##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525995_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5759
Sequences flagged as poor quality	0
Sequence length	40
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	18.80968918214968	25.0	4.0	30.0	4.0	33.0
2	13.381142559472131	4.0	4.0	23.0	4.0	31.0
3	13.461364820281299	4.0	4.0	23.0	4.0	31.0
4	12.255079006772009	4.0	4.0	20.0	4.0	30.0
5	12.61382184407015	4.0	4.0	22.0	4.0	31.0
6	12.188748046535856	4.0	4.0	20.0	4.0	31.0
7	13.565375933321757	4.0	4.0	24.0	4.0	33.0
8	14.251085257857268	4.0	4.0	26.0	4.0	33.0
9	13.753255773571802	4.0	4.0	26.0	4.0	33.0
10	12.995832609828096	4.0	4.0	24.0	4.0	33.0
11	13.556172946692135	4.0	4.0	26.0	4.0	35.0
12	12.864733460670255	4.0	4.0	24.0	4.0	33.0
13	11.9138739364473	4.0	4.0	21.0	4.0	32.0
14	12.534815072061122	4.0	4.0	23.0	4.0	33.0
15	12.455461017537766	4.0	4.0	24.0	4.0	33.0
16	10.6233721132141	4.0	4.0	19.0	4.0	30.0
17	9.946865775308213	4.0	4.0	15.0	4.0	28.0
18	9.532384094460843	4.0	4.0	12.0	4.0	27.0
19	9.527522139260288	4.0	4.0	12.0	4.0	28.0
20	10.0526133009203	4.0	4.0	14.0	4.0	30.0
21	8.740059038027436	4.0	4.0	9.0	4.0	26.0
22	8.273311338774093	4.0	4.0	8.0	4.0	23.0
23	8.47039416565376	4.0	4.0	8.0	4.0	25.0
24	8.407362389303698	4.0	4.0	5.0	4.0	24.0
25	8.658447647160965	4.0	4.0	4.0	4.0	26.0
26	8.673207154019796	4.0	4.0	4.0	4.0	26.0
27	8.825316895294321	4.0	4.0	4.0	4.0	27.0
28	9.132314637957979	4.0	4.0	4.0	4.0	28.0
29	8.304045841291892	4.0	4.0	4.0	4.0	24.0
30	8.50199687445737	4.0	4.0	4.0	4.0	26.0
31	9.36829310644209	4.0	4.0	4.0	4.0	30.0
32	9.273658621288417	4.0	4.0	4.0	4.0	30.0
33	9.155929848932105	4.0	4.0	4.0	4.0	30.0
34	9.278173293974648	4.0	4.0	4.0	4.0	30.0
35	9.148984198645598	4.0	4.0	4.0	4.0	30.0
36	9.050008682062858	4.0	4.0	4.0	4.0	31.0
37	8.933321757249523	4.0	4.0	4.0	4.0	31.0
38	8.691613127279041	4.0	4.0	4.0	4.0	30.0
39	8.480291717312033	4.0	4.0	4.0	4.0	30.0
40	8.664872373675985	4.0	4.0	4.0	4.0	31.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	7.213198148985416
1	2	-5.3000033771632715
1	3	-8.085087641998378
1	4	-4.191535870336104
1	5	-3.7007011784074724
1	6	-3.39249933449271
1	7	-4.6126247259181685
1	8	-7.681262195857138
1	9	-4.828610322427441
1	10	-3.927303725074931
1	11	-6.086195444783346
1	12	-3.6799823457228493
1	13	-3.1295474681644517
1	14	-4.1882546156876
1	15	-2.981786570211179
1	16	-2.59252462847631
1	17	-4.635426920716226
1	18	-4.856912926660355
1	19	-4.572643938118686
1	20	-5.091826500593651
1	21	-4.019952397257333
1	22	-2.91525226248202
1	23	-3.6058258249356907
1	24	-3.683100675755065
1	25	-3.817412486865509
1	26	-3.8867340964834947
1	27	-4.078183827404285
1	28	-4.165852401214321
1	29	-3.602205414962083
1	30	-4.045809817607296
1	31	-4.311036523157789
1	32	-4.001485693370814
1	33	-3.9494928630331962
1	34	-4.113470408386384
1	35	-3.6642321361810692
1	36	-3.944179614744983
1	37	-4.035560716264339
1	38	-3.601983558680809
1	39	-3.723769409577704
1	40	-3.548250130661007
2	1	3.379864815652084
2	2	-2.07778115494105
2	3	-1.9184209753317099
2	4	-1.8026469814472144
2	5	-1.978478956185251
2	6	-4.170277112270488
2	7	-0.8904025036959471
2	8	-1.7923733069682495
2	9	-7.273054766871885
2	10	-2.427303725074932
2	11	-4.919528778116679
2	12	-3.7355379012784047
2	13	-2.2406585792755624
2	14	-2.1882546156875993
2	15	0.4071023186777101
2	16	-3.3147468506985325
2	17	-1.6354269207162258
2	18	-1.523579593327022
2	19	0.26068939521464785
2	20	0.5748401660730149
2	21	3.813380936076001
2	22	2.58474773751798
2	23	2.5608408417309754
2	24	2.316899324244935
2	25	0.8492541798011572
2	26	4.279932570183171
2	27	3.9218161725957152
2	28	3.1674809321190125
2	29	3.064461251704583
2	30	4.12085684905937
2	31	4.355630143508877
2	32	3.6651809732958522
2	33	-0.44949286303319624
2	34	2.5531962582802823
2	35	3.1691011971522647
2	36	4.555820385255017
2	37	2.6311059504023273
2	38	4.398016441319191
2	39	1.6095639237556298
2	40	2.785083202672327
3	1	7.079864815652083
3	2	4.455552178392283
3	3	5.248245691334956
3	4	6.364019685219452
