##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526001_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6527
Sequences flagged as poor quality	0
Sequence length	40
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14034012563199	33.0	30.0	35.0	26.0	38.0
2	20.608089474490576	22.0	13.0	28.0	4.0	33.0
3	24.801593381339053	28.0	19.0	33.0	4.0	36.0
4	26.239313620346255	30.0	22.0	34.0	4.0	37.0
5	25.223839436188143	28.0	21.0	33.0	4.0	36.0
6	20.099586333690823	17.0	11.0	31.0	4.0	36.0
7	24.810939175731576	28.0	21.0	33.0	4.0	36.0
8	23.558296307645165	28.0	15.0	33.0	4.0	36.0
9	24.37643634135131	28.0	18.0	33.0	4.0	36.0
10	19.559981614830704	20.0	9.0	30.0	4.0	34.0
11	21.710740003064195	24.0	11.0	32.0	4.0	36.0
12	21.784127470507123	25.0	10.0	31.0	4.0	35.0
13	22.451968745212195	26.0	11.0	32.0	4.0	36.0
14	22.548031254787805	27.0	10.0	33.0	4.0	36.0
15	20.84970124099893	24.0	4.0	32.0	4.0	35.0
16	20.749961697563965	24.0	4.0	32.0	4.0	36.0
17	21.20790562279761	25.0	4.0	33.0	4.0	36.0
18	20.66232572391604	24.0	4.0	32.0	4.0	36.0
19	18.064807721771103	18.0	4.0	30.0	4.0	35.0
20	19.89949440784434	23.0	4.0	32.0	4.0	35.0
21	16.243909912670446	15.0	4.0	28.0	4.0	33.0
22	16.622031561207294	15.0	4.0	29.0	4.0	34.0
23	16.486287727899494	15.0	4.0	29.0	4.0	33.0
24	15.99984679025586	13.0	4.0	29.0	4.0	33.0
25	15.82074459935652	10.0	4.0	29.0	4.0	33.0
26	13.745671824728053	4.0	4.0	25.0	4.0	33.0
27	13.620039834533475	4.0	4.0	26.0	4.0	33.0
28	13.621418722230734	4.0	4.0	26.0	4.0	33.0
29	12.868699249272254	4.0	4.0	24.0	4.0	32.0
30	12.124865941473878	4.0	4.0	22.0	4.0	32.0
31	12.558909146621724	4.0	4.0	25.0	4.0	33.0
32	11.890301823195955	4.0	4.0	22.0	4.0	32.0
33	11.773096368929064	4.0	4.0	23.0	4.0	32.0
34	11.504672897196262	4.0	4.0	22.0	4.0	31.0
35	11.172054542668914	4.0	4.0	20.0	4.0	31.0
36	11.588478627240692	4.0	4.0	20.0	4.0	35.0
37	11.54450743067259	4.0	4.0	21.0	4.0	35.0
38	11.341504519687453	4.0	4.0	17.0	4.0	35.0
39	10.863643327715643	4.0	4.0	17.0	4.0	33.0
40	10.726214187222308	4.0	4.0	16.0	4.0	33.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-10.149038966084415
1	2	-15.670533469056199
1	3	-19.695659025318115
1	4	-20.6316791667928
1	5	-19.75116354604991
1	6	-13.28462543769362
1	7	-19.690224043633133
1	8	-18.67494611449157
1	9	-19.701131381245013
1	10	-14.34557292455019
1	11	-16.376583265219626
1	12	-14.596483314665136
1	13	-17.11492463344737
1	14	-16.87041207529844
1	15	-15.956318782227873
1	16	-14.434365937093213
1	17	-16.554120563779655
1	18	-16.11184361093452
1	19	-13.687980479116845
1	20	-15.09388235501872
1	21	-11.637834766243857
1	22	-10.47807640591731
1	23	-11.46823371073371
1	24	-11.005057984944347
1	25	-10.928270391224931
1	26	-7.34102572932118
1	27	-6.687200236632053
1	28	-7.021243062493059
1	29	-6.265792729997274
1	30	-6.544103598421781
1	31	-5.267534700148341
1	32	-4.923881812631815
1	33	-4.215993223947766
1	34	-4.226117834186018
1	35	-4.10236114895206
1	36	-3.664838570520385
1	37	-4.914552593870778
1	38	-3.685219982038161
1	39	-3.806878475060291
1	40	-3.396421911421905
3	1	1.0731832561378063
