##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526005_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1335 Sequences flagged as poor quality 0 Sequence length 40 %GC 55 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.83895131086142 29.0 4.0 30.0 4.0 33.0 2 16.483895131086143 14.0 4.0 29.0 4.0 32.0 3 9.765543071161048 6.0 4.0 14.0 4.0 21.0 4 13.352808988764044 9.0 4.0 23.0 4.0 29.0 5 10.549063670411986 6.0 4.0 15.0 4.0 24.0 6 11.083146067415731 7.0 4.0 17.0 4.0 24.0 7 13.093632958801498 10.0 4.0 22.0 4.0 27.0 8 12.209737827715356 5.0 4.0 20.0 4.0 26.0 9 12.578277153558053 5.0 4.0 20.0 4.0 27.0 10 10.02247191011236 4.0 4.0 14.0 4.0 23.0 11 15.731086142322097 4.0 4.0 30.0 4.0 34.0 12 15.789513108614232 4.0 4.0 30.0 4.0 33.0 13 15.265917602996256 4.0 4.0 29.0 4.0 33.0 14 15.783520599250936 4.0 4.0 30.0 4.0 34.0 15 14.74307116104869 4.0 4.0 29.0 4.0 34.0 16 12.798501872659177 4.0 4.0 24.0 4.0 32.0 17 12.808239700374532 4.0 4.0 24.0 4.0 31.0 18 12.472659176029962 4.0 4.0 23.0 4.0 31.0 19 11.578277153558053 4.0 4.0 20.0 4.0 31.0 20 11.791760299625468 4.0 4.0 22.0 4.0 31.0 21 10.722097378277153 4.0 4.0 17.0 4.0 31.0 22 10.334082397003746 4.0 4.0 15.0 4.0 30.0 23 9.808239700374532 4.0 4.0 13.0 4.0 29.0 24 9.561048689138577 4.0 4.0 10.0 4.0 29.0 25 9.650936329588015 4.0 4.0 10.0 4.0 30.0 26 8.631460674157303 4.0 4.0 4.0 4.0 28.0 27 8.80374531835206 4.0 4.0 4.0 4.0 29.0 28 8.304119850187266 4.0 4.0 4.0 4.0 27.0 29 7.828464419475655 4.0 4.0 4.0 4.0 25.0 30 7.586516853932585 4.0 4.0 4.0 4.0 24.0 31 7.614981273408239 4.0 4.0 4.0 4.0 26.0 32 7.370037453183521 4.0 4.0 4.0 4.0 24.0 33 7.456928838951311 4.0 4.0 4.0 4.0 25.0 34 7.522097378277153 4.0 4.0 4.0 4.0 26.0 35 7.331086142322097 4.0 4.0 4.0 4.0 25.0 36 7.69438202247191 4.0 4.0 4.0 4.0 29.0 37 7.555056179775281 4.0 4.0 4.0 4.0 29.0 38 7.572284644194757 4.0 4.0 4.0 4.0 29.0 39 7.5535580524344565 4.0 4.0 4.0 4.0 29.0 40 7.346067415730337 4.0 4.0 4.0 4.0 28.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 4.338405797101448 1 2 12.253623188405797 1 3 -0.04456521739130537 1 4 10.634420289855072 1 5 9.76159420289855 1 6 5.274275362318841 1 7 11.142753623188405 1 8 3.1358695652173907 1 9 12.592391304347826 1 10 5.842391304347826 1 11 5.918478260869566 1 12 6.7844202898550705 1 13 -2.4239130434782616 1 14 9.121739130434783 1 15 15.271376811594203 1 16 6.094202898550725 1 17 10.844927536231884 1 18 4.494927536231884 1 19 8.826086956521738 1 20 15.286594202898552 1 21 21.81340579710145 1 22 -7.263768115942028 1 23 -6.586594202898551 1 24 -5.76340579710145 1 25 -5.880072463768116 1 26 -4.727173913043478 1 27 -4.775 1 28 -4.199999999999999 1 29 -3.461956521739131 1 30 -2.9474637681159432 1 31 -3.4655797101449277 1 32 -3.5235507246376807 1 33 -3.5778985507246386 1 34 -3.4855072463768115 1 35 -3.5434782608695654 1 36 -4.282608695652174 1 37 -3.8967391304347823 1 38 -3.72463768115942 1 39 -3.7010869565217392 1 40 -3.4692028985507246 2 1 -17.661594202898552 2 2 -12.746376811594203 2 3 -6.044565217391305 2 4 -9.365579710144928 2 5 -6.23840579710145 2 6 -6.725724637681159 2 7 -8.857246376811595 2 8 -7.864130434782609 2 9 -8.407608695652174 2 10 -5.157608695652174 2 11 -13.081521739130434 2 12 -12.21557971014493 2 13 -11.423913043478262 