##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525997_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5180
Sequences flagged as poor quality	0
Sequence length	40
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	21.65212355212355	24.0	17.0	30.0	4.0	32.0
2	11.473166023166023	11.0	10.0	12.0	4.0	17.0
3	18.22123552123552	20.0	10.0	25.0	4.0	30.0
4	19.36042471042471	22.0	10.0	27.0	4.0	31.0
5	19.671042471042472	22.0	13.0	27.0	4.0	31.0
6	19.274517374517373	20.0	10.0	28.0	4.0	32.0
7	18.475096525096525	20.0	9.0	27.0	4.0	31.0
8	20.515444015444015	24.0	11.0	29.0	4.0	32.0
9	19.13957528957529	22.0	9.0	27.0	4.0	31.0
10	17.123745173745174	18.0	7.0	25.0	4.0	30.0
11	22.585521235521234	26.0	10.0	34.0	4.0	37.0
12	21.64787644787645	24.0	10.0	33.0	4.0	36.0
13	22.592277992277992	27.0	9.0	33.0	4.0	36.0
14	22.826061776061778	27.0	4.0	34.0	4.0	37.0
15	21.431853281853282	25.0	4.0	33.0	4.0	36.0
16	19.398262548262547	21.0	4.0	32.0	4.0	35.0
17	19.758880308880308	22.0	4.0	32.0	4.0	36.0
18	19.66872586872587	23.0	4.0	32.0	4.0	36.0
19	17.357915057915058	17.0	4.0	29.0	4.0	34.0
20	18.43320463320463	19.0	4.0	31.0	4.0	35.0
21	16.57972972972973	14.0	4.0	30.0	4.0	34.0
22	15.82953667953668	12.0	4.0	28.0	4.0	34.0
23	16.094980694980695	12.0	4.0	29.0	4.0	34.0
24	15.60965250965251	10.0	4.0	29.0	4.0	34.0
25	14.997683397683398	4.0	4.0	28.0	4.0	33.0
26	13.102316602316602	4.0	4.0	24.0	4.0	33.0
27	13.156949806949807	4.0	4.0	25.0	4.0	33.0
28	12.82027027027027	4.0	4.0	24.0	4.0	33.0
29	12.633397683397684	4.0	4.0	25.0	4.0	33.0
30	12.104826254826255	4.0	4.0	23.0	4.0	33.0
31	11.642664092664093	4.0	4.0	22.0	4.0	32.0
32	11.155212355212354	4.0	4.0	19.0	4.0	32.0
33	10.37084942084942	4.0	4.0	15.0	4.0	31.0
34	9.812741312741313	4.0	4.0	13.0	4.0	30.0
35	9.463127413127413	4.0	4.0	9.0	4.0	30.0
36	9.202509652509653	4.0	4.0	4.0	4.0	31.0
37	9.142471042471042	4.0	4.0	4.0	4.0	31.0
38	8.865637065637065	4.0	4.0	4.0	4.0	30.0
39	8.505791505791505	4.0	4.0	4.0	4.0	28.0
40	8.484749034749035	4.0	4.0	4.0	4.0	28.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	4.376084647675555
1	2	14.734853940251666
1	3	-5.865665489813214
1	4	4.917800633709721
1	5	5.337191280941283
1	6	8.187791722166725
1	7	7.2491964790260255
1	8	3.549557481659754
1	9	1.5250539864176211
1	10	4.588883149678605
1	11	-4.116949401608494
1	12	0.9755779258620159
1	13	-7.458010865397229
1	14	-1.0556707497048379
1	15	-4.055201017416923
1	16	-6.76839038486766
1	17	-4.584757319700506
1	18	-7.483826747747193
1	19	-4.114506768484047
1	20	-3.1162292568542593
1	21	-1.1024344973208606
1	22	-1.1531674133946836
1	23	-2.3709692769351847
1	24	-0.6895625523466418
1	25	-2.5290962825053764
1	26	-2.0422722479540667
1	27	-0.1664304760895714
1	28	-0.4395173008809401
1	29	-0.31679056423374874
1	30	0.7348161308388566
1	31	-1.0842812490539782
1	32	0.10007486200667692
1	33	0.014246422769151224
1	34	1.1337755994006002
1	35	-2.351405349700805
1	36	-0.02455915296824429
1	37	-0.1233492832924652
1	38	-0.18695386053340624
1	39	3.2420200695768875
1	40	0.38141873908919344
6	1	4.501084647675555
6	2	3.984853940251666
6	3	5.509334510186786
6	4	0.29280063370972087
6	5	0.5871912809412834
6	6	6.562791722166725
6	7	5.457529812359358
6	8	-1.2837758516735782
6	9	5.650053986417621
6	10	7.2555498163452725
6	11	6.341383931724838
6	12	8.725577925862016
6	13	10.375322467936105
6	14	11.569329250295162
6	15	7.569798982583077
6	16	7.189942948465674
6	17	10.748576013632828
6	18	4.807839918919473
6	19	9.093826564849287
6	20	7.925437409812407
6	21	10.730898836012473
6	22	10.638499253271982
6	23	12.08736405639815
6	24	14.685437447653358
6	25	15.304237050827957
6	26	12.332727752045933
6	27	7.208569523910429
6	28	10.393816032452394
6	29	7.308209435766251
6	30	4.3181494641721905
6	31	4.832385417612688
6	32	6.308408195340011
6	33	7.305913089435817
6	34	9.5087755994006
6	35	6.773594650299195
6	36	6.392107513698422
6	37	8.293317383374202
