FastQCFastQC Report
Fri 17 Jun 2016
SRR1525990_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525990_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29566
Sequences flagged as poor quality0
Sequence length56
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG1770.598660623689373No Hit
TTGGACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT1470.4971927213691402No Hit
TTGGACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC920.31116823378204694No Hit
TTGGACGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT850.287492389907326No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC830.28072786308597714No Hit
TTGGACGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC700.23675843874720964No Hit
TTGGACGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT570.19278901440844212No Hit
TTGGACGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC490.16573090712304675No Hit
TTGGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC460.15558411689102347No Hit
TTGGACGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA450.15220185348034906No Hit
TTGGACGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC390.1319082730163025No Hit
TTGGACGGGAGCCACATTATTGACAGTCTTGCATTTTGTTTTGTCTCGTGATGGTG390.1319082730163025No Hit
TTGGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG370.12514374619495366No Hit
TTGGACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT350.11837921937360482No Hit
TTGGACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC330.11161469255225598No Hit
TTGGACGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC320.10823242914158154No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC300.10146790232023271No Hit
TTGGACGGGGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGA300.10146790232023271No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGGG150.00803002150.49571238
CAATCCG505.456968E-1250.237242
TAGCACC150.00819501750.237242
TTGGGCG204.0219462E-450.23719834
CCACGAT150.00825057350.1516246
TCCGCCC550.050.1516245
GCACCTT150.00825057350.1516244
ATCCGCC550.050.1516244
TGGTTGA451.2551027E-1050.06632614
GACAGTG204.0903644E-450.0663269
CTGGTTG451.2551027E-1050.06632613
ACCTGGT451.2551027E-1050.06632611
TACCTGG451.2551027E-1050.06632610
CCTGGTT451.2551027E-1050.06632612
TGGCCTT150.00830640750.06632614
AAGCAGT355.0044036E-850.06632210
CCCAATC402.5411282E-949.9813249
CCAATCT355.140828E-849.8966150
CGGGGTC507.2759576E-1249.8121876
CGGGCAT252.0839194E-549.8121876
Produced by FastQC (version 0.11.5)