##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1525999_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3003 Sequences flagged as poor quality 0 Sequence length 40 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.096237096237097 30.0 4.0 33.0 4.0 34.0 2 12.231435231435231 4.0 4.0 20.0 4.0 30.0 3 11.177156177156178 4.0 4.0 18.0 4.0 28.0 4 12.09124209124209 4.0 4.0 20.0 4.0 30.0 5 12.145521145521146 4.0 4.0 21.0 4.0 30.0 6 12.173826173826173 4.0 4.0 22.0 4.0 31.0 7 12.567765567765568 4.0 4.0 24.0 4.0 32.0 8 12.475857475857476 4.0 4.0 24.0 4.0 31.0 9 12.031968031968033 4.0 4.0 23.0 4.0 31.0 10 11.019314019314018 4.0 4.0 19.0 4.0 31.0 11 11.013320013320014 4.0 4.0 19.0 4.0 31.0 12 10.973026973026974 4.0 4.0 19.0 4.0 31.0 13 10.025641025641026 4.0 4.0 14.0 4.0 29.0 14 10.421911421911421 4.0 4.0 15.0 4.0 31.0 15 10.04995004995005 4.0 4.0 14.0 4.0 30.0 16 8.946053946053945 4.0 4.0 9.0 4.0 27.0 17 8.557442557442558 4.0 4.0 8.0 4.0 24.0 18 8.127872127872127 4.0 4.0 7.0 4.0 22.0 19 8.015651015651015 4.0 4.0 4.0 4.0 22.0 20 8.835497835497835 4.0 4.0 4.0 4.0 27.0 21 7.937062937062937 4.0 4.0 4.0 4.0 23.0 22 8.024975024975024 4.0 4.0 4.0 4.0 24.0 23 8.15984015984016 4.0 4.0 4.0 4.0 24.0 24 7.755244755244755 4.0 4.0 4.0 4.0 22.0 25 8.061938061938061 4.0 4.0 4.0 4.0 24.0 26 8.181485181485181 4.0 4.0 4.0 4.0 25.0 27 8.435564435564435 4.0 4.0 4.0 4.0 27.0 28 8.598068598068599 4.0 4.0 4.0 4.0 27.0 29 7.781218781218781 4.0 4.0 4.0 4.0 23.0 30 8.106893106893107 4.0 4.0 4.0 4.0 25.0 31 8.977688977688977 4.0 4.0 4.0 4.0 30.0 32 8.878787878787879 4.0 4.0 4.0 4.0 30.0 33 8.692640692640692 4.0 4.0 4.0 4.0 29.0 34 8.680652680652681 4.0 4.0 4.0 4.0 29.0 35 8.63969363969364 4.0 4.0 4.0 4.0 30.0 36 8.525807525807526 4.0 4.0 4.0 4.0 31.0 37 8.367965367965368 4.0 4.0 4.0 4.0 30.0 38 8.335331335331336 4.0 4.0 4.0 4.0 31.0 39 8.134199134199134 4.0 4.0 4.0 4.0 30.0 40 8.154845154845155 4.0 4.0 4.0 4.0 30.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 13.141365280289332 1 2 11.123613622664255 1 3 14.948116335141652 1 4 23.72757685352622 1 5 18.92655213984328 1 6 5.892555756479808 1 7 7.828872814948767 1 8 5.331464737793851 1 9 6.577591922845086 1 10 2.681072935503316 1 11 7.673372513562388 1 12 8.434116937914407 1 13 8.290039180229053 1 14 -5.8992465340566635 1 15 -5.160262206148284 1 16 -4.580048221820375 1 17 -4.540717299578057 1 18 -3.7196805304400247 1 19 -3.9756781193490056 1 20 -4.50566606389391 1 21 -3.663954189270645 1 22 -3.9190174804098854 1 23 -4.005123568414708 1 24 -3.712296564195297 1 25 -3.8145117540687155 1 26 -4.056826401446653 1 27 -4.198372513562386 1 28 -4.607610006027727 1 29 -3.607670283303195 1 30 -4.3097197106690786 1 31 -5.194318866787221 1 32 -5.186060880048222 1 33 -4.898884870403858 1 34 -4.963652802893309 1 35 -4.872709463532249 1 36 -4.093550331525014 1 37 -4.16702833031947 1 38 -3.7434297769740814 1 39 -4.060051235684147 1 40 -4.186286919831222 4 1 3.4413652802893324 4 2 -4.076386377335745 4 3 8.548116335141653 4 4 1.2275768535262195 4 5 -2.1734478601567204 4 6 0.8925557564798083 4 7 -4.471127185051233 4 8 1.5314647377938506 4 9 1.0775919228450856 4 10 2.3810729355033153 4 11 1.4733725135623885 4 12 -2.6658830620855927 4 13 0.6900391802290535 4 14 -0.8992465340566635 4 15 