##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526008_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1090 Sequences flagged as poor quality 0 Sequence length 40 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 17.223853211009175 22.0 4.0 29.0 4.0 32.0 2 11.256880733944953 4.0 4.0 20.0 4.0 26.0 3 9.992660550458716 4.0 4.0 16.0 4.0 22.0 4 8.512844036697247 4.0 4.0 11.0 4.0 15.0 5 10.877981651376146 4.0 4.0 19.0 4.0 25.0 6 11.919266055045872 4.0 4.0 22.0 4.0 26.0 7 12.305504587155964 4.0 4.0 22.0 4.0 27.0 8 12.902752293577981 4.0 4.0 26.0 4.0 29.0 9 11.921100917431193 4.0 4.0 23.0 4.0 27.0 10 9.320183486238532 4.0 4.0 15.0 4.0 22.0 11 11.971559633027523 4.0 4.0 19.0 4.0 33.0 12 11.974311926605505 4.0 4.0 21.0 4.0 33.0 13 11.837614678899083 4.0 4.0 20.0 4.0 32.0 14 11.626605504587156 4.0 4.0 20.0 4.0 33.0 15 11.288073394495413 4.0 4.0 19.0 4.0 33.0 16 8.754128440366973 4.0 4.0 10.0 4.0 24.0 17 8.256880733944953 4.0 4.0 6.0 4.0 23.0 18 7.9 4.0 4.0 6.0 4.0 20.0 19 8.089908256880735 4.0 4.0 4.0 4.0 22.0 20 8.01651376146789 4.0 4.0 4.0 4.0 22.0 21 8.692660550458715 4.0 4.0 4.0 4.0 28.0 22 8.573394495412844 4.0 4.0 4.0 4.0 26.0 23 8.529357798165137 4.0 4.0 4.0 4.0 26.0 24 8.293577981651376 4.0 4.0 4.0 4.0 25.0 25 8.402752293577981 4.0 4.0 4.0 4.0 27.0 26 8.796330275229359 4.0 4.0 4.0 4.0 31.0 27 8.720183486238533 4.0 4.0 4.0 4.0 30.0 28 8.767889908256882 4.0 4.0 4.0 4.0 30.0 29 8.752293577981652 4.0 4.0 4.0 4.0 29.0 30 8.839449541284404 4.0 4.0 4.0 4.0 31.0 31 9.103669724770642 4.0 4.0 4.0 4.0 35.0 32 8.965137614678898 4.0 4.0 4.0 4.0 34.0 33 8.970642201834863 4.0 4.0 4.0 4.0 35.0 34 9.071559633027523 4.0 4.0 4.0 4.0 35.0 35 8.839449541284404 4.0 4.0 4.0 4.0 35.0 36 8.461467889908256 4.0 4.0 4.0 4.0 31.0 37 8.314678899082569 4.0 4.0 4.0 4.0 31.0 38 8.242201834862385 4.0 4.0 4.0 4.0 31.0 39 8.279816513761467 4.0 4.0 4.0 4.0 31.0 40 8.336697247706422 4.0 4.0 4.0 4.0 31.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 6.3382738095238125 1 2 -5.746845238095241 1 3 -3.888154761904759 1 4 -3.257142857142858 1 5 -5.392321428571429 1 6 -10.037083333333332 1 7 -10.793214285714289 1 8 -11.561488095238095 1 9 -10.245416666666666 1 10 -7.236309523809524 1 11 -8.628511904761904 1 12 -8.962440476190476 1 13 -8.567499999999999 1 14 -8.782142857142857 1 15 -8.39232142857143 1 16 -6.32375 1 17 -5.355416666666667 1 18 -4.915357142857141 1 19 -5.4663095238095245 1 20 -5.305119047619048 1 21 -6.779166666666667 1 22 -6.354642857142856 1 23 -6.528095238095238 1 24 -5.796250000000001 1 25 -6.741428571428571 1 26 -7.034047619047618 1 27 -6.959642857142857 1 28 -6.463154761904759 1 29 -7.0006547619047605 1 30 -6.984285714285715 1 31 -8.15202380952381 1 32 -7.335357142857143 1 33 -6.80827380952381 1 34 -7.3225 1 35 -6.9278571428571425 1 36 -6.3129761904761885 1 37 -6.550119047619045 1 38 -6.583749999999998 1 39 -6.210119047619047 1 40 -6.4016071428571415 7 1 3.5882738095238125 7 2 0.0864880952380922 7 3 -1.221488095238092 7 4 18.74285714285714 7 5 -2.558988095238096 7 6 -6.037083333333332 7 7 3.2067857142857115 7 8 3.4385119047619046 7 9 0.7545833333333345 7 10 12.763690476190476 7 11 17.371488095238096 7 12 17.037559523809524 7 13 6.432500000000001 7 14 17.21785714285714 7 15 20.607678571428572 7 16 -6.32375 7 