##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526000_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2543 Sequences flagged as poor quality 0 Sequence length 40 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 13.357058592213921 4.0 4.0 26.0 4.0 30.0 2 9.267007471490366 4.0 4.0 14.0 4.0 23.0 3 7.138812426268188 4.0 4.0 8.0 4.0 14.0 4 10.080220212347621 4.0 4.0 19.0 4.0 26.0 5 9.771922925678332 4.0 4.0 19.0 4.0 25.0 6 6.699567440031459 4.0 4.0 8.0 4.0 13.0 7 9.149823043649233 4.0 4.0 19.0 4.0 22.0 8 9.873377900117971 4.0 4.0 20.0 4.0 27.0 9 9.173023987416437 4.0 4.0 16.0 4.0 25.0 10 7.506488399528116 4.0 4.0 9.0 4.0 19.0 11 8.000393236335038 4.0 4.0 9.0 4.0 22.0 12 8.08651199370822 4.0 4.0 10.0 4.0 22.0 13 8.30515139598899 4.0 4.0 10.0 4.0 22.0 14 8.038143924498623 4.0 4.0 8.0 4.0 22.0 15 7.873377900117971 4.0 4.0 6.0 4.0 21.0 16 8.250491545418797 4.0 4.0 4.0 4.0 22.0 17 7.840346047974833 4.0 4.0 4.0 4.0 21.0 18 7.664176169878097 4.0 4.0 4.0 4.0 20.0 19 7.893039716869839 4.0 4.0 4.0 4.0 22.0 20 8.055839559575304 4.0 4.0 4.0 4.0 22.0 21 8.370428627605191 4.0 4.0 4.0 4.0 24.0 22 8.124655918206843 4.0 4.0 4.0 4.0 23.0 23 8.356272119543846 4.0 4.0 4.0 4.0 24.0 24 8.175383405426661 4.0 4.0 4.0 4.0 23.0 25 8.30357845064884 4.0 4.0 4.0 4.0 23.0 26 9.14431773495871 4.0 4.0 4.0 4.0 33.0 27 9.343295320487613 4.0 4.0 4.0 4.0 35.0 28 9.183248132127408 4.0 4.0 4.0 4.0 32.0 29 9.217066456940621 4.0 4.0 4.0 4.0 32.0 30 9.22768383798663 4.0 4.0 4.0 4.0 33.0 31 9.473063311049941 4.0 4.0 4.0 4.0 35.0 32 9.28863546991742 4.0 4.0 4.0 4.0 35.0 33 9.408965788438852 4.0 4.0 4.0 4.0 35.0 34 9.337396775462052 4.0 4.0 4.0 4.0 35.0 35 9.164372788045615 4.0 4.0 4.0 4.0 35.0 36 8.643334644121117 4.0 4.0 4.0 4.0 33.0 37 8.419583169484861 4.0 4.0 4.0 4.0 32.0 38 8.47031065670468 4.0 4.0 4.0 4.0 32.0 39 8.500196618167518 4.0 4.0 4.0 4.0 32.0 40 8.386551317341722 4.0 4.0 4.0 4.0 31.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 16.921742340346988 1 2 -2.3016795865633073 1 3 -0.5907715023994076 1 4 -4.471031746031745 1 5 -1.5779808047249908 1 6 3.467054263565891 1 7 -5.805057216685121 1 8 -6.04814507198228 1 9 -5.1734403839055005 1 10 -3.8049095607235133 1 11 -4.119509043927652 1 12 -4.395441122185305 1 13 -3.941149870801035 1 14 -3.1840808416389805 1 15 -3.4230897009966776 1 16 -3.6430324843115534 1 17 -3.1138981173864897 1 18 -3.1637227759320767 1 19 -2.8961148025101533 1 20 -3.546899224806201 1 21 -4.0041159099298635 1 22 -3.3234957548911046 1 23 -3.5770948689553332 1 24 -3.105038759689921 1 25 -3.504143595422664 1 26 -4.251882613510519 1 27 -4.296142488002953 1 28 -4.14138981173865 1 29 -4.140974529346623 1 30 -4.301273532668882 1 31 -4.991232927279439 1 32 -4.953737541528241 1 33 -4.912246216315985 1 34 -4.9711609449981555 1 35 -4.973652639350314 1 36 -3.9466685123661867 1 37 -3.9069675156884465 1 38 -3.9313584348468087 1 39 -3.9543835363602824 1 40 -3.9427833148763396 2 1 -3.3004798818752334 2 2 1.4760981912144704 2 3 24.29811738648948 2 4 13.9734126984127 2 5 21.42201919527501 2 6 -2.7551679586563305 2 7 -5.805057216685121 2 8 -6.04814507198228 2 9 -5.1734403839055005 2 10 -3.8049095607235133 2 11 -4.119509043927652 2 12 -4.395441122185305 2 13 -3.941149870801035 2 14 -3.1840808416389805 2 15 -3.4230897009966776 2 16 -3.6430324843115534 2 17 -3.1138981173864897 2 18 -3.1637227759320767 2 19 -2.8961148025101533 