3	5	3.2548543771480833
3	6	7.1630562210628455
3	7	-2.2570691703626125
3	8	7.5409600263650844
3	9	9.926945233128114
3	10	-2.793970391741598
3	11	1.7138045552166545
3	12	1.897795432054929
3	13	3.826008087391104
3	14	-0.5215879490209332
3	15	0.7737689853443754
3	16	5.518586482634801
3	17	10.364573079283774
3	18	4.943087073339646
3	19	9.227356061881315
3	20	4.908173499406349
3	21	5.1800476027426665
3	22	5.284747737517979
3	23	3.994174175064309
3	24	4.7168993242449355
3	25	5.3825875131344905
3	26	6.713265903516505
3	27	8.921816172595715
3	28	7.034147598785678
3	29	7.997794585037917
3	30	3.954190182392704
3	31	12.688963476842211
3	32	6.998514306629186
3	33	13.050507136966804
3	34	10.486529591613618
3	35	11.13576786381893
3	36	9.055820385255018
3	37	10.764439283735662
3	38	10.398016441319191
3	39	9.876230590422297
3	40	14.251749869338994
4	1	3.879864815652084
4	2	8.255552178392284
4	3	-1.7517543086650438
4	4	8.364019685219452
4	5	11.854854377148083
4	6	-0.8369437789371545
4	7	10.942930829637387
4	8	5.5409600263650844
4	9	9.726945233128115
4	10	-0.5939703917415979
4	11	23.913804555216654
4	12	20.09779543205493
4	13	20.426008087391104
4	14	20.478412050979067
4	15	25.573768985344376
4	16	28.518586482634802
4	17	20.364573079283772
4	18	29.143087073339643
4	19	29.427356061881312
4	20	28.90817349940635
4	21	27.980047602742665
4	22	26.08474773751798
4	23	28.394174175064308
4	24	22.316899324244936
4	25	28.182587513134493
4	26	27.113265903516506
4	27	26.921816172595715
4	28	26.834147598785677
4	29	19.397794585037918
4	30	21.954190182392704
4	31	25.68896347684221
4	32	20.998514306629186
4	33	28.050507136966804
4	34	25.886529591613616
4	35	28.33576786381893
4	36	27.055820385255018
4	37	24.96443928373566
4	38	18.398016441319193
4	39	27.276230590422294
4	40	25.451749869338993
5	1	-2.120135184347916
5	2	3.255552178392284
5	3	-1.9739765308872652
5	4	-4.413758092558326
5	5	0.854854377148083
5	6	-2.8369437789371545
5	7	-0.3904025036959471
5	8	0.6520711374761952
5	9	1.7269452331281148
5	10	0.9615851638139574
5	11	1.4693601107722092
5	12	2.0977954320549284
5	13	-0.35176969038667494
5	14	-0.5215879490209332
5	15	2.4626578742332654
5	16	4.40747537152369
5	17	3.142350857061553
5	18	0.6986426288952003
5	19	3.5384671729924246
5	20	3.463729054961904
5	21	3.757825380520446
5	22	4.529192181962425
5	23	6.171951952842088
5	24	5.650232657578269
5	25	3.4048097353567126
5	26	3.55771034796095
5	27	4.588482839262381
5	28	3.278592043230123
5	29	2.5089056961490277
5	30	4.398634626837149
5	31	7.022296810175545
5	32	6.109625417740297
5	33	2.1616182480779145
5	34	4.330974036058061
5	35	5.335767863818931
5	36	4.611375940810572
5	37	5.408883728180106
5	38	-0.15753911423636424
5	39	-0.8348805206888148
5	40	-0.7704723528832291
6	1	0.8798648156520841
6	2	10.255552178392284
6	3	20.248245691334958
6	4	8.364019685219452
6	5	25.854854377148083
6	6	23.163056221062845
6	7	12.942930829637387
6	8	15.540960026365084
6	9	16.726945233128113
6	10	19.4060296082584
6	11	17.913804555216654
6	12	-6.902204567945072
6	13	-6.573991912608896
6	14	-6.521587949020933
6	15	-6.426231014655624
6	16	-5.481413517365199
6	17	-4.635426920716226
6	18	-4.856912926660355
6	19	-4.572643938118686
6	20	-5.091826500593651
6	21	-4.019952397257333
6	22	-2.91525226248202
6	23	-3.6058258249356907
6	24	-3.683100675755065
6	25	-3.817412486865509
6	26	-3.8867340964834947
6	27	-4.078183827404285
6	28	-4.165852401214321
6	29	-3.602205414962083
6	30	-4.045809817607296
6	31	-4.311036523157789
6	32	-4.001485693370814
6	33	-3.9494928630331962
6	34	-4.113470408386384
6	35	-3.6642321361810692
6	36	-3.944179614744983
6	37	-4.035560716264339
6	38	-3.601983558680809
6	39	-3.723769409577704
6	40	-3.548250130661007
8	1	0.6798648156520848
8	2	3.988885511725618
8	3	-1.3517543086650434
8	4	-2.769313648113881
8	5	2.0548543771480823
8	6	-2.3036104456038213
8	7	-3.52373583702928
8	8	-3.525706640301582
8	9	-2.1397214335385524
8	10	-1.460637058408265
8	11	-6.286195444783346
8	12	-4.768871234611738