3	2	-1.4483112468339776
3	3	-2.3623256919847826
3	4	-1.9650125001261323
3	5	-0.7511635460499093
3	6	8.493152340084158
3	7	4.976442623033535
3	8	6.658387218841764
3	9	5.409979729866098
3	10	3.987760408783142
3	11	14.956750068113706
3	12	11.292405574223753
3	13	10.218408699885963
3	14	12.240699035812671
3	15	15.599236773327682
3	16	5.010078507351231
3	17	9.779212769553677
3	18	7.4437119446210325
3	19	-4.799091590227956
3	20	13.1283398672035
3	21	11.806609678200587
3	22	0.2997013718604684
3	23	6.865099622599622
3	24	17.328275348388985
3	25	-11.928270391224931
3	26	-9.45213684043229
3	27	-9.576089125520943
3	28	-9.799020840270837
3	29	-8.710237174441719
3	30	-8.099659153977337
3	31	-8.711979144592785
3	32	-8.257215145965148
3	33	-7.660437668392211
3	34	-7.670562278630463
3	35	-7.546805593396504
3	36	-7.442616348298163
3	37	-7.136774816093
3	38	-7.129664426482606
3	39	-6.917989586171402
3	40	-6.84086635586635
5	1	0.0731832561378063
5	2	4.301688753166022
5	3	0.6376743080152174
5	4	-6.465012500126132
5	5	2.9988364539500907
5	6	3.4931523400841584
5	7	5.476442623033535
5	8	1.1583872188417637
5	9	1.659979729866098
5	10	-1.0122395912168578
5	11	-2.043249931886294
5	12	3.2924055742237535
5	13	5.218408699885963
5	14	6.990699035812671
5	15	-2.150763226672318
5	16	2.0100785073512313
5	17	9.029212769553677
5	18	9.193711944621032
5	19	5.700908409772044
5	20	3.1283398672035005
5	21	9.056609678200587
5	22	7.299701371860468
5	23	10.865099622599622
5	24	7.078275348388985
5	25	11.321729608775069
5	26	-5.95213684043229
5	27	-6.576089125520943
5	28	-6.2990208402708365
5	29	-7.210237174441719
5	30	-1.5996591539773366
5	31	-8.711979144592785
5	32	-8.257215145965148
5	33	-7.660437668392211
5	34	-7.670562278630463
5	35	-7.546805593396504
5	36	-7.442616348298163
5	37	-7.136774816093
5	38	-7.129664426482606
5	39	-6.917989586171402
5	40	-6.84086635586635
6	1	-6.926816743862194
6	2	-1.8768826754054047
6	3	0.20910287944379036
6	4	-5.107869642983275
6	5	-5.322592117478482
6	6	-5.649704802772984
6	7	-2.1664145198236078
6	8	-3.4844699240153787
6	9	1.981408301294671
6	10	0.7020461230688575
6	11	-4.3289642176005785
6	12	-3.1361658543476736
6	13	-0.21016272868546437
6	14	1.097841892955529
6	15	1.0278082018991093
6	16	-7.704207206934482
6	17	-1.0779300875891806
6	18	-0.5562880553789675
6	19	3.3437655526291863
6	20	4.414054152917785
6	21	2.235181106772014
6	22	8.728272800431895
6	23	8.436528194028195
6	24	5.756846776960412
6	25	7.500301037346496
6	26	10.976434588139137
6	27	7.99533944590763
6	28	10.058122016872021
6	29	13.861191396986854
6	30	2.18605513173695
6	31	2.0023065696929283
6	32	2.3142134254634232
6	33	2.4824194744649315
6	34	4.186580578512395
6	35	0.16748012088920916
6	36	-1.1569020625838764
6	37	1.5775108981927133
6	38	1.727478430660252
6	39	-1.7751324433142592
6	40	-1.412294927294921
7	1	5.448183256137806
7	2	8.364188753166022
7	3	10.075174308015217
7	4	10.472487499873868
7	5	10.81133645395009
7	6	13.430652340084158
7	7	8.351442623033535
7	8	9.783387218841764
7	9	7.972479729866098
7	10	13.300260408783142