6	38	7.604712806133261
6	39	4.3670200695768875
6	40	5.006418739089193
7	1	5.914546186137095
7	2	6.657930863328589
7	3	8.07664220249448
7	4	7.879339095248181
7	5	4.356422050172053
7	6	11.283945568320572
7	7	6.101760581590128
7	8	2.0816087637110385
7	9	3.851977063340698
7	10	10.755549816345273
7	11	5.3894608548017615
7	12	6.0332702335543225
7	13	9.721476314089951
7	14	9.694329250295162
7	15	12.733260521044617
7	16	6.007250640773368
7	17	12.152422167478981
7	18	5.836686072765627
7	19	9.276518872541594
7	20	12.300437409812407
7	21	14.634744989858627
7	22	9.3788838686566
7	23	5.866210210244303
7	24	7.637360524576435
7	25	7.131160127751034
7	26	1.7654200597382417
7	27	3.7951079854488903
7	28	3.8745852632216238
7	29	4.490901743458558
7	30	3.2989186949414204
7	31	-0.34069150546423543
7	32	2.5295620414938575
7	33	1.142451550974279
7	34	0.6722371378621386
7	35	-0.5629438112392666
7	36	-0.954046332455424
7	37	-0.8797595397027216
7	38	-0.32797950155904676
7	39	0.7227893003461174
7	40	-0.02242741475696075
9	1	7.518941790532697
9	2	3.163425368823095
9	3	5.741477367329644
9	4	10.346372062281148
9	5	7.301476995226999
9	6	7.72350600788101
9	7	8.046815526645073
9	8	5.109081291183564
9	9	6.846482557846194
9	10	10.255549816345273
9	11	5.323526788867696
9	12	10.368435068719158
9	13	6.661036753650389
9	14	6.622900678866589
9	15	4.480513268297361
9	16	5.386371519894247
9	17	11.070004585061401
9	18	10.325697061776616
9	19	8.897397993420714
9	20	8.943294552669549
9	21	16.052327407441048
9	22	12.477784967557698
9	23	14.855221199255292
9	24	14.989008876224785
9	25	14.054237050827957
9	26	2.886299180617362
9	27	3.6907123810532863
9	28	1.215244603880965
9	29	-0.7453619928051776
9	30	-1.2889933929706672
9	31	-0.06047172524445443
9	32	1.754836766768582
9	33	-0.7476583391356115
9	34	0.2766327422577426
9	35	0.39859465029919505
9	36	3.6063932279841353
9	37	4.543317383374202
9	38	1.1939985204189743
9	39	1.777734355291174
9	40	2.488561596232051
12	1	-1.1239153523244454
12	2	1.734853940251666
12	3	-0.1156654898132139
12	4	1.4178006337097209
12	5	-9.912808719058717
12	6	-0.5622082778332746
12	7	-8.667470187640642
12	8	1.9662241483264218
12	9	-8.224946013582379
12	10	-0.24445018365472748
12	11	12.466383931724838
12	12	4.725577925862016
12	13	12.875322467936105
12	14	11.694329250295162
12	15	2.1947989825830767
12	16	7.314942948465674
12	17	1.498576013632828
12	18	14.682839918919473
12	19	2.9688265648492873
12	20	2.3004374098124067
12	21	10.980898836012473
12	22	10.763499253271982
12	23	16.21236405639815
12	24	11.060437447653358
12	25	19.054237050827957
12	26	14.957727752045933
12	27	3.8335695239104286
12	28	6.143816032452394
12	29	10.183209435766251
12	30	9.56814946417219
12	31	13.582385417612688
12	32	1.1834081953400108
12	33	8.180913089435817
12	34	6.1337755994006
12	35	-1.601405349700805
12	36	5.892107513698422
12	37	11.043317383374202
12	38	-4.020287193866739
12	39	-3.5079799304231125
12	40	-3.8685812609108066
13	1	0.7649735365644439
13	2	-0.09847939308166787
13	3	-0.1156654898132139
13	4	-8.471088255179168
13	5	0.36496905871906193
13	6	1.4377917221667254
13	7	-0.05635907652953165
13	8	-0.25599807389579965
13	9	0.8306095419731783
13	10	1.9222164830119404
13	11	10.855272820613724
13	12	8.892244592528684
13	13	7.708655801269437
13	14	9.083218139184051
13	15	10.13924342702752
13	16	10.203831837354564
13	17	9.943020458077271
13	18	12.182839918919473
13	19	10.468826564849287
13	20	12.022659632034628
13	21	11.480898836012473
13	22	11.596832586605315
13	23	13.156808500842592
13	24	9.949326336542248
13	25	13.109792606383515
13	26	8.457727752045933
13	27	7.55579174613265
13	28	5.532704921341283
13	29	7.46098721354403
13	30	5.290371686394412
13	31	1.6379409731682433
13	32	1.5167415286733448
13	33	1.625357533880262
13	34	2.467108932733934
13	35	2.731927983632529
13	36	3.058774180365088
13	37	2.9877618278186464
13	38	0.6463794727999268
