##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1525998_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 172352 Sequences flagged as poor quality 0 Sequence length 56 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.62562662458225 38.0 36.0 39.0 33.0 40.0 2 35.50469968436688 39.0 36.0 39.0 27.0 40.0 3 35.84203838655774 39.0 36.0 39.0 30.0 40.0 4 34.870184274043815 39.0 35.0 39.0 23.0 40.0 5 34.81259863535091 39.0 35.0 39.0 24.0 40.0 6 33.729576680282214 38.0 34.0 39.0 14.0 40.0 7 34.41269611028593 38.0 35.0 39.0 23.0 40.0 8 34.61582691236539 38.0 35.0 39.0 24.0 40.0 9 34.644199080950614 38.0 35.0 39.0 24.0 40.0 10 33.992724192350536 38.0 33.0 39.0 21.0 40.0 11 33.621408512810994 38.0 33.0 39.0 21.0 40.0 12 32.440122539918306 37.0 30.0 39.0 16.0 40.0 13 31.79704906238396 37.0 29.0 39.0 14.0 40.0 14 32.54067837913108 38.0 30.0 39.0 17.0 40.0 15 31.381631776828815 36.0 28.0 39.0 11.0 40.0 16 31.892087124025252 36.0 29.0 39.0 15.0 40.0 17 30.96616807463795 36.0 27.0 39.0 12.0 40.0 18 30.388315772372817 35.0 25.0 39.0 12.0 40.0 19 31.575931813962125 36.0 28.0 39.0 15.0 40.0 20 32.18297437801708 36.0 30.0 39.0 18.0 40.0 21 32.448123607500925 38.0 30.0 39.0 16.0 40.0 22 31.84315818789454 37.0 29.0 39.0 14.0 40.0 23 32.502436873375416 38.0 30.0 39.0 17.0 40.0 24 31.896926058299293 37.0 29.0 39.0 15.0 40.0 25 32.311536854808764 37.0 30.0 39.0 17.0 40.0 26 32.846389946156705 38.0 32.0 39.0 16.0 40.0 27 33.000243687337544 38.0 32.0 39.0 18.0 40.0 28 32.91115275714816 38.0 32.0 39.0 18.0 40.0 29 32.52455440029706 37.0 31.0 39.0 15.0 40.0 30 32.222324080950614 37.0 31.0 39.0 14.0 40.0 31 33.009706878945416 38.0 33.0 39.0 16.0 40.0 32 32.80610610842926 38.0 33.0 39.0 15.0 40.0 33 32.5989138507241 37.0 32.0 39.0 12.0 40.0 34 32.51011882658745 37.0 32.0 39.0 11.0 40.0 35 32.365884933160046 37.0 32.0 39.0 10.0 40.0 36 31.504531424062385 36.0 30.0 38.0 4.0 40.0 37 31.22578212031192 36.0 30.0 38.0 4.0 40.0 38 30.58257519494987 35.0 29.0 38.0 4.0 40.0 39 29.71439844040104 35.0 27.0 38.0 4.0 40.0 40 27.51304307463795 33.0 18.0 38.0 4.0 39.0 41 25.64288781099146 31.0 13.0 37.0 4.0 39.0 42 26.559343668770886 32.0 18.0 37.0 4.0 39.0 43 26.555659348310435 32.0 18.0 37.0 4.0 39.0 44 25.599824777200148 31.0 14.0 37.0 4.0 39.0 45 26.631103787597475 32.0 19.0 37.0 4.0 39.0 46 24.61835081693279 29.0 11.0 36.0 4.0 39.0 47 23.689890456739697 28.0 7.0 36.0 4.0 39.0 48 24.361655217229856 29.0 10.0 36.0 4.0 39.0 49 23.33741993130338 28.0 4.0 35.0 4.0 39.0 50 23.967601188265874 29.0 4.0 36.0 4.0 39.0 51 24.230151086149277 30.0 4.0 36.0 4.0 39.0 52 24.127750185666542 30.0 4.0 36.0 4.0 39.0 53 23.91650227441515 30.0 4.0 36.0 4.0 39.0 54 23.508146119569254 29.0 4.0 36.0 4.0 39.0 55 23.252291821388784 29.0 4.0 36.0 4.0 39.0 56 22.5817512996658 28.0 4.0 36.0 4.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 3.2741040696293666 1 2 4.918242140709793 1 3 4.476051565996592 1 4 5.835759210317235 1 5 5.790012442898522 1 6 7.308293736568665 1 7 6.150941168606224 1 8 5.871815640204012 1 9 5.804575891050838 1 10 6.503824986661215 1 11 2.2834828437833252 1 12 -4.441097214277008 1 13 -16.164172062104733 1 14 -5.423097859414295 1 15 3.2382301725340206 1 16 -0.5334724048462505 1 17 -4.574115898652138 1 18 -3.241372182429231 1 19 1.0080461625189763 1 20 0.43775589244702573 1 21 7.079562331992456 1 22 7.9040230319291815 1 23 6.82871572643926 1 24 7.4496900706362155 1 25 6.835923973103078 1 26 6.604253748798595 1 27 6.488157607067734 1 28 6.418528011086256 1 29 7.018465451162605 1 30 7.005853054690768 1 31 5.709969280290938 1 32 5.570317402334261 1 33 5.833093711624329 1 34 5.040356285545343 1 35 5.6493232019973725 1 36 4.415583408260389 1 37 4.650603259891891 1 38 4.73161481288777 1 39 4.629854305503219 1 40 -9.371406843479171 1 41 -0.08319162993083395 1 42 -1.577155761191289 1 43 -8.358727791589336 1 44 -14.362605075309263 1 45 -2.1258454430217277 1 46 -4.153641911343225 1 47 -9.261723954456457 1 48 -3.992131356912477 1 49 -12.788421812008966 1 50 -2.7824398371294983 1 51 3.233863422988268 1 52 2.8091657379395336 1 53 3.2935283119325973 1 54 3.7098978029241856 1 55 3.1961729538274426 1 56 6.194799805653055 2 1 3.833066954599097 2 2 5.4720194505281725 2 3 4.843490365680722 2 4 6.366135625163253 2 5 6.089459665851926 2 6 7.7818657291983016 2 7 6.518722163604906 2 8 6.320144147705989 2 9 6.403996791287746 2 10 6.671448045360876 2 11 5.790642622672507 2 12 2.3877524830117487 2 13 3.7681522337626063 2 14 6.9049358336628295 2 15 7.345047756108645 2 16 