FastQCFastQC Report
Fri 17 Jun 2016
SRR1526008_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526008_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1090
Sequences flagged as poor quality0
Sequence length40
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCATGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT50.45871559633027525No Hit
TAGCATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT40.3669724770642202No Hit
TAGCATGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.3669724770642202No Hit
TAGCATGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.27522935779816515No Hit
TAGCATGGGGAGTCGCCTTTTTTTTTTTTTTTTTTTTTTT20.1834862385321101No Hit
TAGCATGGGTCGTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1834862385321101No Hit
TAGCATGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTCTT20.1834862385321101No Hit
TAGCATGGGGGGGCTGGTGAGATGGCCCAGTGGGTAAGAG20.1834862385321101No Hit
TAGCATGGGGGGCTGGTGCGATGCCCCAGTGGGTAAGAGC20.1834862385321101No Hit
TAGCATGGGAATGGTTCAACGCAGAGCCGACTTTTTTTTT20.1834862385321101No Hit
TAGCATGGGTGGTGCTTTTGTATTGGTATTGTATGATGAG20.1834862385321101No Hit
TAGCATGGGGCGTCGCCTTTTTTTTTTTTTTTTTTTTTTT20.1834862385321101No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGAG200.001707216534.05
TGGGGGG200.001707216534.06
CATGGGA259.5802614E-534.04
TAGCATG1100.032.4545441
AGCATGG1100.032.4545442
GCATGGG1100.030.909093
CATGGGG453.3740434E-830.2222214
ATGGGGG407.271574E-729.755
CATGGGC250.00511333727.1999994
Produced by FastQC (version 0.11.5)