601 18.11530681051922 25.694538098449087 34.774106540795685 14 20.772083614295347 26.54753877275792 28.401888064733647 24.27848954821308 15 27.89941028943266 18.664000542262592 26.265844235070833 27.17074493323392 16 25.744644965424186 25.326361949738573 24.830494181143532 24.098498903693706 17 25.195602320248213 20.359503574801025 31.242411978955886 23.202482125994877 18 23.75252865812542 20.276466621712743 24.356035064059338 31.61496965610249 19 25.65944815489442 20.87634082169601 23.234163124873508 30.23004789853606 20 24.824395515331624 22.92989328650547 29.704173983520192 22.541537214642712 21 24.831422791638573 19.217801753202966 26.881321645313555 29.06945380984491 22 24.082939986513825 19.565070802427513 30.434929197572487 25.917060013486175 23 22.646662171274443 23.08496291301416 28.516520566419416 25.751854349291975 24 21.686503253649818 23.02167975993796 25.26046056846151 30.031356417950704 25 24.436052196783223 24.10223555990154 28.033853727619114 23.427858515696126 26 31.26454615981381 23.402030559584443 21.131986372988834 24.201436907612912 27 24.342926549478726 27.139917001248353 21.927190525996153 26.589965923276765 28 27.790140940049902 26.650482163328615 21.63665790006069 23.922718996560793 29 29.10926526202053 25.597649918962723 22.25486223662885 23.0382225823879 30 27.18685693080997 22.28519380629491 28.117936781027563 22.410012481867557 31 28.773314445681137 26.948632331613208 22.273938412762657 22.004114809942998 32 30.468802698145026 25.62900505902192 22.070826306913997 21.831365935919056 33 29.71266502346625 24.69865280075632 22.27099301077084 23.317689165006584 34 24.37590784717765 24.75086984427254 29.321352565618348 21.55186974293146 35 23.871272751831967 24.354168777226217 31.10458244689832 20.669976024043493 36 26.509682207678292 24.816139261858176 26.30726671614601 22.366911814317522 37 27.486999392179374 24.20814479638009 26.217329641385835 22.0875261700547 38 29.736397431564715 23.284893545116596 25.035484961135516 21.94322406218317 39 27.939885173927724 22.796352583586625 23.059777102330294 26.20398514015535 40 23.301928505833136 18.812285296418487 24.591000306112036 33.294785891636344 41 19.15734933187892 16.829151895282244 23.138805563130624 40.874693209708205 42 23.912896463109487 25.333062825454792 24.846148644079257 25.907892067356464 43 20.599276269065577 23.3048124725219 27.724982244918667 28.37092901349386 44 20.32859078590786 25.05758807588076 27.137533875338754 27.476287262872628 45 24.6307250295758 23.413892175088726 28.13249957748859 23.822883217846883 46 22.71435338702037 25.231779116194087 25.465250050754552 26.588617446030995 47 19.149008306492625 23.753178504831325 23.84810984912697 33.24970333954907 48 25.552593392501606 25.08014713326359 26.062160429251175 23.30509904498363 49 21.795868690625106 24.145508637884987 25.149599377937047 28.90902329355286 50 21.888092985538588 26.736721178537643 25.794026219759424 25.581159616164346 51 22.016920473773265 28.426395939086298 25.42808798646362 24.12859560067682 52 22.68211920529801 29.189755372347616 26.51371807000946 21.614407352344912 53 21.959139493979162 28.670004058990667 26.535651468001625 22.835204979028546 54 21.618422832409035 25.409170837278506 28.32070877857433 24.65169755173813 55 22.277478940424235 24.56781352549139 27.82232145877736 25.332386075307014 56 23.240008115236357 23.94332859944546 28.82599580712788 23.990667478190304 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.0 7 2.0 8 13.0 9 24.0 10 35.5 11 47.0 12 62.0 13 68.5 14 60.0 15 59.0 16 58.0 17 63.0 18 68.0 19 76.0 20 84.0 21 95.0 22 100.5 23 95.0 24 131.0 25 167.0 26 171.0 27 175.0 28 188.5 29 223.5 30 245.0 31 290.5 32 336.0 33 427.0 34 518.0 35 579.0 36 640.0 37 650.0 38 699.5 39 739.0 40 813.0 41 887.0 42 1067.0 43 1247.0 44 1555.5 45 1864.0 46 1898.5 47 1743.5 48 1554.0 49 1573.0 50 1592.0 51 1685.0 52 1778.0 53 1768.0 54 1764.5 55 1771.0 56 1826.5 57 1882.0 58 1970.5 59 2059.0 60 1944.0 61 1829.0 62 1651.0 63 1325.5 64 1178.0 65 1018.0 66 858.0 67 715.5 68 573.0 69 505.5 70 438.0 71 365.5 72 257.0 73 221.0 74 154.0 75 87.0 76 64.5 77 42.0 78 35.0 79 18.5 80 9.0 81 5.5 82 2.0 83 1.