17 -5.355416666666667 7 18 -4.915357142857141 7 19 -5.4663095238095245 7 20 -5.305119047619048 7 21 -6.779166666666667 7 22 -6.354642857142856 7 23 -6.528095238095238 7 24 -5.796250000000001 7 25 -6.741428571428571 7 26 -7.034047619047618 7 27 -6.959642857142857 7 28 -6.463154761904759 7 29 -7.0006547619047605 7 30 -6.984285714285715 7 31 -8.15202380952381 7 32 -7.335357142857143 7 33 -6.80827380952381 7 34 -7.3225 7 35 -6.9278571428571425 7 36 -6.3129761904761885 7 37 -6.550119047619045 7 38 -6.583749999999998 7 39 -6.210119047619047 7 40 -6.4016071428571415 9 1 -0.24505952380951967 9 2 -1.4135119047619078 9 3 5.61184523809524 9 4 -0.9238095238095241 9 5 -1.558988095238096 9 6 5.962916666666668 9 7 3.7067857142857115 9 8 2.4385119047619046 9 9 2.7545833333333345 9 10 0.430357142857142 9 11 3.20482142857143 9 12 -1.1291071428571424 9 13 1.099166666666667 9 14 3.2178571428571434 9 15 1.2743452380952363 9 16 0.6762499999999996 9 17 2.477916666666667 9 18 0.5846428571428586 9 19 1.0336904761904755 9 20 2.528214285714286 9 21 2.3874999999999993 9 22 5.4786904761904776 9 23 5.805238095238094 9 24 4.703749999999999 9 25 5.091904761904763 9 26 3.4659523809523822 9 27 2.540357142857143 9 28 3.203511904761907 9 29 2.3326785714285734 9 30 2.682380952380951 9 31 2.1813095238095244 9 32 2.997976190476191 9 33 3.5250595238095244 9 34 2.510833333333334 9 35 3.4054761904761914 9 36 3.3536904761904776 9 37 2.7832142857142887 9 38 3.0829166666666676 9 39 3.456547619047619 9 40 3.2650595238095246 10 1 -7.7450595238095215 10 2 -6.580178571428575 10 3 -0.5548214285714259 10 4 -3.923809523809525 10 5 -5.225654761904763 10 6 -2.7037499999999977 10 7 -3.7932142857142885 10 8 -3.2281547619047615 10 9 -2.5787499999999994 10 10 -2.569642857142858 10 11 2.70482142857143 10 12 1.3708928571428576 10 13 1.765833333333335 10 14 -4.782142857142857 10 15 2.941011904761904 10 16 3.3429166666666656 10 17 3.3112499999999994 10 18 2.0846428571428586 10 19 2.5336904761904755 10 20 2.3615476190476183 10 21 3.8874999999999993 10 22 3.3120238095238097 10 23 3.138571428571428 10 24 2.8704166666666655 10 25 2.925238095238095 10 26 2.9659523809523822 10 27 0.7070238095238093 10 28 2.870178571428575 10 29 1.3326785714285734 10 30 -2.317619047619049 10 31 0.5146428571428565 10 32 1.331309523809523 10 33 0.5250595238095244 10 34 0.010833333333334139 10 35 0.40547619047619143 10 36 2.3536904761904776 10 37 2.116547619047621 10 38 0.08291666666666764 10 39 -0.543452380952381 10 40 0.9317261904761924 15 1 -8.078392857142854 15 2 -3.2468452380952417 15 3 -3.221488095238092 15 4 -3.257142857142858 15 5 -4.558988095238096 15 6 -6.037083333333332 15 7 -8.459880952380956 15 8 -5.894821428571429 15 9 -3.5787499999999994 15 10 -3.569642857142857 15 11 -3.961845238095238 15 12 -2.6291071428571424 15 13 -4.567499999999999 15 14 -0.7821428571428566 15 15 -6.39232142857143 15 16 -0.9904166666666665 15 17 -2.6887499999999998 15 18 0.4179761904761925 15 19 0.5336904761904755 15 20 -0.30511904761904773 15 21 1.2208333333333332 15 22 3.3120238095238097 15 23 2.138571428571428 15 24 0.8704166666666655 15 25 1.925238095238095 15 26 3.299285714285716 15 27 3.373690476190477 15 28 0.20351190476190695 15 29 3.3326785714285734 15 30 3.349047619047619 15 31 2.1813095238095244 