FastQCFastQC Report
Fri 17 Jun 2016
SRR1526006_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526006_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9854
Sequences flagged as poor quality0
Sequence length56
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT1851.8774101887558352No Hit
CCGCTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC910.9234828496042217No Hit
CCGCTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT520.5277044854881267No Hit
CCGCTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC390.395778364116095No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC270.2740004059265273No Hit
CCGCTAGGGGACCCTCCAGGCCCATCTTGGCTCCTGACTCCTGAGTTGTGCCGATA200.20296326364927947No Hit
CCGCTAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT190.19281510046681552No Hit
CCGCTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC180.18266693728435154No Hit
CCGCTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA180.18266693728435154No Hit
CCGCTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.17251877410188757No Hit
CCGCTAGGGATTGACAGTCTTGCATTTTGTTTTGTCTCGTGATGGTGGTGGTGGTA160.1623706109194236No Hit
CCGCTAGGGACTAATCCTAGCCCTAGCCCTACACAAATATCATTATACTATTATAT160.1623706109194236No Hit
CCGCTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT140.14207428455449564No Hit
CCGCTAGGGGTCTCCCCCACCCTCGGCCCTGCTCCTCTTTGTCTTCTCAATGTGGT120.12177795818956769No Hit
CCGCTAGGGATTCGTAGGAGTAAACATAACATTCTTCCCTCAACATTTCCTGGGCC120.12177795818956769No Hit
CCGCTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA110.11162979500710371No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC110.11162979500710371No Hit
CCGCTAGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC110.11162979500710371No Hit
CCGCTAGGGCCTCCTCCTACCTCCACGATTCCACTCCCTCCTGGACCCTGTCACCC100.10148163182463973No Hit
CCGCTAGGGAATCCTGGAGACAAGACCCTGGAGGACCGGTTTTTTGAACATGAGGT100.10148163182463973No Hit
CCGCTAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTA100.10148163182463973No Hit
CCGCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC100.10148163182463973No Hit
CCGCTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC100.10148163182463973No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCAT150.00782286750.57216637
ACCCGAC150.00782286750.57216641
GATTGAC150.00782286750.57216641
GCGCATT150.00782286750.57216638
CGCATTG150.00782286750.57216639
GCATTGA150.00782286750.57216640
TTGTCCA203.7631602E-450.57216337
CCAATCC203.7631602E-450.57216341
TGTCCAA203.7631602E-450.57216338
TGGGCGC203.7631602E-450.57216335
CCCGACT150.00798474650.3128242
CCGACTG150.00798474650.3128243
AGGGGTG309.713458E-749.8020326
AGGGGTC309.713458E-749.8020326
AGGGGGG900.049.8020326
GGAGAGA150.00831596749.80203215
AGGGGCC150.00831596749.8020326
AGGGGAT451.1459633E-1049.8020326
AGGGGAC150.00831596749.8020326
AGGGGAA451.1459633E-1049.8020326
Produced by FastQC (version 0.11.5)