8	13	-3.3073252459422298
8	14	-3.5882546156875996
8	15	-2.4928976813222903
8	16	-2.4814135173651994
8	17	-2.035426920716226
8	18	-1.2569129266603554
8	19	-2.239310604785353
8	20	-1.4918265005936515
8	21	-2.419952397257333
8	22	0.4847477375179805
8	23	-1.2724924916023577
8	24	-3.083100675755065
8	25	-2.2840791535321756
8	26	0.7799325701831714
8	27	-0.2115171607376185
8	28	0.30081426545234535
8	29	-1.602205414962083
8	30	-0.5791431509406291
8	31	0.8222968101755441
8	32	1.5318476399625194
8	33	0.3838404703001377
8	34	0.9531962582802826
8	35	1.53576786381893
8	36	0.9224870519216841
8	37	0.6977726170689937
8	38	-0.0019835586808092387
8	39	2.142897257088963
8	40	2.3850832026723268
9	1	4.004864815652084
9	2	14.005552178392284
9	3	6.998245691334956
9	4	7.864019685219452
9	5	7.604854377148083
9	6	4.2880562210628455
9	7	8.192930829637387
9	8	10.540960026365084
9	9	8.726945233128115
9	10	7.656029608258402
9	11	5.413804555216654
9	12	7.097795432054928
9	13	4.801008087391104
9	14	3.728412050979067
9	15	8.198768985344376
9	16	6.018586482634801
9	17	4.864573079283774
9	18	2.143087073339645
9	19	6.302356061881314
9	20	6.158173499406349
9	21	5.730047602742667
9	22	8.83474773751798
9	23	6.769174175064309
9	24	4.316899324244935
9	25	7.807587513134491
9	26	9.113265903516506
9	27	8.796816172595715
9	28	7.459147598785679
9	29	5.647794585037917
9	30	6.329190182392704
9	31	8.688963476842211
9	32	6.748514306629186
9	33	6.800507136966804
9	34	7.261529591613616
9	35	3.7107678638189308
9	36	7.930820385255017
9	37	6.964439283735661
9	38	6.773016441319191
9	39	3.901230590422296
9	40	5.076749869338993
10	1	7.879864815652084
10	2	5.755552178392284
10	3	1.7482456913349562
10	4	5.864019685219452
10	5	-0.145145622851917
10	6	2.1630562210628455
10	7	10.442930829637387
10	8	10.040960026365084
10	9	14.226945233128115
10	10	3.406029608258402
10	11	10.913804555216654
10	12	16.09779543205493
10	13	17.926008087391104
10	14	9.978412050979067
10	15	11.073768985344376
10	16	9.0185864826348
10	17	7.864573079283774
10	18	20.143087073339643
10	19	13.927356061881314
10	20	20.90817349940635
10	21	17.480047602742665
10	22	1.0847477375179801
10	23	7.394174175064309
10	24	7.316899324244935
10	25	16.182587513134493
10	26	10.113265903516506
10	27	22.921816172595715
10	28	18.834147598785677
10	29	20.897794585037918
10	30	19.954190182392704
10	31	10.688963476842211
10	32	23.998514306629186
10	33	20.550507136966804
10	34	17.886529591613616
10	35	-2.6642321361810692
10	36	1.555820385255017
10	37	4.464439283735661
10	38	9.898016441319191
10	39	3.776230590422296
10	40	9.451749869338993
12	1	3.879864815652084
12	2	16.255552178392286
12	3	7.248245691334956
12	4	3.3640196852194517
12	5	7.854854377148083
12	6	6.1630562210628455
12	7	7.942930829637387
12	8	14.540960026365084
12	9	10.726945233128115
12	10	9.406029608258402
12	11	14.913804555216654
12	12	24.09779543205493
12	13	16.426008087391104
12	14	22.478412050979067
12	15	22.573768985344376
12	16	7.518586482634801
12	17	1.3645730792837742
12	18	17.143087073339643
12	19	1.427356061881314
12	20	20.90817349940635
12	21	9.980047602742667
12	22	8.08474773751798
12	23	12.39417417506431
12	24	19.316899324244936
12	25	5.182587513134491
12	26	18.113265903516506
12	27	12.921816172595715
12	28	18.834147598785677
12	29	8.397794585037918
12	30	22.954190182392704
12	31	26.68896347684221
12	32	16.998514306629186
12	33	27.050507136966804
12	34	16.886529591613616
12	35	18.33576786381893
12	36	21.055820385255018
12	37	26.96443928373566
12	38	10.398016441319191
12	39	27.276230590422294
12	40	21.451749869338993
13	1	12.213198148985416
13	2	6.922218845058952
13	3	4.248245691334956
13	4	7.03068635188612
13	5	-0.145145622851917
13	6	6.1630562210628455
13	7	9.609597496304055
13	8	12.207626693031752
13	9	11.393611899794783
13	10	12.07269627492507
13	11	22.580471221883318
13	12	17.09779543205493
13	13	24.75934142072444
13	14	15.145078717645735
13	15	21.573768985344376
13	16	17.185253149301467
13	17	4.364573079283774
