FastQCFastQC Report
Fri 17 Jun 2016
SRR1525993_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525993_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2883
Sequences flagged as poor quality0
Sequence length40
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGAGGGGGGGGCTGGAGAGATGGCTCCGTGGTTAGGAG70.2428026361429067No Hit
AGCGAGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAGGAG70.2428026361429067No Hit
AGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC70.2428026361429067No Hit
AGCGAGGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC50.1734304543877905No Hit
AGCGAGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT40.1387443635102324No Hit
AGCGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT40.1387443635102324No Hit
AGCGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG40.1387443635102324No Hit
AGCGAGGGGTACCTGGTTGATCCTGCCCGTAGCATATGCT40.1387443635102324No Hit
AGCGAGGGGGTGTACGAGTTTTTCTTACGTTTCTTGGAGT40.1387443635102324No Hit
AGCGAGGGGTGCAGAGTCTGAGGTGCAAGTTAGCAAGGCT40.1387443635102324No Hit
AGCGAGGGGTGAGATGGACTTAAAGGAATTGACGGGTACG40.1387443635102324No Hit
AGCGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT30.10405827263267431No Hit
AGCGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC30.10405827263267431No Hit
AGCGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG30.10405827263267431No Hit
AGCGAGGGGATTTCAGGCTCTATGTAAGGACAATATCTAC30.10405827263267431No Hit
AGCGAGGGGTGAGATGGACTCAAATGAATTGACGGGTACG30.10405827263267431No Hit
AGCGAGGGGACGTGTGTGTTCTTGTTCCTGTCAGCAGCAC30.10405827263267431No Hit
AGCGAGGGGTGAGATGGACTTAAATGAATTGACGGGTACG30.10405827263267431No Hit
AGCGAGGGGATTTCAGGCCCTATGTAAGGACAATATCTAC30.10405827263267431No Hit
AGCGAGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAG30.10405827263267431No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGGGA1150.034.4821474
AGGGGGT501.4188117E-1034.4821435
AGGGGGG355.9452395E-734.4821435
AGGGGGA309.299525E-634.4821435
AGGGGAT355.9452395E-734.4821435
AGGGGAC309.299525E-634.4821435
AGGGGAA501.4188117E-1034.4821435
CGAGGGG2750.034.4821433
GGGGGTC200.002185905834.4821436
GAGGGGC251.4348784E-434.4821434
GGGGAAG200.002185905834.4821436
AGCGAGG2800.034.4821431
GCGAGGG2750.034.4821432
GAGGGGG1300.033.1559074
GGGGGGG500.008218789517.2410726
Produced by FastQC (version 0.11.5)