FastQCFastQC Report
Fri 17 Jun 2016
SRR1525991_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525991_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2716
Sequences flagged as poor quality0
Sequence length40
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC351.2886597938144329No Hit
AGCGAGGGGACTAATCCTAGCCCTAGCCCTACACAAATAT140.5154639175257731No Hit
AGCGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT130.4786450662739323No Hit
AGCGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG110.40500736377025043No Hit
AGCGAGGGGATGCCACAACTAGATACATCAACATGATTTA100.3681885125184094No Hit
AGCGAGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT100.3681885125184094No Hit
AGCGAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT90.33136966126656847No Hit
AGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGC80.29455081001472755No Hit
AGCGAGGGGATAACTTGAGTTTTTTTCAACATGTGTGTTA60.22091310751104565No Hit
AGCGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG60.22091310751104565No Hit
AGCGAGGGGGCTAAAAGAGAAATGGTCATGTATTCAATCA60.22091310751104565No Hit
AGCGAGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG60.22091310751104565No Hit
AGCGAGGGGGAGGAAGAAGGAGAGGAATACTAAATTAAAT60.22091310751104565No Hit
AGCGAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA50.1840942562592047No Hit
AGCGAGGGGGAGGAAGAAGGAGAGGAGTACTAAGTCCATT50.1840942562592047No Hit
AGCGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTT50.1840942562592047No Hit
AGCGAGGGGGTGTTCTTTATTAATATCCTAACACTCCTCG50.1840942562592047No Hit
AGCGAGGGGTGTTACTTTATGCTGCTGGCTCTTGTACCTT50.1840942562592047No Hit
AGCGAGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT40.14727540500736377No Hit
AGCGAGGGGATTCCAAATTAACTAGGCCTATCTGGTTTCT40.14727540500736377No Hit
AGCGAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGC40.14727540500736377No Hit
AGCGAGGGGTATGGCCTACCCATTCCAACTTGGTCTACAA40.14727540500736377No Hit
AGCGAGGGGAGGCTTCACCCTAGATGACACATGAGCAAAA40.14727540500736377No Hit
AGCGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.14727540500736377No Hit
AGCGAGGGGGGGCTGGTGAGATGGCTCAGTTGGTAAGAGC40.14727540500736377No Hit
AGCGAGGGGGAGAGTCTGTTCCGCTGTAGAGGTGACCTGA40.14727540500736377No Hit
AGCGAGGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTG40.14727540500736377No Hit
AGCGAGGGGATTTCATTGAAGACATCTTTGCACATGTGAC40.14727540500736377No Hit
AGCGAGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAG40.14727540500736377No Hit
AGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGT30.11045655375552282No Hit
AGCGAGGGGGTCTGACTTGGGCCTCCAGGGTGTGTACAGT30.11045655375552282No Hit
AGCGAGGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT30.11045655375552282No Hit
AGCGAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.11045655375552282No Hit
AGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA30.11045655375552282No Hit
AGCGAGGGGATGCCATCTACCTTCTTCAACCTCACCATAG30.11045655375552282No Hit
AGCGAGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGC30.11045655375552282No Hit
AGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGA30.11045655375552282No Hit
AGCGAGGGGGGCTGGTGAGATGGATCAGTGGGTAAGAGCA30.11045655375552282No Hit
AGCGAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAGGAGA30.11045655375552282No Hit
AGCGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAAAG30.11045655375552282No Hit
AGCGAGGGGGGGCTGGCGAGATGGCTCAGTGGATAAGAGC30.11045655375552282No Hit
AGCGAGGGGGAATGCCTGGTGGCTTCCCAGGTGGAGGAGC30.11045655375552282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG559.094947E-1234.0000045
AGGGGGA559.094947E-1234.0000045
AGGGGGT251.5431906E-434.05
AGGGGCA200.00232683934.05
CGAGGGG2550.034.03
GGGGGAT200.00232683934.06
GAGGGGT301.0097197E-534.04
GAGGGGG1450.034.04
GAGGGGC301.0097197E-534.04
GAGGGGA501.5825208E-1034.04
AGCGAGG2700.034.01
GCGAGGG2600.034.02
GGGGGGG650.001934520115.6923076
Produced by FastQC (version 0.11.5)