2 14 -11.878260869565217 2 15 -11.728623188405797 2 16 -8.905797101449275 2 17 -9.155072463768116 2 18 -9.505072463768116 2 19 -7.173913043478262 2 20 -8.713405797101448 2 21 -7.186594202898551 2 22 -7.263768115942028 2 23 -6.586594202898551 2 24 -5.76340579710145 2 25 -5.880072463768116 2 26 -4.727173913043478 2 27 -4.775 2 28 -4.199999999999999 2 29 -3.461956521739131 2 30 -2.9474637681159432 2 31 -3.4655797101449277 2 32 -3.5235507246376807 2 33 -3.5778985507246386 2 34 -3.4855072463768115 2 35 -3.5434782608695654 2 36 -4.282608695652174 2 37 -3.8967391304347823 2 38 -3.72463768115942 2 39 -3.7010869565217392 2 40 -3.4692028985507246 5 1 8.338405797101448 5 2 -2.746376811594203 5 3 13.955434782608695 5 4 -1.865579710144928 5 5 16.26159420289855 5 6 11.274275362318841 5 7 3.642753623188405 5 8 2.1358695652173907 5 9 -7.407608695652174 5 10 2.842391304347826 5 11 -1.5815217391304337 5 12 -1.2155797101449295 5 13 -1.9239130434782616 5 14 -1.8782608695652172 5 15 -3.7286231884057965 5 16 0.5942028985507246 5 17 3.344927536231884 5 18 3.9949275362318843 5 19 6.326086956521738 5 20 5.786594202898552 5 21 -1.6865942028985508 5 22 -1.7637681159420282 5 23 2.413405797101449 5 24 5.73659420289855 5 25 6.619927536231884 5 26 9.772826086956522 5 27 10.225 5 28 8.8 5 29 9.53804347826087 5 30 10.052536231884057 5 31 12.034420289855072 5 32 11.97644927536232 5 33 11.922101449275361 5 34 13.514492753623188 5 35 11.956521739130434 5 36 11.217391304347826 5 37 8.603260869565219 5 38 8.27536231884058 5 39 5.298913043478261 5 40 12.530797101449275 6 1 7.338405797101448 6 2 -1.746376811594203 6 3 1.9554347826086946 6 4 -7.365579710144928 6 5 7.76159420289855 6 6 13.274275362318841 6 7 16.142753623188405 6 8 15.13586956521739 6 9 12.592391304347826 6 10 2.842391304347826 6 11 10.918478260869566 6 12 11.78442028985507 6 13 12.576086956521738 6 14 20.121739130434783 6 15 20.2713768115942 6 16 17.094202898550726 6 17 17.844927536231886 6 18 16.494927536231884 6 19 24.82608695652174 6 20 18.286594202898552 6 21 20.81340579710145 6 22 25.736231884057972 6 23 23.413405797101447 6 24 24.23659420289855 6 25 24.119927536231884 6 26 15.272826086956522 6 27 13.225 6 28 13.8 6 29 22.538043478260867 6 30 -2.9474637681159432 6 31 -3.4655797101449277 6 32 -3.5235507246376807 6 33 -3.5778985507246386 6 34 -3.4855072463768115 6 35 -3.5434782608695654 6 36 -4.282608695652174 6 37 -3.8967391304347823 6 38 -3.72463768115942 6 39 -3.7010869565217392 6 40 -3.4692028985507246 7 1 -2.8615942028985515 7 2 -3.746376811594203 7 3 -3.844565217391305 7 4 -7.765579710144928 7 5 -2.8384057971014496 7 6 -3.3257246376811587 7 7 -4.0572463768115945 7 8 -2.8641304347826093 7 9 -4.2076086956521745 7 10 -2.9576086956521737 7 11 -1.481521739130434 7 12 -1.6155797101449298 7 13 0.176086956521738 7 14 -1.0782608695652165 7 15 -5.328623188405796 7 16 -3.3057971014492757 7 17 -3.5550724637681164 7 18 -9.505072463768116 7 19 -7.173913043478262 7 20 -8.713405797101448 7 21 -7.186594202898551 7 22 -7.263768115942028 7 23 -6.586594202898551 7 24 -5.76340579710145 7 25 -5.880072463768116 7 26 -4.727173913043478 7 27 -4.775 7 28 -4.199999999999999 7 29 -3.461956521739131 7 30 -2.9474637681159432 7 31 -3.4655797101449277 7 32 -3.5235507246376807 7 33 -3.5778985507246386 7 34 -3.4855072463768115 7 35 -3.5434782608695654 7 36 -4.282608695652174 7 37 -3.8967391304347823 7 38 -3.72463768115942 7 39 -3.7010869565217392 7 40 -3.4692028985507246 8 1 9.838405797101448 8 2 14.753623188405797 8 3 -4.044565217391305 8 4 -3.865579710144928 8 5 -5.23840579710145 8 6 -4.725724637681159 8 7 -4.357246376811595 8 8 -1.8641304347826093 8 9 -4.907608695652174 8 10 -4.157608695652174 8 11 0.9184782608695663 8 12 1.7844202898550705 8 13 2.5760869565217384 8 14 2.121739130434783 8 15 1.7713768115942035 8 16 -8.905797101449275 8 17 -9.155072463768116 8 18 -9.505072463768116 8 19 -7.173913043478262 8 20 -8.713405797101448 8 21 -7.186594202898551 8 22 -7.263768115942028 8 23 -6.586594202898551 8 24 -5.76340579710145 8 25 -5.880072463768116 8 26 -4.727173913043478 8 27 -4.775 8 28 -4.199999999999999 8 29 -3.461956521739131 8 30 -2.9474637681159432 8 31 -3.4655797101449277 8 32 -3.5235507246376807 8 33 -3.5778985507246386 8 34 -3.4855072463768115 8 35 -3.5434782608695654 8 36 -4.282608695652174 8 37 -3.8967391304347823 8 38 -3.72463768115942 8 39 -3.7010869565217392 8 40 -3.4692028985507246 9 1 -5.161594202898552 9 2 -2.746376811594203 9 3 -2.5445652173913054 9 4 -1.365579710144928 9 5 -3.23840579710145 9 6 -5.225724637681159 9 7 -1.3572463768115952 9 8 -0.3641304347826093 9 9 -2.907608695652174 9 10 -0.15760869565217384 9 11 2.9184782608695663 9 12 0.2844202898550705 9 13 -0.9239130434782616 9 14 -3.878260869565217 9 15 1.7713768115942035 9 16 2.0942028985507246 9 17 -2.155072463768116 9 18 1.4949275362318843 9 19 -5.173913043478262 9 20 -0.2134057971014478 9 21 0.8134057971014492 9 22 1.7362318840579718 9 23 5.913405797101449 9 24 6.73659420289855 9 25 7.119927536231884 9 26 -4.727173913043478 9 27 -4.775 9 28 -4.199999999999999 9 29 -3.461956521739131 9 30 -2.9474637681159432 9 31 -3.4655797101449277 9 32 -3.5235507246376807 9 33 -3.5778985507246386 9 34 -3.4855072463768115 9 35 -3.5434782608695654 9 36 -4.282608695652174 9 37 -3.8967391304347823 9 38 -3.72463768115942 9 39 -3.7010869565217392 9 40 -3.4692028985507246 10 1 2.7384057971014464 10 2 1.253623188405797 10 3 -2.644565217391305 10 4 -4.165579710144929 10 5 -3.0384057971014498 10 6 -0.5257246376811597 10 7 -0.45724637681159486 10 8 -0.8641304347826093 10 9 -1.6076086956521731 10 10 1.6423913043478269 10 11 3.318478260869565 10 12 4.184420289855069 10 13 4.976086956521737 10 14 4.121739130434783 10 15 -2.928623188405796 10 16 1.494202898550725 10 17 -0.155072463768116 10 18 -1.705072463768115 10 19 -0.17391304347826164 10 20 -0.9134057971014471 10 21 2.8134057971014492 10 22 -3.6637681159420286 10 23 -2.7865942028985513 10 24 -1.36340579710145 10 25 -2.080072463768116 10 26 0.6728260869565226 10 27 -0.9750000000000005 10 28 1.200000000000001 10 29 -3.461956521739131 10 30 -2.9474637681159432 10 31 -3.4655797101449277 10 32 -3.5235507246376807 10 33 -3.5778985507246386 10 34 -3.4855072463768115 10 35 -3.5434782608695654 10 36 -4.282608695652174 10 37 -3.8967391304347823 10 38 -3.72463768115942 10 39 -3.7010869565217392 10 40 -3.4692028985507246 11 1 -4.661594202898552 11 2 -12.746376811594203 11 3 -6.044565217391305 11 4 -9.365579710144928 11 5 -6.23840579710145 