5.7832425201340065 2 17 7.803720374051203 2 18 5.822723632258846 2 19 6.995700808478436 2 20 6.393323146987694 2 21 7.6767984467594985 2 22 8.553983547854425 2 23 7.43774441820025 2 24 8.075302073794937 2 25 7.2867794613894645 2 26 6.908939192336373 2 27 6.584498749473632 2 28 6.7495624938448735 2 29 7.309252500386087 2 30 7.092507437423066 2 31 5.827368595900314 2 32 6.149575101728836 2 33 6.160021850608274 2 34 5.937276527714332 2 35 5.668644075911562 2 36 6.840616311656021 2 37 6.742364249626032 2 38 6.802028079536889 2 39 7.2496226655979825 2 40 6.607903501348414 2 41 10.399356409026787 2 42 9.01399980606325 2 43 8.507842358450144 2 44 -2.120725633350851 2 45 8.752254056846656 2 46 7.059914287656511 2 47 3.267625874445887 2 48 7.155881277991444 2 49 0.3142631050744775 2 50 7.600239815410642 2 51 10.268951604088556 2 52 9.972077030384916 2 53 10.237039762314279 2 54 9.840484799502232 2 55 9.808360371568952 2 56 10.218042764326391 3 1 3.624227712248441 3 2 5.35349103867776 3 3 4.7555107639323 3 4 6.000472681986949 3 5 6.020202745016569 3 6 7.630651547556731 3 7 6.470664854231423 3 8 6.22145546387889 3 9 6.116988534852581 3 10 6.690122159008276 3 11 6.6936537931436035 3 12 7.415047286314323 3 13 8.004116293986016 3 14 7.355178669991144 3 15 2.8827845215287518 3 16 7.44196034432051 3 17 8.586673263803512 3 18 9.254972296659037 3 19 7.503423003718847 3 20 7.165688372825482 3 21 7.857274405962997 3 22 8.83193400924345 3 23 7.957438444426568 3 24 8.415661471560846 3 25 8.21123307965076 3 26 6.856000872763762 3 27 6.37128845321331 3 28 6.3586688561566795 3 29 7.063890674256896 3 30 7.1784957812793095 3 31 5.875085934414155 3 32 5.674902930772063 3 33 6.198511408646155 3 34 5.896285755494809 3 35 5.703053983633762 3 36 7.183404072705073 3 37 7.417886347731912 3 38 7.695301513242569 3 39 8.345664433446451 3 40 10.188452311450405 3 41 12.694305413397842 3 42 11.73264963607782 3 43 11.553082766415177 3 44 11.339254939742883 3 45 11.445114668794208 3 46 12.765936628598716 3 47 13.254027040060262 3 48 12.256148397952593 3 49 13.187575392592688 3 50 12.703663807639881 3 51 12.099791817784528 3 52 11.861149395610756 3 53 11.915208776398782 3 54 11.998522681969451 3 55 11.810327345828298 3 56 11.282612944025274 4 1 3.3725959705789066 4 2 5.453444417487198 4 3 4.728531817080452 4 4 6.110302391954072 4 5 6.126621746832342 4 6 7.73318136058122 4 7 6.2318784774340585 4 8 5.9995385968228945 4 9 6.268961347337246 4 10 6.5204087566428655 4 11 6.81234152931674 4 12 7.925647472799088 4 13 8.445876611991608 4 14 7.6764845540761435 4 15 7.961279883947167 4 16 8.29731196624719 4 17 9.210268493719596 4 18 9.829271751927234 4 19 8.471646981733276 4 20 7.892193746531131 4 21 7.719054844692934 4 22 8.398823148959796 4 23 7.499753309225117 4 24 8.22628128802058 4 25 7.718042227219094 4 26 6.152781556868387 4 27 6.384931285818702 4 28 5.8923956069050725 4 29 6.400397520210667 4 30 6.784877976892005 4 31 5.364915153631902 4 32 5.538413261046394 4 33 5.647706579672317 4 34 5.373441372121398 4 35 5.905181382171325 4 36 6.280253461722104 4 37 6.376246263323022 4 38 6.982672078095099 4 39 6.981810578610116 4 40 3.163436362095741 4 41 11.017414801433368 4 42 10.345178467603564 4 43 10.380400330335288 4 44 11.392574859259984 4 45 10.077641537322446 4 46 12.663590846589585 4 47 13.147657113181918 4 48 12.8797068216656 4 49 12.763776501686277 4 50 13.222839839440436 4 51 12.507555472885869 4 52 12.080956040357282 4 53 11.98372965778466 4 54 12.213102845879742 4 55 11.987033926777414 4 56 11.269154142435248 5 1 3.660964377728682 5 2 5.43795994598441 5 3 4.827954451327429 5 4 6.446143094352841 5 5 6.223817937979405 5 6 7.495895356983688 5 7 6.321112304274642 5 8 6.123779153307879 5 9 6.258609015861381 5 10 6.5805604566642515 5 11 6.711170445403738 5 12 7.701694970251882 5 13 8.321208377171047 5 14 7.756498139152686 5 15 9.073281292769916 5 16 8.340394269169241 5 17 9.477403929385453 5 18 9.876190581094892 5 19 8.613938079734506 5 20 8.08921509271158 5 21 7.965196305591817 5 22 8.721368086631973 5 23 7.554898656965339 5 24 8.304307426714146 5 25 7.785024201835128 5 26 6.819620133454261 5 27 6.335899222522308 5 28 6.580982884732467 5 29 6.951926470260354 5 30 6.6944743240158715 5 31 5.577773177003262 5 32 5.883335838688879 5 33 6.042755573999287 5 34 5.583884270774213 5 35 6.28058233061093 5 36 7.514847607560419 5 37 7.682212376104314 5 38 7.8393117842549245 5 39 8.621214847846204 5 40 10.659279077098446 5 41 12.990181754476808 5 42 11.87636753916421 5 43 11.702203120307484 