FastQCFastQC Report
Fri 17 Jun 2016
SRR1526002_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526002_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39482
Sequences flagged as poor quality0
Sequence length56
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTTCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC15013.8017324350336863No Hit
TACTTCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT14993.7966668355199835No Hit
TACTTCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC5891.4918190567853706No Hit
TACTTCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT2450.6205359404285498No Hit
TACTTCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA2180.5521503469935667No Hit
TACTTCGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC1970.498961552099691No Hit
TACTTCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC1870.4736335545311787No Hit
TACTTCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC1490.37738716377083226No Hit
TACTTCGGGTCACAGATCCTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCATC1240.3140671698495517No Hit
TACTTCGGGGCTTACTTCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATT970.24568157641456867No Hit
TACTTCGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCA950.2406159769008662No Hit
TACTTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT930.23555037738716378No Hit
TACTTCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT850.215287979332354No Hit
TACTTCGGGGCTTACTTCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAA840.21275517957550275No Hit
TACTTCGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCGGATTGAACAAAAACTC820.2076895800618003No Hit
TACTTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG660.16716478395218073No Hit
TACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA620.15703358492477584No Hit
TACTTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA580.14690238589737095No Hit
TACTTCGGGTCCCCCCTCCTCTTCTGAGATGAGTTTTTGTTCCCTCCGCCCAATCT540.13677118686996606No Hit
TACTTCGGGGCTTACTTCGGGTCACAGATCCTCTTCTGAGCTGAGTTTTTGTTCAA530.13423838711311484No Hit
TACTTCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTA530.13423838711311484No Hit
TACTTCGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGCTTGAACAAAAACTCA460.11650878881515626No Hit
TACTTCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAGAT450.11397598905830504No Hit
TACTTCGGCTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT450.11397598905830504No Hit
TACTTCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAAACT450.11397598905830504No Hit
TACTTCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAAAAA420.10637758978775137No Hit
TACTTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC410.10384479003090015No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCAATT150.00784622850.82383740
CACCGCG150.00784622850.82383739
TGATTGA150.00784622850.82383740
TCTAATT150.00784622850.82383740
CTAATTC150.00784622850.82383741
GCTTGCC150.00784622850.82383740
GCACTGA150.00784622850.82383740
CGCACTG150.00784622850.82383739
GCCTGGA150.00784622850.82383739
CCGCGGC150.00784622850.82383741
GTCTAAT251.86096E-550.82383339
TTAATCC203.8132124E-450.82383341
CCGATTG402.222805E-950.82383339
CGATTGA402.222805E-950.82383340
TGTCTAA251.8753131E-550.75808738
GCGCACT150.00788655950.75808738
GCACCGC150.00788655950.75808738
GCCGATT451.10958354E-1050.75808338
TTGTCTA251.904295E-550.62709837
TGCACCG150.007967683550.62709837
Produced by FastQC (version 0.11.5)