11 6 -6.725724637681159 11 7 -8.857246376811595 11 8 -7.864130434782609 11 9 -8.407608695652174 11 10 -5.157608695652174 11 11 -13.081521739130434 11 12 -12.21557971014493 11 13 -11.423913043478262 11 14 -11.878260869565217 11 15 -11.728623188405797 11 16 -8.905797101449275 11 17 -9.155072463768116 11 18 -9.505072463768116 11 19 -7.173913043478262 11 20 -8.713405797101448 11 21 -7.186594202898551 11 22 -7.263768115942028 11 23 -6.586594202898551 11 24 -5.76340579710145 11 25 -5.880072463768116 11 26 -4.727173913043478 11 27 -4.775 11 28 -4.199999999999999 11 29 -3.461956521739131 11 30 -2.9474637681159432 11 31 -3.4655797101449277 11 32 -3.5235507246376807 11 33 -3.5778985507246386 11 34 -3.4855072463768115 11 35 -3.5434782608695654 11 36 -4.282608695652174 11 37 -3.8967391304347823 11 38 -3.72463768115942 11 39 -3.7010869565217392 11 40 -3.4692028985507246 12 1 -6.161594202898552 12 2 -1.746376811594203 12 3 -2.5445652173913054 12 4 2.134420289855072 12 5 2.76159420289855 12 6 -5.225724637681159 12 7 -0.8572463768115952 12 8 -2.3641304347826093 12 9 -0.40760869565217384 12 10 -1.1576086956521738 12 11 -2.0815217391304337 12 12 3.2844202898550705 12 13 -0.42391304347826164 12 14 -0.8782608695652172 12 15 -1.2286231884057965 12 16 3.0942028985507246 12 17 4.344927536231884 12 18 -0.5050724637681157 12 19 -0.6739130434782616 12 20 4.286594202898552 12 21 -7.186594202898551 12 22 -7.263768115942028 12 23 -6.586594202898551 12 24 -5.76340579710145 12 25 -5.880072463768116 12 26 -4.727173913043478 12 27 -4.775 12 28 -4.199999999999999 12 29 -3.461956521739131 12 30 -2.9474637681159432 12 31 -3.4655797101449277 12 32 -3.5235507246376807 12 33 -3.5778985507246386 12 34 -3.4855072463768115 12 35 -3.5434782608695654 12 36 -4.282608695652174 12 37 -3.8967391304347823 12 38 -3.72463768115942 12 39 -3.7010869565217392 12 40 -3.4692028985507246 13 1 -3.161594202898552 13 2 -9.746376811594203 13 3 5.455434782608695 13 4 0.13442028985507193 13 5 3.26159420289855 13 6 1.774275362318841 13 7 -8.857246376811595 13 8 -5.364130434782609 13 9 -4.907608695652174 13 10 -2.657608695652174 13 11 -3.0815217391304337 13 12 -3.2155797101449295 13 13 -5.423913043478262 13 14 -8.878260869565217 13 15 -8.728623188405797 13 16 -5.405797101449275 13 17 2.344927536231884 13 18 -1.5050724637681157 13 19 0.32608695652173836 13 20 1.2865942028985522 13 21 1.3134057971014492 13 22 -4.263768115942028 13 23 5.913405797101449 13 24 1.7365942028985497 13 25 -2.8800724637681157 13 26 9.272826086956522 13 27 10.225 13 28 8.8 13 29 9.03804347826087 13 30 12.052536231884057 13 31 10.034420289855072 13 32 8.47644927536232 13 33 9.922101449275361 13 34 5.5144927536231885 13 35 3.4565217391304346 13 36 12.217391304347826 13 37 11.603260869565219 13 38 8.77536231884058 13 39 11.798913043478262 13 40 12.030797101449275 14 1 12.338405797101448 14 2 17.253623188405797 14 3 14.955434782608695 14 4 20.634420289855072 14 5 15.76159420289855 14 6 15.274275362318841 14 7 17.142753623188405 14 8 18.13586956521739 14 9 20.592391304347828 14 10 9.842391304347826 14 11 17.918478260869566 14 12 9.78442028985507 14 13 19.57608695652174 14 14 14.121739130434783 14 15 4.2713768115942035 14 16 11.094202898550725 14 17 19.844927536231886 