5 44 12.605055519394075 5 45 11.777549712268044 5 46 13.286529499905981 5 47 13.65558803055195 5 48 13.457437359156636 5 49 13.913702915855009 5 50 13.78907337696921 5 51 12.703645989747713 5 52 12.950690250850496 5 53 12.844620989751103 5 54 13.093478699664065 5 55 12.97879482943027 5 56 12.313947158699602 6 1 3.5551614629005 6 2 5.274956870706966 6 3 5.015265589180082 6 4 6.244551972545111 6 5 6.282435400206971 6 6 7.657855512084637 6 7 6.330471844320108 6 8 6.065482589596151 6 9 6.10386907736693 6 10 6.66161407345794 6 11 7.070336109262541 6 12 8.077279941504724 6 13 8.799186716521227 6 14 7.748997136344823 6 15 9.281063081779141 6 16 8.378006249380494 6 17 9.74395025910868 6 18 10.399990554353355 6 19 8.878331715247274 6 20 8.168035790665858 6 21 7.993528970386571 6 22 8.616180227292485 6 23 7.653708658302417 6 24 8.59458687583836 6 25 7.9219007896142415 6 26 7.014686318773148 6 27 6.924600920610288 6 28 6.751861344419591 6 29 7.263786344575102 6 30 7.4742443467461825 6 31 6.102081239578471 6 32 6.024798601090268 6 33 6.230775362741099 6 34 5.699728634994031 6 35 6.088056591875802 6 36 7.670884510889735 6 37 7.656540494836765 6 38 7.94264110635681 6 39 8.596238246696323 6 40 6.617482045409716 6 41 12.964362794722824 6 42 12.112722666855074 6 43 11.807203788846063 6 44 12.499453166138291 6 45 11.801296202440529 6 46 13.863745705281033 6 47 14.300192924254315 6 48 13.383978340571268 6 49 13.888592606990183 6 50 13.541874553597108 6 51 13.057329637294174 6 52 13.222651743028596 6 53 13.059261984536501 6 54 13.167432436794694 6 55 13.196738404774571 6 56 12.443202406727412 7 1 3.7074326019640935 7 2 5.374817108303056 7 3 4.8952795654204735 7 4 6.322609275130716 7 5 6.08523064828524 7 6 7.596569697968633 7 7 5.790290153195016 7 8 6.065622352000069 7 9 6.070815268841422 7 10 6.322131194352416 7 11 6.975157912197552 7 12 7.585735566941484 7 13 8.319941433502358 7 14 7.60539126632386 7 15 8.715444633141825 7 16 8.426573684740386 7 17 9.201182963511194 7 18 10.122073014171658 7 19 8.707542057665162 7 20 7.881872268653279 7 21 7.882068453265681 7 22 8.773762337704785 7 23 7.719396988141693 7 24 8.149862906586094 7 25 7.521132096392719 7 26 6.709654872232267 7 27 6.173308118374088 7 28 6.222301595991919 7 29 6.44757390572115 7 30 6.555096897410053 7 31 4.9706346986979675 7 32 5.374623898085375 7 33 5.369000380211396 7 34 5.317757985098851 7 35 5.570726053790548 7 36 6.98954279181217 7 37 6.833060410979318 7 38 7.687714481618862 7 39 8.175972698128888 7 40 8.373876175388752 7 41 11.803845673828349 7 42 10.897278921222654 7 43 11.106085689614755 7 44 11.616294535809853 7 45 10.95056244981998 7 46 12.308190149725476 7 47 13.006272588824547 7 48 12.255816216182723 7 49 12.680626149967122 7 50 12.14383122725189 7 51 11.639999099208922 7 52 11.711959919129225 7 53 12.084209573635032 7 54 11.704050186619991 7 55 11.71525692329309 7 56 11.026500799459768 8 1 3.626854584593623 8 2 5.399824595574692 8 3 4.904683578598068 8 4 6.336880014873159 8 5 5.92714439491597 8 6 7.471083025312154 8 7 6.071212585060849 8 8 5.8842656583792206 8 9 6.11207013556799 8 10 6.335159047002911 8 11 6.59970118862762 8 12 7.829925444150231 8 13 8.420615287949794 8 14 7.700584437932125 8 15 8.562809113525173 8 16 8.441233762608011 8 17 9.184426449584869 8 18 9.799461453824254 8 19 8.253199440115 8 20 7.798988171618237 8 21 7.979507806365412 8 22 8.401629962742224 8 23 7.66429066888443 8 24 8.179242960494449 8 25 7.473752641466099 8 26 6.259924414011248 8 27 6.053436899446268 8 28 6.0097978523560975 8 29 6.74447417526293 8 30 6.927154399656239 8 31 5.342557430054665 8 32 5.3396134159050845 8 33 5.500881182846918 8 34 5.726712761978156 8 35 5.492289396108607 8 36 6.682789272794501 8 37 6.160508748805018 8 38 6.917244280959984 8 39 7.239359939818016 8 40 2.525418553346224 8 41 10.954309884669918 8 42 10.136796740929153 8 43 10.005351936994206 8 44 10.491781208466332 8 45 9.655264456408784 8 46 12.16533300686833 8 47 12.22082784488924 8 48 12.017047123640047 8 49 12.38621165460923 8 50 12.038170849893401 8 51 11.716324346288886 8 52 11.774239044615893 8 53 11.422754048028562 8 54 11.50288217224443 8 55 11.29462200265817 8 56 11.137911401436408 9 1 3.789929432451075 9 2 5.337304989576829 9 3 4.878082160692301 9 4 6.420558413768944 9 5 6.095552074193215 9 6 7.758177573276264 9 7 6.074725979878586 9 8 6.0730949450345975 9 9 6.185365197993484 9 10 6.545115756959625 9 11 7.066559209833471 9 12 7.960752346716266 9 13 8.757289483319646 9 14 7.720582555756334 9 15 