3.2397377938517167 4 16 -1.580048221820375 4 17 3.659282700421942 4 18 7.080319469559976 4 19 1.0243218806509944 4 20 2.0943339361060893 4 21 3.136045810729356 4 22 0.8809825195901153 4 23 3.194876431585291 4 24 3.287703435804703 4 25 0.5854882459312849 4 26 3.3431735985533475 4 27 0.6016274864376143 4 28 2.7923899939722734 4 29 2.192329716696806 4 30 3.290280289330921 4 31 6.405681133212779 4 32 6.6139391199517785 4 33 0.7011151295961415 4 34 4.23634719710669 4 35 3.1272905364677506 4 36 7.5064496684749855 4 37 7.232971669680531 4 38 6.856570223025918 4 39 6.739948764315853 4 40 7.2137130801687785 5 1 4.891365280289332 5 2 1.123613622664255 5 3 11.198116335141652 5 4 15.22757685352622 5 5 14.67655213984328 5 6 7.392555756479808 5 7 2.3288728149487667 5 8 4.831464737793851 5 9 12.577591922845086 5 10 12.431072935503316 5 11 6.423372513562388 5 12 3.184116937914407 5 13 6.790039180229053 5 14 7.8507534659433365 5 15 -0.6602622061482837 5 16 1.919951778179625 5 17 2.9592827004219426 5 18 6.780319469559975 5 19 -1.9756781193490056 5 20 -2.5056660638939103 5 21 3.336045810729355 5 22 -1.4190174804098854 5 23 -1.2551235684147084 5 24 2.787703435804703 5 25 -1.3145117540687155 5 26 2.943173598553347 5 27 1.3016274864376136 5 28 1.642389993972273 5 29 -1.3576702833031948 5 30 2.1902802893309214 5 31 2.0556811332127793 5 32 -1.4360608800482222 5 33 2.851115129596142 5 34 2.786347197106691 5 35 -4.872709463532249 5 36 -4.093550331525014 5 37 -4.16702833031947 5 38 -3.7434297769740814 5 39 -4.060051235684147 5 40 -4.186286919831222 6 1 9.355650994575047 6 2 10.83789933694997 6 3 -3.6233122362869192 6 4 3.370433996383362 6 5 9.283694996986137 6 6 11.03541289933695 6 7 3.7574442435201956 6 8 11.617179023508136 6 9 11.934734779987943 6 10 2.8953586497890296 6 11 5.101943942133817 6 12 10.791259795057265 6 13 9.575753465943338 6 14 9.67218203737191 6 15 12.41116636528029 6 16 12.562808921036767 6 17 14.3164255575648 6 18 8.56603375527426 6 19 8.310036166365279 6 20 13.351476793248947 6 21 8.764617239300783 6 22 5.080982519590115 6 23 7.994876431585292 6 24 8.001989150090417 6 25 8.328345388788428 6 26 13.08603074141049 6 27 11.944484629294756 6 28 13.678104279686558 6 29 8.67804400241109 6 30 14.118851717902348 6 31 17.377109704641352 6 32 18.671081977094637 6 33 17.815400843881857 6 34 12.464918625678118 6 35 17.555861965039178 6 36 15.19216395418927 6 37 16.97582881253767 6 38 13.685141651597345 6 39 17.082805907172997 6 40 15.527998794454493 7 1 1.6413652802893317 7 2 0.12361362266425502 7 3 -4.480455093429777 7 4 -4.200994575045209 7 5 -5.144876431585292 7 6 -3.678872814948763 7 7 -6.8139843279083765 7 8 -5.382820976491863 7 9 -3.2081223628692 7 10 -3.5332127787823984 7 11 -4.898056057866183 7 12 -3.3515973477998786 7 13 -4.709960819770947 7 14 -3.3278179626280924 7 15 -2.1602622061482837 7 16 -1.8657625075346607 7 17 -2.6835744424352006 7 18 -2.7196805304400247 7 19 -2.118535262206149 7 20 -0.9342374924653392 7 21 -0.6639541892706449 7 22 -0.7761603375527422 7 23 -0.8622664255575652 7 24 0.1448462929475598 7 25 0.3283453887884269 7 26 -2.3425406871609384 7 27 -1.3412296564195296 7 28 -2.3218957203134414 7 29 -2.4648131404460516 7 30 -2.024005424954793 7 31 -3.0514617239300774 7 32 -1.4717751657625078 7 33 -3.327456298975287 