14 18 9.494927536231884 14 19 14.826086956521738 14 20 17.286594202898552 14 21 18.81340579710145 14 22 23.736231884057972 14 23 13.413405797101449 14 24 20.23659420289855 14 25 6.119927536231884 14 26 10.272826086956522 14 27 16.225 14 28 25.8 14 29 26.538043478260867 14 30 14.052536231884057 14 31 20.53442028985507 14 32 25.47644927536232 14 33 16.42210144927536 14 34 7.5144927536231885 14 35 -1.5434782608695654 14 36 24.717391304347828 14 37 -3.8967391304347823 14 38 -3.72463768115942 14 39 -3.7010869565217392 14 40 -3.4692028985507246 15 1 7.67173913043478 15 2 0.586956521739129 15 3 -4.044565217391305 15 4 4.30108695652174 15 5 -2.571739130434783 15 6 -0.3923913043478251 15 7 -0.5239130434782613 15 8 0.1358695652173907 15 9 -2.407608695652174 15 10 -1.8242753623188408 15 11 8.251811594202898 15 12 8.117753623188403 15 13 7.576086956521738 15 14 6.121739130434783 15 15 5.938043478260871 15 16 6.7608695652173925 15 17 2.844927536231884 15 18 4.494927536231884 15 19 1.4927536231884044 15 20 -0.04673913043478173 15 21 -1.1865942028985508 15 22 0.7362318840579718 15 23 0.08007246376811494 15 24 1.9032608695652158 15 25 2.7865942028985504 15 26 -4.727173913043478 15 27 -4.775 15 28 -4.199999999999999 15 29 -3.461956521739131 15 30 -2.9474637681159432 15 31 -3.4655797101449277 15 32 -3.5235507246376807 15 33 -3.5778985507246386 15 34 -3.4855072463768115 15 35 -3.5434782608695654 15 36 -4.282608695652174 15 37 -3.8967391304347823 15 38 -3.72463768115942 15 39 -3.7010869565217392 15 40 -3.4692028985507246 16 1 -4.661594202898552 16 2 -2.746376811594203 16 3 -5.044565217391305 16 4 -4.865579710144928 16 5 -2.23840579710145 16 6 -3.725724637681159 16 7 -1.3572463768115952 16 8 -0.3641304347826093 16 9 -2.907608695652174 16 10 -2.657608695652174 16 11 0.4184782608695663 16 12 1.2844202898550705 16 13 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112 38 -3.72463768115942 112 39 -3.7010869565217392 112 40 -3.4692028985507246 114 1 -4.661594202898552 114 2 -3.746376811594203 114 3 2.4554347826086946 114 4 3.134420289855072 114 5 -0.7384057971014499 114 6 -1.725724637681159 114 7 0.1427536231884048 114 8 -2.8641304347826093 114 9 1.5923913043478262 114 10 -0.6576086956521738 114 11 -6.581521739130434 114 12 2.7844202898550705 114 13 4.076086956521738 114 14 -1.3782608695652172 114 15 -1.2286231884057965 114 16 6.094202898550725 114 17 5.844927536231884 114 18 5.494927536231884 114 19 7.326086956521738 114 20 -4.213405797101448 114 21 6.313405797101449 114 22 -7.263768115942028 114 23 -6.586594202898551 114 24 -5.76340579710145 114 25 -5.880072463768116 114 26 -4.727173913043478 114 27 -4.775 114 28 -4.199999999999999 114 29 -3.461956521739131 114 30 -2.9474637681159432 114 31 -3.4655797101449277 114 32 -3.5235507246376807 114 33 -3.5778985507246386 114 34 -3.4855072463768115 114 35 -3.5434782608695654 114 36 -4.282608695652174 114 37 -3.8967391304347823 114 38 -3.72463768115942 114 39 -3.7010869565217392 114 40 -3.4692028985507246 118 1 -17.661594202898552 118 2 -12.746376811594203 118 3 -6.044565217391305 118 4 -9.365579710144928 118 5 -6.23840579710145 118 6 -6.725724637681159 118 7 -8.857246376811595 118 8 -7.864130434782609 118 9 -8.407608695652174 118 10 -5.157608695652174 