FastQCFastQC Report
Fri 17 Jun 2016
SRR1525997_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525997_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5180
Sequences flagged as poor quality0
Sequence length40
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT420.8108108108108109No Hit
ACAATAGGGTACCTGGTTGATCCTGCCCGTAGCATATGCT210.40540540540540543No Hit
ACAATAGGGTACCTGGTTGATCCTGCCCGTAGCCTATGCT190.3667953667953668No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCCTATGCT170.3281853281853282No Hit
ACAATAGGGATGAGATGGACTTAAAGGAATTGACGGGTAC150.28957528957528955No Hit
ACAATAGGGATGAGATGGACTTAAATGAATTGACGGGTAC150.28957528957528955No Hit
ACAATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC140.2702702702702703No Hit
ACAATAGGGATGAGATGGACTCAAAGGAATTGACGGGTAC130.25096525096525096No Hit
ACAATAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT130.25096525096525096No Hit
ACAATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG100.19305019305019305No Hit
ACAATAGGGTACCTGGTTGATCCTGCCCGTAGCCTCTGCT90.17374517374517373No Hit
ACAATAGGGGTTGGGTGCGTTGATTTAGATACGTTTATGT90.17374517374517373No Hit
ACAATAGGGATGAGATGGCCTTAAATGAATTGACGGGTAC80.15444015444015444No Hit
ACAATAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT80.15444015444015444No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATCTGCT70.13513513513513514No Hit
ACAATAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT70.13513513513513514No Hit
ACAATAGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCG70.13513513513513514No Hit
ACAATAGGGTACCTGGTTCATCCTGCCCGTAGCCTATGCT70.13513513513513514No Hit
ACAATAGGGTACCTGGTTGATCCTGCCCGTCGCCTCTGCT60.11583011583011582No Hit
ACAATAGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCC60.11583011583011582No Hit
ACAATAGGGTACCTGGTTCATCCTGCCAGTAGCATATGCT60.11583011583011582No Hit
ACAATAGGGTACCTGGTTGATCCTGCCCGTAGCATCTGCT60.11583011583011582No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTGC251.6814559E-434.26470620
CCTGCCC251.6814559E-434.26470622
TGCCCGT251.6814559E-434.26470624
CCCGTAG251.6814559E-434.26470626
CTGCCCG251.6814559E-434.26470623
GCCCGTA251.6814559E-434.26470625
TCCTGCC251.6814559E-434.26470621
ATAGGGG1750.033.932044
TAGGGGA358.517836E-733.932045
TAGGGCA358.517836E-733.932045
GTTGATC200.002565307333.93203716
AGGGGGG502.6921043E-1033.9320376
AGGGGCT251.7832794E-433.9320376
TGGTTGA200.002565307333.93203714
AGGGGAC200.002565307333.9320376
GGATGAG301.2351124E-533.9320378
GGTTGAT200.002565307333.93203715
GTACCTG251.7832794E-433.9320379
GGGTACC251.7832794E-433.9320377
ATAGGGT750.033.9320374
Produced by FastQC (version 0.11.5)