FastQCFastQC Report
Fri 17 Jun 2016
SRR1525994_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525994_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62482
Sequences flagged as poor quality0
Sequence length56
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGAGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT2990.4785378188918409No Hit
AGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC2370.3793092410614257No Hit
AGCGAGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC1950.31208988188598313No Hit
AGCGAGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT1430.22886591338305431No Hit
AGCGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG1300.2080599212573221No Hit
AGCGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1100.17605070260234948No Hit
AGCGAGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC980.1568451714093659No Hit
AGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC970.15524471047661728No Hit
AGCGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC930.14884286674562275No Hit
AGCGAGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT740.11843410902339874No Hit
AGCGAGGGGACTAATCCTAGCCCTAGCCCTACACAAATATCATTATACTATTATAT740.11843410902339874No Hit
AGCGAGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC680.10883134342690695No Hit
AGCGAGGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCTAGC660.10563042156140967No Hit
AGCGAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTG630.10082903876316379No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGAC203.9476593E-450.5162441
TCTTTCA203.9635212E-450.47526638
CTTTCAC203.9635212E-450.47526639
TATCACA150.00808539250.4752639
TTATCAC150.00808539250.4752638
GCCGATT150.00808539250.4752638
CGGATTG451.200533E-1050.4752639
GCGGATT451.200533E-1050.4752638
GGCGGAT451.2187229E-1050.4343637
TCGGACG309.774049E-750.3935236
AATACAA150.00813741850.3935236
CCATTCT150.00813741850.3935236
ATCGGAC309.828891E-750.35275335
TTATCTC150.00829498950.1498847
ATTATAC301.0107051E-650.1498842
TTATACT301.0163476E-650.109543
TATTATA301.0277145E-650.0289449
CTATTAT301.0277145E-650.0289448
AGAGGTG150.00837462150.0289415
GACAGTT150.00840129149.9887589
Produced by FastQC (version 0.11.5)