118 11 -13.081521739130434 118 12 -12.21557971014493 118 13 -11.423913043478262 118 14 -11.878260869565217 118 15 -11.728623188405797 118 16 -8.905797101449275 118 17 -9.155072463768116 118 18 -9.505072463768116 118 19 -7.173913043478262 118 20 -8.713405797101448 118 21 -7.186594202898551 118 22 -7.263768115942028 118 23 -6.586594202898551 118 24 -5.76340579710145 118 25 -5.880072463768116 118 26 -4.727173913043478 118 27 -4.775 118 28 -4.199999999999999 118 29 -3.461956521739131 118 30 -2.9474637681159432 118 31 -3.4655797101449277 118 32 -3.5235507246376807 118 33 -3.5778985507246386 118 34 -3.4855072463768115 118 35 -3.5434782608695654 118 36 -4.282608695652174 118 37 -3.8967391304347823 118 38 -3.72463768115942 118 39 -3.7010869565217392 118 40 -3.4692028985507246 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 547.0 5 105.0 6 9.0 7 2.0 8 3.0 9 32.0 10 51.0 11 41.0 12 49.0 13 55.0 14 49.0 15 38.0 16 39.0 17 36.0 18 25.0 19 36.0 20 38.0 21 26.0 22 31.0 23 26.0 24 28.0 25 22.0 26 16.0 27 9.0 28 8.0 29 5.0 30 3.0 31 5.0 32 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 0.0 100.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 100.0 0.0 6 0.0 100.0 0.0 0.0 7 99.09909909909909 0.5255255255255256 0.37537537537537535 0.0 8 98.12734082397003 0.5243445692883896 0.599250936329588 0.7490636704119851 9 98.95131086142322 0.44943820224719105 0.37453183520599254 0.22471910112359553 10 48.764044943820224 28.164794007490634 12.434456928838951 10.636704119850188 11 33.40823970037453 24.9438202247191 23.220973782771537 18.426966292134832 12 44.41947565543071 9.887640449438202 18.876404494382022 26.816479400749067 13 26.06741573033708 25.54307116104869 20.524344569288388 27.86516853932584 14 27.790262172284645 21.79775280898876 26.741573033707866 23.670411985018728 15 26.06741573033708 29.73782771535581 26.516853932584272 17.677902621722847 16 35.28089887640449 17.228464419475657 27.86516853932584 19.625468164794007 17 29.962546816479403 21.94756554307116 25.617977528089884 22.47191011235955 18 33.70786516853933 19.850187265917604 25.0187265917603 21.423220973782772 19 20.40510127531883 18.37959489872468 16.729182295573892 44.4861215303826 20 25.0187265917603 23.89513108614232 26.666666666666668 24.419475655430713 21 25.168539325842698 19.700374531835205 32.50936329588015 22.621722846441948 22 17.528089887640448 17.303370786516854 33.78277153558052 31.38576779026217 23 30.112359550561795 22.09737827715356 24.868913857677903 22.92134831460674 24 25.692883895131086 21.423220973782772 29.812734082397004 23.071161048689138 25 23.25581395348837 21.905476369092273 22.80570142535634 32.03300825206302 26 18.045112781954884 21.804511278195488 31.127819548872182 29.022556390977446 27 22.172284644194757 12.808988764044942 34.6816479400749 30.337078651685395 28 14.382022471910114 22.621722846441948 34.756554307116104 28.239700374531836 29 19.475655430711612 22.92134831460674 32.43445692883895 25.168539325842698 30 16.729182295573892 12.678169542385595 41.785446361590395 28.80720180045011 31 18.16816816816817 14.78978978978979 40.46546546546546 26.576576576576578 32 30.786516853932582 10.262172284644194 38.50187265917603 20.44943820224719 33 20.224719101123593 15.730337078651685 