2 20 -3.546899224806201 2 21 -4.0041159099298635 2 22 -3.3234957548911046 2 23 -3.5770948689553332 2 24 -3.105038759689921 2 25 -3.504143595422664 2 26 -4.251882613510519 2 27 -4.296142488002953 2 28 -4.14138981173865 2 29 -4.140974529346623 2 30 -4.301273532668882 2 31 -4.991232927279439 2 32 -4.953737541528241 2 33 -4.912246216315985 2 34 -4.9711609449981555 2 35 -4.973652639350314 2 36 -3.9466685123661867 2 37 -3.9069675156884465 2 38 -3.9313584348468087 2 39 -3.9543835363602824 2 40 -3.9427833148763396 6 1 21.699520118124767 6 2 2.4760981912144704 6 3 27.29811738648948 6 4 20.9734126984127 6 5 -6.577980804724991 6 6 -2.7551679586563305 6 7 -5.805057216685121 6 8 -6.04814507198228 6 9 -5.1734403839055005 6 10 -3.8049095607235133 6 11 -4.119509043927652 6 12 -4.395441122185305 6 13 -3.941149870801035 6 14 -3.1840808416389805 6 15 -3.4230897009966776 6 16 -3.6430324843115534 6 17 -3.1138981173864897 6 18 -3.1637227759320767 6 19 -2.8961148025101533 6 20 -3.546899224806201 6 21 -4.0041159099298635 6 22 -3.3234957548911046 6 23 -3.5770948689553332 6 24 -3.105038759689921 6 25 -3.504143595422664 6 26 -4.251882613510519 6 27 -4.296142488002953 6 28 -4.14138981173865 6 29 -4.140974529346623 6 30 -4.301273532668882 6 31 -4.991232927279439 6 32 -4.953737541528241 6 33 -4.912246216315985 6 34 -4.9711609449981555 6 35 -4.973652639350314 6 36 -3.9466685123661867 6 37 -3.9069675156884465 6 38 -3.9313584348468087 6 39 -3.9543835363602824 6 40 -3.9427833148763396 8 1 21.699520118124767 8 2 1.4760981912144704 8 3 21.29811738648948 8 4 23.9734126984127 8 5 22.42201919527501 8 6 -2.7551679586563305 8 7 -5.805057216685121 8 8 -6.04814507198228 8 9 -5.1734403839055005 8 10 -3.8049095607235133 8 11 -4.119509043927652 8 12 -4.395441122185305 8 13 -3.941149870801035 8 14 -3.1840808416389805 8 15 -3.4230897009966776 8 16 -3.6430324843115534 8 17 -3.1138981173864897 8 18 -3.1637227759320767 8 19 -2.8961148025101533 8 20 -3.546899224806201 8 21 -4.0041159099298635 8 22 -3.3234957548911046 8 23 -3.5770948689553332 8 24 -3.105038759689921 8 25 -3.504143595422664 8 26 -4.251882613510519 8 27 -4.296142488002953 8 28 -4.14138981173865 8 29 -4.140974529346623 8 30 -4.301273532668882 8 31 -4.991232927279439 8 32 -4.953737541528241 8 33 -4.912246216315985 8 34 -4.9711609449981555 8 35 -4.973652639350314 8 36 -3.9466685123661867 8 37 -3.9069675156884465 8 38 -3.9313584348468087 8 39 -3.9543835363602824 8 40 -3.9427833148763396 9 1 -4.8004798818752334 9 2 -1.5239018087855296 9 3 -1.9518826135105183 9 4 -5.0265873015873 9 5 -2.577980804724991 9 6 -0.0051679586563304625 9 7 -1.305057216685121 9 8 -2.2981450719822796 9 9 -0.42344038390550054 9 10 -3.0549095607235133 9 11 -2.3695090439276516 9 12 -0.6454411221853054 9 13 1.058850129198965 9 14 1.3159191583610195 9 15 1.3269102990033224 9 16 4.106967515688447 9 17 4.63610188261351 9 18 4.586277224067923 9 19 4.853885197489847 9 20 4.203100775193799 9 21 -4.0041159099298635 9 22 -3.3234957548911046 9 23 -3.5770948689553332 9 24 -3.105038759689921 9 25 -3.504143595422664 9 26 -4.251882613510519 9 27 -4.296142488002953 9 28 -4.14138981173865 9 29 -4.140974529346623 9 30 -4.301273532668882 9 31 -4.991232927279439 9 32 -4.953737541528241 9 33 -4.912246216315985 9 34 -4.9711609449981555 9 35 -4.973652639350314 9 36 -3.9466685123661867 9 37 -3.9069675156884465 9 38 -3.9313584348468087 9 39 -3.9543835363602824 9 40 -3.9427833148763396 10 1 -4.0504798818752334 10 2 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12 2 5.6427648578811365 12 3 0.13145071982281564 12 4 3.640079365079366 12 5 2.5886858619416753 12 6 2.4114987080103356 12 7 5.194942783314879 12 8 4.95185492801772 12 9 3.6598929494278334 12 10 7.528423772609821 12 11 2.8804909560723484 12 12 1.7712255444813607 12 13 7.558850129198965 12 14 5.8159191583610195 12 15 7.410243632336656 12 16 2.8569675156884466 12 17 3.8861018826135103 12 18 1.6696105574012572 12 19 3.6038851974898467 12 20 1.619767441860465 12 21 6.9958840900701365 12 22 7.676504245108895 12 23 6.089571797711333 12 24 6.728294573643413 12 25 6.82918973791067 12 26 6.748117386489481 12 27 6.870524178663713 12 28 7.025276854928016 12 29 5.192358803986711 12 30 6.8653931339977845 12 31 6.175433739387227 12 32 6.046262458471759 12 33 6.254420450350681 12 34 6.0288390550018445 12 35 6.193014027316352 12 36 5.553331487633813 12 37 1.2596991509782196 12 38 1.2353082318198574 12 39 1.2122831303063837 12 40 1.2238833517903265 13 1 -3.7290513104466623 13 2 -0.23818752307124313 13 3 -2.4161683277962327 13 4 -2.5980158730158713 13 5 -1.149409376153562 13 6 -0.1837393872277593 13 7 -0.6622000738279787 13 8 -0.6195736434108507 13 9 -0.459154669619787 13 10 0.19509043927648673 13 11 -0.1195090439276516 13 12 2.175987449243266 13 13 0.7731358434846785 13 14 2.3873477297895906 13 15 -0.2802325581395344 13 16 1.6426818014027331 13 17 3.0289590254706527 13 18 -0.4494370616463623 13 19 -0.6104005167958677 13 20 -1.546899224806201 13 21 -0.4326873385012924 13 22 -0.3234957548911046 13 23 -1.43423772609819 13 24 -0.9621816168327779 13 25 -1.3612864525655208 13 26 0.17668881506090983 13 27 0.2752860834256179 13 28 -0.2842469545957931 13 29 0.4304540420819478 13 30 -0.4441306755260248 13 31 -0.5626614987080103 13 32 -0.38230897009967 13 33 -0.4836747877445564 13 34 -0.5425895164267267 13 35 -0.9736526393503144 13 36 -1.5180970837947578 13 37 -1.7641103728313032 13 38 -0.9313584348468087 13 39 -0.6686692506459968 13 40 -1.5142118863049108 14 1 13.032853451458099 14 2 14.809431524547803 14 3 3.6314507198228156 14 4 10.306746031746032 14 5 7.755352528608341 14 6 3.9114987080103356 14 7 14.861609449981547 14 8 14.618521594684388 14 9 13.8265596160945 14 10 11.195090439276488 14 11 18.21382428940568 14 12 17.27122554448136 14 13 17.392183462532298 14 14 12.482585825027687 14 15 13.910243632336655 14 16 18.356967515688446 14 17 18.552768549280177 14 18 22.836277224067924 14 19 14.770551864156515 14 20 16.786434108527132 14 21 20.995884090070135 14 22 19.343170911775562 14 23 18.422905131044665 14 24 12.22829457364341 14 25 9.829189737910667 14 26 1.748117386489481 14 27 2.0371908453303806 14 28 -1.1413898117386498 14 29 1.1923588039867106 14 30 0.6987264673311184 14 31 5.342100406053895 14 32 5.379595791805093 14 33 5.421087117017349 14 34 5.362172388335178 14 35 5.3596806939830195 14 36 6.386664820967147 14 37 6.4263658176448875 14 38 6.401974898486525 14 39 6.3789497969730515 14 40 6.390550018456994 15 1 2.6995201181247666 15 2 5.47609819121447 15 3 -1.2018826135105183 15 4 1.4734126984126998 15 5 4.922019195275009 15 6 0.7448320413436695 15 7 0.6949427833148789 15 8 3.4518549280177204 15 9 4.3265596160944995 15 10 4.195090439276487 15 11 6.880490956072348 15 12 3.6045588778146946 15 13 -2.441149870801035 15 14 4.8159191583610195 15 15 4.076910299003322 15 16 9.856967515688446 15 17 10.88610188261351 15 18 -3.1637227759320767 15 19 -2.8961148025101533 15 20 -3.546899224806201 15 21 -4.0041159099298635 15 22 -3.3234957548911046 15 23 -3.5770948689553332 15 24 -3.105038759689921 15 25 -3.504143595422664 15 26 -4.251882613510519 15 27 -4.296142488002953 15 28 -4.14138981173865 15 29 -4.140974529346623 15 30 -4.301273532668882 15 31 -4.991232927279439 15 32 -4.953737541528241 15 33 -4.912246216315985 15 34 -4.9711609449981555 15 35 -4.973652639350314 15 36 -3.9466685123661867 15 37 -3.9069675156884465 15 38 -3.9313584348468087 15 39 -3.9543835363602824 15 40 -3.9427833148763396 16 1 -1.9671465485418995 16 2 -4.190568475452197 16 3 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3.964784053156148 17 4 4.640079365079368 17 5 10.42201919527501 17 6 0.24483204134366954 17 7 0.1949427833148789 17 8 2.2851882613510544 17 9 1.1598929494278334 17 10 -0.8049095607235133 17 11 3.5471576227390145 17 12 0.6045588778146946 17 13 3.725516795865631 17 14 0.48258582502768643 17 15 4.243576965669988 17 16 4.023634182355113 17 17 3.2194352159468442 17 18 2.169610557401257 17 19 0.7705518641565137 17 20 1.786434108527133 17 21 6.6625507567368025 17 22 7.009837578442229 17 23 7.089571797711333 17 24 7.228294573643413 17 25 7.162523071244002 17 26 6.081450719822815 17 27 6.037190845330381 17 28 6.191943521594684 17 29 6.192358803986711 17 30 6.032059800664452 17 31 5.342100406053895 17 32 5.379595791805093 17 33 5.421087117017349 17 34 5.362172388335178 17 35 -4.973652639350314 17 36 -3.9466685123661867 17 37 -3.9069675156884465 17 38 -3.9313584348468087 17 39 -3.9543835363602824 17 40 -3.9427833148763396 18 1 -4.0504798818752334 18 2 -3.0239018087855296 18 3 -2.9518826135105183 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108 32 -4.953737541528241 108 33 -4.912246216315985 108 34 -4.9711609449981555 108 35 -4.973652639350314 108 36 -3.9466685123661867 108 37 -3.9069675156884465 108 38 -3.9313584348468087 108 39 -3.9543835363602824 108 40 -3.9427833148763396 109 1 0.6995201181247666 109 2 7.47609819121447 109 3 -0.20188261351051828 109 4 4.9734126984127 109 5 6.422019195275009 109 6 1.7448320413436695 109 7 3.194942783314879 109 8 4.95185492801772 109 9 6.3265596160944995 109 10 2.6950904392764867 109 11 3.3804909560723484 109 12 1.6045588778146946 109 13 4.058850129198965 109 14 2.3159191583610195 109 15 1.5769102990033224 109 16 0.35696751568844665 109 17 4.88610188261351 109 18 -0.16372277593207674 109 19 -0.3961148025101533 109 20 4.453100775193799 109 21 4.4958840900701365 109 22 -0.3234957548911046 109 23 4.422905131044667 109 24 0.39496124031007884 109 25 2.995856404577336 109 26 1.748117386489481 109 27 -1.2961424880029533 109 28 3.85861018826135 109 29 6.359025470653377 109 30 7.198726467331118 109 31 8.00876707272056 109 32 9.546262458471759 109 33 8.087753783684015 109 34 8.028839055001844 109 35 8.026347360649686 109 36 -3.9466685123661867 109 37 -3.9069675156884465 109 38 -3.9313584348468087 109 39 -3.9543835363602824 109 40 -3.9427833148763396 110 1 -6.133813215208567 110 2 -4.690568475452197 110 3 -3.5352159468438513 110 4 -5.5265873015873 110 5 -4.411314138058324 110 6 -2.0885012919896635 110 7 -1.638390550018455 110 8 -3.0481450719822796 110 9 -2.0067737172388336 110 10 -2.6382428940568463 