FastQCFastQC Report
Fri 17 Jun 2016
SRR1525999_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525999_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3003
Sequences flagged as poor quality0
Sequence length40
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTTCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG1304.329004329004329No Hit
TACTTCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT1163.862803862803863No Hit
TACTTCGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT270.8991008991008992No Hit
TACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.765900765900766No Hit
TACTTCGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG220.7326007326007326No Hit
TACTTCGGGTCACAGATCCTCTGAAGGAGATTGGGCGGAT170.5661005661005661No Hit
TACTTCGGGGCTTACTTCGGGTCACAGATCCTCTTCTGAG170.5661005661005661No Hit
TACTTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.4329004329004329No Hit
TACTTCGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGG100.333000333000333No Hit
TACTTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.2997002997002997No Hit
TACTTCGGGGAGTGCAGTGCTTACTTCGGGTCACAGATCC90.2997002997002997No Hit
TACTTCGGGGCTTACTTCGGGTCACAGATCCTCTTCTGAA90.2997002997002997No Hit
TACTTCGGGGTGCAGTGCTTACTTCGGGTCACAGATCCTC70.2331002331002331No Hit
TACTTCGGGTCACCGATCCTCTTCTGAGATGAGTTTTTGT70.2331002331002331No Hit
TACTTCGGGTCACAAATCCTCTTCTGAAGGAGATTGGGCG70.2331002331002331No Hit
TACTTCGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTT70.2331002331002331No Hit
TACTTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT70.2331002331002331No Hit
TACTTCGGGTCACCGATCCTCTTCTGAAGGAGATTGGGCG70.2331002331002331No Hit
TACTTCGGGTCACAGATACTCTTCTGAGATGAGTTTTTGT60.1998001998001998No Hit
TACTTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTT60.1998001998001998No Hit
TACTTCGGGTCACAGATCCCCTTCTGAAGGAGATTGGGCG60.1998001998001998No Hit
TACTTCGGGCTTACTTCGGGTCACAGATCCTCTTCTGAAG50.1665001665001665No Hit
TACTTCGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCG50.1665001665001665No Hit
TACTTCGGGTCCCAGATCCTCTTCTGAGATGAGTTTTTGT50.1665001665001665No Hit
TACTTCGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAA50.1665001665001665No Hit
TACTTCGGGCTTACTTCGGGTCACAGATCCTCTTCTGAGA50.1665001665001665No Hit
TACTTCGGGCAGAGTGCAGTGCTTACTTCGGGTCACAGAT50.1665001665001665No Hit
TACTTCGGGTCACAGATACTCTTCTGAAGGAGATTGGGCG50.1665001665001665No Hit
TACTTCGGGTCATAGATCCTCTTCTGAAGGAGATTGGGCG50.1665001665001665No Hit
TACTTCGGGTCACCGCTCCTCTTCTGAGATGAGTTTTTGT40.1332001332001332No Hit
TACTTCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGAG40.1332001332001332No Hit
TACTTCGGGTCACAGATCATCTTCTGAGATGAGTTTTTGT40.1332001332001332No Hit
TACTTCGGGTCCAAGATCCTCTTCTGAAGGAGATTGGGCG40.1332001332001332No Hit
TACTTCGGGTAACAGATCCTCTTCTGAGATGAGTTTTTGT40.1332001332001332No Hit
TACTTCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGC40.1332001332001332No Hit
TACTTCGGGATAGCAGGATTTGTTCACTGATTCCCATTAT40.1332001332001332No Hit
TACTTCGGGTCCCAGATCCTCTTCTGAAGGAGATTGGGCG40.1332001332001332No Hit
TACTTCGGGCAGATCCTCTTCTGAGATGAGTTTTTGTTCA40.1332001332001332No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGA200.002213715634.457636
CGGGCAG200.002213715634.457636
TCGGGAA403.9011866E-834.457635
TTCGGGC452.4429028E-934.4576264
TCGGGCA309.530793E-634.4576265
ATTGGGC200.00241101333.88333533
TTGGGCG200.00241101333.88333534
GGAGATT251.6199729E-433.8833329
TTCTGAA251.6199729E-433.8833322
GATTGGG251.6199729E-433.8833332
CTGAAGG251.6199729E-433.8833324
TGAAGGA251.6199729E-433.8833325
AAGGAGA251.6199729E-433.8833327
AGATTGG251.6199729E-433.8833331
GAGATTG251.6199729E-433.8833330
AGGAGAT251.6199729E-433.8833328
GAAGGAG251.6199729E-433.8833326
TTCGGGA800.032.3040284
ACTTCGG3300.030.2896482
TACTTCG3300.030.2896481
Produced by FastQC (version 0.11.5)