7 34 -2.106509945750452 7 35 -2.3012808921036783 7 36 0.3350210970464147 7 37 -0.02417118746232738 7 38 0.6851416515973474 7 39 -0.9171940928270041 7 40 -0.1862869198312218 9 1 9.641365280289332 9 2 -4.876386377335745 9 3 7.948116335141652 9 4 2.2275768535262195 9 5 16.42655213984328 9 6 19.89255575647981 9 7 19.328872814948767 9 8 -7.668535262206149 9 9 -6.922408077154914 9 10 -5.818927064496684 9 11 -6.326627486437612 9 12 -6.065883062085593 9 13 -5.709960819770947 9 14 -5.8992465340566635 9 15 -5.160262206148284 9 16 -4.580048221820375 9 17 -4.540717299578057 9 18 -3.7196805304400247 9 19 -3.9756781193490056 9 20 -4.50566606389391 9 21 -3.663954189270645 9 22 -3.9190174804098854 9 23 -4.005123568414708 9 24 -3.712296564195297 9 25 -3.8145117540687155 9 26 -4.056826401446653 9 27 -4.198372513562386 9 28 -4.607610006027727 9 29 -3.607670283303195 9 30 -4.3097197106690786 9 31 -5.194318866787221 9 32 -5.186060880048222 9 33 -4.898884870403858 9 34 -4.963652802893309 9 35 -4.872709463532249 9 36 -4.093550331525014 9 37 -4.16702833031947 9 38 -3.7434297769740814 9 39 -4.060051235684147 9 40 -4.186286919831222 10 1 0.9270795660036164 10 2 -1.0192435201928873 10 3 2.519544906570223 10 4 2.941862567811933 10 5 -1.2877335744424343 10 6 7.749698613622666 10 7 6.471729957805909 10 8 6.188607594936709 10 9 9.649020494273659 10 10 8.752501506931889 10 11 6.5305153707052455 10 12 8.934116937914407 10 13 5.004324894514767 10 14 7.672182037371909 10 15 5.268309222423145 10 16 5.277094635322483 10 17 0.8878541289933715 10 18 4.280319469559975 10 19 3.310036166365281 10 20 7.637191078963232 10 21 2.9074743821579263 10 22 2.6524110910186858 10 23 7.852019288728149 10 24 3.1448462929475607 10 25 7.328345388788427 10 26 3.800316455696205 10 27 8.087341772151898 10 28 5.6781042796865595 10 29 1.5351868595539475 10 30 4.11885171790235 10 31 2.234252561784208 10 32 4.242510548523207 10 33 1.3868294153104284 10 34 5.322061482820978 10 35 6.841576250753464 10 36 5.335021097046415 10 37 5.261543098251959 10 38 5.113713080168776 10 39 2.9399487643158526 10 40 2.9565702230259205 11 1 10.04136528028933 11 2 0.22361362266425466 11 3 10.548116335141653 11 4 13.127576853526218 11 5 12.42655213984328 11 6 7.292555756479807 11 7 14.328872814948767 11 8 0.931464737793851 11 9 0.977591922845086 11 10 5.481072935503317 11 11 3.173372513562388 11 12 4.434116937914407 11 13 0.49003918022905246 11 14 1.8007534659433357 11 15 2.5397377938517156 11 16 -0.3800482218203758 11 17 3.659282700421942 11 18 2.1803194695599757 11 19 1.624321880650994 11 20 2.7943339361060904 11 21 2.2360458107293555 11 22 2.280982519590114 11 23 4.094876431585291 11 24 3.0877034358047037 11 25 4.585488245931285 11 26 2.8431735985533475 11 27 5.601627486437614 11 28 5.292389993972273 11 29 1.7923297166968055 11 30 4.890280289330921 11 31 4.10568113321278 11 32 0.9139391199517775 11 33 4.2011151295961415 11 34 4.136347197106691 11 35 5.82729053646775 11 36 4.206449668474987 11 37 1.632971669680531 11 38 2.2565702230259186 11 39 4.039948764315852 11 40 2.7137130801687785 12 1 9.141365280289332 12 2 5.290280289330923 12 3 8.448116335141652 12 4 7.060910186859552 12 5 13.42655213984328 12 6 20.559222423146476 12 7 18.828872814948767 12 8 18.49813140446052 12 9 