15 32 2.997976190476191 15 33 3.5250595238095244 15 34 3.010833333333334 15 35 3.4054761904761914 15 36 4.020357142857145 15 37 3.7832142857142887 15 38 3.7495833333333355 15 39 4.123214285714287 15 40 3.9317261904761924 17 1 -6.4117261904761875 17 2 3.586488095238092 17 3 2.778511904761908 17 4 -2.757142857142858 17 5 -0.058988095238095895 17 6 1.4629166666666684 17 7 -1.7932142857142885 17 8 0.9385119047619046 17 9 1.2545833333333345 17 10 -1.736309523809524 17 11 -1.128511904761904 17 12 -3.4624404761904763 17 13 -2.567499999999999 17 14 -4.782142857142857 17 15 -1.3923214285714298 17 16 4.67625 17 17 4.144583333333333 17 18 2.0846428571428586 17 19 2.5336904761904755 17 20 0.6948809523809523 17 21 6.720833333333333 17 22 8.645357142857144 17 23 8.971904761904762 17 24 9.20375 17 25 8.758571428571429 17 26 8.465952380952382 17 27 8.540357142857143 17 28 9.03684523809524 17 29 8.49934523809524 17 30 9.015714285714285 17 31 7.84797619047619 17 32 8.164642857142857 17 33 8.69172619047619 17 34 8.1775 17 35 8.572142857142858 17 36 9.687023809523811 17 37 8.949880952380955 17 38 8.916250000000002 17 39 9.789880952380953 17 40 9.098392857142859 18 1 -0.7450595238095197 18 2 -0.5801785714285739 18 3 2.111845238095242 18 4 1.076190476190476 18 5 3.774345238095238 18 6 3.2962500000000006 18 7 2.2067857142857115 18 8 5.1051785714285725 18 9 5.087916666666667 18 10 0.7636904761904759 18 11 11.038154761904764 18 12 7.037559523809524 18 13 7.432500000000001 18 14 10.884523809523811 18 15 1.2743452380952363 18 16 -6.32375 18 17 -5.355416666666667 18 18 -4.915357142857141 18 19 -5.4663095238095245 18 20 -5.305119047619048 18 21 -6.779166666666667 18 22 -6.354642857142856 18 23 -6.528095238095238 18 24 -5.796250000000001 18 25 -6.741428571428571 18 26 -7.034047619047618 18 27 -6.959642857142857 18 28 -6.463154761904759 18 29 -7.0006547619047605 18 30 -6.984285714285715 18 31 -8.15202380952381 18 32 -7.335357142857143 18 33 -6.80827380952381 18 34 -7.3225 18 35 -6.9278571428571425 18 36 -6.3129761904761885 18 37 -6.550119047619045 18 38 -6.583749999999998 18 39 -6.210119047619047 18 40 -6.4016071428571415 19 1 7.5882738095238125 19 2 7.086488095238092 19 3 4.778511904761908 19 4 0.742857142857142 19 5 10.441011904761904 19 6 11.962916666666668 19 7 11.206785714285711 19 8 6.438511904761905 19 9 4.7545833333333345 19 10 -2.236309523809524 19 11 0.3714880952380959 19 12 11.037559523809524 19 13 -5.567499999999999 19 14 -3.7821428571428566 19 15 5.60767857142857 19 16 8.67625 19 17 3.6445833333333333 19 18 13.084642857142859 19 19 12.533690476190475 19 20 9.694880952380952 19 21 12.220833333333333 19 22 12.645357142857144 19 23 11.471904761904762 19 24 5.203749999999999 19 25 11.258571428571429 19 26 10.965952380952382 19 27 9.040357142857143 19 28 4.536845238095241 19 29 -6.54761904760548E-4 19 30 8.015714285714285 19 31 14.84797619047619 19 32 5.664642857142857 19 33 12.19172619047619 19 34 11.6775 19 35 11.072142857142858 19 36 20.68702380952381 19 37 20.449880952380955 19 38 14.416250000000002 19 39 20.789880952380955 19 40 20.59839285714286 23 1 -15.411726190476188 23 2 -8.913511904761908 23 3 -7.221488095238092 23 4 -6.257142857142858 23 5 -7.558988095238096 23 6 -10.037083333333332 23 7 -10.793214285714289 