8.730777618450201 9 16 8.26401122668982 9 17 9.677415344747672 9 18 10.177841527856497 9 19 8.564966175794776 9 20 7.666718267609202 9 21 7.93334744498766 9 22 8.582114936705455 9 23 7.749302275635163 9 24 8.344403886524937 9 25 7.611653388062493 9 26 6.69993006053863 9 27 6.402407976678212 9 28 6.28809322847756 9 29 6.721464576166376 9 30 6.730561137845584 9 31 5.558866483320241 9 32 5.940184342562965 9 33 5.544375128514773 9 34 5.492789680228988 9 35 6.074164043200643 9 36 7.426264396704404 9 37 7.346044227818755 9 38 7.395254193752507 9 39 8.29718979112613 9 40 5.021873788931895 9 41 11.307343324659875 9 42 11.63558901146055 9 43 11.322984787235757 9 44 12.063382312676136 9 45 10.740777976704909 9 46 13.632300821662234 9 47 14.089828409541976 9 48 13.32755907545635 9 49 13.623067793639283 9 50 12.978882312343995 9 51 13.009049736840367 9 52 13.055326314833604 9 53 12.793166244527715 9 54 12.827086952970948 9 55 12.889169966771348 9 56 12.605067433809833 10 1 3.7229653663890474 10 2 5.44543481753346 10 3 4.83427588578374 10 4 6.262701657499775 10 5 6.243830820523556 10 6 7.886086647784651 10 7 6.345993590132309 10 8 6.060564808744765 10 9 6.083106821334965 10 10 6.586986749038083 10 11 7.05088013876923 10 12 7.854442996676081 10 13 8.752713687475584 10 14 7.627328164468384 10 15 8.897015133415838 10 16 8.462853337132149 10 17 9.599413606273266 10 18 10.205968731700825 10 19 8.63225825589581 10 20 8.098187934510943 10 21 8.101766253312654 10 22 8.59544870739084 10 23 8.101687757733796 10 24 8.44213566944525 10 25 7.827371820811415 10 26 6.689236802867203 10 27 6.538140186016776 10 28 6.538859961293728 10 29 6.993585022921501 10 30 7.07287658894689 10 31 5.184930478859247 10 32 5.562066159104717 10 33 5.658376407769531 10 34 5.764090091886608 10 35 5.864251458942039 10 36 6.823489397934459 10 37 6.876918549239715 10 38 7.833005329501113 10 39 8.513465847741351 10 40 10.410750832869375 10 41 11.40869966271115 10 42 11.547008820228367 10 43 11.77725142984806 10 44 12.422213270641134 10 45 11.315081356889582 10 46 13.079974382090619 10 47 13.74187111696985 10 48 12.684454750591243 10 49 13.5348812192539 10 50 12.785058816698378 10 51 12.107798363281574 10 52 12.586413845379905 10 53 12.394301173102658 10 54 12.630426135887983 10 55 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5.379990976712165 115 8 5.271334850697031 115 9 5.03693639883204 115 10 5.526029870470424 115 11 3.212018122767759 115 12 0.09487764971017043 115 13 -0.1963933939760132 115 14 -2.5050851227098114 115 15 -4.366573505083963 115 16 3.2448161532069726 115 17 4.772495378814014 115 18 5.584868802767513 115 19 4.378802351629513 115 20 3.7269676507026865 115 21 6.129665659561937 115 22 7.390295226269362 115 23 6.3103487237822655 115 24 7.080979345656246 115 25 6.876453685051118 115 26 5.763673427428767 115 27 5.1994730302890275 115 28 5.551124696169129 115 29 5.6633770955470695 115 30 5.835468001340004 115 31 5.284217519504811 115 32 4.670798191841243 115 33 4.781092530737823 115 34 4.737543245184327 115 35 4.5960778728252585 115 36 6.505752528078197 115 37 6.426951790109936 115 38 6.577509123545269 115 39 7.571969779333934 115 40 9.714228515636847 115 41 10.691659704892665 115 42 10.08262240084321 115 43 8.25596581053626 115 44 1.4770263194020536 115 45 8.549853599616707 115 46 4.457361420443707 115 47 -5.988327640509343 115 48 3.443974248080984 115 49 -1.893605060290355 115 50 3.789552065363015 115 51 5.295185172387281 115 52 5.3202576362145955 115 53 5.407757994358477 115 54 5.548791143567769 115 55 5.422439243735081 115 56 8.33391245379105 116 1 3.496731391037592 116 2 4.846045870154178 116 3 4.696912908140831 116 4 6.0995547365031015 116 5 5.569113122705588 116 6 7.309983759735275 116 7 5.9700517227059535 116 8 5.533697072736011 116 9 5.546268192900371 116 10 5.7328136312246585 116 11 5.50085020544828 116 12 5.1729128878839425 116 13 5.57431109463068 116 14 6.2576206852668825 116 15 -2.846631777258999 116 16 4.797962026052691 116 17 6.23400001035246 116 18 5.947530631744176 116 19 5.801825358143905 116 20 5.329727332954416 116 21 6.276060755903437 116 22 7.462338325689391 116 23 6.505560510154272 116 24 7.437594062129129 116 25 6.352691281598766 116 26 5.586328109817924 116 27 5.308293568482043 116 28 5.134945921534015 116 29 5.709115255575654 116 30 5.601994484944079 116 31 4.8100414385834505 116 32 4.682067810598284 116 33 5.201366849750492 116 34 4.847655666503151 116 35 4.5125452596480535 116 36 6.428761791155075 116 37 6.404467526345886 116 38 