19.410925256178416 12 10 13.181072935503316 12 11 13.50670584689572 12 12 12.26745027124774 12 13 15.623372513562385 12 14 14.434086799276669 12 15 12.006404460518384 12 16 7.419951778179625 12 17 8.292616033755275 12 18 7.613652802893309 12 19 3.5243218806509944 12 20 10.99433393610609 12 21 10.836045810729356 12 22 5.747649186256781 12 23 8.16154309825196 12 24 9.621036769138035 12 25 7.0188215792646185 12 26 7.109840265220013 12 27 10.301627486437614 12 28 7.559056660638941 12 29 3.225663050030139 12 30 4.523613622664255 12 31 5.139014466546113 12 32 8.64727245328511 12 33 2.934448462929476 12 34 4.536347197106691 12 35 3.4606238698010845 12 36 2.073116335141652 12 37 5.499638336347196 12 38 5.923236889692585 12 39 10.439948764315853 12 40 7.647046413502112 13 1 5.308031946956 13 2 7.290280289330923 13 3 -0.385216998191682 13 4 2.7275768535262195 13 5 1.5932188065099453 13 6 11.72588908981314 13 7 11.328872814948767 13 8 8.164798071127183 13 9 12.077591922845086 13 10 6.347739602169984 13 11 11.173372513562388 13 12 13.26745027124774 13 13 12.790039180229053 13 14 11.434086799276669 13 15 13.506404460518384 13 16 18.25328511151296 13 17 10.292616033755275 13 18 15.780319469559975 13 19 13.357655213984327 13 20 18.16100060277276 13 21 14.169379144062688 13 22 13.914315852923448 13 23 15.828209764918624 13 24 12.95437010247137 13 25 11.518821579264618 13 26 11.77650693188668 13 27 15.134960819770946 13 28 14.55905666063894 13 29 11.392329716696805 13 30 14.023613622664254 13 31 11.472347799879447 13 32 12.14727245328511 13 33 11.434448462929474 13 34 11.869680530440023 13 35 14.293957203134418 13 36 11.406449668474986 13 37 12.999638336347198 13 38 11.256570223025918 13 39 10.10661543098252 13 40 10.313713080168778 15 1 10.641365280289332 15 2 11.123613622664255 15 3 -3.051883664858348 15 4 19.22757685352622 15 5 12.42655213984328 15 6 16.89255575647981 15 7 18.328872814948767 15 8 21.33146473779385 15 9 19.077591922845087 15 10 20.181072935503316 15 11 27.673372513562388 15 12 24.934116937914407 15 13 25.29003918022905 15 14 14.100753465943336 15 15 7.839737793851716 15 16 3.419951778179625 15 17 3.4592827004219426 15 18 4.280319469559975 15 19 -0.9756781193490056 15 20 13.49433393610609 15 21 7.336045810729355 15 22 16.080982519590115 15 23 1.9948764315852916 15 24 2.287703435804703 15 25 13.185488245931285 15 26 2.943173598553347 15 27 2.8016274864376136 15 28 15.392389993972273 15 29 3.392329716696805 15 30 11.690280289330921 15 31 18.80568113321278 15 32 20.813939119951776 15 33 21.101115129596142 15 34 11.036347197106691 15 35 20.12729053646775 15 36 27.906449668474984 15 37 26.832971669680532 15 38 27.256570223025918 15 39 26.93994876431585 15 40 26.813713080168778 16 1 6.641365280289332 16 2 7.123613622664255 16 3 -5.051883664858348 16 4 12.22757685352622 16 5 17.42655213984328 16 6 -3.1074442435201917 16 7 5.328872814948767 16 8 18.33146473779385 16 9 18.077591922845087 16 10 17.181072935503316 16 11 15.673372513562388 16 12 15.934116937914407 16 13 12.290039180229053 16 14 14.100753465943336 16 15 19.839737793851718 16 16 24.419951778179623 16 17 24.459282700421944 16 18 17.280319469559977 16 19 20.024321880650994 16 20 24.494333936106088 16 21 24.336045810729356 16 22 24.080982519590115 16 23 