7	11	7.456750068113706
7	12	7.6674055742237535
7	13	10.030908699885963
7	14	6.928199035812671
7	15	9.849236773327682
7	16	5.635078507351231
7	17	6.341712769553677
7	18	1.5687119446210325
7	19	5.700908409772044
7	20	8.2533398672035
7	21	8.056609678200587
7	22	10.612201371860468
7	23	12.240099622599622
7	24	7.765775348388985
7	25	8.946729608775069
7	26	5.61036315956771
7	27	-0.013589125520942602
7	28	-1.6115208402708365
7	29	-0.022737174441719077
7	30	0.1503408460226634
7	31	-3.274479144592785
7	32	-3.694715145965148
7	33	-3.160437668392211
7	34	-4.733062278630463
7	35	-5.796805593396504
7	36	-7.442616348298163
7	37	-7.136774816093
7	38	-7.129664426482606
7	39	-6.917989586171402
7	40	-6.84086635586635
8	1	4.573183256137806
8	2	2.8850220864993545
8	3	5.637674308015217
8	4	5.701654166540536
8	5	6.082169787283423
8	6	15.826485673417494
8	7	9.476442623033535
8	8	12.658387218841764
8	9	10.409979729866098
8	10	8.487760408783142
8	11	10.456750068113706
8	12	11.792405574223753
8	13	12.718408699885963
8	14	5.407365702479339
8	15	6.932570106661014
8	16	2.676745174017899
8	17	-3.054120563779655
8	18	2.1103786112877003
8	19	1.8675750764387118
8	20	1.7950065338701684
8	21	4.806609678200587
8	22	4.633034705193801
8	23	8.03176628926629
8	24	4.828275348388985
8	25	-1.261603724558265
8	26	4.7145298262343776
8	27	3.2572442078123895
8	28	0.8676458263958295
8	29	1.6230961588916148
8	30	-0.2663258206440027
8	31	2.1213541887405487
8	32	3.076118187368186
8	33	2.672895664941123
8	34	1.662771054702871
8	35	3.7865277399368296
8	36	6.890716985035169
8	37	3.1965585172403337
8	38	4.203668906850728
8	39	7.915343747161931
8	40	7.992466977466982
9	1	4.073183256137806
9	2	4.551688753166022
9	3	10.637674308015217
9	4	0.03498749987386773
9	5	-1.7511635460499093
9	6	16.49315234008416
9	7	10.976442623033535
9	8	12.658387218841764
9	9	11.409979729866098
9	10	16.987760408783142
9	11	11.956750068113706
9	12	10.292405574223753
9	13	9.218408699885963
9	14	-3.7593009641873287
9	15	15.599236773327682
9	16	8.010078507351231
9	17	11.779212769553677
9	18	11.443711944621032
9	19	12.200908409772044
9	20	14.1283398672035
9	21	18.806609678200587
9	22	21.29970137186047
9	23	21.865099622599622
9	24	22.328275348388985
9	25	22.07172960877507
9	26	4.54786315956771
9	27	19.423910874479056
9	28	9.200979159729163
9	29	17.28976282555828
9	30	5.900340846022663
9	31	12.288020855407215
9	32	12.742784854034852
9	33	18.339562331607787
9	34	3.329437721369537
9	35	21.453194406603494
9	36	-7.442616348298163
9	37	-7.136774816093
9	38	-7.129664426482606
9	39	-6.917989586171402
9	40	-6.84086635586635
10	1	2.273183256137809
10	2	4.685022086499355
10	3	-0.29565902531811616
10	4	4.834987499873868
10	5	5.782169787283426
10	6	5.959819006750823
10	7	9.909775956366865
10	8	9.45838721884176
10	9	10.209979729866095
10	10	11.054427075449809
10	11	11.956750068113706
10	12	11.759072240890422
10	13	11.885075366552627
10	14	12.840699035812673
10	15	10.065903439994347
10	16	11.343411840684567
10	17	13.045879436220343
10	18	12.910378611287701
10	19	15.000908409772041