13	39	1.5475756251324428
13	40	2.2980854057558595
15	1	10.876084647675555
15	2	2.901520606918332
15	3	11.884334510186786
15	4	3.417800633709721
15	5	11.253857947607951
15	6	13.60445838883339
15	7	12.832529812359358
15	8	9.799557481659754
15	9	11.775053986417621
15	10	14.088883149678605
15	11	14.299717265058174
15	12	14.225577925862016
15	13	14.541989134602769
15	14	12.194329250295162
15	15	5.861465649249745
15	16	8.314942948465674
15	17	14.665242680299492
15	18	11.349506585586141
15	19	11.968826564849287
15	20	9.133770743145739
15	21	14.980898836012473
15	22	9.596832586605315
15	23	9.379030723064817
15	24	15.89377078098669
15	25	13.554237050827957
15	26	7.624394418712601
15	27	6.0002361905770965
15	28	9.143816032452394
15	29	3.1832094357662513
15	30	6.234816130838858
15	31	1.4157187509460218
15	32	3.5167415286733448
15	33	-0.8190869105641827
15	34	-3.8662244005993998
15	35	-2.268072016367472
15	36	-2.9412258196349113
15	37	0.8766507167075357
15	38	0.8130461394665947
15	39	1.3253534029102214
15	40	0.9647520724225274
16	1	7.709417981008887
16	2	4.234853940251666
16	3	5.801001176853454
16	4	6.751133967043053
16	5	7.837191280941283
16	6	7.104458388833393
16	7	2.0825298123593576
16	8	4.049557481659754
16	9	1.6083873197509533
16	10	12.255549816345273
16	11	8.383050598391506
16	12	6.892244592528684
16	13	5.5419891346027725
16	14	5.944329250295162
16	15	8.778132315916409
16	16	7.481609615132342
16	17	7.415242680299496
16	18	5.432839918919473
16	19	9.80215989818262
16	20	7.050437409812407
16	21	6.480898836012473
16	22	8.013499253271982
16	23	8.129030723064817
16	24	8.810437447653358
16	25	7.3042370508279575
16	26	2.7910610853792672
16	27	-0.6664304760895714
16	28	0.5604826991190599
16	29	2.1832094357662513
16	30	1.1514827975055244
16	31	2.415718750946022
16	32	2.0167415286733448
16	33	3.0975797561024834
16	34	1.8004422660672663
16	35	-1.101405349700805
16	36	-0.10789248630157822
16	37	-2.2900159499591313
16	38	0.8963794727999268
16	39	-0.9246465970897795
16	40	-2.9519145942441396
17	1	7.576084647675554
17	2	1.6681872735849996
17	3	5.11766784352012
17	4	10.151133967043055
17	5	3.9205246142746155
17	6	7.437791722166725
17	7	8.66586314569269
17	8	9.999557481659757
17	9	7.041720653084287
17	10	3.0222164830119382
17	11	7.866383931724837
17	12	9.792244592528682
17	13	8.275322467936103
17	14	7.227662583628497
17	15	8.794798982583078
17	16	9.514942948465674
17	17	14.53190934696616
17	18	13.949506585586139
17	19	17.302159898182623
17	20	12.43377074314574
17	21	5.54756550267914
17	22	10.963499253271982
17	23	11.179030723064814
17	24	6.2937707809866925
17	25	5.787570384161292
17	26	-0.6089389146207331
17	27	5.800236190577094
17	28	5.71048269911906
17	29	2.783209435766251
17	30	1.334816130838858
17	31	6.649052084279354
17	32	5.683408195340011
17	33	1.0142464227691512
17	34	3.067108932733934
17	35	0.9652613169658615
17	36	-0.10789248630157822
17	37	2.3099840500408693
17	38	3.513046139466594
17	39	2.292020069576888
17	40	1.9314187390891941
18	1	3.4475132191041276
18	2	1.5205682259659525
18	3	2.7414773673296438
18	4	6.132086347995436
18	5	5.444334138084141
18	6	6.22350600788101
18	7	8.832529812359358
18	8	10.609081291183564
18	9	5.9179111292747635
18	10	6.398406959202415
18	11	9.10924107458198
18	12	8.582720783004874
18	13	8.946751039364678
18	14	9.480043536009447
18	15	10.480513268297361
18	16	7.743514377037101
18	17	8.855718870775686
18	18	15.111411347490904
18	19	13.968826564849287
18	20	10.657580266955264
18	21	10.123755978869616
18	22	15.977784967557698
18	23	13.21236405639815
18	24	12.917580304796216
18	25	11.339951336542242
18	26	4.529156323474506
18	27	9.404998095339002
18	28	5.5009588895952515
18	29	7.468923721480536
18	30	9.639578035600763
18	31	3.582385417612688
18	32	6.326265338197153
18	33	8.180913089435817
18	34	3.7766327422577426
18	35	8.11288036458491
18	36	7.606393227984135
