FastQCFastQC Report
Fri 17 Jun 2016
SRR1526001_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526001_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6527
Sequences flagged as poor quality0
Sequence length40
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTTCGGGCTCGTCTTAATCAGCAACATCTTTTGATACA160.24513559062356366No Hit
TACTTCGGGAATTGACGGGTACGCACAGTTCTGAGTCGGA130.19917266738164546No Hit
TACTTCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT130.19917266738164546No Hit
TACTTCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT100.1532097441397273No Hit
TACTTCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT90.13788876972575456No Hit
TACTTCGGGATCCAGTGACAGATACCATTAGATTCAAGAG70.1072468208978091No Hit
TACTTCGGGAAGCAGTGGTATCAACGCCGAGTCGACTTTT70.1072468208978091No Hit
TACTTCGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT70.1072468208978091No Hit
TACTTCGGGGAGTGGTTCTGTTTTGTTTTTGTTTTTTGTT70.1072468208978091No Hit
TACTTCGGGAATTGACGGGTACGCACCGTTCTGAGTCGGA70.1072468208978091No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGTG200.002435888234.4218756
CGGGGCT301.1644912E-534.4218756
CGGGGAG301.1644912E-534.4218756
CGGGCTC301.1644912E-534.4218756
GGGAGCA200.002435888234.4218757
CGGGAAG601.8189894E-1234.4218756
CTTCGGT251.6856326E-434.4218753
GGGGCTT301.1644912E-534.4218757
TTCGGGT405.5153578E-834.4218754
TTCGGAA200.002435888234.4218754
TCGGGTC200.002435888234.4218755
TCGGGGT358.026418E-734.4218755
CAACATC251.8498053E-433.89230724
GCAACAT200.002631498333.89230723
GGCTTAC200.002631498333.8923079
GTGGTAT200.002631498333.89230715
ATCAACG200.002631498333.89230720
CGTCTTA301.2982882E-533.89230712
AACATCT251.8498053E-433.89230725
GGGCTTA200.002631498333.8923078
Produced by FastQC (version 0.11.5)