6.7841004761743875 116 39 7.656381972511689 116 40 9.636055843087995 116 41 8.226994082727249 116 42 5.788952628159667 116 43 0.9734282222346735 116 44 1.6342237576252998 116 45 5.837448772921455 116 46 0.19873741340706985 116 47 -6.160835268117161 116 48 0.3477152117708222 116 49 -2.788687658349783 116 50 1.0779718449182774 116 51 4.586793429444537 116 52 4.575885573874366 116 53 4.898233792165023 116 54 3.9694777657056974 116 55 4.401824087472196 116 56 6.189084109325535 117 1 2.427474019359394 117 2 3.5945594000655987 117 3 3.5159882630734884 117 4 4.916051520873928 117 5 4.735506764253948 117 6 6.343808538281564 117 7 4.697906351998512 117 8 4.4812459138960605 117 9 4.253281904828533 117 10 4.454206666050531 117 11 1.0623415293167398 117 12 -6.089160889570252 117 13 -6.333740112747069 117 14 -1.209403947666015 117 15 -5.764329872150398 117 16 -8.04240928810821 117 17 0.4654949745558312 117 18 -3.628916401382874 117 19 0.08750064026985882 117 20 -0.9762731524235768 117 21 4.733863207062271 117 22 5.363108863245511 117 23 4.617349128040448 117 24 5.288127977916048 117 25 4.8138610425501085 117 26 4.794767619586157 117 27 4.330924317177583 117 28 4.229503620842355 117 29 4.7584114574928975 117 30 5.402473795707337 117 31 3.806552784293917 117 32 4.021862738398305 117 33 4.462166509985906 117 34 4.4405145428531085 117 35 4.304136086004075 117 36 5.2515078171228 117 37 5.485131280744621 117 38 6.000093680882557 117 39 6.156897686624056 117 40 8.494446815057415 117 41 6.63152629969121 117 42 4.709289965861402 117 43 -5.255488171406874 117 44 -1.5760662557225942 117 45 5.034087530353805 117 46 -2.716200094176962 117 47 -2.3427610052849808 117 48 -0.5209900424458986 117 49 -1.5855266342022247 117 50 0.44670743525925616 117 51 5.69657986312977 117 52 5.88931840969526 117 53 5.716308054997203 117 54 6.392545354590542 117 55 5.721354484268701 117 56 8.50521686020528 118 1 2.4712572579436625 118 2 3.8229657818737763 118 3 3.4151263521987403 118 4 5.078943506936646 118 5 4.594437627998673 118 6 6.399236742939557 118 7 4.629274406280558 118 8 4.650556385196268 118 9 4.753465290121035 118 10 4.75495854574978 118 11 1.3989910800227747 118 12 -5.281394522432894 118 13 -5.6714899752081 118 14 -8.495530850288425 118 15 -8.49755011788669 118 16 -8.802082862287563 118 17 -0.33750337497653504 118 18 -7.109477560377922 118 19 -1.3660570344046334 118 20 -1.176575738156199 118 21 3.7671017913278178 118 22 5.410193037094768 118 23 4.976738455016427 118 24 5.040145216133546 118 25 4.761137770956488 118 26 4.6866161433345574 118 27 4.8243224466476065 118 28 4.794467860710316 118 29 4.847857633909548 118 30 5.625149387124907 118 31 3.3995749739143157 118 32 4.246463875387121 118 33 3.4296614668903587 118 34 3.3637219516189205 118 35 4.420860824680037 118 36 5.2406422385422005 118 37 4.642338322739857 118 38 4.906383796415291 118 39 5.825199538815511 118 40 8.331600675317823 118 41 6.489815314912189 118 42 5.165827651539008 118 43 -1.099702365428513 118 44 -0.5247183738227221 118 45 5.049583587570016 118 46 -1.1279000758384328 118 47 -1.5084954633814398 118 48 -1.115158390144412 118 49 -1.5613656219154102 118 50 -0.40543817266298987 118 51 4.475096276113447 118 52 4.363002620221575 118 53 3.7986938976526226 118 54 4.586842071993804 118 55 5.076159178932187 118 56 6.5313951107095924 119 1 2.642366850105887 119 2 4.0672127629628605 119 3 3.7442218181363103 119 4 5.234787662780803 119 5 4.922166039937615 119 6 6.56054911477824 119 7 5.2207075345557925 119 8 4.997469121582682 119 9 5.061781535279387 119 10 5.266327878171744 119 11 1.8420532809797123 119 12 -5.992080327855543 119 13 -1.1341466168121102 119 14 -4.627770540422851 119 15 -5.638128837412776 119 16 -7.444552673178425 119 17 0.7954654106238301 119 18 -4.02189496753217 119 19 0.22715326406882497 119 20 -0.4531763305462668 119 21 5.258512135369742 119 22 5.979479890592149 119 23 4.9358635404573015 119 24 4.661926943178429 119 25 5.120217287930743 119 26 5.884383288470122 119 27 5.3673618633712294 119 28 5.248831041389284 119 29 5.844257725046482 119 30 5.673907646409482 119 31 4.474808512305746 119 32 4.956785133076799 119 33 4.420000951966685 119 34 4.780536715802107 119 35 5.009605413424627 119 36 5.878965318970543 119 37 6.100984939281208 119 38 6.629509793225495 119 39 7.567282017740091 119 40 8.928593156520826 119 41 6.780861111221142 119 42 5.737301791434202 119 43 -2.7305066488643774 119 44 0.07049693718206385 119 45 5.799276000420324 119 46 -0.303931062395737 119 47 -1.406877782816391 119 