FastQCFastQC Report
Fri 17 Jun 2016
SRR1525998_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525998_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172352
Sequences flagged as poor quality0
Sequence length56
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG46432.6939054957296693No Hit
ACAATAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT13170.7641338655774229No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG12230.7095943186037875No Hit
ACAATAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC10860.6301058299294466No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG5490.31853416264389156No Hit
ACAATAGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT5380.31215187523208315No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG4970.28836334942443376No Hit
ACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3890.2257008911994059No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG3850.2233800594132937No Hit
ACAATAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC3710.21525714816190122No Hit
ACAATAGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC3190.18508633494244336No Hit
ACAATAGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT3020.17522279985146677No Hit
ACAATAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT3010.17464259190493875No Hit
ACAATAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC2990.17348217601188265No Hit
ACAATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC2970.17232176011882658No Hit
ACAATAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT2710.15723635350909765No Hit
ACAATAGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT2620.15201448199034534No Hit
ACAATAGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC2550.14795302636464908No Hit
ACAATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC2350.1363488674340884No Hit
ACAATAGGGATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT2040.11836242109171927No Hit
ACAATAGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA2020.1172020051986632No Hit
ACAATAGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC1990.1154613813590791No Hit
ACAATAGGGACTAATCCTAGCCCTAGCCCTACACAAATATCATTATACTATTATAT1860.10791867805421464No Hit
ACAATAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA1830.10617805421463053No Hit
ACAATAGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG1830.10617805421463053No Hit
ACAATAGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGG1800.10443743037504642No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC1770.1026968065354623No Hit
ACAATAGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC1740.10095618269587821No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGAC150.00799306550.66055741
TAGACCT203.9809992E-450.48143837
GGCGCAT700.050.48143437
CGTATCT150.008153127550.4071836
GCGTATC150.00819113350.3479335
TAAGAGA150.00823882550.27406334
ACTCGAA150.00824838950.25931546
TATACTA900.050.2004144
TAGTGGT204.127879E-450.11231615
AGATTAC402.6138878E-950.0976649
GATTACG402.6411726E-950.0391450
ATAATAG150.00851952749.84987316
CGCTGAT150.00851952749.84987328
TAGCTAT150.00851952749.84987331
TAATAGC150.00854896649.80639617
AGGGACC3000.049.7052546
TAGGGTA12550.049.3091935
AGGGTAC11150.049.2594646
GGGTACC10700.049.0084467
TAGGGAC9550.048.9245455
Produced by FastQC (version 0.11.5)