23 8 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-0.2454166666666655 24 10 11.763690476190476 24 11 16.371488095238096 24 12 16.037559523809524 24 13 12.432500000000001 24 14 17.21785714285714 24 15 18.607678571428572 24 16 -6.32375 24 17 -5.355416666666667 24 18 -4.915357142857141 24 19 -5.4663095238095245 24 20 -5.305119047619048 24 21 -6.779166666666667 24 22 -6.354642857142856 24 23 -6.528095238095238 24 24 -5.796250000000001 24 25 -6.741428571428571 24 26 -7.034047619047618 24 27 -6.959642857142857 24 28 -6.463154761904759 24 29 -7.0006547619047605 24 30 -6.984285714285715 24 31 -8.15202380952381 24 32 -7.335357142857143 24 33 -6.80827380952381 24 34 -7.3225 24 35 -6.9278571428571425 24 36 -6.3129761904761885 24 37 -6.550119047619045 24 38 -6.583749999999998 24 39 -6.210119047619047 24 40 -6.4016071428571415 26 1 3.5882738095238125 26 2 9.086488095238092 26 3 7.778511904761908 26 4 0.742857142857142 26 5 2.441011904761904 26 6 7.962916666666668 26 7 8.206785714285711 26 8 9.438511904761905 26 9 5.7545833333333345 26 10 12.763690476190476 26 11 22.371488095238096 26 12 22.037559523809524 26 13 22.4325 26 14 22.21785714285714 26 15 4.60767857142857 26 16 16.67625 26 17 25.644583333333333 26 18 26.08464285714286 26 19 23.533690476190475 26 20 20.694880952380952 26 21 -6.779166666666667 26 22 -6.354642857142856 26 23 -6.528095238095238 26 24 -5.796250000000001 26 25 -6.741428571428571 26 26 -7.034047619047618 26 27 -6.959642857142857 26 28 -6.463154761904759 26 29 -7.0006547619047605 26 30 -6.984285714285715 26 31 -8.15202380952381 26 32 -7.335357142857143 26 33 -6.80827380952381 26 34 -7.3225 26 35 -6.9278571428571425 26 36 -6.3129761904761885 26 37 -6.550119047619045 26 38 -6.583749999999998 26 39 -6.210119047619047 26 40 -6.4016071428571415 27 1 9.588273809523812 27 2 17.086488095238092 27 3 5.778511904761908 27 4 0.742857142857142 27 5 16.441011904761904 27 6 13.962916666666668 27 7 18.20678571428571 27 8 17.438511904761903 27 9 18.754583333333336 27 10 21.763690476190476 27 11 -8.628511904761904 27 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-6.984285714285715 93 31 -8.15202380952381 93 32 -7.335357142857143 93 33 -6.80827380952381 93 34 -7.3225 93 35 -6.9278571428571425 93 36 -6.3129761904761885 93 37 -6.550119047619045 93 38 -6.583749999999998 93 39 -6.210119047619047 93 40 -6.4016071428571415 95 1 -4.4117261904761875 95 2 0.0864880952380922 95 3 1.278511904761908 95 4 -0.757142857142858 95 5 -4.558988095238096 95 6 -1.0370833333333316 95 7 0.20678571428571146 95 8 0.9385119047619046 95 9 1.2545833333333345 95 10 -0.23630952380952408 95 11 -4.628511904761904 95 12 2.0375595238095237 95 13 4.432500000000001 95 14 2.2178571428571434 95 15 2.60767857142857 95 16 -1.8237500000000004 95 17 7.644583333333333 95 18 -4.915357142857141 95 19 -5.4663095238095245 95 20 -5.305119047619048 95 21 -6.779166666666667 95 22 -6.354642857142856 95 23 -6.528095238095238 95 24 -5.796250000000001 95 25 -6.741428571428571 95 26 -7.034047619047618 95 27 -6.959642857142857 95 28 -6.463154761904759 95 29 -7.0006547619047605 95 30 -6.984285714285715 95 31 -8.15202380952381 95 32 -7.335357142857143 95 33 -6.80827380952381 95 34 -7.3225 95 35 -6.9278571428571425 95 36 -6.3129761904761885 95 37 -6.550119047619045 