48 -4.0537320971391715 119 49 -1.2891851707875936 119 50 1.1957466074691574 119 51 7.242514822479361 119 52 7.4165911369679876 119 53 7.512797338071852 119 54 7.084267453629714 119 55 7.6182872263233925 119 56 10.2314178949429 120 1 2.4962560606829953 120 2 4.149243259006099 120 3 3.652231854808768 120 4 5.1635658909220865 120 5 4.897344576899592 120 6 6.641035507366546 120 7 5.21487854401343 120 8 4.830049704481738 120 9 4.999669973804771 120 10 4.830002844394478 120 11 1.2880289613502995 120 12 -5.4912503722485155 120 13 -3.190622200676227 120 14 -4.602949077384828 120 15 0.8359109232511877 120 16 -8.192246168642622 120 17 0.54755960093458 120 18 -5.667832045316381 120 19 0.10470586009275706 120 20 0.2136833490522676 120 21 3.9888344669659546 120 22 6.057958948689045 120 23 4.6180870494324004 120 24 5.003173806718287 120 25 4.305316689590654 120 26 5.6004612656945945 120 27 5.120189502823074 120 28 5.0682095397486755 120 29 5.85935133631844 120 30 5.673135594682016 120 31 4.622888033633675 120 32 4.806350853980099 120 33 4.250072366751475 120 34 4.563281359693249 120 35 4.12695727599942 120 36 5.743779061491296 120 37 5.7697747481984045 120 38 5.611714000907412 120 39 6.717446078732181 120 40 7.7782746851832485 120 41 -4.645427250099065 120 42 -2.1146186872378685 120 43 -5.805511474187675 120 44 -13.523712674861944 120 45 -2.709814908670584 120 46 -7.36059965919172 120 47 -5.386997450834148 120 48 -3.8883972197023837 120 49 -13.472624661233452 120 50 -2.6688355195333493 120 51 2.5083322322458166 120 52 1.8702101294404834 120 53 2.1625174693206475 120 54 2.1190483839520446 120 55 2.543282401000095 120 56 5.997177400829795 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 7221.0 5 2164.0 6 617.0 7 394.0 8 563.0 9 455.0 10 367.0 11 376.0 12 343.0 13 392.0 14 509.0 15 698.0 16 890.0 17 1167.0 18 1427.0 19 1753.0 20 2133.0 21 2734.0 22 3212.0 23 3687.0 24 4291.0 25 4706.0 26 5112.0 27 5248.0 28 5467.0 29 5974.0 30 6726.0 31 7576.0 32 8609.0 33 9679.0 34 10548.0 35 11817.0 36 12916.0 37 14458.0 38 14608.0 39 13514.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 100.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 100.0 0.0 0.0 7 99.73600538432974 0.19146862235425177 0.048737467508355 0.023788525807649463 8 99.65941793538803 0.2564519123653918 0.0342322688451541 0.04989788340141107 9 98.89990658489461 0.3765615118161405 0.4194976472158237 0.30403425607343243 10 47.05834571110286 28.158652060898625 16.645585777942813 8.1374164500557 11 32.06867341255106 28.25206554028964 19.785090976606014 19.894170070553287 12 27.700867991088007 20.133795952469367 20.539361307092463 31.625974749350167 13 23.140433531377646 14.607315261789825 23.55296138135908 38.69928982547345 14 21.768241737838842 25.43399554400297 29.65790939472707 23.13985332343112 15 33.83776533706555 18.216701656169814 23.46920923722883 24.476323769535806 16 33.508868696838626 24.560048770575087 21.378349348274163 20.55273318431213 17 26.208329464202805 18.998398179960997 34.183768223604794 20.609504132231404 18 23.085313776457483 19.383006869662086 29.946272744151504 27.585406609728924 19 29.52031019631061 21.88439615040806 21.097876687679214 27.497416965602106 20 23.83978622672747 28.060646547968283 27.016759127479737 21.08280809782451 21 22.882953971836542 20.598662032712316 29.390023846684965 27.128360148766177 22 21.029637021908652 19.563451541032308 29.25408466394356 30.152826773115482 23 20.186014667656888 21.74735425176383 27.882473078351282 30.184158002228003 24 20.374378405352243 21.49542471524147 29.76227087310475 28.367926006301538 25 29.182696492296976 22.976760379493427 26.094525198015496 21.746017930194096 26 29.04501198887618 22.40787724319711 20.79445899107656 27.752651776850147 27 20.693859776455216 25.865002177384234 21.15256205545072 32.28857599070983 28 23.797035016971773 30.52946125504076 21.968145289970696 23.70535843801677 29 33.92088149659152 24.52940937171284 19.84471526288567 21.704993868809968 30 27.602022747197243 20.81269848642309 31.072160519272412 20.51311824710725 31 27.59142979839599 29.977135296370665 22.30701377685442 20.124421128378927 32 34.52260052576907 23.80642877454024 21.208340345521968 20.462630354168724 33 28.47124580220087 23.629687300277723 21.830298523071917 26.06876837444949 34 23.144180963066745 28.018231180201496 28.815263962607478 20.02232389412428 35 22.46748265160233 24.279620136927388 34.91648359312341 18.336413618346874 36 26.161919910831426 29.184130780573327 24.651975525084467 20.001973783510778 37 