18	37	6.543317383374202
18	38	9.336855663276118
18	39	10.349162926719746
18	40	10.274275881946336
19	1	-0.40169313010222396
19	2	0.06818727358499999
19	3	3.1621122879645647
19	4	4.973356189265278
19	5	3.698302392052394
19	6	4.9933472777222825
19	7	0.7769742568038005
19	8	5.466224148326422
19	9	1.6639428753065104
19	10	4.644438705234162
19	11	3.133050598391506
19	12	2.1700223703064587
19	13	5.986433579047215
19	14	4.027662583628494
19	15	5.861465649249745
19	16	6.537165170687896
19	17	10.22079823585505
19	18	12.460617696697252
19	19	8.41327100929373
19	20	8.967104076479075
19	21	2.7031210582346947
19	22	3.874610364383093
19	23	6.379030723064817
19	24	1.1159930032089136
19	25	3.7209037174946253
19	26	-0.431161136842956
19	27	8.222458412799318
19	28	5.7549271435635045
19	29	0.9609872135440298
19	30	0.06814946417219048
19	31	1.6934965287237986
19	32	0.016741528673344774
19	33	-0.5413091327864041
19	34	2.244886710511711
19	35	2.6763724280769736
19	36	0.7809964025873111
19	37	-0.4011270610702429
19	38	-0.6869538605334062
19	39	0.9364645140213321
19	40	0.02030762797808272
25	1	3.648811920402828
25	2	0.7348539402516661
25	3	1.9297890556413329
25	4	3.326891542800631
25	5	2.31446400821401
25	6	3.165064449439452
25	7	6.968893448722994
25	8	0.4662241483264218
25	9	-3.134036922673287
25	10	2.391913452708909
25	11	0.8300202953612015
25	12	2.089214289498379
25	13	4.693504286117921
25	14	6.330692886658799
25	15	4.194798982583077
25	16	3.042215675738401
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106	31	-6.417614582387312
106	32	-5.316591804659989
106	33	-5.319086910564183
106	34	-4.8662244005994
106	35	-4.101405349700805
106	36	-4.107892486301578
106	37	-3.9566826166257982
106	38	-4.020287193866739
106	39	-3.5079799304231125
106	40	-3.8685812609108066
107	1	-2.3382010666101607
107	2	0.7348539402516661
107	3	0.3843345101867861
107	4	-5.867913652004566
107	5	0.015762709512710416
107	6	0.43779172216672535
107	7	-3.953184473354929
107	8	-5.390918708816436
107	9	-0.9392317278680942
107	10	-1.530164469369014
107	11	-3.3907589254180195
107	12	5.725577925862016
107	13	-0.6961061034924683
107	14	2.694329250295162
107	15	2.337656125440219
107	16	2.0292286627513896
107	17	3.998576013632828
107	18	1.968554204633758
107	19	-4.174030578007855
107	20	1.1575802669552644
107	21	-2.090529735416098
107	22	0.19207068184341125
107	23	-5.71620737217328
107	24	-5.296705409489499
107	25	-2.9457629491720425
107	26	-3.68512939081121
107	27	-3.0950019046610002
107	28	-2.6418982532618926
107	29	-4.531076278519463
107	30	-1.0747076786849519
107	31	0.7252425604698303
107	32	1.254836766768582
107	33	-0.6048011962784692
107	34	-2.1519386863136853
107	35	-1.8156910639865194
107	36	5.177821799412708
107	37	-0.24239690234008382
107	38	2.265427091847547
107	39	0.2063057838626019
107	40	-0.15429554662509215
108	1	-0.4239153523244461
108	2	-0.06514605974833465
108	3	-2.4156654898132146
108	4	1.6178006337097202
108	5	0.987191280941282
108	6	-0.7622082778332739
108	7	-6.467470187640643
108	8	-3.9337758516735786
108	9	-6.224946013582379
108	10	1.7555498163452725
108	11	0.6663839317248375
108	12	3.1255779258620144
108	13	0.8753224679361047
108	14	3.8943292502951614
108	15	-13.005201017416923
108	16	-5.085057051534326
108	17	-11.601423986367172
108	18	-8.717160081080527
108	19	-8.831173435150713
108	20	-3.8995625901875925
108	21	0.6808988360124726
108	22	0.7634992532719824
108	23	-4.287635943601851
108	24	0.7604374476533575
108	25	-0.7457629491720432
108	26	2.6577277520459326
108	27	1.533569523910428
108	28	2.843816032452393
108	29	-0.8167905642337487
108	30	-0.5318505358278092
108	31	4.382385417612689
108	32	5.483408195340012
108	33	1.0809130894358177
108	34	1.5337755994006006
108	35	-1.101405349700805
108	36	1.8921075136984218
108	37	2.6433173833742014
108	38	3.9797128061332607
108	39	7.4920200695768875
108	40	6.731418739089193