110 11 -2.2861757105943186 110 12 -2.5621077888519723 110 13 -2.107816537467702 110 14 0.6492524916943525 110 15 -0.2564230343300107 110 16 1.5236341823551127 110 17 0.05276854928017727 110 18 0.5029438907345902 110 19 -1.0627814691768203 110 20 0.7864341085271329 110 21 0.8292174234034704 110 22 1.5098375784422293 110 23 -3.5770948689553332 110 24 -3.105038759689921 110 25 -3.504143595422664 110 26 -4.251882613510519 110 27 -4.296142488002953 110 28 -4.14138981173865 110 29 -4.140974529346623 110 30 -4.301273532668882 110 31 -4.991232927279439 110 32 -4.953737541528241 110 33 -4.912246216315985 110 34 -4.9711609449981555 110 35 -4.973652639350314 110 36 -3.9466685123661867 110 37 -3.9069675156884465 110 38 -3.9313584348468087 110 39 -3.9543835363602824 110 40 -3.9427833148763396 112 1 15.699520118124767 112 2 -5.52390180878553 112 3 -4.701882613510518 112 4 -8.0265873015873 112 5 -6.577980804724991 112 6 -2.7551679586563305 112 7 -5.805057216685121 112 8 -6.04814507198228 112 9 -5.1734403839055005 112 10 -3.8049095607235133 112 11 -4.119509043927652 112 12 -4.395441122185305 112 13 -3.941149870801035 112 14 -3.1840808416389805 112 15 -3.4230897009966776 112 16 -3.6430324843115534 112 17 -3.1138981173864897 112 18 -3.1637227759320767 112 19 -2.8961148025101533 112 20 -3.546899224806201 112 21 -4.0041159099298635 112 22 -3.3234957548911046 112 23 -3.5770948689553332 112 24 -3.105038759689921 112 25 -3.504143595422664 112 26 -4.251882613510519 112 27 -4.296142488002953 112 28 -4.14138981173865 112 29 -4.140974529346623 112 30 -4.301273532668882 112 31 -4.991232927279439 112 32 -4.953737541528241 112 33 -4.912246216315985 112 34 -4.9711609449981555 112 35 -4.973652639350314 112 36 -3.9466685123661867 112 37 -3.9069675156884465 112 38 -3.9313584348468087 112 39 -3.9543835363602824 112 40 -3.9427833148763396 113 1 -10.300479881875233 113 2 -5.52390180878553 113 3 -4.701882613510518 113 4 -8.0265873015873 113 5 -6.577980804724991 113 6 -2.7551679586563305 113 7 -5.805057216685121 113 8 -6.04814507198228 113 9 -5.1734403839055005 113 10 -3.8049095607235133 113 11 -4.119509043927652 113 12 -4.395441122185305 113 13 -3.941149870801035 113 14 -3.1840808416389805 113 15 -3.4230897009966776 113 16 -3.6430324843115534 113 17 -3.1138981173864897 113 18 -3.1637227759320767 113 19 -2.8961148025101533 113 20 -3.546899224806201 113 21 -4.0041159099298635 113 22 -3.3234957548911046 113 23 -3.5770948689553332 113 24 -3.105038759689921 113 25 -3.504143595422664 113 26 -4.251882613510519 113 27 -4.296142488002953 113 28 -4.14138981173865 113 29 -4.140974529346623 113 30 -4.301273532668882 113 31 -4.991232927279439 113 32 -4.953737541528241 113 33 -4.912246216315985 113 34 -4.9711609449981555 113 35 -4.973652639350314 113 36 -3.9466685123661867 113 37 -3.9069675156884465 113 38 -3.9313584348468087 113 39 -3.9543835363602824 113 40 -3.9427833148763396 117 1 18.699520118124767 117 2 2.4760981912144704 117 3 17.29811738648948 117 4 19.9734126984127 117 5 -6.577980804724991 117 6 -2.7551679586563305 117 7 -5.805057216685121 117 8 -6.04814507198228 117 9 -5.1734403839055005 117 10 -3.8049095607235133 117 11 -4.119509043927652 117 12 -4.395441122185305 117 13 -3.941149870801035 117 14 -3.1840808416389805 117 15 -3.4230897009966776 117 16 -3.6430324843115534 117 17 -3.1138981173864897 117 18 -3.1637227759320767 117 19 -2.8961148025101533 117 20 -3.546899224806201 117 21 -4.0041159099298635 117 22 -3.3234957548911046 117 23 -3.5770948689553332 117 24 -3.105038759689921 117 25 -3.504143595422664 117 26 -4.251882613510519 117 27 -4.296142488002953 117 28 -4.14138981173865 117 29 -4.140974529346623 117 30 -4.301273532668882 117 31 -4.991232927279439 117 32 -4.953737541528241 117 33 -4.912246216315985 117 34 -4.9711609449981555 117 35 -4.973652639350314 117 36 -3.9466685123661867 117 37 -3.9069675156884465 117 38 -3.9313584348468087 117 39 -3.9543835363602824 117 40 -3.9427833148763396 118 1 -10.300479881875233 118 2 -5.52390180878553 118 3 -4.701882613510518 118 4 -8.0265873015873 118 5 -6.577980804724991 118 6 -2.7551679586563305 118 7 -5.805057216685121 118 8 -6.04814507198228 118 9 -5.1734403839055005 118 10 -3.8049095607235133 118 11 -4.119509043927652 118 12 -4.395441122185305 118 13 -3.941149870801035 118 14 -3.1840808416389805 118 15 -3.4230897009966776 118 16 -3.6430324843115534 118 17 -3.1138981173864897 118 18 -3.1637227759320767 118 19 -2.8961148025101533 118 20 -3.546899224806201 118 21 -4.0041159099298635 118 22 -3.3234957548911046 118 23 -3.5770948689553332 118 24 -3.105038759689921 118 25 -3.504143595422664 118 26 -4.251882613510519 118 27 -4.296142488002953 118 28 -4.14138981173865 118 29 -4.140974529346623 118 30 -4.301273532668882 118 31 -4.991232927279439 118 32 -4.953737541528241 118 33 -4.912246216315985 118 34 -4.9711609449981555 118 35 -4.973652639350314 118 36 -3.9466685123661867 118 37 -3.9069675156884465 118 38 -3.9313584348468087 118 39 -3.9543835363602824 118 40 -3.9427833148763396 119 1 10.699520118124767 119 2 15.47609819121447 119 3 20.29811738648948 119 4 24.9734126984127 119 5 26.42201919527501 119 6 20.24483204134367 119 7 19.19494278331488 119 8 19.95185492801772 119 9 25.8265596160945 119 10 -3.8049095607235133 119 11 -4.119509043927652 119 12 -4.395441122185305 119 13 -3.941149870801035 119 14 -3.1840808416389805 119 15 -3.4230897009966776 119 16 -3.6430324843115534 119 17 -3.1138981173864897 119 18 -3.1637227759320767 119 19 -2.8961148025101533 119 20 -3.546899224806201 119 21 -4.0041159099298635 119 22 -3.3234957548911046 119 23 -3.5770948689553332 119 24 -3.105038759689921 119 25 -3.504143595422664 119 26 -4.251882613510519 119 27 -4.296142488002953 119 28 -4.14138981173865 119 29 -4.140974529346623 119 30 -4.301273532668882 119 31 -4.991232927279439 119 32 -4.953737541528241 119 33 -4.912246216315985 119 34 -4.9711609449981555 119 35 -4.973652639350314 119 36 -3.9466685123661867 119 37 -3.9069675156884465 119 38 -3.9313584348468087 119 39 -3.9543835363602824 119 40 -3.9427833148763396 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 1700.0 5 36.0 6 64.0 7 33.0 8 23.0 9 21.0 10 25.0 11 18.0 12 24.0 13 32.0 14 14.0 15 18.0 16 12.0 17 22.0 18 29.0 19 25.0 20 43.0 21 71.0 22 62.0 23 62.0 24 69.0 25 45.0 26 40.0 27 25.0 28 18.0 29 9.0 30 2.0 31 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 0.0 100.