27.99487643158529 16 24 27.2877034358047 16 25 28.185488245931285 16 26 24.943173598553347 16 27 24.801627486437614 16 28 24.392389993972273 16 29 21.392329716696807 16 30 24.69028028933092 16 31 25.80568113321278 16 32 26.813939119951776 16 33 22.101115129596142 16 34 22.036347197106693 16 35 21.12729053646775 16 36 -4.093550331525014 16 37 -4.16702833031947 16 38 -3.7434297769740814 16 39 -4.060051235684147 16 40 -4.186286919831222 17 1 11.498508137432186 17 2 11.266470765521397 17 3 9.376687763713079 17 4 15.370433996383362 17 5 18.283694996986135 17 6 22.035412899336954 17 7 21.757444243520197 17 8 21.045750452079567 17 9 21.791877637130803 17 10 19.89535864978903 17 11 19.24480108499096 17 12 19.791259795057265 17 13 17.147182037371913 17 14 21.529324894514765 17 15 21.554023508137433 17 16 9.562808921036767 17 17 7.602139843279085 17 18 7.137462326702833 17 19 8.167179023508137 17 20 8.351476793248947 17 21 3.193188667872213 17 22 3.223839662447257 17 23 3.5663050030138628 17 24 3.5734177215189895 17 25 -0.10022603978300104 17 26 -0.05682640144665285 17 27 0.5159132007233271 17 28 0.6781042796865595 17 29 1.1066154309825187 17 30 -0.024005424954792076 17 31 0.662823990355637 17 32 0.9567962628089202 17 33 1.5296865581675707 17 34 0.0363471971066911 17 35 2.1272905364677506 17 36 2.7635925256178435 17 37 4.404400241109101 17 38 4.542284508740205 17 39 2.082805907172995 17 40 2.242284508740207 18 1 5.641365280289332 18 2 7.83789933694997 18 3 0.948116335141652 18 4 4.799005424954792 18 5 3.9979807112718504 18 6 1.3211271850512372 18 7 8.186015672091624 18 8 7.760036166365278 18 9 7.934734779987943 18 10 8.323930078360458 18 11 9.959086799276673 18 12 13.50554550934298 18 13 9.861467751657626 18 14 10.815039180229052 18 15 9.839737793851716 18 16 5.848523206751054 18 17 6.030711271850514 18 18 -0.1482519590114535 18 19 3.738607594936708 18 20 5.208619650391803 18 21 3.336045810729355 18 22 3.0809825195901146 18 23 7.280590717299578 18 24 5.716274864376132 18 25 1.6140596745027134 18 26 2.800316455696205 18 27 7.801627486437614 18 28 7.8209614225437 18 29 2.9637582881253763 18 30 5.975994575045208 18 31 5.948538276069922 18 32 2.813939119951778 18 33 2.9582579867389995 18 34 5.607775768535262 18 35 4.698719107896322 18 36 4.335021097046415 18 37 4.690114526823388 18 38 1.2565702230259186 18 39 0.9399487643158526 18 40 0.5279987944544917 19 1 -3.8586347197106683 19 2 -3.376386377335745 19 3 -2.051883664858348 19 4 1.2275768535262195 19 5 2.926552139843279 19 6 3.8925557564798083 19 7 6.828872814948767 19 8 7.831464737793851 19 9 7.577591922845086 19 10 7.181072935503316 19 11 9.673372513562388 19 12 7.434116937914407 19 13 9.290039180229053 19 14 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-3.7434297769740814 119 39 -4.060051235684147 119 40 -4.186286919831222 120 1 8.391365280289332 120 2 -5.126386377335745 120 3 4.948116335141652 120 4 4.7275768535262195 120 5 -3.073447860156721 120 6 -2.8574442435201917 120 7 -3.4211271850512333 120 8 -3.4185352622061487 120 9 -2.1724080771549144 120 10 -3.068927064496684 120 11 -0.3266274864376122 120 12 -1.065883062085593 120 13 -1.2099608197709468 120 14 -2.6492465340566635 120 15 1.5897377938517163 120 16 -4.580048221820375 120 17 -4.540717299578057 120 