##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526006_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9854 Sequences flagged as poor quality 0 Sequence length 56 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.64268317434544 39.0 37.0 40.0 32.0 40.0 2 36.13020093363101 39.0 36.0 40.0 29.0 40.0 3 35.069007509640755 38.0 35.0 39.0 25.0 40.0 4 34.91008727420337 38.0 35.0 39.0 23.0 40.0 5 35.215749949259184 39.0 35.0 40.0 24.0 40.0 6 33.61568906028009 38.0 33.0 39.0 15.0 40.0 7 35.505987416277655 38.0 35.0 39.0 28.0 40.0 8 35.57600974223666 38.0 35.0 39.0 28.0 40.0 9 35.52567485285164 38.0 35.0 39.0 27.0 40.0 10 34.47462959204384 38.0 34.0 39.0 23.0 40.0 11 33.99868073878628 38.0 33.0 39.0 23.0 40.0 12 33.17414248021108 38.0 31.0 39.0 20.0 40.0 13 32.31814491577025 37.0 29.0 39.0 16.0 40.0 14 33.16602394966511 38.0 31.0 39.0 19.0 40.0 15 31.959711792165617 36.0 29.0 39.0 15.0 40.0 16 32.60249644814289 37.0 30.0 39.0 19.0 40.0 17 31.097320884919828 36.0 26.0 39.0 14.0 40.0 18 30.663994317028617 35.0 25.0 39.0 13.0 40.0 19 31.95575400852446 36.0 28.0 39.0 17.0 40.0 20 32.68743657398011 36.0 30.0 39.0 20.0 40.0 21 33.35589608280901 38.0 31.0 39.0 19.0 40.0 22 32.368784250050744 38.0 29.0 39.0 16.0 40.0 23 33.30008118530546 38.0 31.0 39.0 18.0 40.0 24 32.53298153034301 37.0 30.0 39.0 17.0 40.0 25 33.16825654556525 38.0 31.0 39.0 19.0 40.0 26 33.44611325350112 38.0 32.0 39.0 18.0 40.0 27 33.75705297341181 38.0 33.0 39.0 20.0 40.0 28 33.88806576009742 38.0 33.0 39.0 22.0 40.0 29 33.26405520600771 38.0 32.0 39.0 17.0 40.0 30 33.09640755023341 38.0 32.0 39.0 17.0 40.0 31 33.959813273797444 38.0 33.0 39.0 21.0 40.0 32 33.94722955145119 38.0 33.0 39.0 21.0 40.0 33 33.75877816115283 38.0 33.0 39.0 19.0 40.0 34 33.479805155266895 38.0 33.0 39.0 19.0 40.0 35 33.70286178201746 38.0 33.0 39.0 20.0 40.0 36 33.08615790541912 37.0 32.0 39.0 20.0 40.0 37 32.903288004871115 37.0 32.0 39.0 18.0 40.0 38 32.452912522833365 37.0 31.0 39.0 15.0 40.0 39 31.396488735538867 36.0 30.0 39.0 4.0 40.0 40 28.935559163791353 35.0 23.0 38.0 4.0 40.0 41 27.30099451999188 33.0 18.0 38.0 4.0 40.0 42 28.078140856504973 33.0 22.0 38.0 4.0 40.0 43 28.406129490562208 33.0 23.0 38.0 4.0 40.0 44 27.681855084229756 33.0 21.0 38.0 4.0 40.0 45 28.587984574791964 33.0 24.0 38.0 4.0 40.0 46 26.627663892835397 31.0 19.0 38.0 4.0 40.0 47 25.67779581895677 30.0 15.0 37.0 4.0 39.0 48 26.34970570326771 31.0 18.0 38.0 4.0 39.0 49 25.424599147554293 30.0 14.0 37.0 4.0 39.0 50 26.257966308098233 31.0 18.0 37.0 4.0 39.0 51 26.488228130708343 32.0 17.0 38.0 4.0 40.0 52 26.250761112238685 32.0 17.0 38.0 4.0 40.0 53 26.247209255124822 32.0 17.0 38.0 4.0 40.0 54 26.058554901562818 32.0 14.0 38.0 4.0 39.0 55 25.68814694540288 32.0 4.0 37.0 4.0 39.0 56 24.77694337324944 31.0 4.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 3.767538806966634 1 2 4.390947224220838 1 3 5.645123367460251 1 4 5.510120992727266 1 5 5.080414476225144 1 6 7.643634464618167 1 7 4.952479870324645 1 8 5.202193430505346 1 9 5.232724159868248 1 10 6.1112880281689 1 11 -0.6271307028227504 1 12 -5.4355193283950065 1 13 -16.603204711886125 1 14 -5.034270392649027 1 15 2.576126827393505 1 16 -2.5856012856259873 1 17 -5.168918817355539 1 18 -3.748465675177247 1 19 -0.8696794301156707 1 20 -0.5462314042578562 1 21 6.172670935918582 1 22 7.747691996036057 1 23 6.514634122536982 1 24 7.867170784397672 1 25 5.816252300316815 1 26 6.533098577426305 1 27 5.780864178250507 1 28 6.3614021071200995 1 29 6.575374601369408 1 30 7.5636365714529425 1 31 6.015676373387681 1 32 5.900093456414517 1 33 7.012777055152235 1 34 4.846128981552859 1 35 5.94969482994075 1 36 5.740656382531075 1 37 5.671514870859905 1 38 6.215164345595632 1 39 6.912839613426613 1 40 -12.56024071770488 1 41 -1.4951199052224702 1 42 -2.2987831585397203 1 43 -8.188303874271494 1 44 -15.616124499299241 1 45 -1.73047927658655 1 46 -8.379593445769915 1 47 -12.488541743997747 1 48 -10.658510803196906 1 49 -14.934253730972717 1 50 -7.261175032881653 1 51 -2.9705057533777257 1 52 -2.270269454278356 1 53 -2.4818283538562795 1 54 -2.4582091385848095 1 55 -2.9474971148475895 1 56 2.205519618377995 2 1 3.263372140299964 2 2 3.395113890887508 2 3 4.590956700793583 2 4 4.847620992727265 2 5 4.28874780955848 2 6 6.976967797951502 2 7 4.71914653699131 2 8 4.893860097172016 2 9 4.795224159868248 2 10 6.002954694835566 2 11 5.656202630510585 2 12 2.5103140049383263 2 13 3.092628621447208 2 14 5.899062940684306 2 15 6.230293494060174 2 16 5.939398714374011 2 17 9.210247849311127 2 18 6.372367658156087 2 19 5.842820569884328 2 20 5.599601929075476 2 21 5.097670935918579 2 22 6.231025329369391 2 23 5.610467455870314 2 24 5.817170784397675 2 25 5.287085633650147 2 26 3.3080985774263034 2 27 3.893364178250504 2 28 4.165568773786767 2 29 4.037874601369406 2 30 3.6761365714529397 2 31 2.3990097067210137 2 32 2.962593456414517 2 33 1.9627770551522374 2 34 3.3086289815528573 2 35 0.5205281632740828 2 36 3.228156382531072 2 37 3.254848204193241 2 38 3.8526643455956346 2 39 4.458672946759947 2 40 2.673092615628452 2 41 8.275713428110862 2 42 7.872050174793614 2 43 7.0741961257285055 2 44 -2.028624499299241 2 45 6.852854056746782 2 46 6.5120732208967524 2 47 3.7072915893355862 2 48 4.566489196803094 2 49 1.4282462690272837 2 50 7.026324967118349 2 51 9.129494246622276 2 52 9.754730545721642 2 53 9.739004979477055 2 54 10.466790861415191 2 55 10.085836218485746 2 56 12.618019618377996 3 1 4.100872140299963 3 2 4.657613890887511 3 3 5.511790034126918 3 4 5.910120992727265 3 5 5.747081142891815 3 6 8.176967797951505 3 7 5.21914653699131 3 8 5.202193430505346 3 9 4.499390826534913 3 10 6.1112880281689 3 11 5.572869297177249 3 12 6.364480671604991 3 13 7.263461954780542 3 14 6.765729607350973 3 15 4.976126827393504 3 16 7.947732047707344 3 17 10.09774784931113 3 18 8.38486765815609 3 19 7.796987236550994 3 20 7.587101929075473 3 21 6.839337602585246 3 22 8.94769199603606 3 23 7.247967455870317 3 24 8.667170784397676 3 25 6.6162523003168126 3 26 6.999765244092973 3 27 5.980864178250503 3 28 6.094735440453434 3 29 6.575374601369408 3 30 7.963636571452941 3 31 6.615676373387682 3 32 5.433426789747848 3 33 5.012777055152235 3 34 5.646128981552856 3 35 4.616361496607414 3 36 4.873989715864408 3 37 5.671514870859905 3 38 7.415164345595635 3 39 7.712839613426617 3 40 8.373092615628455 3 41 12.171546761444198 3 42 12.234550174793611 3 43 11.011696125728506 3 44 12.183875500700758 3 45 11.269520723413446 3 46 10.953739887563422 3 47 13.711458256002256 3 48 12.674822530136428 3 49 12.865746269027284 3 50 10.205491633785016 3 51 9.696160913288946 3 52 10.92973054572164 3 53 9.384838312810391 3 54 8.408457528081861 3 55 11.919169551819081 3 56 11.205519618377995 4 1 3.9675388069666297 4 2 4.657613890887511 4 3 5.778456700793583 4 4 5.710120992727262 4 5 5.34708114289181 4 6 6.910301131284832 4 7 4.619146536991309 4 8 3.9355267638386806 4 9 4.166057493201578 4 10 6.177954694835563 4 11 6.23953596384392 4 12 6.631147338271656 4 13 7.8634619547805436 4 14 7.032396274017639 4 15 7.576126827393505 4 16 7.147732047707347 4 17 10.897747849311127 4 18 10.184867658156087 4 19 8.796987236550994 4 20 8.653768595742143 4 21 6.972670935918579 4 22 8.214358662702725 4 23 7.3813007892036495 4 24 8.200504117731008 4 25 5.416252300316817 4 26 7.533098577426305 4 27 5.84753084491717 4 28 5.494735440453432 4 29 6.375374601369405 4 30 6.963636571452941 4 31 4.749009706721015 4 32 4.500093456414518 4 33 6.946110388485572 4 34 5.912795648219522 4 35 2.816361496607417 4 36 5.807323049197738 4 37 6.138181537526574 4 38 6.015164345595636 4 39 5.646172946759947 4 40 -0.5602407177048825 4 41 9.971546761444195 4 42 10.834550174793613 4 43 9.011696125728506 4 44 10.917208834034092 4 45 8.602854056746782 4 46 13.420406554230084 4 47 12.044791589335585 4 48 13.008155863469764 4 49 12.13241293569395 4 50 11.93882496711835 4 51 12.829494246622279 4 52 10.463063879054978 4 53 13.984838312810385 4 54 10.74179086141519 4 55 11.119169551819077 4 56 10.138852951711332 5 1 4.167538806966633 5 2 4.9909472242208395 5 3 6.178456700793582 5 4 6.443454326060596 5 5 5.747081142891815 5 6 8.176967797951505 5 7 4.952479870324645 5 8 5.602193430505352 5 9 5.766057493201579 