FastQCFastQC Report
Fri 17 Jun 2016
SRR1526009_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526009_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4175
Sequences flagged as poor quality0
Sequence length40
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG190.45508982035928147No Hit
TAGCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC140.33532934131736525No Hit
TAGCATGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCG100.23952095808383234No Hit
TAGCATGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCC70.16766467065868262No Hit
TAGCATGGGGATCCAGTGACAGATACCATTAGATTCAAGA60.1437125748502994No Hit
TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT60.1437125748502994No Hit
TAGCATGGGAACTAGGGTTCTACCAGGGAGACAAGAGTCT50.11976047904191617No Hit
TAGCATGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT50.11976047904191617No Hit
TAGCATGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT50.11976047904191617No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGC357.2492E-734.329275
ATGGGGA404.816684E-834.329275
ATGGGAT404.816684E-834.329275
ATGGGAG404.816684E-834.329275
ATGGGAC404.816684E-834.329275
ATGGGAA453.1723175E-934.329275
GAAGCAG200.002364214534.329279
TGGGGGG357.2492E-734.329276
TGGGGCT200.002364214534.329276
GGGGGGC251.6047586E-434.329277
GCATGGG3750.034.329273
CATGGGT200.002364214534.329274
CATGGGG1650.034.329274
CATGGGC251.6047586E-434.329274
CATGGGA1650.034.329274
TGGGATC200.002364214534.329276
TGGGAGG200.002364214534.329276
ATGGGGT301.0823749E-534.3292665
ATGGGGG600.034.3292665
GGAAGCA301.0823749E-534.3292668
Produced by FastQC (version 0.11.5)