18 -3.7196805304400247 120 19 -3.9756781193490056 120 20 -4.50566606389391 120 21 -3.663954189270645 120 22 -3.9190174804098854 120 23 -4.005123568414708 120 24 -3.712296564195297 120 25 -3.8145117540687155 120 26 -4.056826401446653 120 27 -4.198372513562386 120 28 -4.607610006027727 120 29 -3.607670283303195 120 30 -4.3097197106690786 120 31 -5.194318866787221 120 32 -5.186060880048222 120 33 -4.898884870403858 120 34 -4.963652802893309 120 35 -4.872709463532249 120 36 -4.093550331525014 120 37 -4.16702833031947 120 38 -3.7434297769740814 120 39 -4.060051235684147 120 40 -4.186286919831222 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 1590.0 5 123.0 6 55.0 7 97.0 8 61.0 9 99.0 10 79.0 11 70.0 12 48.0 13 42.0 14 44.0 15 27.0 16 23.0 17 25.0 18 26.0 19 21.0 20 45.0 21 78.0 22 89.0 23 89.0 24 79.0 25 58.0 26 48.0 27 34.0 28 26.0 29 10.0 30 11.0 31 5.0 32 0.0 33 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 0.0 100.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 0.0 100.0 7 98.73248832555038 0.46697798532354906 0.20013342228152103 0.600400266844563 8 96.86771076307897 1.732755748083972 0.6997667444185272 0.6997667444185272 9 93.83744170552964 1.965356429047302 2.9313790806129245 1.2658227848101267 10 26.226226226226224 27.89456122789456 33.233233233233236 12.64597931264598 11 15.156562291805464 26.682211858760823 19.053964023984012 39.107261825449704 12 24.117255163224517 41.57228514323784 19.32045303131246 14.990006662225182 13 14.31901431901432 26.20712620712621 23.043623043623043 36.43023643023643 14 15.151515151515152 53.54645354645354 18.04861804861805 13.253413253413255 15 43.82284382284382 24.20912420912421 18.148518148518146 13.819513819513821 16 14.385614385614387 47.086247086247084 27.472527472527474 11.055611055611056 17 20.47952047952048 21.045621045621047 46.053946053946056 12.42091242091242 18 18.614718614718615 24.608724608724607 18.847818847818846 37.928737928737924 19 16.833333333333332 24.333333333333336 23.633333333333333 35.199999999999996 20 15.584415584415584 26.673326673326674 45.58774558774559 12.154512154512155 21 15.184815184815184 20.07992007992008 27.57242757242757 37.162837162837164 22 11.78821178821179 21.045621045621047 47.31934731934732 19.846819846819848 23 12.287712287712289 30.003330003330003 42.75724275724276 14.951714951714951 24 11.025982678214524 30.812791472351762 21.7854763491006 36.37574950033311 25 14.161946017994001 26.824391869376875 41.65278240586471 17.36087970676441 26 41.62775183455637 28.619079386257507 17.311541027351566 12.441627751834556 27 17.42904841402337 48.94824707846411 18.363939899833053 15.258764607679465 28 27.00900300100033 44.68156052017339 17.239079693231076 11.070356785595198 29 31.4647981314648 36.5699032365699 19.085752419085754 12.879546212879548 30 27.37579193064355 31.710570190063354 28.50950316772257 12.404134711570522 31 27.381745502998 39.20719520319787 20.686209193870752 12.724850099933377 32 28.414023372287144 36.86143572621035 23.30550918196995 11.419031719532555 33 26.606726606726603 41.02564102564102 17.74891774891775 14.618714618714618 34 14.219114219114218 27.03962703962704 45.887445887445885 12.853812853812855 35 15.184815184815184 29.03762903762904 44.68864468864469 11.08891108891109 36 25.658992325658993 