109	1	0.3760846476755546
109	2	-0.26514605974833394
109	3	-1.615665489813214
109	4	-8.58219936629028
109	5	0.5871912809412834
109	6	3.4377917221667254
109	7	9.332529812359358
109	8	9.466224148326422
109	9	8.775053986417621
109	10	10.755549816345273
109	11	3.466383931724838
109	12	-9.274422074137984
109	13	8.875322467936105
109	14	12.694329250295162
109	15	14.194798982583077
109	16	-13.685057051534326
109	17	-14.001423986367172
109	18	-14.317160081080527
109	19	-11.031173435150713
109	20	-12.699562590187593
109	21	-10.519101163987527
109	22	-10.236500746728018
109	23	-10.28763594360185
109	24	-10.439562552346642
109	25	-9.945762949172043
109	26	-7.542272247954067
109	27	-7.666430476089571
109	28	-7.356183967547606
109	29	-7.816790564233749
109	30	-6.9318505358278095
109	31	-6.417614582387312
109	32	-5.316591804659989
109	33	-5.319086910564183
109	34	-4.8662244005994
109	35	-4.101405349700805
109	36	-4.107892486301578
109	37	-3.9566826166257982
109	38	-4.020287193866739
109	39	-3.5079799304231125
109	40	-3.8685812609108066
111	1	-2.6239153523244454
111	2	5.734853940251666
111	3	14.384334510186786
111	4	11.41780063370972
111	5	7.587191280941283
111	6	-1.5622082778332746
111	7	14.332529812359358
111	8	10.466224148326422
111	9	13.775053986417621
111	10	2.7555498163452725
111	11	11.466383931724838
111	12	-0.27442207413798414
111	13	6.875322467936105
111	14	6.694329250295162
111	15	-1.8052010174169233
111	16	17.314942948465674
111	17	16.998576013632828
111	18	14.682839918919473
111	19	14.968826564849287
111	20	16.300437409812407
111	21	8.480898836012473
111	22	22.76349925327198
111	23	17.71236405639815
111	24	18.560437447653356
111	25	14.054237050827957
111	26	-2.5422722479540667
111	27	3.3335695239104286
111	28	-1.3561839675476062
111	29	19.18320943576625
111	30	13.06814946417219
111	31	4.582385417612688
111	32	1.6834081953400108
111	33	-1.3190869105641827
111	34	19.1337755994006
111	35	19.898594650299195
111	36	13.892107513698422
111	37	2.0433173833742018
111	38	21.97971280613326
111	39	2.4920200695768875
111	40	25.13141873908919
112	1	0.7094179810088868
112	2	6.401520606918334
112	3	-1.2823321564798817
112	4	-2.582199366290279
112	5	-1.4128087190587166
112	6	-5.228874944499941
112	7	2.9991964790260255
112	8	-0.20044251834024607
112	9	1.7750539864176211
112	10	-2.9111168503213936
112	11	-5.866949401608496
112	12	2.3922445925286837
112	13	-1.1246775320638953
112	14	1.36099591696183
112	15	-11.138534350750257
112	16	-6.685057051534326
112	17	-13.334757319700504
112	18	-9.317160081080527
112	19	-8.364506768484045
112	20	-6.699562590187593
112	21	-4.185767830654193
112	22	-6.236500746728018
112	23	-9.28763594360185
112	24	-4.439562552346642
112	25	-2.2790962825053764
112	26	0.7910610853792672
112	27	0.6669028572437625
112	28	1.6438160324523938
112	29	1.1832094357662513
112	30	-1.9318505358278095
112	31	4.915718750946022
112	32	5.016741528673345
112	33	5.014246422769151
112	34	5.467108932733934
112	35	6.231927983632529
112	36	6.892107513698422
112	37	7.043317383374202
112	38	6.979712806133261
112	39	7.4920200695768875
112	40	7.131418739089193
113	1	13.376084647675555
113	2	3.734853940251666
113	3	14.384334510186786
113	4	16.41780063370972
113	5	13.587191280941283
113	6	9.437791722166725
113	7	-1.6674701876406424
113	8	13.466224148326422
113	9	-2.224946013582379
113	10	16.755549816345273
113	11	11.466383931724838
113	12	9.725577925862016
113	13	-10.124677532063895
113	14	11.694329250295162
113	15	15.194798982583077
113	16	0.3149429484656743
113	17	11.998576013632828
113	18	0.6828399189194734
113	19	-4.031173435150713
113	20	13.300437409812407
113	21	-10.519101163987527
113	22	-10.236500746728018
113	23	-10.28763594360185
113	24	-10.439562552346642
113	25	-9.945762949172043
113	26	-7.542272247954067
113	27	-7.666430476089571
113	28	-7.356183967547606
113	29	-7.816790564233749
113	30	-6.9318505358278095