5 10 6.444621361502236 5 11 6.439535963843916 5 12 7.031147338271662 5 13 7.730128621447211 5 14 8.232396274017642 5 15 8.576126827393505 5 16 9.014398714374014 5 17 10.164414515977793 5 18 10.984867658156084 5 19 9.196987236550992 5 20 8.120435262408812 5 21 8.306004269251915 5 22 9.014358662702723 5 23 8.38130078920365 5 24 8.733837451064339 5 25 7.6829189669834825 5 26 6.933098577426303 5 27 6.114197511583836 5 28 7.161402107120097 5 29 7.6420412680360705 5 30 6.630303238119605 5 31 6.549009706721012 5 32 6.766760123081184 5 33 6.212777055152237 5 34 6.179462314886187 5 35 5.816361496607417 5 36 7.540656382531072 5 37 7.671514870859905 5 38 8.6818310122623 5 39 8.979506280093283 5 40 10.906425948961786 5 41 12.371546761444193 5 42 10.501216841460284 5 43 10.945029459061836 5 44 13.183875500700758 5 45 10.536187390080112 5 46 13.087073220896755 5 47 12.178124922668918 5 48 13.27482253013643 5 49 14.799079602360614 5 50 12.405491633785019 5 51 11.829494246622279 5 52 10.92973054572164 5 53 12.318171646143721 5 54 8.408457528081861 5 55 9.985836218485744 5 56 10.005519618377999 6 1 2.9675388069666297 6 2 4.257613890887505 6 3 5.245123367460252 6 4 5.110120992727268 6 5 5.147081142891814 6 6 5.910301131284832 6 7 4.619146536991309 6 8 4.402193430505349 6 9 4.299390826534918 6 10 6.177954694835563 6 11 6.372869297177253 6 12 7.297814004938328 6 13 5.930128621447206 6 14 5.899062940684306 6 15 7.109460160726844 6 16 7.814398714374011 6 17 9.231081182644463 6 18 10.251534324822757 6 19 7.730320569884331 6 20 6.853768595742146 6 21 7.106004269251912 6 22 7.414358662702721 6 23 7.047967455870314 6 24 8.200504117731008 6 25 5.949585633650148 6 26 6.199765244092969 6 27 5.447530844917171 6 28 4.828068773786768 6 29 6.175374601369409 6 30 5.36363657145294 6 31 3.015676373387681 6 32 2.2334267897478526 6 33 4.212777055152237 6 34 4.0461289815528545 6 35 3.2163614966074157 6 36 6.207323049197736 6 37 6.004848204193241 6 38 5.6151643455956375 6 39 7.379506280093281 6 40 7.106425948961782 6 41 10.63821342811086 6 42 10.434550174793614 6 43 11.345029459061841 6 44 11.983875500700762 6 45 6.536187390080112 6 46 8.887073220896752 6 47 9.711458256002256 6 48 8.008155863469764 6 49 9.799079602360614 6 50 9.005491633785013 6 51 8.496160913288943 6 52 8.92973054572164 6 53 8.051504979477055 6 54 7.208457528081858 6 55 9.185836218485747 6 56 9.672186285044663 7 1 3.700872140299964 7 2 4.857613890887507 7 3 5.711790034126913 7 4 6.176787659393931 7 5 5.813747809558478 7 6 6.8436344646181695 7 7 5.15247987032464 7 8 5.802193430505348 7 9 5.566057493201583 7 10 6.511288028168899 7 11 6.906202630510585 7 12 7.4978140049383235 7 13 8.396795288113875 7 14 7.499062940684308 7 15 7.509460160726842 7 16 8.547732047707346 7 17 10.631081182644461 7 18 11.118200991489417 7 19 7.530320569884328 7 20 7.987101929075479 7 21 6.839337602585246 7 22 8.147691996036055 7 23 7.981300789203644 7 24 7.4005041177310105 7 25 6.816252300316815 7 26 5.999765244092973 7 27 6.714197511583837 7 28 4.694735440453435 7 29 6.108707934702739 7 30 6.36363657145294 7 31 6.149009706721014 7 32 4.566760123081181 7 33 5.212777055152237 7 34 5.44612898155286 7 35 4.549694829940751 7 36 5.540656382531072 7 37 5.804848204193238 7 38 6.348497678928965 7 39 7.379506280093281 7 40 5.173092615628452 7 41 9.838213428110862 7 42 9.76788350812695 7 43 9.011696125728506 7 44 10.450542167367423 7 45 9.669520723413452 7 46 9.887073220896752 7 47 10.644791589335586 7 48 10.674822530136428 7 49 10.932412935693954 7 50 10.738824967118347 7 51 9.762827579955609 7 52 10.196397212388312 7 53 10.251504979477058 7 54 9.675124194748527 7 55 10.585836218485746 7 56 10.405519618377998 8 1 3.950872140299964 8 2 4.707613890887508 8 3 5.278456700793583 8 4 5.826787659393929 8 5 5.247081142891815 8 6 7.143634464618167 8 7 5.552479870324646 8 8 5.518860097172016 8 9 5.649390826534912 8 10 6.29462136150223 8 11 7.48953596384392 8 12 7.947814004938326 8 13 8.863461954780544 8 14 7.482396274017642 8 15 8.959460160726838 8 16 9.397732047707347 8 17 10.064414515977791 8 18 10.101534324822751 8 19 9.446987236550992 8 20 