44.71910112359551 19.325842696629213 34 14.756554307116104 11.235955056179774 51.61048689138576 22.397003745318354 35 17.528089887640448 16.853932584269664 44.04494382022472 21.573033707865168 36 15.505617977528091 16.029962546816478 43.97003745318352 24.49438202247191 37 23.76311844077961 14.46776611694153 37.55622188905547 24.212893553223388 38 15.730337078651685 14.232209737827715 44.569288389513105 25.468164794007492 39 19.325842696629213 17.00374531835206 41.49812734082397 22.172284644194757 40 14.756554307116104 16.928838951310862 37.97752808988764 30.337078651685395 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 1.5 22 3.0 23 3.0 24 7.5 25 12.0 26 11.5 27 11.0 28 11.0 29 16.0 30 21.0 31 20.5 32 20.0 33 20.0 34 22.0 35 24.0 36 34.5 37 45.0 38 45.0 39 52.5 40 60.0 41 69.0 42 78.0 43 78.0 44 71.0 45 64.0 46 70.0 47 76.0 48 76.0 49 90.5 50 105.0 51 92.0 52 79.0 53 79.0 54 79.0 55 79.0 56 80.0 57 81.0 58 81.0 59 78.0 60 75.0 61 77.0 62 79.0 63 79.0 64 91.5 65 104.0 66 100.5 67 97.0 68 97.0 69 97.5 70 98.0 71 77.0 72 56.0 73 56.0 74 42.0 75 28.0 76 24.5 77 21.0 78 21.0 79 16.0 80 11.0 81 7.0 82 3.0 83 3.0 84 2.0 85 1.0 86 1.5 87 2.0 88 2.0 89 2.0 90 2.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.22471910112359553 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.149812734082397 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.149812734082397 26 0.37453183520599254 27 0.0 28 0.0 29 0.0 30 0.149812734082397 31 0.22471910112359553 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0749063670411985 38 0.0 39 0.0 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 1335.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.98501872659176 #Duplication Level Percentage of deduplicated Percentage of total 1 96.09120521172639 88.38951310861424 2 2.8501628664495113 5.2434456928838955 3 0.40716612377850164 1.1235955056179776 4 0.32573289902280134 1.198501872659176 5 0.0 0.0 6 0.0 0.0 7 0.08143322475570033 0.5243445692883896 8 0.08143322475570033 0.599250936329588 9 0.08143322475570033 0.6741573033707865 >10 0.08143322475570033 2.247191011235955 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCGCTAGGGTCCAGAGTTCTTTGTCATTACCTTTATGTTA 30 2.247191011235955 No Hit CCGCTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT 9 0.6741573033707865 No Hit CCGCTAGGGATGAGATGGACTTAAAGGAATTGACGGGTAC 8 0.599250936329588 No Hit CCGCTAGGGTCCAGAGTTCTTTGTCATTACCTTTCTGTTA 7 0.5243445692883896 No Hit CCGCTAGGGATGAGATGGCCTTAAAGGAATTGACGGGTAC 4 0.299625468164794 No Hit CCGCTAGGGGGTTTTTTTTACCCCCCCTTTGGTTTTGGTT 4 0.299625468164794 No Hit CCGCTAGGGATGAGATGGACTTAAATGAATTGACGGGTAC 4 0.299625468164794 No Hit CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG 4 0.299625468164794 No Hit CCGCTAGGGCATGGTAAACGCATCGATTTTTTTTTTTTTT 3 0.22471910112359553 No Hit CCGCTAGGGACATTTGAAGAATATTCTTGGAATAATCTTG 3 0.22471910112359553 No Hit CCGCTAGGGATGAGATGGACTCAAAGGAATTGACGGGTAC 3 0.22471910112359553 No Hit CCGCTAGGGGAGCAGACTTTTCATTCCTCTGGTTCTGTGG 3 0.22471910112359553 No Hit CCGCTAGGGATGAGATGGCCTTAAATGAATTGACGGGTAC 3 0.22471910112359553 No Hit CCGCTAGGGAAGTGTCACGCAGAGTCGACTTTTTTTTTTT 2 0.149812734082397 No Hit CCGCTAGGGGCCGAATAGCCTCTCCACCCCCGCGGCCCGC 2 0.149812734082397 No Hit