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 0.0 100.0 7 97.10087370929308 0.5559968228752978 0.7148530579825259 1.6282764098490867 8 97.09005112072356 1.2976799056232795 0.8651199370821865 0.7471490365709791 9 92.13527329925284 2.791977978765238 2.320094376720409 2.752654345261502 10 38.81242626818718 27.290601651592606 13.173417223751475 20.723554856468738 11 29.57137239480928 22.886354699174206 20.88084939048368 26.661423515532835 12 34.8800629178136 13.605977192292567 24.498623672827367 27.015336217066455 13 23.830121903263862 16.122689736531655 30.672434132913885 29.3747542272906 14 19.23682140047207 21.79386309992132 32.926829268292686 26.042486231313926 15 27.054659850570197 12.976799056232796 30.86905230043256 29.09948879276445 16 27.015336217066455 16.791191506095164 31.773495871018483 24.4199764058199 17 24.184034604797482 16.044042469524182 31.537554069996066 28.234368855682263 18 21.745969327565867 20.290994887927642 29.689343295320487 28.273692489186004 19 19.968491532099254 18.196140212682156 27.688066167782594 34.147302087435996 20 21.15611482500983 20.055053086905232 29.532048761305546 29.256783326779395 21 20.684231222965003 18.20684231222965 32.48132127408573 28.627605190719624 22 16.201337003539127 21.07746755800236 31.655524970507276 31.06567046795124 23 20.055053086905232 16.90916240660637 31.773495871018483 31.262288635469915 24 19.77978765237908 20.369642154935118 32.91388124262682 26.936688950058986 25 17.9304897314376 14.454976303317535 30.134281200631914 37.480252764612956 26 14.808979913351713 15.320992516738874 31.547853485624263 38.32217408428515 27 16.21301775147929 15.384615384615385 35.26627218934911 33.13609467455622 28 16.436736302719748 18.052818289318093 34.686637761135195 30.82380764682696 29 18.71552403467297 16.312056737588655 36.485421591804574 28.486997635933808 30 17.191188040912667 14.083398898505115 41.46341463414634 27.26199842643588 31 17.03383162863887 15.066876475216365 39.77183320220299 28.127458693941776 32 18.58267716535433 13.818897637795274 40.19685039370079 27.401574803149607 33 16.33215269578906 11.255411255411255 43.722943722943725 28.689492325855966 34 12.033031852143138 13.802595359811246 49.35116004718836 24.813212740857253 35 11.010617381046009 15.80810066850177 49.311836413684624 23.869445536767596 36 12.549173878835562 17.466561762391816 45.672698662470495 24.31156569630212 37 13.591465823785065 15.448439352034768 46.819438956934015 24.140655867246146 38 14.353126228863546 16.71254423908769 45.14353126228863 23.790798269760124 39 14.51042076287849 14.589068029885963 44.63232402674007 26.268187180495477 40 11.167911915060952 13.802595359811246 45.497443963822256 29.532048761305546 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 2.0 10 4.0 11 5.0 12 6.0 13 6.0 14 14.0 15 22.0 16 25.0 17 28.0 18 28.0 19 35.0 20 42.0 21 53.0 22 64.0 23 64.0 24 59.5 25 55.0 26 76.5 27 98.0 28 98.0 29 84.0 30 70.0 31 64.5 32 59.0 33 59.0 34 68.5 35 78.0 36 77.0 37 76.0 38 76.0 39 94.0 40 112.0 41 114.5 42 117.0 43 117.0 44 125.5 45 134.0 46 144.0 47 154.0 48 154.0 49 155.0 50 156.0 51 160.5 52 165.0 53 165.0 54 166.0 55 167.0 56 169.0 57 171.0 58 171.0 59 175.0 60 179.0 61 170.0 62 161.0 63 161.0 64 150.0 65 139.0 66 115.5 67 92.0 68 92.0 69 83.5 70 75.0 71 62.0 72 49.0 73 49.0 74 39.0 75 29.0 76 23.5 77 18.0 78 18.0 79 16.5 80 15.0 81 8.5 82 2.0 83 2.0 84 2.0 85 2.0 86 1.5 87 1.0 88 1.0 89 2.0 90 3.0 91 1.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.9830908375933936 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.039323633503735744 15 0.0 16 0.0 17 0.0 18 0.0 19 0.15729453401494298 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.43255996854109324 26 0.15729453401494298 27 0.31458906802988595 28 0.23594180102241447 29 0.19661816751867872 30 0.039323633503735744 31 0.039323633503735744 32 0.11797090051120723 33 0.07864726700747149 34 0.0 35 0.0 36 0.039323633503735744 37 0.47188360204482893 38 0.0 39 0.0 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 2543.