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.519217801753203 16 0.05057316250842886 17 0.026972353337828728 18 0.0 19 0.04720161834120027 20 0.16183412002697234 21 0.0 22 0.0 23 0.0 24 0.003371544167228591 25 0.010114632501685771 26 0.043830074173971674 27 0.07080242751180041 28 0.006743088334457182 29 0.14834794335805798 30 0.057316250842886045 31 0.037086985839514496 32 0.033715441672285906 33 0.14497639919082939 34 0.19217801753202968 35 0.15846257585974377 36 0.060687795010114634 37 0.15509103169251517 38 0.23600809170600134 39 0.16857720836142953 40 0.8732299393122049 41 1.0923803101820635 42 0.28995279838165877 43 0.3068105192178018 44 0.4720161834120027 45 0.2528658125421443 46 0.3573836817262306 47 0.5563047875927174 48 0.09103169251517194 49 0.2730950775455159 50 0.21577882670262982 51 0.37086985839514497 52 0.21577882670262982 53 0.3236682400539447 54 0.29669588671611596 55 0.3405259608900877 56 0.28995279838165877 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 29660.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.00202292650033 #Duplication Level Percentage of deduplicated Percentage of total 1 89.25827927620348 70.51584625758598 2 6.845339706384432 10.815913688469319 3 1.8777739842949812 4.450438300741739 4 0.699897575964493 2.2117329737019555 5 0.3926254694434961 1.5509103169251517 6 0.2389894161829976 1.1328388401888065 7 0.1280300443837487 0.708024275118004 8 0.11095937179924889 0.7012811867835468 9 0.06828269033799932 0.4855023600809171 >10 0.34568111983612154 4.4335805799055965 >50 0.021338340730624787 1.0182063385030344 >100 0.012803004438374871 1.9757248819959543 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TAGCATGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT 226 0.7619689817936615 No Hit TAGCATGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC 193 0.6507080242751181 No Hit TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 167 0.5630478759271746 No Hit TAGCATGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA 73 0.2461227242076871 No Hit TAGCATGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT 60 0.20229265003371544 No Hit TAGCATGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC 58 0.19554956169925827 No Hit TAGCATGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT 56 0.18880647336480108 No Hit TAGCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 55 0.18543492919757248 No Hit TAGCATGGGACTAATCCTAGCCCTAGCCCTACACAAATATCATTATACTATTATAT 47 0.15846257585974377 No Hit TAGCATGGGGAGCTCCATTATGCTTTGGCAGGTGATCAGACTGAAGCGTAAATGGA 40 0.13486176668914363 No Hit TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 36 0.12137559002022927 No Hit TAGCATGGGGATCCCCTCGACCACTCTTTTGGCGCTTCGCTGTCGACAGTGCGCTT 34 0.11463250168577209 No Hit TAGCATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA 32 0.10788941335131491 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0033715441672285905 0.0 0.0 0.0 0.0 11 0.010114632501685773 0.0 0.0 0.0 0.0 12 0.010114632501685773 0.0 0.0 0.0 0.0 13 0.010114632501685773 0.0 0.0 0.0 0.0 14 0.016857720836142953 0.0 0.0 0.0 0.0 15 0.020229265003371546 0.0 0.0 0.0 0.0 16 0.023600809170600135 0.0 0.0 0.0 0.0 17 0.030343897505057317 0.0 0.0 0.0 0.0 18 0.033715441672285906 0.0 0.0 0.0 0.0 19 0.04045853000674309 0.0 0.0 0.0 0.0 20 0.04045853000674309 0.0 0.0 0.0 0.0 21 0.04045853000674309 0.0 0.0 0.0 0.0 22 0.04383007417397168 0.0 0.0 0.0 0.0 23 0.04720161834120027 0.0 0.0 0.0 0.0 24 0.05057316250842886 0.0 0.0 0.0 0.0 25 0.05057316250842886 0.0 0.0 0.0 0.0 26 0.05057316250842886 0.0 0.0 0.0 0.0 27 0.060687795010114634 0.0 0.0 0.0 0.0 28 0.06743088334457181 0.0 0.0 0.0 0.0 29 0.07080242751180041 0.0 0.0 0.0 0.0 30 0.07417397167902899 0.0 0.0 0.0 0.0 31 0.08091706001348618 0.0 0.0 0.0 0.0 32 0.08091706001348618 0.0 0.0 0.0 0.0 33 0.08091706001348618 0.0 0.0 0.0 0.0 34 0.08091706001348618 0.0 0.0 0.0 0.0 35 0.08428860418071477 0.0 0.0 0.0 0.0 36 0.08766014834794336 0.0 0.0 0.0 0.0 37 0.09103169251517194 0.0 0.0 0.0 0.0 38 0.09777478084962914 0.0 0.0 0.0 0.0 39 0.09777478084962914 0.0 0.0 0.0 0.0 40 0.10114632501685772 0.0 0.0 0.0 0.0 41 0.10114632501685772 0.0 0.0 0.0 0.0 42 0.1078894133513149 0.0 0.0 0.0 0.0 43 0.1112609575185435 0.0 0.0 0.0 0.0 44 0.11463250168577209 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAATT 15 0.007866333 50.759037 38 ATAATTA 15 0.007866333 50.759037 39 TAATTAT 15 0.007866333 50.759037 40 GCGCATT 30 9.127525E-7 50.759037 38 CGCATTG 30 9.127525E-7 50.759037 39 ACTGACT 15 0.007920128 50.67182 41 AATTATA 15 0.007920128 50.67182 41 CATTGAA 20 3.8541263E-4 50.671818 41 CGTCTCA 20 3.8541263E-4 50.671818 41 GGCGCAT 30 9.3475865E-7 50.584908 37 TGCTCGT 15 0.007974196 50.584908 37 AATACCA 15 0.007974196 50.584908 37 TATGCTC 15 0.008028539 50.49829 35 AGAGCGC 15 0.008028539 50.49829 35 AATATAA 15 0.008028539 50.49829 36 ATCCTAG 25 2.0008696E-5 50.154762 14 ACTAATC 25 2.0008696E-5 50.154762 10 TGGTTGA 40 2.4629117E-9 50.154762 14 AATCCTA 25 2.0008696E-5 50.154762 13 GGTTGAT 40 2.4629117E-9 50.154762 15 >>END_MODULE