FastQCFastQC Report
Fri 17 Jun 2016
SRR1526000_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526000_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2543
Sequences flagged as poor quality0
Sequence length40
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTTCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT150.5898545025560362No Hit
TACTTCGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.31458906802988595No Hit
TACTTCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT80.31458906802988595No Hit
TACTTCGGGGGGCTGGTGAGATGGCCCAGTGGGTAAGAGC70.2752654345261502No Hit
TACTTCGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.23594180102241447No Hit
TACTTCGGGGAGTCGCCTTTTTTTTTTTTTTTTTTTTTTT50.19661816751867872No Hit
TACTTCGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.19661816751867872No Hit
TACTTCGGGGTCGCCTTTTTTTTTTTTTTTTTTTTTTTTT40.15729453401494298No Hit
TACTTCGGGTTCGCTCGACTTTTTTTTTTTTTTTTTTTTT40.15729453401494298No Hit
TACTTCGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.15729453401494298No Hit
TACTTCGGGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.11797090051120723No Hit
TACTTCGGGGCTTACTTCGGGAAGCATGGTATCAACGCAA30.11797090051120723No Hit
TACTTCGGGGGGCCGGTGAGATGGCCCAGTGGGTAAGAGC30.11797090051120723No Hit
TACTTCGGGGGGGCTGGTGAGATGGCTCCGTGGGTCCGCC30.11797090051120723No Hit
TACTTCGGGGCGTCGACTTTTTTTTTTTTTTTTTTTTTTT30.11797090051120723No Hit
TACTTCGGGGGGCTGGTGAGATGGCCCCGTGGGTAAGAGC30.11797090051120723No Hit
TACTTCGGGTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.11797090051120723No Hit
TACTTCGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTCT30.11797090051120723No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGGG251.3971509E-434.4081655
TCGGGGA251.3971509E-434.4081655
TCGGGAT251.3971509E-434.4081655
TTCGGGT355.606944E-734.408164
TCGGGTG200.002152710534.408165
ACTTCGG2450.032.301542
TACTTCG2450.032.301541
CTTCGGG2350.031.479813
TTCGGGC553.4742698E-1031.280154
TTCGGGA800.030.1071434
TTCGGGG703.6379788E-1229.492714
TCGGGAA304.0816318E-428.673475
CGGGAAG250.006428296727.5265316
Produced by FastQC (version 0.11.5)