26.89356022689356 31.197864531197865 16.249582916249583 37 25.47452547452547 27.405927405927404 34.66533466533466 12.454212454212454 38 28.63802863802864 27.539127539127538 30.802530802530804 13.02031302031302 39 22.677322677322678 32.06793206793207 20.546120546120548 24.708624708624708 40 24.775224775224775 27.372627372627374 35.231435231435235 12.62071262071262 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 1.0 6 1.5 7 2.0 8 2.0 9 2.5 10 3.0 11 20.5 12 38.0 13 38.0 14 46.5 15 55.0 16 57.5 17 60.0 18 60.0 19 47.0 20 34.0 21 40.5 22 47.0 23 47.0 24 45.0 25 43.0 26 54.5 27 66.0 28 66.0 29 72.0 30 78.0 31 89.5 32 101.0 33 101.0 34 108.0 35 115.0 36 141.0 37 167.0 38 167.0 39 204.0 40 241.0 41 283.5 42 326.0 43 326.0 44 276.5 45 227.0 46 228.0 47 229.0 48 229.0 49 275.0 50 321.0 51 370.0 52 419.0 53 419.0 54 316.0 55 213.0 56 161.0 57 109.0 58 109.0 59 86.0 60 63.0 61 45.5 62 28.0 63 28.0 64 17.5 65 7.0 66 7.0 67 7.0 68 7.0 69 5.0 70 3.0 71 1.5 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.1665001665001665 8 0.0666000666000666 9 0.0333000333000333 10 0.1998001998001998 11 0.0333000333000333 12 0.0333000333000333 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0999000999000999 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0333000333000333 25 0.0666000666000666 26 0.1665001665001665 27 0.2664002664002664 28 0.1332001332001332 29 0.1998001998001998 30 0.1332001332001332 31 0.0333000333000333 32 0.2664002664002664 33 0.0 34 0.0 35 0.0 36 0.1998001998001998 37 0.0 38 0.0 39 0.0 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 3003.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.35497835497836 #Duplication Level Percentage of deduplicated Percentage of total 1 93.03017424564386 72.89377289377289 2 4.3348916277093075 6.793206793206793 3 1.019974500637484 2.3976023976023977 4 0.3824904377390565 1.1988011988011988 5 0.3399915002124947 1.332001332001332 6 0.1274968125796855 0.5994005994005994 7 0.254993625159371 1.3986013986013985 8 0.0 0.0 9 0.1274968125796855 0.8991008991008992 >10 0.2974925626859329 4.295704295704296 >50 0.0 0.0 >100 0.08499787505312367 8.191808191808192 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TACTTCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG 130 4.329004329004329 No Hit TACTTCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT 116 3.862803862803863 No Hit TACTTCGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT 27 0.8991008991008992 No Hit TACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 23 0.765900765900766 No Hit TACTTCGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG 22 0.7326007326007326 No Hit TACTTCGGGTCACAGATCCTCTGAAGGAGATTGGGCGGAT 17 0.5661005661005661 No Hit TACTTCGGGGCTTACTTCGGGTCACAGATCCTCTTCTGAG 17 0.5661005661005661 No Hit TACTTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 13 0.4329004329004329 No Hit TACTTCGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGG 10 0.333000333000333 No Hit TACTTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9 0.2997002997002997 No Hit TACTTCGGGGAGTGCAGTGCTTACTTCGGGTCACAGATCC 9 0.2997002997002997 No Hit TACTTCGGGGCTTACTTCGGGTCACAGATCCTCTTCTGAA 9 0.2997002997002997 No Hit TACTTCGGGGTGCAGTGCTTACTTCGGGTCACAGATCCTC 7 0.2331002331002331 No Hit