8.537101929075476 8 21 7.306004269251915 8 22 10.064358662702727 8 23 8.13130078920365 8 24 8.65050411773101 8 25 8.016252300316811 8 26 6.933098577426303 8 27 7.2475308449171685 8 28 6.561402107120102 8 29 7.1420412680360705 8 30 8.696969904786275 8 31 7.315676373387678 8 32 7.233426789747853 8 33 6.046110388485573 8 34 5.246128981552857 8 35 6.499694829940747 8 36 7.540656382531072 8 37 6.421514870859905 8 38 8.081831012262299 8 39 9.312839613426611 8 40 7.923092615628452 8 41 11.421546761444198 8 42 11.434550174793614 8 43 10.92836279239517 8 44 12.03387550070076 8 45 10.769520723413446 8 46 13.387073220896752 8 47 15.894791589335586 8 48 14.691489196803094 8 49 14.865746269027284 8 50 14.838824967118349 8 51 13.962827579955611 8 52 13.296397212388307 8 53 12.634838312810391 8 54 14.425124194748527 8 55 14.419169551819081 8 56 14.388852951711332 9 1 1.759695669711732 9 2 2.751731537946334 9 3 3.7196331713818154 9 4 5.145415110374323 9 5 3.7666889860290667 9 6 5.271085445010328 9 7 2.8662053605207234 9 8 2.8570953912896613 9 9 3.4680182775153057 9 10 3.495601753659095 9 11 4.141496748157643 9 12 4.168402240232442 9 13 4.588952150858976 9 14 4.60494529362548 9 15 6.219264082295471 9 16 4.108516361432837 9 17 7.54480667284054 9 18 6.302514716979616 9 19 3.47149704047256 9 20 3.551807811428418 9 21 3.325612112389166 9 22 5.186907682310565 9 23 2.7538498088114878 9 24 4.067170784397675 9 25 3.231938574826618 9 26 2.9330985774263034 9 27 2.933805354721091 9 28 -0.06604887327205589 9 29 3.3871393072517613 9 30 2.5989306890999977 9 31 2.3843038243680716 9 32 0.4295052211204009 9 33 1.4480711727992954 9 34 -0.047988665505965145 9 35 1.9065575750387858 9 36 1.481832853119304 9 37 2.298965851252067 9 38 1.5328114044191636 9 39 2.410878829112889 9 40 -0.003377972606841695 9 41 6.308801663404978 9 42 6.787491351264201 9 43 4.7764020080814475 9 44 4.313287265406643 9 45 1.7793246449820757 9 46 4.8870732208967524 9 47 5.82126217757088 9 48 5.706195079156036 9 49 6.571628621968461 9 50 3.3976484965301132 9 51 2.805964834857569 9 52 4.83561289866282 9 53 1.9338579206535265 9 54 4.812379096709307 9 55 5.0564244537798615 9 56 4.040813736025054 10 1 2.7842054736332997 10 2 4.457613890887508 10 3 5.195123367460248 10 4 4.743454326060601 10 5 4.91374780955848 10 6 7.810301131284838 10 7 4.885813203657975 10 8 5.102193430505352 10 9 4.566057493201583 10 10 4.54462136150223 10 11 6.156202630510585 10 12 7.364480671604991 10 13 6.446795288113872 10 14 6.232396274017642 10 15 7.6261268273935094 10 16 6.814398714374011 10 17 10.647747849311127 10 18 10.684867658156087 10 19 8.946987236550992 10 20 7.370435262408812 10 21 7.222670935918579 10 22 7.897691996036055 10 23 7.047967455870314 10 24 7.9005041177310105 10 25 7.266252300316811 10 26 7.266431910759639 10 27 6.330864178250504 10 28 7.144735440453431 10 29 7.308707934702742 10 30 7.530303238119604 10 31 6.149009706721014 10 32 5.983426789747853 10 33 6.046110388485573 10 34 6.412795648219522 10 35 5.999694829940747 10 36 7.457323049197736 10 37 6.921514870859905 10 38 6.915164345595635 10 39 8.646172946759947 10 40 11.589759282295116 10 41 12.838213428110862 10 42 10.184550174793614 10 43 11.011696125728506 10 44 10.617208834034095 10 45 9.602854056746782 10 46 13.887073220896752 10 47 14.31145825600225 10 48 12.608155863469758 10 49 13.69907960236062 10 50 14.172158300451684 10 51 12.712827579955611 10 52 13.546397212388307 10 53 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-13.446117501573505 116 48 -2.876692621378723 116 49 -5.58879918551817 116 50 -1.7520841237907412 116 51 2.7204033375313657 116 52 1.7660941820852791 116 53 2.5060504340225087 116 54 3.069063588687918 116 55 3.1312907639402923 116 56 6.441883254741633 117 1 2.575872140299964 117 2 3.207613890887508 117 3 2.4034567007935834 117 4 4.660120992727265 117 5 3.2887478095584797 117 6 6.101967797951502 117 7 4.09414653699131 117 8 4.143860097172016 117 9 4.107724159868248 117 10 4.627954694835566 117 11 3.2812026305105846 117 12 -4.302185995061674 117 13 3.780128621447208 117 14 0.5240629406843063 117 15 -3.3322065059398263 117 16 -10.435601285625989 117 17 1.022747849311127 117 18 1.0598676581560866 117 19 0.7803205698843279 117 20 0.6621019290754759 117 21 4.847670935918579 117 22 6.606025329369391 117 23 6.172967455870314 117 24 7.442170784397675 117 25 5.849585633650147 117 26 4.433098577426303 117 27 5.705864178250504 117 28 4.228068773786767 117 29 6.475374601369406 117 30 6.36363657145294 117 31 6.024009706721014 117 32 5.400093456414517 117 33 5.212777055152237 117 34 4.246128981552857 117 35 4.958028163274083 117 36 5.790656382531072 117 37 6.879848204193241 117 38 5.915164345595635 117 39 7.521172946759947 117 40 9.548092615628452 117 41 9.088213428110862 117 42 8.059550174793614 117 43 2.1366961257285055 117 44 -2.341124499299241 117 45 8.602854056746782 117 46 0.38707322089675245 117 47 1.0197915893355862 117 48 0.06648919680309362 117 49 -3.1342537309727163 117 50 4.088824967118349 117 51 9.629494246622276 117 52 10.004730545721642 117 53 9.301504979477055 117 54 9.591790861415191 117 55 9.585836218485746 117 56 14.055519618377996 118 1 3.450872140299964 118 2 4.207613890887508 118 3 5.528456700793583 118 4 5.285120992727265 118 5 4.78874780955848 118 6 7.226967797951502 118 7 4.46914653699131 118 8 4.518860097172016 118 9 4.232724159868248 118 10 3.002954694835566 118 11 0.6562026305105846 118 12 -4.427185995061674 118 13 -5.344871378552792 118 14 -6.350937059315694 118 15 -8.582206505939826 118 16 -10.060601285625989 118 17 -0.47725215068887294 118 18 -12.690132341843913 118 19 -1.4696794301156721 118 20 -0.7128980709245241 118 21 5.097670935918579 118 22 6.606025329369391 118 23 5.297967455870314 118 24 6.317170784397675 118 25 4.349585633650147 118 26 6.433098577426303 118 27 5.205864178250504 118 28 6.478068773786767 118 29 6.850374601369406 118 30 6.86363657145294 118 31 4.649009706721014 118 32 3.775093456414517 118 33 4.337777055152237 118 34 5.246128981552857 118 35 4.708028163274083 118 36 4.665656382531072 118 37 5.004848204193241 118 38 4.790164345595635 118 39 7.771172946759947 118 40 6.673092615628452 118 41 2.838213428110862 118 42 2.934550174793614 118 43 -5.1133038742714945 118 44 0.15887550070075918 118 45 1.1028540567467822 118 46 -4.7379267791032476 118 47 -9.480208410664414 118 48 -7.183510803196906 118 49 -7.884253730972716 118 50 -4.286175032881651 118 51 -0.9955057533777243 118 52 -0.9952694542783576 118 53 -3.0734950205229445 118 54 -2.408209138584809 118 55 -1.6641637815142545 118 56 1.6805196183779962 119 1 2.700872140299964 119 2 2.6798361131097295 119 3 4.556234478571362 119 4 2.576787659393929 119 5 3.1915255873362582 119 6 6.310301131284838 119 7 3.7747020925468675 119 8 4.046637874949795 119 9 4.010501937646026 119 10 5.155732472613344 119 11 1.9062026305105846 119 12 -7.7466304395061165 119 13 -4.13653804521946 119 14 -5.878714837093472 119 15 1.7094601607268416 119 16 -13.630045730070432 119 17 0.7866367382000163 119 18 -5.1484656751772455 119 19 -3.8030127634490043 119 20 -0.8795647375911919 119 21 3.528226491474136 119 22 6.481025329369391 119 23 4.825745233648092 119 24 5.067170784397675 119 25 5.349585633650147 119 26 7.044209688537418 119 27 5.46975306713939 119 28 5.116957662675652 119 29 6.975374601369406 119 30 6.808081015897383 119 31 3.5934541511654565 119 32 5.788982345303403 119 33 5.212777055152237 119 34 5.246128981552857 119 35 5.083028163274083 119 36 5.985100826975515 119 37 6.227070426415462 119 38 7.30405323448452 119 39 4.9795062800932826 119 40 10.284203726739566 119 41 6.393768983666419 119 42 4.767883508126946 119 43 -3.8771927631603837 119 44 -1.771680054854798 119 45 5.26952072341345 119 46 0.9981843320078632 119 47 -3.4663195217755245 119 48 -2.0585108031969064 119 49 -4.023142619861606 119 50 2.227713856007238 119 51 4.740605357733386 119 52 7.129730545721642 119 53 2.9403938683659447 119 54 2.6751241947485234 119 55 1.6969473295968562 119 56 10.249964062822439 120 1 2.8119832514110783 120 2 3.5687250019986223 120 3 4.445123367460248 120 4 4.910120992727265 120 5 4.524858920669594 120 6 6.643634464618167 120 7 3.8858132036579747 120 8 3.2688600971720163 120 9 3.5660574932015834 120 10 