13	18	11.476420406672977
13	19	9.427356061881314
13	20	9.241506832739681
13	21	5.313380936076001
13	22	1.418081070851314
13	23	9.060840841730977
13	24	5.316899324244935
13	25	5.182587513134491
13	26	-0.5534007631501616
13	27	4.588482839262381
13	28	3.5008142654523446
13	29	-1.26887208162875
13	30	1.9541901823927041
13	31	1.3556301435088773
13	32	3.6651809732958522
13	33	5.71717380363347
13	34	4.886529591613616
13	35	0.6691011971522647
13	36	1.389153718588351
13	37	0.9644392837356612
13	38	5.398016441319191
13	39	5.942897257088962
13	40	1.1184165360056593
14	1	13.879864815652084
14	2	12.255552178392284
14	3	9.248245691334956
14	4	19.364019685219453
14	5	12.854854377148083
14	6	19.163056221062845
14	7	20.942930829637387
14	8	20.540960026365084
14	9	20.726945233128113
14	10	13.406029608258402
14	11	19.913804555216654
14	12	18.09779543205493
14	13	21.426008087391104
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106	15	7.773768985344375
106	16	5.318586482634801
106	17	10.164573079283775
106	18	8.943087073339646
106	19	8.027356061881315
106	20	10.908173499406349
106	21	-4.019952397257333
106	22	-2.91525226248202
106	23	-3.6058258249356907
106	24	-3.683100675755065
106	25	-3.817412486865509
106	26	-3.8867340964834947
106	27	-4.078183827404285
106	28	-4.165852401214321
106	29	-3.602205414962083
106	30	-4.045809817607296
106	31	-4.311036523157789
106	32	-4.001485693370814
106	33	-3.9494928630331962
106	34	-4.113470408386384
106	35	-3.6642321361810692
106	36	-3.944179614744983
106	37	-4.035560716264339
106	38	-3.601983558680809
106	39	-3.723769409577704
106	40	-3.548250130661007
107	1	7.070341006128274
107	2	6.731742654582758
107	3	7.533959977049241
107	4	3.840210161409928
107	5	6.140568662862368
107	6	2.5916276496342743
107	7	4.609597496304055
107	8	4.493340978746037
107	9	4.345992852175735
107	10	2.7869819892107834
107	11	0.7709474123595115
107	12	2.145414479673976
107	13	1.90219856358158
107	14	2.5260310985981143
107	15	3.0499594615348524
107	16	0.3757293397776582
107	17	1.9360016507123454
107	18	0.3335632638158348
107	19	-0.3821677476424963
107	20	1.0986496898825386
107	21	-0.2580476353525709
107	22	0.7514144041846471
107	23	-1.8915401106499763
107	24	-1.4450054376598267
107	25	-2.0078886773416995
107	26	-0.8867340964834947
107	27	-0.221040970261428
107	28	-0.16585240121432143
107	29	0.25493744218077374
107	30	-0.42676219855967723
107	31	-0.453893666014932
107	32	0.5699428780577573
107	33	0.43145951791918513
107	34	-0.39918469410066937
107	35	-0.8070892790382125
107	36	-0.37275104331641185
107	37	0.15491547421185103
107	38	-0.6972216539189038
107	39	-0.6761503619586566
107	40	-0.786345368756245
108	1	10.1980466338339
108	2	7.801006723846831
108	3	6.657336600425866
108	4	7.364019685219452
108	5	7.854854377148083
108	6	4.890328948335572
108	7	9.71565810236466
108	8	9.404596390001448
108	9	8.772399778582662
108	10	10.587847790076585
108	11	4.504713646125744
108	12	3.27961361387311
108	13	2.5623717237547403
108	14	4.387502960069975
108	15	1.7555871671625578
108	16	-0.02686806281974441
108	17	0.27366398837468253
108	18	1.5067234369760083
108	19	0.10917424369949558
108	20	-0.6372810460481961
108	21	-0.7926796699846053
108	22	0.4029295556997985
108	23	-1.8785530976629632
108	24	-1.5467370393914281
108	25	-0.9992306686836905
108	26	-0.6140068237562222
108	27	-0.8054565546770123
108	28	-1.2567614921234123
108	29	0.2614309486742803
108	30	-1.954900726698205
108	31	-0.9474001595214254
108	32	-0.7742129660980863
108	33	0.09596168242134873
108	34	-0.3407431356591113
108	35	-0.30059577254470593
108	36	0.3740022034368353
108	37	-0.21737889808252042
108	38	0.48892553222828283
108	39	0.5944124086041143
108	40	-0.502795585206461
109	1	-7.870135184347916
109	2	-5.244447821607716
109	3	-3.376754308665044
109	4	-4.510980314780548
109	5	-5.895145622851917
109	6	-2.9619437789371545
109	7	-4.432069170362613
109	8	-4.8340399736349156
109	9	-4.523054766871885
109	10	-2.468970391741598
109	11	-1.5861954447833462