95 38 -6.583749999999998 95 39 -6.210119047619047 95 40 -6.4016071428571415 101 1 7.25494047619048 101 2 8.75315476190476 101 3 4.111845238095242 101 4 3.409523809523808 101 5 10.441011904761904 101 6 12.29625 101 7 13.87345238095238 101 8 13.105178571428572 101 9 13.421250000000002 101 10 10.763690476190476 101 11 9.704821428571428 101 12 14.037559523809524 101 13 12.099166666666669 101 14 10.217857142857143 101 15 7.941011904761902 101 16 16.342916666666667 101 17 11.977916666666665 101 18 13.751309523809526 101 19 14.200357142857143 101 20 15.028214285714284 101 21 9.220833333333333 101 22 5.645357142857144 101 23 12.471904761904762 101 24 8.20375 101 25 11.925238095238097 101 26 10.965952380952382 101 27 9.040357142857143 101 28 9.53684523809524 101 29 11.666011904761907 101 30 11.682380952380953 101 31 11.514642857142858 101 32 4.997976190476189 101 33 11.858392857142858 101 34 11.6775 101 35 13.072142857142858 101 36 1.6870238095238115 101 37 1.7832142857142887 101 38 2.0829166666666676 101 39 1.789880952380953 101 40 1.5983928571428585 104 1 -1.0783928571428554 104 2 -2.2468452380952417 104 3 1.778511904761908 104 4 -0.257142857142858 104 5 5.441011904761904 104 6 -0.03708333333333158 104 7 2.5401190476190436 104 8 3.4385119047619046 104 9 -1.5787499999999994 104 10 -2.569642857142858 104 11 3.371488095238096 104 12 4.7042261904761915 104 13 5.432500000000001 104 14 6.551190476190476 104 15 5.274345238095238 104 16 1.0095833333333335 104 17 -0.3554166666666667 104 18 -2.5820238095238084 104 19 -2.1329761904761915 104 20 -1.3051190476190477 104 21 2.554166666666667 104 22 1.9786904761904776 104 23 2.8052380952380958 104 24 3.5370833333333334 104 25 2.591904761904763 104 26 3.299285714285716 104 27 3.373690476190477 104 28 3.870178571428575 104 29 3.3326785714285734 104 30 3.349047619047619 104 31 2.1813095238095244 104 32 2.664642857142857 104 33 3.5250595238095244 104 34 3.010833333333334 104 35 3.4054761904761914 104 36 4.020357142857145 104 37 3.7832142857142887 104 38 3.7495833333333355 104 39 4.123214285714287 104 40 2.9317261904761924 107 1 -8.011726190476187 107 2 -2.313511904761908 107 3 0.17851190476190837 107 4 -3.057142857142858 107 5 -1.7589880952380952 107 6 -1.6370833333333312 107 7 -3.5932142857142892 107 8 -1.9614880952380958 107 9 -2.645416666666666 107 10 -2.4363095238095234 107 11 3.9714880952380973 107 12 3.4375595238095222 107 13 3.432500000000001 107 14 4.217857142857143 107 15 -0.7923214285714302 107 16 -1.5237499999999997 107 17 -2.3554166666666667 107 18 0.48464285714285893 107 19 -0.6663095238095238 107 20 0.09488095238095262 107 21 -0.17916666666666714 107 22 -0.15464285714285708 107 23 -0.5280952380952382 107 24 0.8037499999999991 107 25 -0.14142857142857146 107 26 -3.234047619047618 107 27 -0.7596428571428575 107 28 -0.06315476190475877 107 29 -0.6006547619047602 107 30 -0.18428571428571416 107 31 -8.15202380952381 107 32 -7.335357142857143 107 33 -6.80827380952381 107 34 -7.3225 107 35 -6.9278571428571425 107 36 -6.3129761904761885 107 37 -6.550119047619045 107 38 -6.583749999999998 107 39 -6.210119047619047 107 40 -6.4016071428571415 108 1 -7.7450595238095215 108 2 -1.5801785714285739 108 3 -4.888154761904759 108 4 -2.590476190476191 108 5 -1.225654761904762 