3.2668435837244516 120 11 -0.7604640361560833 120 12 -2.635519328395006 120 13 -7.025426934108349 120 14 -5.2120481704268045 120 15 -3.623873172606494 120 16 -9.518934618959321 120 17 0.897747849311127 120 18 1.5182009914894188 120 19 0.3080983476621064 120 20 0.12043526240880809 120 21 5.639337602585243 120 22 8.147691996036055 120 23 6.492411900314757 120 24 6.622726339953232 120 25 6.016252300316811 120 26 4.599765244092968 120 27 5.803086400472726 120 28 1.6725132182312095 120 29 5.419819045813849 120 30 6.474747682564054 120 31 3.1490097067210137 120 32 4.566760123081181 120 33 3.434999277374459 120 34 4.468351203775079 120 35 4.9719170521629685 120 36 4.985100826975515 120 37 5.115959315304355 120 38 6.415164345595635 120 39 5.535061835648833 120 40 8.95087039340623 120 41 -6.050675460778027 120 42 -3.009894269650829 120 43 -1.9883038742714945 120 44 -15.660568943743685 120 45 -3.1749237210309964 120 46 -9.779593445769914 120 47 -8.355208410664414 120 48 -7.3918441365302385 120 49 -15.356475953194938 120 50 -6.661175032881651 120 51 -1.481616864488835 120 52 -1.3147138987228004 120 53 -1.170717242745166 120 54 -1.8804313608070302 120 55 -1.191941559292033 120 56 2.1388529517113284 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 294.0 5 7.0 6 23.0 7 22.0 8 16.0 9 16.0 10 11.0 11 17.0 12 19.0 13 20.0 14 17.0 15 27.0 16 35.0 17 53.0 18 66.0 19 99.0 20 125.0 21 160.0 22 200.0 23 206.0 24 236.0 25 320.0 26 307.0 27 269.0 28 289.0 29 309.0 30 397.0 31 395.0 32 482.0 33 597.0 34 615.0 35 670.0 36 766.0 37 843.0 38 929.0 39 994.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 0.0 100.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 100.0 0.0 6 0.0 100.0 0.0 0.0 7 99.26933225086259 0.5885934645829105 0.09133346864217577 0.05074081591231987 8 99.55348081997158 0.30444489547391923 0.11162979500710371 0.03044448954739192 9 99.13740612949056 0.25370407956159935 0.4160746904810229 0.19281510046681552 10 49.238887761315205 27.379744266287805 16.764765577430484 6.616602394966511 11 23.320478993302213 22.457885122792774 26.45626141668358 27.76537446722143 12 25.1268520397808 26.466409579866045 25.319667140247613 23.08707124010554 13 20.215141059468237 17.454840673838035 25.41100060888979 36.919017657803934 14 15.8311345646438 38.43109397199107 23.87862796833773 21.8591434950274 15 29.222766217870255 26.070991432068542 23.929008567931458 20.77723378212974 16 22.004467912266453 40.36352558895207 20.664094232331436 16.967912266450043 17 21.601867830677087 26.88051974418841 34.7680438534159 16.749568571718605 18 18.824842703470672 26.060483052567484 23.4929977674041 31.62167647655774 19 18.066416167360615 29.145932771402457 21.74266273992079 31.044988321316136 20 20.554765291607396 27.656980288559236 34.60678723836619 17.18146718146718 21 17.588551710139043 28.0320714503197 24.733583680097432 29.645793159443823 22 15.354170895067993 29.125228333671604 38.248427034706715 17.272173736553682 23 16.196468439212502 30.231378120560176 36.06657195047696 17.505581489750355 24 15.151207631418714 32.920641363913134 23.066774913740613 28.861376090927543 25 15.792144524510302 35.21770019283467 32.77174464630062 16.21841063635441 26 31.06529907586067 34.12206763481263 19.9045394536407 14.908093835685996 27 16.190959878110718 44.59116302691722 19.959370238699847 19.25850685627222 28 24.406332453825858 38.06576009742237 19.707732900345036 17.82017454840674 29 24.44828638258924 39.00132207871454 19.475236448693174 17.07515509000305 30 23.002740838493555 31.976449091462793 29.854837072378437 15.165972997665211 31 27.24781814491577 35.70123807590826 21.818550842297544 15.232392936878425 32 24.93147903766115 39.112780428382905 21.469901532839305 14.485839001116638 33 27.954476171120824 36.10405446600955 21.085255563459 14.856213799410629 34 17.789976618887874 30.94439361593982 35.69177594795161 15.573853817220698 35 15.98048185422385 34.00426959438853 35.86459286367795 14.150655687709667 36 20.357396690019293 33.71915930551325 28.337902325109148 17.58554167935831 37 21.894694043504778 33.62472047164058 28.349257979264074 16.13132750559057 38 