113	31	-6.417614582387312
113	32	-5.316591804659989
113	33	-5.319086910564183
113	34	-4.8662244005994
113	35	-4.101405349700805
113	36	-4.107892486301578
113	37	-3.9566826166257982
113	38	-4.020287193866739
113	39	-3.5079799304231125
113	40	-3.8685812609108066
117	1	12.376084647675555
117	2	3.734853940251666
117	3	-7.615665489813214
117	4	15.41780063370972
117	5	10.587191280941283
117	6	11.437791722166725
117	7	1.3325298123593576
117	8	5.466224148326422
117	9	-9.224946013582379
117	10	16.755549816345273
117	11	3.466383931724838
117	12	4.725577925862016
117	13	8.875322467936105
117	14	2.694329250295162
117	15	13.194798982583077
117	16	-13.685057051534326
117	17	-14.001423986367172
117	18	-14.317160081080527
117	19	-11.031173435150713
117	20	-12.699562590187593
117	21	-10.519101163987527
117	22	-10.236500746728018
117	23	-10.28763594360185
117	24	-10.439562552346642
117	25	-9.945762949172043
117	26	-7.542272247954067
117	27	-7.666430476089571
117	28	-7.356183967547606
117	29	-7.816790564233749
117	30	-6.9318505358278095
117	31	-6.417614582387312
117	32	-5.316591804659989
117	33	-5.319086910564183
117	34	-4.8662244005994
117	35	-4.101405349700805
117	36	-4.107892486301578
117	37	-3.9566826166257982
117	38	-4.020287193866739
117	39	-3.5079799304231125
117	40	-3.8685812609108066
119	1	13.376084647675555
119	2	11.734853940251666
119	3	17.384334510186786
119	4	16.41780063370972
119	5	13.587191280941283
119	6	14.437791722166725
119	7	9.332529812359358
119	8	8.466224148326422
119	9	17.77505398641762
119	10	18.755549816345273
119	11	14.466383931724838
119	12	-2.274422074137984
119	13	14.875322467936105
119	14	-17.305670749704838
119	15	-15.805201017416923
119	16	-13.685057051534326
119	17	-14.001423986367172
119	18	-14.317160081080527
119	19	-11.031173435150713
119	20	-12.699562590187593
119	21	-10.519101163987527
119	22	-10.236500746728018
119	23	-10.28763594360185
119	24	-10.439562552346642
119	25	-9.945762949172043
119	26	-7.542272247954067
119	27	-7.666430476089571
119	28	-7.356183967547606
119	29	-7.816790564233749
119	30	-6.9318505358278095
119	31	-6.417614582387312
119	32	-5.316591804659989
119	33	-5.319086910564183
119	34	-4.8662244005994
119	35	-4.101405349700805
119	36	-4.107892486301578
119	37	-3.9566826166257982
119	38	-4.020287193866739
119	39	-3.5079799304231125
119	40	-3.8685812609108066
120	1	13.376084647675555
120	2	15.734853940251666
120	3	16.384334510186786
120	4	16.41780063370972
120	5	15.587191280941283
120	6	7.437791722166725
120	7	1.3325298123593576
120	8	-7.533775851673578
120	9	11.775053986417621
120	10	2.7555498163452725
120	11	6.466383931724838
120	12	2.725577925862016
120	13	-5.124677532063895
120	14	5.694329250295162
120	15	13.194798982583077
120	16	14.314942948465674
120	17	-14.001423986367172
120	18	-14.317160081080527
120	19	-11.031173435150713
120	20	-12.699562590187593
120	21	-10.519101163987527
120	22	-10.236500746728018
120	23	-10.28763594360185
120	24	-10.439562552346642
120	25	-9.945762949172043
120	26	-7.542272247954067
120	27	-7.666430476089571
120	28	-7.356183967547606
120	29	-7.816790564233749
120	30	-6.9318505358278095
120	31	-6.417614582387312
120	32	-5.316591804659989
120	33	-5.319086910564183
120	34	-4.8662244005994
120	35	-4.101405349700805
120	36	-4.107892486301578
120	37	-3.9566826166257982
120	38	-4.020287193866739
120	39	-3.5079799304231125
120	40	-3.8685812609108066
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	1039.0
5	19.0
6	28.0
7	35.0
8	42.0
9	88.0
10	157.0
11	183.0
12	189.0
13	231.0
14	230.0
15	223.0
16	229.0
17	224.0
18	232.0
19	255.0
20	256.0
21	258.0
22	241.0
23	236.0
24	173.0
25	182.0
26	147.0
27	99.0
28	75.0
29	50.0
30	32.0
31	20.0
32	5.0
33	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.43907156673114	0.2901353965183753	0.09671179883945842	0.17408123791102514
8	97.95248213250918	0.5794861889124976	0.5022213637241646	0.9658103148541627