109	12	-2.6522045679450716
109	13	-3.4489919126088964
109	14	-2.646587949020933
109	15	0.44876898534437615
109	16	-5.481413517365199
109	17	-4.635426920716226
109	18	-4.856912926660355
109	19	-4.572643938118686
109	20	-5.091826500593651
109	21	-4.019952397257333
109	22	-2.91525226248202
109	23	-3.6058258249356907
109	24	-3.683100675755065
109	25	-3.817412486865509
109	26	-3.8867340964834947
109	27	-4.078183827404285
109	28	-4.165852401214321
109	29	-3.602205414962083
109	30	-4.045809817607296
109	31	-4.311036523157789
109	32	-4.001485693370814
109	33	-3.9494928630331962
109	34	-4.113470408386384
109	35	-3.6642321361810692
109	36	-3.944179614744983
109	37	-4.035560716264339
109	38	-3.601983558680809
109	39	-3.723769409577704
109	40	-3.548250130661007
110	1	-14.120135184347916
110	2	-8.744447821607716
110	3	-8.751754308665044
110	4	-7.635980314780548
110	5	-7.145145622851917
110	6	-6.8369437789371545
110	7	-8.057069170362613
110	8	-8.459039973634916
110	9	-8.273054766871885
110	10	-6.593970391741598
110	11	-8.086195444783346
110	12	-6.902204567945072
110	13	-6.573991912608896
110	14	-6.521587949020933
110	15	-6.426231014655624
110	16	-5.481413517365199
110	17	-4.635426920716226
110	18	-4.856912926660355
110	19	-4.572643938118686
110	20	-5.091826500593651
110	21	-4.019952397257333
110	22	-2.91525226248202
110	23	-3.6058258249356907
110	24	-3.683100675755065
110	25	-3.817412486865509
110	26	-3.8867340964834947
110	27	-4.078183827404285
110	28	-4.165852401214321
110	29	-3.602205414962083
110	30	-4.045809817607296
110	31	-4.311036523157789
110	32	-4.001485693370814
110	33	-3.9494928630331962
110	34	-4.113470408386384
110	35	-3.6642321361810692
110	36	-3.944179614744983
110	37	-4.035560716264339
110	38	-3.601983558680809
110	39	-3.723769409577704
110	40	-3.548250130661007
111	1	0.7548648156520841
111	2	3.755552178392284
111	3	4.498245691334956
111	4	5.489019685219452
111	5	-2.770145622851917
111	6	9.413056221062845
111	7	9.442930829637387
111	8	9.165960026365084
111	9	5.726945233128115
111	10	5.531029608258402
111	11	4.163804555216654
111	12	6.097795432054928
111	13	6.676008087391104
111	14	8.103412050979067
111	15	7.323768985344376
111	16	-0.8564135173651994
111	17	-0.010426920716225752
111	18	0.893087073339645
111	19	2.552356061881314
111	20	2.033173499406349
111	21	-3.2699523972573328
111	22	-1.2902522624820199
111	23	-1.2308258249356907
111	24	-2.183100675755065
111	25	-1.4424124868655088
111	26	-2.8867340964834947
111	27	-1.8281838274042848
111	28	-2.4158524012143214
111	29	-2.227205414962083
111	30	-2.170809817607296
111	31	-3.4360365231577887
111	32	-1.1264856933708138
111	33	-0.32449286303319624
111	34	-0.7384704083863838
111	35	-1.0392321361810692
111	36	-1.694179614744983
111	37	-0.9105607162643388
111	38	0.2730164413191911
111	39	-0.9737694095777041
111	40	0.3267498693389932
112	1	8.213198148985416
112	2	5.255552178392284
112	3	13.248245691334956
112	4	15.364019685219452
112	5	9.854854377148083
112	6	16.496389554396178
112	7	19.942930829637387
112	8	18.874293359698417
112	9	18.393611899794784
112	10	19.07269627492507
112	11	1.58047122188332
112	12	2.0977954320549284
112	13	2.4260080873911036
112	14	-0.5215879490209332
112	15	2.2404356520110422
112	16	4.185253149301467
112	17	2.3645730792837742
112	18	-2.856912926660355
112	19	1.09402272854798
112	20	4.574840166073015
112	21	-0.3532857305906658
112	22	2.418081070851314
112	23	1.7275075083976432
112	24	1.3168993242449352
112	25	0.5159208464678251
112	26	-0.5534007631501616
112	27	0.5884828392623813
112	28	-2.1658524012143214
112	29	-1.602205414962083
112	30	1.6208568490593702
112	31	-0.31103652315778874
112	32	0.6651809732958522
112	33	-0.2828261963665293
112	34	-1.1134704083863838
112	35	2.3357678638189308
112	36	5.722487051921683
112	37	4.631105950402327
112	38	1.731349774652525
112	39	5.942897257088962
112	40	1.7850832026723271
114	1	-14.120135184347916
114	2	-8.744447821607716
114	3	-8.751754308665044
114	4	-7.635980314780548