21.59056239194549 34.557103630631545 27.916200549171162 15.936133428251805 39 25.431822800243854 32.8490144279618 21.17455801666328 20.544604755131072 40 18.82028215088939 25.209568595379267 26.313637292987117 29.656511960744226 41 16.39680262348842 24.369747899159663 23.10924369747899 36.12420577987292 42 14.92415758933116 39.65183752417795 21.816145780311512 23.607859106179376 43 14.45721843524265 35.649608301963575 25.048326381117104 24.84484688167667 44 15.20831211164307 38.17866965468066 26.64765203218906 19.965366201487218 45 21.230925737538147 34.0793489318413 27.761953204476093 16.927772126144454 46 14.6535056477053 39.04548692378142 23.313320443675586 22.98768698483769 47 13.188494492044065 34.49612403100775 20.31823745410037 31.997144022847817 48 22.74573517465475 34.02721364744111 22.918359057676685 20.308692120227455 49 15.318889227952395 35.79493439121148 21.025328043942633 27.8608483368935 50 15.792682926829269 39.25813008130081 22.032520325203254 22.916666666666664 51 15.368163764130765 38.21163051227213 22.120378857317448 24.299826866279663 52 14.84947111472742 45.41293734743694 21.99959316517494 17.737998372660698 53 15.005086469989829 45.27975584944049 23.87589013224822 15.839267548321464 54 15.669515669515668 34.99185999185999 29.14122914122914 20.1973951973952 55 14.919601058416445 33.23834724201099 27.73254630571952 24.10950539385304 56 15.778229908443539 34.4354018311292 29.5116988809766 20.27466937945066 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 1.0 12 2.5 13 46.5 14 89.0 15 150.5 16 212.0 17 230.0 18 248.0 19 222.0 20 196.0 21 177.5 22 152.5 23 146.0 24 143.5 25 141.0 26 129.5 27 118.0 28 125.0 29 149.5 30 167.0 31 169.0 32 171.0 33 179.5 34 188.0 35 196.0 36 204.0 37 235.0 38 263.0 39 260.0 40 267.0 41 274.0 42 285.0 43 296.0 44 320.5 45 345.0 46 404.0 47 639.5 48 816.0 49 824.0 50 832.0 51 701.5 52 571.0 53 511.5 54 459.0 55 466.0 56 451.0 57 436.0 58 413.5 59 391.0 60 380.5 61 370.0 62 377.0 63 364.5 64 345.0 65 296.0 66 247.0 67 208.0 68 169.0 69 138.5 70 108.0 71 95.5 72 62.0 73 41.0 74 35.5 75 30.0 76 22.5 77 15.0 78 12.5 79 6.0 80 2.0 81 2.5 82 3.0 83 2.5 84 2.0 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5074081591231987 16 0.06088897909478384 17 0.03044448954739192 18 0.0 19 0.07103714227724782 20 0.12177795818956769 21 0.010148163182463974 22 0.0 23 0.0 24 0.0 25 0.010148163182463974 26 0.07103714227724782 27 0.09133346864217577 28 0.0 29 0.21311142683174347 30 0.03044448954739192 31 0.0 32 0.03044448954739192 33 0.13192612137203166 34 0.17251877410188757 35 0.17251877410188757 36 0.05074081591231987 37 0.1623706109194236 38 0.21311142683174347 39 0.12177795818956769 40 0.7306677491374062 41 0.9742236655165415 42 0.3145930586563832 43 0.25370407956159935 44 0.375482037751167 45 0.24355591637913537 46 0.2740004059265273 47 0.5074081591231987 48 0.06088897909478384 49 0.2334077531966714 50 0.14207428455449564 51 0.35518571138623906 52 0.22325959001420742 53 0.24355591637913537 54 0.2638522427440633 55 0.2841485691089913 56 0.24355591637913537 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 9854.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.8199715851431 #Duplication Level Percentage of deduplicated Percentage of total 1 89.72877950778503 72.51877410188756 2 6.956303365143144 11.244164806170083 3 1.5946760421898545 3.866450172518774 4 0.6278252134605725 2.029632636492795 5 0.389251632345555 1.5729652932819158 6 0.22601707684580613 1.0960016237061092 7 0.07533902561526871 0.42622285366348694 8 0.07533902561526871 0.48711183275827075 9 0.03766951280763436 0.2740004059265273 >10 0.25113008538422904 3.156078749746296 >50 0.025113008538422906 1.4511873350923483 >100 0.012556504269211453 1.8774101887558352 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCGCTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT 185 1.8774101887558352 No Hit CCGCTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC 91 0.9234828496042217 No Hit CCGCTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT 52 0.5277044854881267 No Hit CCGCTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC 39 0.395778364116095 No Hit CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 27 0.2740004059265273 No Hit CCGCTAGGGGACCCTCCAGGCCCATCTTGGCTCCTGACTCCTGAGTTGTGCCGATA 20 0.20296326364927947 No Hit CCGCTAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT 19 0.19281510046681552 No Hit CCGCTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC 18 0.18266693728435154 No Hit CCGCTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA 18 0.18266693728435154 No Hit CCGCTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 17 0.17251877410188757 No Hit CCGCTAGGGATTGACAGTCTTGCATTTTGTTTTGTCTCGTGATGGTGGTGGTGGTA 16 0.1623706109194236 No Hit CCGCTAGGGACTAATCCTAGCCCTAGCCCTACACAAATATCATTATACTATTATAT 16 0.1623706109194236 No Hit CCGCTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT 14 0.14207428455449564 No Hit CCGCTAGGGGTCTCCCCCACCCTCGGCCCTGCTCCTCTTTGTCTTCTCAATGTGGT 12 0.12177795818956769 No Hit CCGCTAGGGATTCGTAGGAGTAAACATAACATTCTTCCCTCAACATTTCCTGGGCC 12 0.12177795818956769 No Hit CCGCTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA 11 0.11162979500710371 No Hit CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 11 0.11162979500710371 No Hit CCGCTAGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC 11 0.11162979500710371 No Hit CCGCTAGGGCCTCCTCCTACCTCCACGATTCCACTCCCTCCTGGACCCTGTCACCC 10 0.10148163182463973 No Hit CCGCTAGGGAATCCTGGAGACAAGACCCTGGAGGACCGGTTTTTTGAACATGAGGT 10 0.10148163182463973 No Hit CCGCTAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTA 10 0.10148163182463973 No Hit CCGCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 10 0.10148163182463973 No Hit CCGCTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC 10 0.10148163182463973 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.010148163182463974 0.0 0.0 0.0 0.0 7 0.010148163182463974 0.0 0.0 0.0 0.0 8 0.010148163182463974 0.0 0.0 0.0 0.0 9 0.010148163182463974 0.0 0.0 0.0 0.0 10 0.010148163182463974 0.0 0.0 0.0 0.0 11 0.02029632636492795 0.0 0.0 0.0 0.0 12 0.03044448954739192 0.0 0.0 0.0 0.0 13 0.03044448954739192 0.0 0.0 0.0 0.0 14 0.06088897909478384 0.0 0.0 0.0 0.0 15 0.06088897909478384 0.0 0.0 0.0 0.0 16 0.06088897909478384 0.0 0.0 0.0 0.0 17 0.06088897909478384 0.0 0.0 0.0 0.0 18 0.06088897909478384 0.0 0.0 0.0 0.0 19 0.06088897909478384 0.0 0.0 0.0 0.0 20 0.06088897909478384 0.0 0.0 0.0 0.0 21 0.06088897909478384 0.0 0.0 0.0 0.0 22 0.06088897909478384 0.0 0.0 0.0 0.0 23 0.06088897909478384 0.0 0.0 0.0 0.0 24 0.06088897909478384 0.0 0.0 0.0 0.0 25 0.06088897909478384 0.0 0.0 0.0 0.0 26 0.07103714227724782 0.0 0.0 0.0 0.0 27 0.10148163182463973 0.0 0.0 0.0 0.0 28 0.12177795818956769 0.0 0.0 0.0 0.0 29 0.12177795818956769 0.0 0.0 0.0 0.0 30 0.12177795818956769 0.0 0.0 0.0 0.0 31 0.12177795818956769 0.0 0.0 0.0 0.0 32 0.14207428455449564 0.0 0.0 0.0 0.0 33 0.14207428455449564 0.0 0.0 0.0 0.0 34 0.14207428455449564 0.0 0.0 0.0 0.0 35 0.15222244773695962 0.0 0.0 0.0 0.0 36 0.15222244773695962 0.0 0.0 0.0 0.0 37 0.15222244773695962 0.0 0.0 0.0 0.0 38 0.15222244773695962 0.0 0.0 0.0 0.0 39 0.15222244773695962 0.0 0.0 0.0 0.0 40 0.15222244773695962 0.0 0.0 0.0 0.0 41 0.15222244773695962 0.0 0.0 0.010148163182463974 0.0 42 0.15222244773695962 0.0 0.0 0.010148163182463974 0.0 43 0.1623706109194236 0.0 0.0 0.010148163182463974 0.0 44 0.18266693728435154 0.0 0.0 0.010148163182463974 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCGCAT 15 0.007822867 50.572166 37 ACCCGAC 15 0.007822867 50.572166 41 GATTGAC 15 0.007822867 50.572166 41 GCGCATT 15 0.007822867 50.572166 38 CGCATTG 15 0.007822867 50.572166 39 GCATTGA 15 0.007822867 50.572166 40 TTGTCCA 20 3.7631602E-4 50.572163 37 CCAATCC 20 3.7631602E-4 50.572163 41 TGTCCAA 20 3.7631602E-4 50.572163 38 TGGGCGC 20 3.7631602E-4 50.572163 35 CCCGACT 15 0.007984746 50.31282 42 CCGACTG 15 0.007984746 50.31282 43 AGGGGTG 30 9.713458E-7 49.802032 6 AGGGGTC 30 9.713458E-7 49.802032 6 AGGGGGG 90 0.0 49.802032 6 GGAGAGA 15 0.008315967 49.802032 15 AGGGGCC 15 0.008315967 49.802032 6 AGGGGAT 45 1.1459633E-10 49.802032 6 AGGGGAC 15 0.008315967 49.802032 6 AGGGGAA 45 1.1459633E-10 49.802032 6 >>END_MODULE