10	20	12.061673200536834
10	21	9.406609678200589
10	22	15.89970137186047
10	23	14.131766289266288
10	24	14.79494201505565
10	25	10.338396275441735
10	26	12.54786315956771
10	27	8.023910874479059
10	28	9.000979159729164
10	29	10.156429492224948
10	30	7.500340846022665
10	31	8.288020855407215
10	32	1.3427848540348517
10	33	2.4062289982744556
10	34	1.996104388036203
10	35	4.053194406603495
10	36	1.4240503183685043
10	37	3.1298918505736673
10	38	4.470335573517394
10	39	4.348677080495266
10	40	4.15913364413365
12	1	2.962072145026692
12	2	4.551688753166022
12	3	5.304340974681885
12	4	5.701654166540536
12	5	4.359947565061201
12	6	-0.28462543769362014
12	7	4.643109289700202
12	8	8.547276107730653
12	9	4.187757507643877
12	10	11.098871519894253
12	11	6.8456389570025955
12	12	6.736850018668196
12	13	3.440630922108184
12	14	8.685143480257114
12	15	6.377014551105461
12	16	11.676745174017896
12	17	11.112546102887013
12	18	13.777045277954368
12	19	14.867575076438708
12	20	13.683895422759058
12	21	11.917720789311698
12	22	16.855256927416026
12	23	8.976210733710733
12	24	11.883830903944542
12	25	16.516174053219512
12	26	3.9923076040121526
12	27	6.201688652256836
12	28	8.978756937506942
12	29	3.9564294922249488
12	30	3.4558964015782188
12	31	2.3991319665183255
12	32	0.9650070762570735
12	33	4.4506734427189
12	34	1.996104388036203
12	35	0.6754166288257171
12	36	-2.5537274594092736
12	37	-3.3589970383152226
12	38	-2.351886648704827
12	39	-1.3624340306158462
12	40	-1.3964219114219052
13	1	2.216040398994952
13	2	-3.3054541039768353
13	3	-4.505182834841925
13	4	-6.250726785840417
13	5	-6.179734974621336
13	6	5.207438054369874
13	7	-4.737843091252181
13	8	-4.484469924015379
13	9	-6.590020270133902
13	10	1.5591889802117151
13	11	1.6710357823994215
13	12	1.8638341456523264
13	13	-0.3530198715426103
13	14	0.6692704643840983
13	15	2.7420939161848246
13	16	4.438649935922658
13	17	3.6363556266965347
13	18	5.586569087478175
13	19	-0.5133773045136714
13	20	2.4140541529177852
13	21	-8.764818893227984
13	22	-11.700298628139532
13	23	-9.992043234543235
13	24	-8.243153223039586
13	25	-10.35684181979636
13	26	-6.45213684043229
13	27	-7.004660554092371
13	28	-5.94187798312798
13	29	-5.853094317298862
13	30	-5.813944868263051
13	31	-4.569122001735643
13	32	-5.257215145965148
13	33	-3.660437668392211
13	34	-6.813419421487606
13	35	-3.975377021967933
13	36	-2.7283306340124494
13	37	-3.2796319589501435
13	38	-3.272521569339749
13	39	-3.060846729028545
13	40	-2.983723498723493
14	1	3.7398499228044706
14	2	6.551688753166022
14	3	7.971007641348553
14	4	7.368320833207203
14	5	6.248836453950091
14	6	9.159819006750826
14	7	8.643109289700199
14	8	4.658387218841764
14	9	8.743313063199434
14	10	12.321093742116474
14	11	10.290083401447038
14	12	11.959072240890418
14	13	4.218408699885963
14	14	11.907365702479336
14	15	4.26590343999435
14	16	3.676745174017899
14	17	-1.8874538971129908
14	18	10.1103786112877
14	19	-3.465758256894622
14	20	12.795006533870165
14	21	-3.5267236551327468
14	22	1.9663680385271363
14	23	4.865099622599622