114	5	-7.145145622851917
114	6	-6.8369437789371545
114	7	-8.057069170362613
114	8	-8.459039973634916
114	9	-8.273054766871885
114	10	-6.593970391741598
114	11	-8.086195444783346
114	12	-6.902204567945072
114	13	-6.573991912608896
114	14	-6.521587949020933
114	15	-6.426231014655624
114	16	-5.481413517365199
114	17	-4.635426920716226
114	18	-4.856912926660355
114	19	-4.572643938118686
114	20	-5.091826500593651
114	21	-4.019952397257333
114	22	-2.91525226248202
114	23	-3.6058258249356907
114	24	-3.683100675755065
114	25	-3.817412486865509
114	26	-3.8867340964834947
114	27	-4.078183827404285
114	28	-4.165852401214321
114	29	-3.602205414962083
114	30	-4.045809817607296
114	31	-4.311036523157789
114	32	-4.001485693370814
114	33	-3.9494928630331962
114	34	-4.113470408386384
114	35	-3.6642321361810692
114	36	-3.944179614744983
114	37	-4.035560716264339
114	38	-3.601983558680809
114	39	-3.723769409577704
114	40	-3.548250130661007
117	1	-7.620135184347916
117	2	-8.744447821607716
117	3	-8.751754308665044
117	4	-7.635980314780548
117	5	-7.145145622851917
117	6	-6.8369437789371545
117	7	-8.057069170362613
117	8	-8.459039973634916
117	9	-8.273054766871885
117	10	-6.593970391741598
117	11	-8.086195444783346
117	12	-6.902204567945072
117	13	-6.573991912608896
117	14	-6.521587949020933
117	15	-6.426231014655624
117	16	-5.481413517365199
117	17	-4.635426920716226
117	18	-4.856912926660355
117	19	-4.572643938118686
117	20	-5.091826500593651
117	21	-4.019952397257333
117	22	-2.91525226248202
117	23	-3.6058258249356907
117	24	-3.683100675755065
117	25	-3.817412486865509
117	26	-3.8867340964834947
117	27	-4.078183827404285
117	28	-4.165852401214321
117	29	-3.602205414962083
117	30	-4.045809817607296
117	31	-4.311036523157789
117	32	-4.001485693370814
117	33	-3.9494928630331962
117	34	-4.113470408386384
117	35	-3.6642321361810692
117	36	-3.944179614744983
117	37	-4.035560716264339
117	38	-3.601983558680809
117	39	-3.723769409577704
117	40	-3.548250130661007
118	1	-3.45346851768125
118	2	1.92221884505895
118	3	1.2482456913349562
118	4	-2.6359803147805483
118	5	2.188187710481417
118	6	2.1630562210628455
118	7	-8.057069170362613
118	8	-8.459039973634916
118	9	-8.273054766871885
118	10	-6.593970391741598
118	11	-8.086195444783346
118	12	-6.902204567945072
118	13	-6.573991912608896
118	14	-6.521587949020933
118	15	-6.426231014655624
118	16	-5.481413517365199
118	17	-4.635426920716226
118	18	-4.856912926660355
118	19	-4.572643938118686
118	20	-5.091826500593651
118	21	-4.019952397257333
118	22	-2.91525226248202
118	23	-3.6058258249356907
118	24	-3.683100675755065
118	25	-3.817412486865509
118	26	-3.8867340964834947
118	27	-4.078183827404285
118	28	-4.165852401214321
118	29	-3.602205414962083
118	30	-4.045809817607296
118	31	-4.311036523157789
118	32	-4.001485693370814
118	33	-3.9494928630331962
118	34	-4.113470408386384
118	35	-3.6642321361810692
118	36	-3.944179614744983
118	37	-4.035560716264339
118	38	-3.601983558680809
118	39	-3.723769409577704
118	40	-3.548250130661007
119	1	-1.120135184347916
119	2	0.25555217839228384
119	3	3.5815790246682884
119	4	-3.3026469814472144
119	5	-2.811812289518583
119	6	2.8297228877295115
119	7	4.276264162970719
119	8	1.2076266930317505
119	9	-2.606388100205219
119	10	9.406029608258402
119	11	9.580471221883322
119	12	4.431128765388262
119	13	9.759341420724436
119	14	9.811745384312399
119	15	10.907102318677708
119	16	13.5185864826348
119	17	2.0312397459504403
119	18	4.143087073339645
119	19	-3.239310604785353
119	20	4.241506832739683
119	21	-4.019952397257333
119	22	-2.91525226248202
119	23	-3.6058258249356907
119	24	-3.683100675755065
119	25	-3.817412486865509
119	26	-3.8867340964834947
119	27	-4.078183827404285
119	28	-4.165852401214321
119	29	-3.602205414962083
119	30	-4.045809817607296
119	31	-4.311036523157789
119	32	-4.001485693370814
119	33	-3.9494928630331962
119	34	-4.113470408386384
119	35	-3.6642321361810692
119	36	-3.944179614744983
119	37	-4.035560716264339
119	38	-3.601983558680809
