FastQCFastQC Report
Fri 17 Jun 2016
SRR1526007_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526007_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1192
Sequences flagged as poor quality0
Sequence length40
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCATGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT131.0906040268456376No Hit
TAGCATGGGACTAATCCTAGCCCTAGCCCTACACAAATAT80.6711409395973155No Hit
TAGCATGGGAAGTTAGTCATTCTCACTTCTCAAAATAACA70.587248322147651No Hit
TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT60.5033557046979865No Hit
TAGCATGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG60.5033557046979865No Hit
TAGCATGGGGGAAGCTTTGGTGGTGGTGTCTTGACACTTG50.41946308724832215No Hit
TAGCATGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT40.33557046979865773No Hit
TAGCATGGGATGCCACAACTAGATACATCAACATGATTTA30.25167785234899326No Hit
TAGCATGGGTGCAGTGCTTAGCTGGGGGGGTTGCTATGGC30.25167785234899326No Hit
TAGCATGGGGGAAGATCATGTCTTCCCCTCTTTCCAAAGA30.25167785234899326No Hit
TAGCATGGGGAGTGCAGTGCTTAGCATGGGTCACAGATCC30.25167785234899326No Hit
TAGCATGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG30.25167785234899326No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC30.25167785234899326No Hit
TAGCATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGC20.16778523489932887No Hit
TAGCATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT20.16778523489932887No Hit
TAGCATGGGGGCATTTTGTCGCTTATCCAGTCTTCTACTC20.16778523489932887No Hit
TAGCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAACG20.16778523489932887No Hit
TAGCATGGGCCGATGACTCTTAACGAAGAAAGGTGGCTGA20.16778523489932887No Hit
TAGCATGGGGAGTTTGACCACAGTATCTAGCTATGGCGGC20.16778523489932887No Hit
TAGCATGGGGGGTGCGGGTGCAGTAGAAGTGGCACTGGCA20.16778523489932887No Hit
TAGCATGGGGCTTAGCATGGGTCACAGATCCTCTTCTGAG20.16778523489932887No Hit
TAGCATGGGCAAGGTGGAAGAACTGGTGGGTGAGAGGCAT20.16778523489932887No Hit
TAGCATGGGGGGCCGGAGAGATGGCTCAGAGGTTAAGAGC20.16778523489932887No Hit
TAGCATGGGGTGGACGTTGAGTGTGTGTGATGGCCGGCTG20.16778523489932887No Hit
TAGCATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.16778523489932887No Hit
TAGCATGGGACGATGACTCTTAACGAAGAAAGGTGGCTGA20.16778523489932887No Hit
TAGCATGGGATGTTACTTTATGCTGCTGGCTCTTGTACCT20.16778523489932887No Hit
TAGCATGGGTCGCAGATCCTCTTCTGAAGGAGATTGGGCG20.16778523489932887No Hit
TAGCATGGGGGTTTGTTTGTCTTTTAATTGTTTTTTTTTT20.16778523489932887No Hit
TAGCATGGGGACATTTCAGACAAGGACTGCATCCCAGAGA20.16778523489932887No Hit
TAGCATGGGATGGTTCTTAAAGGTATTTATTAGAAATTAA20.16778523489932887No Hit
TAGCATGGGGTGTTCTTTATTAATATCCTAACACTCCTCG20.16778523489932887No Hit
TAGCATGGGATTCGTAGGAGTAAACATAACATTCTTCCCT20.16778523489932887No Hit
TAGCATGGGGAGTCCTCTCCTAGGACTATCCCCGGTTCTG20.16778523489932887No Hit
TAGCATGGGGTGTTCTGTGCCTCCTCATCCCACAGTATTT20.16778523489932887No Hit
TAGCATGGGGGAAAAAACCTTTAATAGTGAATAATTAACA20.16778523489932887No Hit
TAGCATGGGATTTTGGATGATTAGAAGCACAGAATGATCA20.16778523489932887No Hit
TAGCATGGGGTCTTGTAAATGTTCATTGCAAAGATGACTA20.16778523489932887No Hit
TAGCATGGGGATACTTTAATATTCTTAAACTCATTGTATT20.16778523489932887No Hit
TAGCATGGGTACCTGGTTGACCCTGCCAGTAGCATATGCT20.16778523489932887No Hit
TAGCATGGGGAAATTTAAGAGTTATAATAAAGCATGATGA20.16778523489932887No Hit
TAGCATAGGAGAAGGCCTGGGCCCAGTGGTGCCTGCAGGG20.16778523489932887No Hit
TAGCATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.16778523489932887No Hit
TAGCATGGGTCACCGATCCTCTTCTGAGATGAGTTTTTGT20.16778523489932887No Hit
TAGCATGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT20.16778523489932887No Hit
TAGCATGGGACTCTTCATGTACCTGAACGAAGTTGCGGGC20.16778523489932887No Hit
TAGCATGGGAGTCTGTTTGTTTGTTTGTTTGTTTGTTTGT20.16778523489932887No Hit
TAGCATGGGATCTTAACATGGAATTCACAATTTACCTCTC20.16778523489932887No Hit
TAGCATGGGGTGTACTTTCTTGCTGAGGGTAACTTAACAG20.16778523489932887No Hit
TAGCATGGGTCACAGATCCTCTTCTAGATGAGTTTTTGTT20.16778523489932887No Hit
TAGCATGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT20.16778523489932887No Hit
TAGCATGGGGTCTGTCAAAGGGATGTAAAATGTACACTTG20.16778523489932887No Hit
TAGCATGGGGAGTGTGGTTCACTTTGGGTAGCTGGGTACT20.16778523489932887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGG352.9377952E-734.05
ATGGGAC200.001787722834.05
TAGCATG1150.034.01
TGGGGGA200.001787722834.06
GGGGGAA200.001787722834.07
GCATGGG1150.034.03
AGCATGG1150.034.02
CATGGGG650.034.04
CATGGGA401.4437319E-834.04
Produced by FastQC (version 0.11.5)