26.535213886806485 22.777012616444964 30.67987005817163 20.007903438576914 38 32.511558928727204 23.974526738201167 23.34234784378726 20.171566489284366 39 25.92629181097604 23.511192208456336 21.17396155365984 29.38855442690779 40 21.53117046485095 18.54840125208437 28.089986250475384 31.8304420325893 41 18.601104946453496 16.346052338334136 24.95630652293761 40.096536192274755 42 26.245489465719942 25.82120824118263 23.00605284600163 24.927249447095797 43 18.720071682502372 21.174266447119606 31.78816424136988 28.31749762900814 44 20.776587003269288 22.95086743940745 24.86290552865143 31.40964002867183 45 23.92711538126007 22.111978969774867 31.23186172161705 22.729043927348016 46 20.039579756118854 26.368848403713514 24.096504758301563 29.49506708186607 47 17.639715319099288 26.79850659199627 24.109205460273014 31.45257262863143 48 23.123951299662675 27.469126842664473 25.090427724588793 24.316494133084067 49 20.15348274337313 27.859037911052155 23.099211059135655 28.88826828643906 50 26.486458200086055 24.913655762678356 24.59909527519682 24.000790762038772 51 20.560687820524016 29.62587825763 26.98135503437356 22.832078887472424 52 21.189081101190478 26.91650390625 30.372837611607146 21.52157738095238 53 21.260969761865965 25.078563281268185 30.732209781419495 22.928257175446358 54 21.412869006909066 27.82701747039803 27.26114871938028 23.49896480331263 55 20.269106315617943 28.09728332984147 27.071257303815443 24.562353050725143 56 25.264235563751008 21.656554845239686 28.957425644664976 24.12178394634433 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 1.0 3 1.0 4 3.5 5 6.0 6 50.0 7 94.0 8 134.5 9 175.0 10 215.0 11 255.0 12 264.5 13 283.0 14 292.0 15 311.0 16 330.0 17 337.0 18 344.0 19 360.5 20 377.0 21 432.0 22 569.5 23 652.0 24 754.0 25 856.0 26 965.5 27 1075.0 28 1228.5 29 1596.0 30 1810.0 31 2220.5 32 2631.0 33 2848.5 34 3066.0 35 3203.5 36 3341.0 37 3667.5 38 4591.5 39 5189.0 40 6527.0 41 7865.0 42 11954.5 43 16044.0 44 15348.5 45 14653.0 46 12585.5 47 10237.0 48 9956.0 49 10085.0 50 10214.0 51 10243.0 52 10272.0 53 9922.0 54 9814.0 55 10056.0 56 10480.5 57 10905.0 58 10010.0 59 9115.0 60 7986.5 61 6858.0 62 6463.0 63 5008.5 64 3949.0 65 3572.5 66 3196.0 67 2731.5 68 2267.0 69 2044.0 70 1821.0 71 1319.5 72 624.0 73 430.0 74 408.0 75 386.0 76 479.0 77 572.0 78 360.0 79 89.5 80 31.0 81 17.0 82 3.0 83 2.0 84 1.0 85 0.5 86 0.0 87 0.5 88 0.5 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0017406238395841068 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5059413293724471 16 0.06788432974378017 17 0.02784998143334571 18 0.0 19 0.04293538804307464 20 0.12010304493130339 21 5.802079465280356E-4 22 0.0 23 0.0 24 0.007542703304864464 25 0.009863535090976606 26 0.06324266617155588 27 0.07368640920906053 28 0.004061455625696249 29 0.163038432974378 30 0.06440308206461196 31 0.019727070181953212 32 0.019146862235425176 33 0.13808949127367248 34 0.1966904938730041 35 0.16826030449313034 36 0.05453954697363535 37 0.15955718529520982 38 0.2361446342369105 39 0.15549572966951355 40 0.8337588191607872 41 1.071063869290754 42 0.30867062755291497 43 0.28024043817304123 44 0.43805699962866695 45 0.23730505012996655 46 0.3173737467508355 47 0.5407538061641292 48 0.0667239138507241 49 0.27617898254734496 50 0.21235610842926106 51 0.3266570738952841 52 0.18566654288897141 53 0.2993873004084664 54 0.2344040103973264 55 0.3040289639806907 56 0.25587170441886375 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 172352.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.69624736474346 #Duplication Level Percentage of deduplicated Percentage of total 1 87.31469979030626 56.48933416211966 2 7.626120935626515 9.867628129694834 3 2.1545223569464182 4.1816853407362675 4 0.8731375102636727 2.259548813898191 5 0.49141086624067765 1.5896219480014868 6 0.2806561539863239 1.0894439977642043 7 0.22871903587822628 1.035808432554235 8 0.14182571994035034 0.7340473487954987 9 0.12784289599649157 0.744386006089277 >10 0.6521985194786063 7.815406839644003 >50 0.06392144753699985 3.0408685064049683 >100 0.03895213209285928 5.106668892077872 >500 0.0019975452355312454 0.7023850544968552 >1k 0.003995090471062491 5.3431665277226275 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 4643 2.6939054957296693 No Hit ACAATAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT 1317 0.7641338655774229 No Hit ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 1223 0.7095943186037875 No Hit ACAATAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC 1086 0.6301058299294466 No Hit ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 549 0.31853416264389156 No Hit ACAATAGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT 538 0.31215187523208315 No Hit ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG 497 0.28836334942443376 No Hit ACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 389 0.2257008911994059 No Hit ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG 385 0.2233800594132937 No Hit ACAATAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC 371 0.21525714816190122 No Hit ACAATAGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC 319 0.18508633494244336 No Hit ACAATAGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT 302 0.17522279985146677 No Hit ACAATAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT 301 0.17464259190493875 No Hit ACAATAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 299 0.17348217601188265 No Hit ACAATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 297 0.17232176011882658 No Hit ACAATAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT 271 0.15723635350909765 No Hit ACAATAGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT 262 0.15201448199034534 No Hit ACAATAGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC 255 0.14795302636464908 No Hit ACAATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 235 0.1363488674340884 No Hit ACAATAGGGATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT 204 0.11836242109171927 No Hit ACAATAGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA 202 0.1172020051986632 No Hit ACAATAGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC 199 0.1154613813590791 No Hit ACAATAGGGACTAATCCTAGCCCTAGCCCTACACAAATATCATTATACTATTATAT 186 0.10791867805421464 No Hit ACAATAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA 183 0.10617805421463053 No Hit ACAATAGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG 183 0.10617805421463053 No Hit ACAATAGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGG 180 0.10443743037504642 No Hit ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC 177 0.1026968065354623 No Hit ACAATAGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC 174 0.10095618269587821 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.004641663572224285 0.0 0.0 0.0 0.0 7 0.004641663572224285 0.0 0.0 0.0 0.0 8 0.004641663572224285 0.0 0.0 0.0 0.0 9 0.005221871518752321 0.0 0.0 0.0 0.0 10 0.010443743037504642 0.0 0.0 0.0 0.0 11 0.012764574823616785 0.0 0.0 0.0 0.0 12 0.013924990716672856 0.0 0.0 0.0 0.0 13 0.016245822502784998 0.0 0.0 0.0 0.0 14 0.0243687337541775 0.0 0.0 0.0 0.0 15 0.0243687337541775 0.0 0.0 0.0 0.0 16 0.02552914964723357 0.0 0.0 0.0 0.0 17 0.028430189379873745 0.0 0.0 0.0 0.0 18 0.030170813219457852 0.0 0.0 0.0 0.0 19 0.03191143705904196 0.0 0.0 0.0 0.0 20 0.03307185295209803 0.0 0.0 0.0 0.0 21 0.03481247679168214 0.0 0.0 0.0 0.0 22 0.036553100631266246 0.0 0.0 0.0 0.0 23 0.03887393241737839 0.0 0.0 0.0 0.0 24 0.04003434831043446 0.0 0.0 0.0 0.0 25 0.04119476420349053 0.0 0.0 0.0 0.0 26 0.04409580393613071 0.0 0.0 0.0 0.0 27 0.04815725956182696 0.0 0.0 0.0 0.0 28 0.05337913108057928 0.0 0.0 0.0 0.0 29 0.056280170813219454 0.0 0.0 0.0 0.0 30 0.05686037875974749 0.0 0.0 0.0 0.0 31 0.06266245822502785 0.0 0.0 0.0 0.0 32 0.06440308206461196 0.0 0.0 0.0 0.0 33 0.06614370590419606 0.0 0.0 0.0 0.0 34 0.06788432974378017 0.0 0.0 0.0 0.0 35 0.07136557742294838 0.0 0.0 0.0 0.0 36 0.07252599331600446 0.0 0.0 0.0 0.0 37 0.07426661715558856 0.0 0.0 0.0 0.0 38 0.0765874489417007 0.0 0.0 0.0 0.0 39 0.07832807278128481 0.0 0.0 0.0 0.0 40 0.07948848867434088 0.0 0.0 0.0 0.0 41 0.08180932046045303 0.0 0.0 0.0 0.0 42 0.08703119197920535 0.0 0.0 0.0 0.0 43 0.09167285555142964 0.0 0.0 0.0 0.0 44 0.10037597474935017 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCGAC 15 0.007993065 50.660557 41 TAGACCT 20 3.9809992E-4 50.481438 37 GGCGCAT 70 0.0 50.481434 37 CGTATCT 15 0.0081531275 50.40718 36 GCGTATC 15 0.008191133 50.34793 35 TAAGAGA 15 0.008238825 50.274063 34 ACTCGAA 15 0.008248389 50.259315 46 TATACTA 90 0.0 50.20041 44 TAGTGGT 20 4.127879E-4 50.112316 15 AGATTAC 40 2.6138878E-9 50.09766 49 GATTACG 40 2.6411726E-9 50.03914 50 ATAATAG 15 0.008519527 49.849873 16 CGCTGAT 15 0.008519527 49.849873 28 TAGCTAT 15 0.008519527 49.849873 31 TAATAGC 15 0.008548966 49.806396 17 AGGGACC 300 0.0 49.705254 6 TAGGGTA 1255 0.0 49.309193 5 AGGGTAC 1115 0.0 49.259464 6 GGGTACC 1070 0.0 49.008446 7 TAGGGAC 955 0.0 48.924545 5 >>END_MODULE