TACTTCGGGTCACCGATCCTCTTCTGAGATGAGTTTTTGT 7 0.2331002331002331 No Hit TACTTCGGGTCACAAATCCTCTTCTGAAGGAGATTGGGCG 7 0.2331002331002331 No Hit TACTTCGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTT 7 0.2331002331002331 No Hit TACTTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT 7 0.2331002331002331 No Hit TACTTCGGGTCACCGATCCTCTTCTGAAGGAGATTGGGCG 7 0.2331002331002331 No Hit TACTTCGGGTCACAGATACTCTTCTGAGATGAGTTTTTGT 6 0.1998001998001998 No Hit TACTTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTT 6 0.1998001998001998 No Hit TACTTCGGGTCACAGATCCCCTTCTGAAGGAGATTGGGCG 6 0.1998001998001998 No Hit TACTTCGGGCTTACTTCGGGTCACAGATCCTCTTCTGAAG 5 0.1665001665001665 No Hit TACTTCGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCG 5 0.1665001665001665 No Hit TACTTCGGGTCCCAGATCCTCTTCTGAGATGAGTTTTTGT 5 0.1665001665001665 No Hit TACTTCGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAA 5 0.1665001665001665 No Hit TACTTCGGGCTTACTTCGGGTCACAGATCCTCTTCTGAGA 5 0.1665001665001665 No Hit TACTTCGGGCAGAGTGCAGTGCTTACTTCGGGTCACAGAT 5 0.1665001665001665 No Hit TACTTCGGGTCACAGATACTCTTCTGAAGGAGATTGGGCG 5 0.1665001665001665 No Hit TACTTCGGGTCATAGATCCTCTTCTGAAGGAGATTGGGCG 5 0.1665001665001665 No Hit TACTTCGGGTCACCGCTCCTCTTCTGAGATGAGTTTTTGT 4 0.1332001332001332 No Hit TACTTCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGAG 4 0.1332001332001332 No Hit TACTTCGGGTCACAGATCATCTTCTGAGATGAGTTTTTGT 4 0.1332001332001332 No Hit TACTTCGGGTCCAAGATCCTCTTCTGAAGGAGATTGGGCG 4 0.1332001332001332 No Hit TACTTCGGGTAACAGATCCTCTTCTGAGATGAGTTTTTGT 4 0.1332001332001332 No Hit TACTTCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGC 4 0.1332001332001332 No Hit TACTTCGGGATAGCAGGATTTGTTCACTGATTCCCATTAT 4 0.1332001332001332 No Hit TACTTCGGGTCCCAGATCCTCTTCTGAAGGAGATTGGGCG 4 0.1332001332001332 No Hit TACTTCGGGCAGATCCTCTTCTGAGATGAGTTTTTGTTCA 4 0.1332001332001332 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.033300033300033303 0.0 0.0 0.0 0.0 14 0.06660006660006661 0.0 0.0 0.0 0.0 15 0.06660006660006661 0.0 0.0 0.0 0.0 16 0.06660006660006661 0.0 0.0 0.0 0.0 17 0.0999000999000999 0.0 0.0 0.0 0.0 18 0.0999000999000999 0.0 0.0 0.0 0.0 19 0.0999000999000999 0.0 0.0 0.0 0.0 20 0.0999000999000999 0.0 0.0 0.0 0.0 21 0.0999000999000999 0.0 0.0 0.0 0.0 22 0.13320013320013321 0.0 0.0 0.0 0.0 23 0.13320013320013321 0.0 0.0 0.0 0.0 24 0.1665001665001665 0.0 0.0 0.0 0.0 25 0.1665001665001665 0.0 0.0 0.0 0.0 26 0.1665001665001665 0.0 0.0 0.0 0.0 27 0.1665001665001665 0.0 0.0 0.0 0.0 28 0.1665001665001665 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGGGA 20 0.0022137156 34.45763 6 CGGGCAG 20 0.0022137156 34.45763 6 TCGGGAA 40 3.9011866E-8 34.45763 5 TTCGGGC 45 2.4429028E-9 34.457626 4 TCGGGCA 30 9.530793E-6 34.457626 5 ATTGGGC 20 0.002411013 33.883335 33 TTGGGCG 20 0.002411013 33.883335 34 GGAGATT 25 1.6199729E-4 33.88333 29 TTCTGAA 25 1.6199729E-4 33.88333 22 GATTGGG 25 1.6199729E-4 33.88333 32 CTGAAGG 25 1.6199729E-4 33.88333 24 TGAAGGA 25 1.6199729E-4 33.88333 25 AAGGAGA 25 1.6199729E-4 33.88333 27 AGATTGG 25 1.6199729E-4 33.88333 31 GAGATTG 25 1.6199729E-4 33.88333 30 AGGAGAT 25 1.6199729E-4 33.88333 28 GAAGGAG 25 1.6199729E-4 33.88333 26 TTCGGGA 80 0.0 32.304028 4 ACTTCGG 330 0.0 30.289648 2 TACTTCG 330 0.0 30.289648 1 >>END_MODULE