14	24	-3.338391318277681
14	25	3.738396275441737
14	26	-3.4521368404322903
14	27	-4.242755792187609
14	28	-0.7990208402708365
14	29	-8.710237174441719
14	30	-8.099659153977337
14	31	-8.711979144592785
14	32	-8.257215145965148
14	33	-7.660437668392211
14	34	-7.670562278630463
14	35	-7.546805593396504
14	36	-7.442616348298163
14	37	-7.136774816093
14	38	-7.129664426482606
14	39	-6.917989586171402
14	40	-6.84086635586635
16	1	-2.9268167438621937
16	2	-1.9483112468339776
16	3	-0.3623256919847826
16	4	2.7849874998738677
16	5	0.9988364539500907
16	6	-3.5068476599158416
16	7	0.9764426230335346
16	8	5.658387218841764
16	9	3.159979729866098
16	10	6.237760408783142
16	11	4.206750068113706
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114	25	8.271729608775068
114	26	-0.252136840432291
114	27	3.223910874479058
114	28	-0.7990208402708365
114	29	2.6897628255582813
114	30	7.900340846022663
114	31	0.08802085540721549
114	32	3.5427848540348528
114	33	2.7395623316077895
114	34	5.129437721369538
114	35	5.053194406603497
114	36	4.357383651701838
114	37	5.063225183906999
114	38	0.8703355735173943
114	39	4.682010413828598
114	40	2.75913364413365
115	1	4.073183256137806
115	2	3.5516887531660224
115	3	-7.362325691984783
115	4	9.034987499873868
115	5	10.24883645395009
115	6	2.4931523400841584
115	7	9.976442623033535
115	8	11.658387218841764
115	9	10.409979729866098
115	10	-12.012239591216858
115	11	7.956750068113706
115	12	-6.7075944257762465
115	13	-2.7815913001140373
115	14	7.240699035812671
115	15	11.599236773327682
115	16	14.010078507351231
115	17	13.779212769553677
115	18	14.443711944621032
115	19	7.200908409772044
115	20	-4.8716601327964995
115	21	18.806609678200587
115	22	16.29970137186047
115	23	16.865099622599622
115	24	17.328275348388985
115	25	11.071729608775069
115	26	12.54786315956771
115	27	15.423910874479057
115	28	10.200979159729163
115	29	22.28976282555828
115	30	16.900340846022665
115	31	15.288020855407215
115	32	13.742784854034852
115	33	0.33956233160778915
115	34	15.329437721369537
115	35	3.4531944066034956
115	36	-3.442616348298163
115	37	-4.136774816093
115	38	11.870335573517394
115	39	22.0820104138286
115	40	22.15913364413365
116	1	-0.126816743862193
116	2	4.151688753166024
116	3	-2.1623256919847833
116	4	-4.965012500126132
116	5	1.44883645395009
116	6	-8.106847659915841
116	7	-0.6235573769664668
116	8	-3.741612781158235
116	9	-1.590020270133902
116	10	3.987760408783142
116	11	-4.643249931886295
116	12	-2.907594425776246
116	13	2.0184086998859634
116	14	1.2406990358126713
116	15	1.1992367733276836
116	16	0.21007850735123057
116	17	-0.6207872304463216
116	18	2.243711944621033
116	19	-9.399091590227956
116	20	-1.0716601327964987
116	21	-1.9933903217994136
116	22	-3.7002986281395316
116	23	-0.534900377400378
116	24	2.328275348388985
116	25	5.271729608775068
116	26	2.5478631595677097
116	27	7.623910874479057
116	28	3.800979159729165
116	29	2.0897628255582816
116	30	-0.49965915397733696
116	31	2.288020855407215
116	32	-2.0572151459651487
116	33	2.13956233160779
116	34	3.329437721369537