CCGCTAGGGGGGCAGTGGTGGCGCACGCCTTTAATCCCAG 2 0.149812734082397 No Hit CCGCTAGGGTGGTCACAGCGTCGACTTTTTTTTTTTTTTT 2 0.149812734082397 No Hit CCGCTAGGGTCCAGAGTTCTTTGTCCTTACCTTTATGTTA 2 0.149812734082397 No Hit CCGCTAGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2 0.149812734082397 No Hit CCGCTAGGGGGGCTGGAGCGATGGCTCAGCAGTTAAGAGC 2 0.149812734082397 No Hit CCGCTAGGGGAGTTTCTGTGTGATGTGTTCTGCGTGACGC 2 0.149812734082397 No Hit CCGCTAGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTC 2 0.149812734082397 No Hit CCGCTAGGGCTGAGATGGACTCAAAGGAATTGACGGGTAC 2 0.149812734082397 No Hit CCGCTAGGGATGAGATGGACTTACAGGAATTGACGGGTAC 2 0.149812734082397 No Hit CCGCTAGGGGAGCTCTGTCTTCTTCAAGATCCCTGCACTG 2 0.149812734082397 No Hit CCGCTAGGGGATTCGCTAAGGGATCTTGCAGCTATGGATG 2 0.149812734082397 No Hit CCGCTAGGGGACATCTTGTTTCTTTGTGTTGCCCTTTGCT 2 0.149812734082397 No Hit CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTACGCGC 2 0.149812734082397 No Hit CCGCTAGGGAAGAGTGGTTCAACGCAGAGTCGACTTTTTT 2 0.149812734082397 No Hit CCGCTAGGGTCAACGCAGCCGATTTTTTTTTTTTTTTTTT 2 0.149812734082397 No Hit CCGCTAGGGGACCCGCGGCAGCATGAAGAAGGTTTTCCCT 2 0.149812734082397 No Hit CCGCTAGGGATGAGATGGACTCACAGGAATTGACGGGTAC 2 0.149812734082397 No Hit CCGCTATGGCCTATAATCCTCTCACTGTAGCCTTATCCCA 2 0.149812734082397 No Hit CCGCTAGGGGCTGGTGAGATGGCTCAGTGGGTAAGCGCCC 2 0.149812734082397 No Hit CCGCTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT 2 0.149812734082397 No Hit CCGCTAGGGATGGTATCACCGCAGATCGACTTTTTTTTTT 2 0.149812734082397 No Hit CCGCTAGGGTCCAGAGTTCTTTGTCATTCCCTTTCTGTTA 2 0.149812734082397 No Hit CCGCTAGGGGGGAATTCCTTCGATTCTGTTTTGCTCCCCG 2 0.149812734082397 No Hit CCGCTAGGGGACCCGCGGCAGCATGACGACGGTTTTCCCT 2 0.149812734082397 No Hit CCGCTAGGGATGAGATGGCCTCAAATGAATTGACGGGTAC 2 0.149812734082397 No Hit CCGCTAGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT 2 0.149812734082397 No Hit CCGCTAGGGATGAGATGGACTCAAATGAATTGACGGGTAC 2 0.149812734082397 No Hit CCGCTAGGGGGGCTGGAGCGATGGCTCAGCGGTTAAGAGC 2 0.149812734082397 No Hit CCGCTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT 2 0.149812734082397 No Hit CCGCTAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAGAGC 2 0.149812734082397 No Hit CCGCTAGGGTACCTGGTTGATCCTGCCCGTAGCCTATGCT 2 0.149812734082397 No Hit CCGCTAGGGGAGCAGCCTTTTCATTCCTCTGGTTCTGTGG 2 0.149812734082397 No Hit CCGCTAGGGGCGGGGATGCGATGGACTTAAATGAATTGAC 2 0.149812734082397 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0749063670411985 0.0 0.0 0.0 0.0 17 0.0749063670411985 0.0 0.0 0.0 0.0 18 0.0749063670411985 0.0 0.0 0.0 0.0 19 0.0749063670411985 0.0 0.0 0.0 0.0 20 0.0749063670411985 0.0 0.0 0.0 0.0 21 0.0749063670411985 0.0 0.0 0.0 0.0 22 0.0749063670411985 0.0 0.0 0.0 0.0 23 0.0749063670411985 0.0 0.0 0.0 0.0 24 0.0749063670411985 0.0 0.0 0.0 0.0 25 0.0749063670411985 0.0 0.0 0.0 0.0 26 0.0749063670411985 0.0 0.0 0.0 0.0 27 0.0749063670411985 0.0 0.0 0.0 0.0 28 0.0749063670411985 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTAGGG 120 0.0 34.0 3 CCGCTAG 125 0.0 34.0 1 CGCTAGG 120 0.0 34.0 2 CTAGGGG 60 0.0 34.0 4 TAGGGGA 25 1.1207712E-4 34.0 5 CTAGGGT 20 0.0018885792 33.999996 4 CTAGGGC 20 0.0018885792 33.999996 4 CTAGGGA 20 0.0018885792 33.999996 4 TAGGGTC 20 0.0018885792 33.999996 5 >>END_MODULE