9	97.93196752995748	1.3142636258214146	0.28991109393119446	0.4638577502899111
10	33.51998455300251	36.590075304112766	15.775246186522496	14.114693956362231
11	26.891891891891888	36.62162162162162	20.463320463320464	16.023166023166024
12	36.42857142857142	16.91119691119691	19.15057915057915	27.50965250965251
13	27.16216216216216	21.003861003861005	22.83783783783784	28.996138996139
14	23.64864864864865	23.494208494208493	31.40926640926641	21.44787644787645
15	29.150579150579148	26.061776061776058	24.884169884169886	19.903474903474905
16	31.756756756756754	19.42084942084942	25.617760617760617	23.204633204633204
17	30.173745173745175	21.94980694980695	26.62162162162162	21.254826254826256
18	29.575289575289577	22.664092664092664	26.158301158301157	21.602316602316602
19	24.467673248161052	18.21525358110724	15.6794425087108	41.63763066202091
20	20.328185328185327	30.55984555984556	22.00772200772201	27.104247104247104
21	18.243243243243242	21.37065637065637	34.78764478764479	25.5984555984556
22	19.633204633204635	18.822393822393824	25.83011583011583	35.714285714285715
23	23.223938223938223	23.687258687258687	25.675675675675674	27.413127413127413
24	17.799227799227797	24.536679536679536	29.53667953667954	28.127413127413124
25	26.777434312210204	26.062596599690877	23.628284389489952	23.531684698608966
26	18.225931677018632	20.690993788819874	29.503105590062113	31.579968944099377
27	18.146718146718147	19.671814671814673	30.07722007722008	32.1042471042471
28	15.784389489953632	23.802163833075735	30.29366306027821	30.119783616692423
29	20.166279969064192	21.616395978344936	33.62335653518948	24.593967517401392
30	15.647969052224372	17.11798839458414	43.63636363636363	23.597678916827853
31	13.537033455811255	23.18700444788242	41.636047186230904	21.63991491007542
32	25.05791505791506	14.382239382239382	39.227799227799224	21.332046332046332
33	16.39312608611701	17.358563429233442	40.02703224560726	26.221278239042284
34	11.718146718146718	20.90733590733591	44.42084942084942	22.953667953667953
35	15.84942084942085	18.61003861003861	47.084942084942085	18.455598455598455
36	14.247104247104247	20.231660231660232	41.486486486486484	24.034749034749034
37	12.65211512458953	18.15723391925826	47.07359474599188	22.117056210160325
38	17.741312741312743	17.548262548262546	43.108108108108105	21.602316602316602
39	13.513513513513514	19.74903474903475	39.478764478764475	27.25868725868726
40	10.096525096525097	21.37065637065637	46.64092664092664	21.891891891891895
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	3.5
10	7.0
11	8.5
12	10.0
13	10.0
14	20.5
15	31.0
16	35.5
17	40.0
18	40.0
19	55.0
20	70.0
21	83.0
22	96.0
23	96.0
24	104.0
25	112.0
26	133.0
27	154.0
28	154.0
29	178.5
30	203.0
31	225.5
32	248.0
33	248.0
34	265.5
35	283.0
36	309.0
37	335.0
38	335.0
39	342.0
40	349.0
41	347.0
42	345.0
43	345.0
44	326.5
45	308.0
46	327.5
47	347.0
48	347.0
49	366.5
50	386.0
51	380.5
52	375.0
53	375.0
54	369.0
55	363.0
56	330.0
57	297.0
58	297.0
59	267.5
60	238.0
61	223.5
62	209.0
63	209.0
64	176.5
65	144.0
66	121.0
67	98.0
68	98.0
69	79.5
70	61.0
71	49.0
72	37.0
73	37.0
74	26.0
75	15.0
76	11.0
77	7.0
78	7.0
79	8.5
80	10.0
81	5.5
82	1.0
83	1.0
84	1.0
85	1.0
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.19305019305019305
8	0.05791505791505791
9	0.11583011583011582
10	0.019305019305019305
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.2702702702702703
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.07722007722007722
26	0.5405405405405406
27	0.0
28	0.07722007722007722
29	0.15444015444015444
30	0.19305019305019305
31	0.17374517374517373
32	0.0
33	0.019305019305019305
34	0.0
35	0.0
36	0.0
37	0.05791505791505791
38	0.0
39	0.0
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	5180.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.42471042471043