108 6 -5.037083333333332 108 7 -4.7932142857142885 108 8 -3.5614880952380954 108 9 -2.5787499999999994 108 10 -3.569642857142857 108 11 0.20482142857142982 108 12 -2.4624404761904763 108 13 1.265833333333335 108 14 1.0511904761904773 108 15 1.1076785714285702 108 16 -0.6570833333333344 108 17 -1.0220833333333328 108 18 -0.0820238095238075 108 19 0.20035714285714157 108 20 0.36154761904761834 108 21 -2.445833333333333 108 22 -2.1879761904761903 108 23 -4.528095238095238 108 24 -1.1295833333333345 108 25 -3.908095238095238 108 26 -5.200714285714285 108 27 -5.29297619047619 108 28 -5.463154761904759 108 29 -5.5006547619047605 108 30 -5.817619047619048 108 31 -2.8186904761904756 108 32 -2.002023809523809 108 33 -2.3082738095238096 108 34 -2.8225 108 35 -2.4278571428571425 108 36 -6.3129761904761885 108 37 -6.550119047619045 108 38 -6.583749999999998 108 39 -6.210119047619047 108 40 -6.4016071428571415 109 1 -6.611726190476187 109 2 -1.7135119047619085 109 3 -1.8214880952380916 109 4 -3.057142857142858 109 5 -6.758988095238096 109 6 -0.43708333333333194 109 7 -3.393214285714288 109 8 -3.7614880952380947 109 9 -1.4454166666666648 109 10 -3.4363095238095243 109 11 -2.428511904761905 109 12 -2.562440476190476 109 13 -3.3674999999999997 109 14 -3.5821428571428573 109 15 -3.592321428571429 109 16 -2.3237500000000004 109 17 -4.355416666666667 109 18 1.484642857142859 109 19 -0.2663095238095252 109 20 0.8948809523809516 109 21 -1.1791666666666671 109 22 0.44535714285714434 109 23 -0.5280952380952382 109 24 -1.7962500000000006 109 25 -2.541428571428572 109 26 2.3659523809523826 109 27 2.2403571428571425 109 28 3.9368452380952412 109 29 3.1993452380952387 109 30 3.815714285714286 109 31 4.247976190476189 109 32 5.0646428571428554 109 33 5.591726190476189 109 34 5.077499999999999 109 35 5.472142857142856 109 36 -0.7129761904761889 109 37 -0.3501190476190459 109 38 -0.38374999999999915 109 39 -0.010119047619047805 109 40 -0.2016071428571422 111 1 6.5882738095238125 111 2 3.086488095238092 111 3 -1.221488095238092 111 4 0.742857142857142 111 5 10.441011904761904 111 6 15.962916666666668 111 7 15.206785714285711 111 8 6.438511904761905 111 9 10.754583333333334 111 10 0.7636904761904759 111 11 -4.628511904761904 111 12 1.0375595238095237 111 13 2.432500000000001 111 14 -6.782142857142857 111 15 -5.39232142857143 111 16 9.67625 111 17 4.644583333333333 111 18 8.084642857142859 111 19 12.533690476190475 111 20 2.6948809523809523 111 21 2.220833333333333 111 22 -3.3546428571428564 111 23 1.4719047619047618 111 24 5.203749999999999 111 25 11.258571428571429 111 26 11.965952380952382 111 27 17.040357142857143 111 28 17.53684523809524 111 29 9.99934523809524 111 30 14.015714285714285 111 31 22.84797619047619 111 32 13.664642857142857 111 33 20.19172619047619 111 34 23.677500000000002 111 35 24.072142857142858 111 36 24.68702380952381 111 37 24.449880952380955 111 38 24.41625 111 39 20.789880952380955 111 40 24.59839285714286 120 1 10.588273809523812 120 2 -8.913511904761908 120 3 -7.221488095238092 120 4 -6.257142857142858 120 5 -7.558988095238096 120 6 -10.037083333333332 120 7 -10.793214285714289 120 8 -11.561488095238095 120 9 -10.245416666666666 120 10 -7.236309523809524 120 11 -8.628511904761904 120 12 -8.962440476190476 