119	39	-3.723769409577704
119	40	-3.548250130661007
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	2915.0
5	113.0
6	121.0
7	84.0
8	71.0
9	135.0
10	166.0
11	168.0
12	136.0
13	135.0
14	108.0
15	83.0
16	74.0
17	58.0
18	43.0
19	81.0
20	145.0
21	186.0
22	160.0
23	147.0
24	145.0
25	127.0
26	112.0
27	94.0
28	57.0
29	45.0
30	25.0
31	16.0
32	6.0
33	1.0
34	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	96.99025748086291	1.6701461377870561	0.8350730688935281	0.5045233124565066
8	96.78428645923866	1.7903702416130713	1.0081696506170694	0.4171736485312011
9	96.40125173852573	1.6168289290681501	1.2691237830319888	0.7127955493741307
10	44.36534120507032	35.301267581177285	13.960757075881231	6.372634137871158
11	31.290154540718873	30.578225386351797	23.493662094113564	14.637957978815766
12	30.317763500607743	25.698906060079874	24.46605313422469	19.51727730508769
13	21.57885585115632	23.456790123456788	26.586680577290906	28.377673448095987
14	20.152883947185547	31.966643502432245	31.58443363446838	16.29603891591383
15	32.0368119465185	25.95936794582393	28.025698906060082	13.9781212015975
16	31.70689355790936	30.369855877756557	23.979857614169127	13.94339295016496
17	24.900156277131448	28.268796666087866	32.627192220871684	14.203854835909013
18	23.710265763418448	28.573910022581206	30.953621677957273	16.762202536043077
19	24.374565670604586	30.59416261292564	25.0	20.03127171646977
20	22.955374196909187	32.74874110088557	29.241187706198996	15.05469699600625
21	21.44469525959368	28.70289980899462	30.283035249175207	19.5693696822365
22	19.63882618510158	27.313769751693002	30.66504601493315	22.38235804827227
23	19.138589788120875	27.83952761375478	29.767280305661686	23.25460229246266
24	19.53464143080396	30.144122243445043	29.97048098628234	20.350755339468655
25	24.396177237185057	29.12250217202433	26.88097306689835	19.600347523892268
26	24.024390243902438	29.599303135888498	23.37979094076655	22.99651567944251
27	18.219822330604423	29.83800731579864	24.560181153109216	27.381989200487723
28	20.382608695652173	34.452173913043474	24.208695652173915	20.956521739130434
29	26.908330428720813	31.125827814569533	23.42279539909376	18.543046357615893
30	22.848200312989047	27.351764910450356	32.70735524256651	17.092679533994087
31	24.630884141045684	31.804759423310752	26.281049157547333	17.28330727809623
32	28.725814885828832	28.55150775666725	24.10667596304689	18.616001394457033
33	24.591872177839527	29.40257033692254	26.189649183744358	19.81590830149357
34	20.246570585171035	31.029692654974824	32.0368119465185	16.68692481333565
35	18.041326619204725	32.47091508942525	34.0684146553221	15.419343636047925
36	20.780351855077512	33.65267375021773	26.423967949834527	19.14300644487023
37	21.097412745268276	31.44643167216531	30.856051397812116	16.600104184754297
38	25.28655783258076	30.878777353247656	27.0059048280653	16.828759986106288
39	22.364993922555996	29.484285466226773	25.5079006772009	22.642819934016323
40	20.229206459454765	29.987845111998613	31.272790415002603	18.510158013544018
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	1.5
2	3.0
3	3.0
4	3.0
5	3.0
6	6.5
7	10.0
8	10.0
9	21.5
10	33.0
11	39.0
12	45.0
13	45.0
14	45.5
15	46.0
16	52.0
17	58.0
18	58.0
19	65.0
20	72.0
21	78.0
22	84.0
23	84.0
24	100.5
25	117.0
26	124.0
27	131.0
28	131.0
29	167.5
30	204.0
31	242.5
32	281.0
33	281.0
34	293.0
35	305.0
36	366.5
37	428.0
38	428.0
39	449.0
40	470.0
41	490.5
42	511.0
43	511.0
44	506.5
45	502.0
46	584.5
47	667.0
48	667.0
49	584.5
50	502.0
51	451.5
52	401.0
53	401.0
54	372.0
55	343.0
56	325.5
57	308.0
58	308.0
59	216.0
60	124.0
61	92.0
62	60.0
63	60.0
64	46.5
65	33.0
66	20.0
67	7.0
68	7.0
69	5.5
70	4.0
71	5.5
72	7.0
73	7.0
74	3.5
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.19100538287897206
8	0.10418475429762111
9	0.1215488800138913
10	0.0
11	0.0
12	0.0
13	0.13891300573016147
14	0.052092377148810556
15	0.0
16	0.0
17	0.0
18	0.034728251432540366
19	0.052092377148810556
20	0.0
21	0.0
22	0.0
