FastQCFastQC Report
Fri 17 Jun 2016
SRR1526005_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526005_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1335
Sequences flagged as poor quality0
Sequence length40
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCTAGGGTCCAGAGTTCTTTGTCATTACCTTTATGTTA302.247191011235955No Hit
CCGCTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT90.6741573033707865No Hit
CCGCTAGGGATGAGATGGACTTAAAGGAATTGACGGGTAC80.599250936329588No Hit
CCGCTAGGGTCCAGAGTTCTTTGTCATTACCTTTCTGTTA70.5243445692883896No Hit
CCGCTAGGGATGAGATGGCCTTAAAGGAATTGACGGGTAC40.299625468164794No Hit
CCGCTAGGGGGTTTTTTTTACCCCCCCTTTGGTTTTGGTT40.299625468164794No Hit
CCGCTAGGGATGAGATGGACTTAAATGAATTGACGGGTAC40.299625468164794No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG40.299625468164794No Hit
CCGCTAGGGCATGGTAAACGCATCGATTTTTTTTTTTTTT30.22471910112359553No Hit
CCGCTAGGGACATTTGAAGAATATTCTTGGAATAATCTTG30.22471910112359553No Hit
CCGCTAGGGATGAGATGGACTCAAAGGAATTGACGGGTAC30.22471910112359553No Hit
CCGCTAGGGGAGCAGACTTTTCATTCCTCTGGTTCTGTGG30.22471910112359553No Hit
CCGCTAGGGATGAGATGGCCTTAAATGAATTGACGGGTAC30.22471910112359553No Hit
CCGCTAGGGAAGTGTCACGCAGAGTCGACTTTTTTTTTTT20.149812734082397No Hit
CCGCTAGGGGCCGAATAGCCTCTCCACCCCCGCGGCCCGC20.149812734082397No Hit
CCGCTAGGGGGGCAGTGGTGGCGCACGCCTTTAATCCCAG20.149812734082397No Hit
CCGCTAGGGTGGTCACAGCGTCGACTTTTTTTTTTTTTTT20.149812734082397No Hit
CCGCTAGGGTCCAGAGTTCTTTGTCCTTACCTTTATGTTA20.149812734082397No Hit
CCGCTAGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.149812734082397No Hit
CCGCTAGGGGGGCTGGAGCGATGGCTCAGCAGTTAAGAGC20.149812734082397No Hit
CCGCTAGGGGAGTTTCTGTGTGATGTGTTCTGCGTGACGC20.149812734082397No Hit
CCGCTAGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTC20.149812734082397No Hit
CCGCTAGGGCTGAGATGGACTCAAAGGAATTGACGGGTAC20.149812734082397No Hit
CCGCTAGGGATGAGATGGACTTACAGGAATTGACGGGTAC20.149812734082397No Hit
CCGCTAGGGGAGCTCTGTCTTCTTCAAGATCCCTGCACTG20.149812734082397No Hit
CCGCTAGGGGATTCGCTAAGGGATCTTGCAGCTATGGATG20.149812734082397No Hit
CCGCTAGGGGACATCTTGTTTCTTTGTGTTGCCCTTTGCT20.149812734082397No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTACGCGC20.149812734082397No Hit
CCGCTAGGGAAGAGTGGTTCAACGCAGAGTCGACTTTTTT20.149812734082397No Hit
CCGCTAGGGTCAACGCAGCCGATTTTTTTTTTTTTTTTTT20.149812734082397No Hit
CCGCTAGGGGACCCGCGGCAGCATGAAGAAGGTTTTCCCT20.149812734082397No Hit
CCGCTAGGGATGAGATGGACTCACAGGAATTGACGGGTAC20.149812734082397No Hit
CCGCTATGGCCTATAATCCTCTCACTGTAGCCTTATCCCA20.149812734082397No Hit
CCGCTAGGGGCTGGTGAGATGGCTCAGTGGGTAAGCGCCC20.149812734082397No Hit
CCGCTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT20.149812734082397No Hit
CCGCTAGGGATGGTATCACCGCAGATCGACTTTTTTTTTT20.149812734082397No Hit
CCGCTAGGGTCCAGAGTTCTTTGTCATTCCCTTTCTGTTA20.149812734082397No Hit
CCGCTAGGGGGGAATTCCTTCGATTCTGTTTTGCTCCCCG20.149812734082397No Hit
CCGCTAGGGGACCCGCGGCAGCATGACGACGGTTTTCCCT20.149812734082397No Hit
CCGCTAGGGATGAGATGGCCTCAAATGAATTGACGGGTAC20.149812734082397No Hit
CCGCTAGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT20.149812734082397No Hit
CCGCTAGGGATGAGATGGACTCAAATGAATTGACGGGTAC20.149812734082397No Hit
CCGCTAGGGGGGCTGGAGCGATGGCTCAGCGGTTAAGAGC20.149812734082397No Hit
CCGCTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT20.149812734082397No Hit
CCGCTAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAGAGC20.149812734082397No Hit
CCGCTAGGGTACCTGGTTGATCCTGCCCGTAGCCTATGCT20.149812734082397No Hit
CCGCTAGGGGAGCAGCCTTTTCATTCCTCTGGTTCTGTGG20.149812734082397No Hit
CCGCTAGGGGCGGGGATGCGATGGACTTAAATGAATTGAC20.149812734082397No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGGG1200.034.03
CCGCTAG1250.034.01
CGCTAGG1200.034.02
CTAGGGG600.034.04
TAGGGGA251.1207712E-434.05
CTAGGGT200.001888579233.9999964
CTAGGGC200.001888579233.9999964
CTAGGGA200.001888579233.9999964
TAGGGTC200.001888579233.9999965
Produced by FastQC (version 0.11.5)