116	35	0.45319440660349564
116	36	3.357383651701838
116	37	4.263225183907
116	38	-1.529664426482606
116	39	1.0820104138285984
116	40	1.1591336441336502
117	1	1.0731832561378063
117	2	7.551688753166022
117	3	-11.362325691984783
117	4	6.034987499873868
117	5	6.248836453950091
117	6	-8.506847659915842
117	7	4.976442623033535
117	8	-7.341612781158236
117	9	4.409979729866098
117	10	-3.012239591216858
117	11	-1.0432499318862938
117	12	-4.7075944257762465
117	13	-14.781591300114037
117	14	-7.759300964187329
117	15	-1.4007632266723178
117	16	6.010078507351231
117	17	8.779212769553677
117	18	9.443711944621032
117	19	1.200908409772044
117	20	6.1283398672035005
117	21	10.806609678200587
117	22	16.29970137186047
117	23	-12.134900377400378
117	24	-11.671724651611015
117	25	-11.928270391224931
117	26	-9.45213684043229
117	27	-9.576089125520943
117	28	-9.799020840270837
117	29	-8.710237174441719
117	30	-8.099659153977337
117	31	-8.711979144592785
117	32	-8.257215145965148
117	33	-7.660437668392211
117	34	-7.670562278630463
117	35	-7.546805593396504
117	36	-7.442616348298163
117	37	-7.136774816093
117	38	-7.129664426482606
117	39	-6.917989586171402
117	40	-6.84086635586635
118	1	5.073183256137806
118	2	12.551688753166022
118	3	11.637674308015217
118	4	7.034987499873868
118	5	11.24883645395009
118	6	-1.5068476599158416
118	7	4.976442623033535
118	8	6.658387218841764
118	9	10.409979729866098
118	10	-7.012239591216858
118	11	1.9567500681137062
118	12	-0.7075944257762465
118	13	7.218408699885963
118	14	-11.759300964187329
118	15	-9.400763226672318
118	16	15.010078507351231
118	17	14.779212769553677
118	18	15.443711944621032
118	19	18.200908409772044
118	20	11.1283398672035
118	21	5.806609678200587
118	22	-1.7002986281395316
118	23	13.865099622599622
118	24	-1.6717246516110151
118	25	17.07172960877507
118	26	-9.45213684043229
118	27	-9.576089125520943
118	28	-9.799020840270837
118	29	-8.710237174441719
118	30	-8.099659153977337
118	31	-8.711979144592785
118	32	-8.257215145965148
118	33	-7.660437668392211
118	34	-7.670562278630463
118	35	-7.546805593396504
118	36	-7.442616348298163
118	37	-7.136774816093
118	38	-7.129664426482606
118	39	-6.917989586171402
118	40	-6.84086635586635
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	828.0
5	44.0
6	124.0
7	165.0
8	89.0
9	88.0
10	133.0
11	165.0
12	180.0
13	261.0
14	249.0
15	281.0
16	247.0
17	283.0
18	266.0
19	244.0
20	251.0
21	276.0
22	267.0
23	317.0
24	306.0
25	298.0
26	289.0
27	250.0
28	201.0
29	127.0
30	121.0
31	82.0
32	37.0
33	32.0
34	17.0
35	4.0
36	4.0
37	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	0.0	100.0
7	99.00291455744747	0.628930817610063	0.2761159687068569	0.09203865623561897
8	96.76628352490422	1.2260536398467434	0.8122605363984674	1.1954022988505748
9	93.11349693251533	3.8650306748466257	1.6717791411042946	1.3496932515337423
10	32.13793103448276	39.632183908045974	12.50574712643678	15.724137931034482
11	23.962003983453346	33.27715642714877	22.353301669986212	20.407537919411673
12	33.88999540370767	18.14003370614371	24.207139574076912	23.762831316071704