23	0.017364125716270183
24	0.0
25	0.06945650286508073
26	0.32991838860913353
27	0.31255426289286337
28	0.15627713144643168
29	0.3646466400416739
30	0.13891300573016147
31	0.034728251432540366
32	0.3820107657579441
33	0.017364125716270183
34	0.0
35	0.0
36	0.31255426289286337
37	0.0
38	0.017364125716270183
39	0.0
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	5759.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.68171557562077
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.99198717948718	82.34068414655323
2	3.3653846153846154	5.8343462406667825
3	0.6610576923076923	1.7190484459107485
4	0.4206730769230769	1.4585865601666956
5	0.16025641025641024	0.6945650286508075
6	0.08012820512820512	0.41673901719048445
7	0.06009615384615385	0.3646466400416739
8	0.04006410256410256	0.27782601146032293
9	0.0	0.0
>10	0.18028846153846154	3.490189268970308
>50	0.02003205128205128	0.8855704115297794
>100	0.02003205128205128	2.517798228859177
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT	145	2.517798228859177	No Hit
ACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC	51	0.8855704115297794	No Hit
ACAATAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT	50	0.8682062858135093	No Hit
ACAATAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG	46	0.7987497829484285	No Hit
ACAATAGGGACTAATCCTAGCCCTAGCCCTACACAAATAT	22	0.3820107657579441	No Hit
ACAATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG	21	0.3646466400416739	No Hit
ACAATAGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT	16	0.27782601146032293	No Hit
ACAATAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13	0.2257336343115124	No Hit
ACAATAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12	0.20836950859524223	No Hit
ACAATAGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT	11	0.19100538287897206	No Hit
ACAATAGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG	10	0.17364125716270187	No Hit
ACAATAGGGATTCGTAGGAGTAAACATAACATTCTTCCCT	8	0.13891300573016147	No Hit
ACAATAGGGGAACGTCTGCCCTATCAACTTTCGATGGTAG	8	0.13891300573016147	No Hit
ACAATAGGGATGCCATCTACCTTCTTCAACCTCACCATAG	7	0.1215488800138913	No Hit
ACAATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG	7	0.1215488800138913	No Hit
ACAATAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGC	7	0.1215488800138913	No Hit
ACAATAGGGAACATTTCCTGGGCCTTTCAGGAATACCACG	6	0.10418475429762111	No Hit
ACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGC	6	0.10418475429762111	No Hit
ACAATAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA	6	0.10418475429762111	No Hit
ACAATAGGGAGTGAAACTGCGAATGGCTCATTAAATCAGT	6	0.10418475429762111	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.017364125716270187	0.0	0.0	0.0	0.0
15	0.017364125716270187	0.0	0.0	0.0	0.0
16	0.017364125716270187	0.0	0.0	0.0	0.0
17	0.017364125716270187	0.0	0.0	0.0	0.0
18	0.017364125716270187	0.0	0.0	0.0	0.0
19	0.017364125716270187	0.0	0.0	0.0	0.0
20	0.017364125716270187	0.0	0.0	0.0	0.0
21	0.017364125716270187	0.0	0.0	0.0	0.0
22	0.017364125716270187	0.0	0.0	0.0	0.0
23	0.017364125716270187	0.0	0.0	0.0	0.0
24	0.017364125716270187	0.0	0.0	0.0	0.0
25	0.017364125716270187	0.0	0.0	0.0	0.0
26	0.017364125716270187	0.0	0.0	0.0	0.0
27	0.017364125716270187	0.0	0.0	0.0	0.0
28	0.017364125716270187	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGGGGG	60	1.8189894E-12	34.000004	6
AGGGGAT	30	1.2442399E-5	34.000004	6
ATAGGGC	30	1.2442399E-5	34.000004	4
ATAGGGA	120	0.0	34.000004	4
TAGGGGA	60	1.8189894E-12	34.000004	5
TAGGGAG	30	1.2442399E-5	34.000004	5
TAGGGAA	30	1.2442399E-5	34.000004	5
AGGGGTG	20	0.0025655914	34.0	6
AGGGGTC	20	0.0025655914	34.0	6
AGGGGTA	20	0.0025655914	34.0	6
ATCCTCT	25	1.7890027E-4	34.0	16
TCCTCTT	25	1.7890027E-4	34.0	17
GTTGATC	35	8.6259206E-7	34.0	16
AGGGGGA	40	5.956099E-8	34.0	6
TGGTTGA	40	5.956099E-8	34.0	14
GGTTGAT	40	5.956099E-8	34.0	15
CTCTTCT	25	1.7890027E-4	34.0	19
AGGGTAC	35	8.6259206E-7	34.0	6
TTCTGAA	20	0.0025655914	34.0	22
AGGGATT	20	0.0025655914	34.0	6
>>END_MODULE