FastQCFastQC Report
Fri 17 Jun 2016
SRR1525995_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525995_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5759
Sequences flagged as poor quality0
Sequence length40
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT1452.517798228859177No Hit
ACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC510.8855704115297794No Hit
ACAATAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT500.8682062858135093No Hit
ACAATAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG460.7987497829484285No Hit
ACAATAGGGACTAATCCTAGCCCTAGCCCTACACAAATAT220.3820107657579441No Hit
ACAATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG210.3646466400416739No Hit
ACAATAGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT160.27782601146032293No Hit
ACAATAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.2257336343115124No Hit
ACAATAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.20836950859524223No Hit
ACAATAGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT110.19100538287897206No Hit
ACAATAGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG100.17364125716270187No Hit
ACAATAGGGATTCGTAGGAGTAAACATAACATTCTTCCCT80.13891300573016147No Hit
ACAATAGGGGAACGTCTGCCCTATCAACTTTCGATGGTAG80.13891300573016147No Hit
ACAATAGGGATGCCATCTACCTTCTTCAACCTCACCATAG70.1215488800138913No Hit
ACAATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG70.1215488800138913No Hit
ACAATAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGC70.1215488800138913No Hit
ACAATAGGGAACATTTCCTGGGCCTTTCAGGAATACCACG60.10418475429762111No Hit
ACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGC60.10418475429762111No Hit
ACAATAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA60.10418475429762111No Hit
ACAATAGGGAGTGAAACTGCGAATGGCTCATTAAATCAGT60.10418475429762111No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG601.8189894E-1234.0000046
AGGGGAT301.2442399E-534.0000046
ATAGGGC301.2442399E-534.0000044
ATAGGGA1200.034.0000044
TAGGGGA601.8189894E-1234.0000045
TAGGGAG301.2442399E-534.0000045
TAGGGAA301.2442399E-534.0000045
AGGGGTG200.002565591434.06
AGGGGTC200.002565591434.06
AGGGGTA200.002565591434.06
ATCCTCT251.7890027E-434.016
TCCTCTT251.7890027E-434.017
GTTGATC358.6259206E-734.016
AGGGGGA405.956099E-834.06
TGGTTGA405.956099E-834.014
GGTTGAT405.956099E-834.015
CTCTTCT251.7890027E-434.019
AGGGTAC358.6259206E-734.06
TTCTGAA200.002565591434.022
AGGGATT200.002565591434.06
Produced by FastQC (version 0.11.5)