13	25.019151218017466	20.698636433277155	28.634901179715026	25.64731116899035
14	22.261375823502373	30.350850314079974	24.559521985598284	22.828251876819365
15	26.52060671058679	24.406312241458554	26.27547111996323	22.79760992799142
16	24.176497625248967	21.204228588938257	30.258924467596138	24.360349318216638
17	26.643174505898575	23.395127930136354	24.957867320361572	25.003830243603492
18	27.28665543128543	24.74337367856596	22.000919258464837	25.969051631683776
19	21.19021989850838	17.053667538059358	17.868675995694293	43.88743656773797
20	26.137582350237476	24.253102497318828	25.31024973188295	24.299065420560748
21	22.322659721158267	23.670905469587865	25.831162861958017	28.175271947295847
22	19.748736019610845	21.250191512180177	22.215412900260457	36.78565956794852
23	26.90363107093611	24.176497625248967	25.32557070629692	23.594300597518004
24	19.411674582503448	24.329707369388693	25.861804810785966	30.396813237321897
25	21.801746591083194	25.7851999387161	25.555385322506513	26.85766814769419
26	18.97720271102896	24.52248921749846	29.097350585335796	27.402957486136785
27	18.63030488739084	21.00505592155661	31.101578060364638	29.263061130687912
28	18.68199233716475	22.804597701149426	32.87356321839081	25.63984674329502
29	17.19852715556919	19.990794722307456	36.66768947529917	26.14298864682418
30	15.158023933722001	20.098189628720466	38.017796870205586	26.72598956735195
31	16.21663086836453	21.310217858238722	39.352562135624424	23.120589137772324
32	15.2183908045977	18.20689655172414	42.72796934865901	23.846743295019156
33	15.121801746591082	18.385169296767273	43.11322200091926	23.379806955722383
34	12.4712731729738	22.000919258464837	44.675961391144476	20.85184617741688
35	14.891987130381493	20.974413972728666	42.99065420560748	21.142944691282363
36	12.180174659108319	21.93963536080895	45.97824421633216	19.901945763750575
37	10.024524831391785	20.263641937461678	44.711833231146535	25.0
38	12.226137582350237	21.326796384250038	43.925233644859816	22.521832388539913
39	14.095296460854911	21.020376895970582	43.09790102650528	21.78642561666922
40	10.341657729431592	24.02328788110924	44.84449210969817	20.790562279760994
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	1.0
9	1.5
10	2.0
11	4.0
12	6.0
13	6.0
14	17.5
15	29.0
16	33.0
17	37.0
18	37.0
19	49.5
20	62.0
21	72.0
22	82.0
23	82.0
24	99.0
25	116.0
26	143.5
27	171.0
28	171.0
29	183.0
30	195.0
31	227.5
32	260.0
33	260.0
34	307.5
35	355.0
36	371.0
37	387.0
38	387.0
39	411.0
40	435.0
41	445.5
42	456.0
43	456.0
44	458.5
45	461.0
46	439.5
47	418.0
48	418.0
49	410.0
50	402.0
51	423.5
52	445.0
53	445.0
54	462.0
55	479.0
56	476.0
57	473.0
58	473.0
59	426.5
60	380.0
61	332.5
62	285.0
63	285.0
64	249.5
65	214.0
66	190.0
67	166.0
68	166.0
69	141.5
70	117.0
71	84.5
72	52.0
73	52.0
74	42.0
75	32.0
76	18.0
77	4.0
78	4.0
79	3.5
80	3.0
81	2.0
82	1.0
83	1.0
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.12256779531178183
8	0.030641948827945457
9	0.1072468208978091
10	0.030641948827945457