120 13 -8.567499999999999 120 14 -8.782142857142857 120 15 -8.39232142857143 120 16 -6.32375 120 17 -5.355416666666667 120 18 -4.915357142857141 120 19 -5.4663095238095245 120 20 -5.305119047619048 120 21 -6.779166666666667 120 22 -6.354642857142856 120 23 -6.528095238095238 120 24 -5.796250000000001 120 25 -6.741428571428571 120 26 -7.034047619047618 120 27 -6.959642857142857 120 28 -6.463154761904759 120 29 -7.0006547619047605 120 30 -6.984285714285715 120 31 -8.15202380952381 120 32 -7.335357142857143 120 33 -6.80827380952381 120 34 -7.3225 120 35 -6.9278571428571425 120 36 -6.3129761904761885 120 37 -6.550119047619045 120 38 -6.583749999999998 120 39 -6.210119047619047 120 40 -6.4016071428571415 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 595.0 5 10.0 6 26.0 7 24.0 8 16.0 9 22.0 10 30.0 11 60.0 12 32.0 13 9.0 14 19.0 15 20.0 16 6.0 17 10.0 18 6.0 19 7.0 20 26.0 21 24.0 22 22.0 23 31.0 24 28.0 25 16.0 26 26.0 27 14.0 28 6.0 29 4.0 30 0.0 31 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 100.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 98.06807727690892 0.7359705611775529 0.6439742410303588 0.5519779208831647 8 97.06422018348624 1.4678899082568808 0.7339449541284404 0.7339449541284404 9 94.67889908256882 2.2018348623853212 2.2018348623853212 0.9174311926605505 10 40.091743119266056 32.75229357798165 11.284403669724771 15.871559633027523 11 32.477064220183486 24.86238532110092 20.458715596330276 22.201834862385322 12 36.69724770642202 12.935779816513762 23.94495412844037 26.422018348623855 13 24.128440366972477 16.88073394495413 30.36697247706422 28.623853211009177 14 18.440366972477065 20.825688073394495 34.03669724770642 26.69724770642202 15 25.688073394495415 19.541284403669724 31.46788990825688 23.30275229357798 16 26.146788990825687 18.53211009174312 29.44954128440367 25.871559633027523 17 21.743119266055047 21.009174311926607 32.29357798165138 24.954128440366972 18 20.0 19.908256880733948 30.825688073394495 29.266055045871557 19 14.825046040515655 20.534069981583794 26.795580110497237 37.84530386740331 20 20.733944954128443 20.642201834862387 28.807339449541285 29.81651376146789 21 16.238532110091743 19.541284403669724 34.4954128440367 29.724770642201836 22 15.504587155963304 20.18348623853211 34.4954128440367 29.81651376146789 23 18.53211009174312 16.972477064220186 34.12844036697248 30.36697247706422 24 16.14678899082569 20.733944954128443 34.12844036697248 28.990825688073397 25 17.55514705882353 14.79779411764706 28.58455882352941 39.0625 26 14.180478821362799 14.64088397790055 30.018416206261513 41.16022099447514 27 12.65004616805171 14.035087719298245 35.641735918744224 37.67313019390582 28 14.746543778801843 17.419354838709676 31.889400921658982 35.944700460829495 29 16.007359705611776 16.007359705611776 37.16651333946642 30.818767249310024 30 14.522058823529413 14.981617647058822 40.533088235294116 29.963235294117645 31 14.600550964187327 14.049586776859504 38.56749311294766 32.78236914600551 32 18.807339449541285 14.587155963302752 39.633027522935784 26.972477064220186 33 17.79816513761468 10.733944954128441 40.825688073394495 30.642201834862387 34 14.036697247706423 13.119266055045872 47.522935779816514 25.321100917431195 35 12.568807339449542 15.229357798165138 