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.73014517506405	86.56370656370656
2	2.66865926558497	4.826254826254826
3	0.7472245943637916	2.027027027027027
4	0.29888983774551664	1.0810810810810811
5	0.08539709649871904	0.3861003861003861
6	0.08539709649871904	0.4633204633204633
7	0.08539709649871904	0.5405405405405406
8	0.04269854824935952	0.3088803088803089
9	0.04269854824935952	0.34749034749034746
>10	0.2134927412467976	3.4555984555984556
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT	42	0.8108108108108109	No Hit
ACAATAGGGTACCTGGTTGATCCTGCCCGTAGCATATGCT	21	0.40540540540540543	No Hit
ACAATAGGGTACCTGGTTGATCCTGCCCGTAGCCTATGCT	19	0.3667953667953668	No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCCTATGCT	17	0.3281853281853282	No Hit
ACAATAGGGATGAGATGGACTTAAAGGAATTGACGGGTAC	15	0.28957528957528955	No Hit
ACAATAGGGATGAGATGGACTTAAATGAATTGACGGGTAC	15	0.28957528957528955	No Hit
ACAATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC	14	0.2702702702702703	No Hit
ACAATAGGGATGAGATGGACTCAAAGGAATTGACGGGTAC	13	0.25096525096525096	No Hit
ACAATAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT	13	0.25096525096525096	No Hit
ACAATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG	10	0.19305019305019305	No Hit
ACAATAGGGTACCTGGTTGATCCTGCCCGTAGCCTCTGCT	9	0.17374517374517373	No Hit
ACAATAGGGGTTGGGTGCGTTGATTTAGATACGTTTATGT	9	0.17374517374517373	No Hit
ACAATAGGGATGAGATGGCCTTAAATGAATTGACGGGTAC	8	0.15444015444015444	No Hit
ACAATAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT	8	0.15444015444015444	No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATCTGCT	7	0.13513513513513514	No Hit
ACAATAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT	7	0.13513513513513514	No Hit
ACAATAGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCG	7	0.13513513513513514	No Hit
ACAATAGGGTACCTGGTTCATCCTGCCCGTAGCCTATGCT	7	0.13513513513513514	No Hit
ACAATAGGGTACCTGGTTGATCCTGCCCGTCGCCTCTGCT	6	0.11583011583011582	No Hit
ACAATAGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCC	6	0.11583011583011582	No Hit
ACAATAGGGTACCTGGTTCATCCTGCCAGTAGCATATGCT	6	0.11583011583011582	No Hit
ACAATAGGGTACCTGGTTGATCCTGCCCGTAGCATCTGCT	6	0.11583011583011582	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.019305019305019305	0.0	0.0	0.0	0.0
7	0.019305019305019305	0.0	0.0	0.0	0.0
8	0.019305019305019305	0.0	0.0	0.0	0.0
9	0.019305019305019305	0.0	0.0	0.0	0.0
10	0.019305019305019305	0.0	0.0	0.0	0.0
11	0.03861003861003861	0.0	0.0	0.0	0.0
12	0.03861003861003861	0.0	0.0	0.0	0.0
13	0.03861003861003861	0.0	0.0	0.0	0.0
14	0.03861003861003861	0.0	0.0	0.0	0.0
15	0.03861003861003861	0.0	0.0	0.0	0.0
16	0.03861003861003861	0.0	0.0	0.0	0.0
17	0.03861003861003861	0.0	0.0	0.0	0.0
18	0.03861003861003861	0.0	0.0	0.0	0.0
19	0.03861003861003861	0.0	0.0	0.0	0.0
20	0.03861003861003861	0.0	0.0	0.0	0.0
21	0.03861003861003861	0.0	0.0	0.0	0.0
22	0.03861003861003861	0.0	0.0	0.0	0.0
23	0.05791505791505792	0.0	0.0	0.0	0.0
24	0.05791505791505792	0.0	0.0	0.0	0.0
25	0.05791505791505792	0.0	0.0	0.0	0.0
26	0.05791505791505792	0.0	0.0	0.0	0.0
27	0.07722007722007722	0.0	0.0	0.0	0.0
28	0.07722007722007722	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCTGC	25	1.6814559E-4	34.264706	20
CCTGCCC	25	1.6814559E-4	34.264706	22
TGCCCGT	25	1.6814559E-4	34.264706	24
CCCGTAG	25	1.6814559E-4	34.264706	26
CTGCCCG	25	1.6814559E-4	34.264706	23
GCCCGTA	25	1.6814559E-4	34.264706	25
TCCTGCC	25	1.6814559E-4	34.264706	21
ATAGGGG	175	0.0	33.93204	4
TAGGGGA	35	8.517836E-7	33.93204	5
TAGGGCA	35	8.517836E-7	33.93204	5
GTTGATC	20	0.0025653073	33.932037	16
AGGGGGG	50	2.6921043E-10	33.932037	6
AGGGGCT	25	1.7832794E-4	33.932037	6
TGGTTGA	20	0.0025653073	33.932037	14
AGGGGAC	20	0.0025653073	33.932037	6
GGATGAG	30	1.2351124E-5	33.932037	8
GGTTGAT	20	0.0025653073	33.932037	15
GTACCTG	25	1.7832794E-4	33.932037	9
GGGTACC	25	1.7832794E-4	33.932037	7
ATAGGGT	75	0.0	33.932037	4
>>END_MODULE