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.98898938261895 #Duplication Level Percentage of deduplicated Percentage of total 1 98.03359278984023 94.10145497443963 2 1.2290045063498567 2.359418010224145 3 0.3277345350266284 0.9437672040896579 4 0.12290045063498567 0.47188360204482893 5 0.0819336337566571 0.39323633503735744 6 0.04096681687832855 0.23594180102241447 7 0.04096681687832855 0.2752654345261502 8 0.0819336337566571 0.6291781360597719 9 0.0 0.0 >10 0.04096681687832855 0.5898545025560362 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TACTTCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT 15 0.5898545025560362 No Hit TACTTCGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.31458906802988595 No Hit TACTTCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT 8 0.31458906802988595 No Hit TACTTCGGGGGGCTGGTGAGATGGCCCAGTGGGTAAGAGC 7 0.2752654345261502 No Hit TACTTCGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.23594180102241447 No Hit TACTTCGGGGAGTCGCCTTTTTTTTTTTTTTTTTTTTTTT 5 0.19661816751867872 No Hit TACTTCGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.19661816751867872 No Hit TACTTCGGGGTCGCCTTTTTTTTTTTTTTTTTTTTTTTTT 4 0.15729453401494298 No Hit TACTTCGGGTTCGCTCGACTTTTTTTTTTTTTTTTTTTTT 4 0.15729453401494298 No Hit TACTTCGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4 0.15729453401494298 No Hit TACTTCGGGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3 0.11797090051120723 No Hit TACTTCGGGGCTTACTTCGGGAAGCATGGTATCAACGCAA 3 0.11797090051120723 No Hit TACTTCGGGGGGCCGGTGAGATGGCCCAGTGGGTAAGAGC 3 0.11797090051120723 No Hit TACTTCGGGGGGGCTGGTGAGATGGCTCCGTGGGTCCGCC 3 0.11797090051120723 No Hit TACTTCGGGGCGTCGACTTTTTTTTTTTTTTTTTTTTTTT 3 0.11797090051120723 No Hit TACTTCGGGGGGCTGGTGAGATGGCCCCGTGGGTAAGAGC 3 0.11797090051120723 No Hit TACTTCGGGTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3 0.11797090051120723 No Hit TACTTCGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTCT 3 0.11797090051120723 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.039323633503735744 0.0 0.0 0.0 0.0 15 0.039323633503735744 0.0 0.0 0.0 0.0 16 0.039323633503735744 0.0 0.0 0.0 0.0 17 0.039323633503735744 0.0 0.0 0.0 0.0 18 0.039323633503735744 0.0 0.0 0.0 0.0 19 0.039323633503735744 0.0 0.0 0.0 0.0 20 0.039323633503735744 0.0 0.0 0.0 0.0 21 0.039323633503735744 0.0 0.0 0.0 0.0 22 0.039323633503735744 0.0 0.0 0.0 0.0 23 0.039323633503735744 0.0 0.0 0.0 0.0 24 0.039323633503735744 0.0 0.0 0.0 0.0 25 0.039323633503735744 0.0 0.0 0.0 0.0 26 0.039323633503735744 0.0 0.0 0.0 0.0 27 0.039323633503735744 0.0 0.0 0.0 0.0 28 0.039323633503735744 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGGGG 25 1.3971509E-4 34.408165 5 TCGGGGA 25 1.3971509E-4 34.408165 5 TCGGGAT 25 1.3971509E-4 34.408165 5 TTCGGGT 35 5.606944E-7 34.40816 4 TCGGGTG 20 0.0021527105 34.40816 5 ACTTCGG 245 0.0 32.30154 2 TACTTCG 245 0.0 32.30154 1 CTTCGGG 235 0.0 31.47981 3 TTCGGGC 55 3.4742698E-10 31.28015 4 TTCGGGA 80 0.0 30.107143 4 TTCGGGG 70 3.6379788E-12 29.49271 4 TCGGGAA 30 4.0816318E-4 28.67347 5 CGGGAAG 25 0.0064282967 27.526531 6 >>END_MODULE