47.706422018348626 24.4954128440367 36 13.486238532110093 19.08256880733945 44.03669724770643 23.394495412844037 37 13.573407202216067 16.62049861495845 48.10710987996307 21.69898430286242 38 14.220183486238533 14.036697247706423 44.77064220183487 26.972477064220186 39 14.220183486238533 12.752293577981652 45.412844036697244 27.614678899082566 40 13.577981651376147 13.394495412844037 45.04587155963303 27.981651376146786 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 1.0 11 1.5 12 2.0 13 2.0 14 2.5 15 3.0 16 8.5 17 14.0 18 14.0 19 16.5 20 19.0 21 22.5 22 26.0 23 26.0 24 28.0 25 30.0 26 31.0 27 32.0 28 32.0 29 30.0 30 28.0 31 27.0 32 26.0 33 26.0 34 30.5 35 35.0 36 37.0 37 39.0 38 39.0 39 46.5 40 54.0 41 68.0 42 82.0 43 82.0 44 76.0 45 70.0 46 65.5 47 61.0 48 61.0 49 66.0 50 71.0 51 66.0 52 61.0 53 61.0 54 62.5 55 64.0 56 64.0 57 64.0 58 64.0 59 54.5 60 45.0 61 55.5 62 66.0 63 66.0 64 58.0 65 50.0 66 45.0 67 40.0 68 40.0 69 44.0 70 48.0 71 34.5 72 21.0 73 21.0 74 16.0 75 11.0 76 10.5 77 10.0 78 10.0 79 10.0 80 10.0 81 7.0 82 4.0 83 4.0 84 2.5 85 1.0 86 1.5 87 2.0 88 2.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.27522935779816515 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.3669724770642202 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.1834862385321101 26 0.3669724770642202 27 0.6422018348623854 28 0.45871559633027525 29 0.27522935779816515 30 0.1834862385321101 31 0.09174311926605505 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.6422018348623854 38 0.0 39 0.0 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 1090.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.1651376146789 #Duplication Level Percentage of deduplicated Percentage of total 1 98.8785046728972 97.06422018348624 2 0.7476635514018692 1.4678899082568808 3 0.09345794392523366 0.27522935779816515 4 0.1869158878504673 0.7339449541284404 5 0.09345794392523366 0.45871559633027525 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TAGCATGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.45871559633027525 No Hit TAGCATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT 4 0.3669724770642202 No Hit TAGCATGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4 0.3669724770642202 No Hit TAGCATGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3 0.27522935779816515 No Hit TAGCATGGGGAGTCGCCTTTTTTTTTTTTTTTTTTTTTTT 2 0.1834862385321101 No Hit TAGCATGGGTCGTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2 0.1834862385321101 No Hit TAGCATGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTCTT 2 0.1834862385321101 No Hit TAGCATGGGGGGGCTGGTGAGATGGCCCAGTGGGTAAGAG 2 0.1834862385321101 No Hit TAGCATGGGGGGCTGGTGCGATGCCCCAGTGGGTAAGAGC 2 0.1834862385321101 No Hit TAGCATGGGAATGGTTCAACGCAGAGCCGACTTTTTTTTT 2 0.1834862385321101 No Hit TAGCATGGGTGGTGCTTTTGTATTGGTATTGTATGATGAG 2 0.1834862385321101 No Hit TAGCATGGGGCGTCGCCTTTTTTTTTTTTTTTTTTTTTTT 2 0.1834862385321101 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGGAG 20 0.0017072165 34.0 5 TGGGGGG 20 0.0017072165 34.0 6 CATGGGA 25 9.5802614E-5 34.0 4 TAGCATG 110 0.0 32.454544 1 AGCATGG 110 0.0 32.454544 2 GCATGGG 110 0.0 30.90909 3 CATGGGG 45 3.3740434E-8 30.222221 4 ATGGGGG 40 7.271574E-7 29.75 5 CATGGGC 25 0.005113337 27.199999 4 >>END_MODULE