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.36770338593534546
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.5362341044890455
27	0.0
28	0.030641948827945457
29	0.13788876972575456
30	0.13788876972575456
31	0.13788876972575456
32	0.030641948827945457
33	0.0
34	0.0
35	0.0
36	0.0
37	0.04596292324191818
38	0.0
39	0.0
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	6527.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.77554772483529
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.81538958939541	91.75731576528267
2	2.3440025864856127	4.443082580052091
3	0.42030391205948914	1.1950360042898729
4	0.1454898157129001	0.5515550789030182
5	0.06466214031684449	0.3064194882794546
6	0.04849660523763337	0.2757775394515091
7	0.0808276753960556	0.5362341044890455
8	0.0	0.0
9	0.016165535079211122	0.13788876972575456
>10	0.06466214031684449	0.7966906695265819
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TACTTCGGGCTCGTCTTAATCAGCAACATCTTTTGATACA	16	0.24513559062356366	No Hit
TACTTCGGGAATTGACGGGTACGCACAGTTCTGAGTCGGA	13	0.19917266738164546	No Hit
TACTTCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT	13	0.19917266738164546	No Hit
TACTTCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT	10	0.1532097441397273	No Hit
TACTTCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT	9	0.13788876972575456	No Hit
TACTTCGGGATCCAGTGACAGATACCATTAGATTCAAGAG	7	0.1072468208978091	No Hit
TACTTCGGGAAGCAGTGGTATCAACGCCGAGTCGACTTTT	7	0.1072468208978091	No Hit
TACTTCGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	7	0.1072468208978091	No Hit
TACTTCGGGGAGTGGTTCTGTTTTGTTTTTGTTTTTTGTT	7	0.1072468208978091	No Hit
TACTTCGGGAATTGACGGGTACGCACCGTTCTGAGTCGGA	7	0.1072468208978091	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.015320974413972729	0.0	0.0	0.0	0.0
17	0.015320974413972729	0.0	0.0	0.0	0.0
18	0.015320974413972729	0.0	0.0	0.0	0.0
19	0.015320974413972729	0.0	0.0	0.0	0.0
20	0.015320974413972729	0.0	0.0	0.0	0.0
21	0.015320974413972729	0.0	0.0	0.0	0.0
22	0.015320974413972729	0.0	0.0	0.0	0.0
23	0.015320974413972729	0.0	0.0	0.0	0.0
24	0.015320974413972729	0.0	0.0	0.0	0.0
25	0.030641948827945457	0.0	0.0	0.0	0.0
26	0.030641948827945457	0.0	0.0	0.0	0.0
27	0.030641948827945457	0.0	0.0	0.0	0.0
28	0.030641948827945457	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGGTG	20	0.0024358882	34.421875	6
CGGGGCT	30	1.1644912E-5	34.421875	6
CGGGGAG	30	1.1644912E-5	34.421875	6
CGGGCTC	30	1.1644912E-5	34.421875	6
GGGAGCA	20	0.0024358882	34.421875	7
CGGGAAG	60	1.8189894E-12	34.421875	6
CTTCGGT	25	1.6856326E-4	34.421875	3
GGGGCTT	30	1.1644912E-5	34.421875	7
TTCGGGT	40	5.5153578E-8	34.421875	4
TTCGGAA	20	0.0024358882	34.421875	4
TCGGGTC	20	0.0024358882	34.421875	5
TCGGGGT	35	8.026418E-7	34.421875	5
CAACATC	25	1.8498053E-4	33.892307	24
GCAACAT	20	0.0026314983	33.892307	23
GGCTTAC	20	0.0026314983	33.892307	9
GTGGTAT	20	0.0026314983	33.892307	15
ATCAACG	20	0.0026314983	33.892307	20
CGTCTTA	30	1.2982882E-5	33.892307	12
AACATCT	25	1.8498053E-4	33.892307	25
GGGCTTA	20	0.0026314983	33.892307	8
>>END_MODULE