##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525990_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	29566
Sequences flagged as poor quality	0
Sequence length	56
%GC	53
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.09713860515457	39.0	37.0	40.0	33.0	40.0
2	36.818609213285534	39.0	37.0	40.0	31.0	40.0
3	36.064973280119055	38.0	35.0	39.0	29.0	40.0
4	36.1975918284516	38.0	35.0	39.0	30.0	40.0
5	33.81698572684841	38.0	33.0	39.0	17.0	40.0
6	36.39481160792803	39.0	36.0	40.0	30.0	40.0
7	36.42234323209092	38.0	36.0	39.0	31.0	40.0
8	36.35777582358114	38.0	36.0	39.0	31.0	40.0
9	36.40664952986538	38.0	36.0	39.0	31.0	40.0
10	35.603598728268956	38.0	35.0	39.0	27.0	40.0
11	35.478015287830615	38.0	35.0	39.0	28.0	40.0
12	34.35953460055469	38.0	33.0	39.0	23.0	40.0
13	33.82476493269296	38.0	32.0	39.0	21.0	40.0
14	34.40566867347629	38.0	33.0	39.0	23.0	40.0
15	33.35845227626328	38.0	32.0	39.0	18.0	40.0
16	32.91801393492525	37.0	31.0	39.0	19.0	40.0
17	32.01068795237773	36.0	29.0	39.0	16.0	40.0
18	31.29398633565582	36.0	27.0	38.0	15.0	40.0
19	32.4984779814652	36.0	30.0	39.0	18.0	40.0
20	33.09358722857336	37.0	31.0	39.0	22.0	40.0
21	33.233071771629575	38.0	31.0	39.0	20.0	40.0
22	32.5150172495434	37.0	30.0	39.0	17.0	40.0
23	33.11706013664344	38.0	31.0	39.0	19.0	40.0
24	32.51109382398701	37.0	30.0	39.0	17.0	40.0
25	32.891091118176284	37.0	31.0	39.0	19.0	40.0
26	33.361158086991814	38.0	33.0	39.0	19.0	40.0
27	33.58310221200027	38.0	33.0	39.0	22.0	40.0
28	33.643813840221874	38.0	33.0	39.0	22.0	40.0
29	33.09710478252046	38.0	32.0	39.0	19.0	40.0
30	32.95068659947237	37.0	31.0	39.0	19.0	40.0
31	33.752824189947916	38.0	33.0	39.0	22.0	40.0
32	33.52841101264966	38.0	33.0	39.0	21.0	40.0
33	33.41003179327606	38.0	33.0	39.0	20.0	40.0
34	33.26753703578434	38.0	33.0	39.0	19.0	40.0
35	33.15642968274369	38.0	33.0	39.0	19.0	40.0
36	32.55300006764527	37.0	31.0	38.0	17.0	40.0
37	32.38743827369276	36.0	31.0	38.0	16.0	40.0
38	31.68575390651424	36.0	30.0	38.0	10.0	40.0
39	30.561016031928567	35.0	28.0	38.0	4.0	40.0
40	28.64256240275993	34.0	23.0	38.0	4.0	39.0
41	26.596495975106542	32.0	17.0	38.0	4.0	39.0
42	27.44919840357167	32.0	21.0	38.0	4.0	39.0
43	27.47236690793479	33.0	21.0	38.0	4.0	39.0
44	26.45170127849557	32.0	17.0	37.0	4.0	39.0
45	27.453493878103227	33.0	22.0	38.0	4.0	39.0
46	25.371372522492052	30.0	16.0	36.0	4.0	39.0
47	24.373740106879524	29.0	10.0	36.0	4.0	39.0
48	25.032165325035514	30.0	15.0	36.0	4.0	39.0
49	23.964215653115065	28.0	9.0	36.0	4.0	39.0
50	24.66566326185483	29.0	11.0	36.0	4.0	39.0
51	24.871778394101334	30.0	4.0	36.0	4.0	39.0
52	24.661401609957384	30.0	4.0	36.0	4.0	39.0
53	24.47131840627748	30.0	4.0	36.0	4.0	39.0
54	24.12883041331259	30.0	4.0	36.0	4.0	39.0
55	23.803186092132854	30.0	4.0	36.0	4.0	39.0
56	23.033078536156395	29.0	4.0	36.0	4.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	2.080342285617988
1	2	2.983640431127178
1	3	3.2210616148452687
1	4	3.0724083505386233
1	5	6.230784192207629
1	6	3.2074826683410436
1	7	2.833913202082286
1	8	3.1027228835356553
1	9	2.899325845450811
1	10	4.01332996006321
1	11	-0.8003808945827018
1	12	-8.388104430536803
1	13	-18.516014854729406
1	14	-6.998248391481152
1	15	0.02285607288819591
1	16	-2.0443450133309575
1	17	-6.333773769368541
1	18	-5.205415111755226
1	19	-0.42427315435972446
1	20	-0.5852270580131673
1	21	4.757275315098955
1	22	5.8336518526990595
1	23	4.829448958980407
1	24	6.239556871817779
1	25	5.118880292293614
1	26	5.686814562254106
1	27	5.515764084444029
1	28	5.714268534232708
1	29	5.658128896498781
1	30	6.376727663841958
1	31	4.631376079323836
1	32	4.443025437947146
1	33	3.6428052013185166
1	34	4.141609813404912
1	35	3.950398193182046
1	36	3.663635416290429
1	37	3.6644824664946647
1	38	4.081721691873863
1	39	4.269855123387629
1	40	-8.28749389781855
1	41	1.7582673134037705
1	42	0.102598366239949
1	43	-9.665857296589513
1	44	-14.272222031485045
1	45	-0.8264800010501787
1	46	-3.191787023596479
1	47	-7.832233206831257
1	48	-2.9263307466963298
1	49	-12.287840889711976
1	50	-2.003632658769778
1	51	6.037885103250645
1	52	4.505507564149266
1	53	4.956351257367604
1	54	4.498286494475213
1	55	4.766988289254609
1	56	8.908764471973424
2	1	2.634458844359891
2	2	3.654315731774723
2	3	4.01523367774999
2	4	4.140863484673687
2	5	6.705344784252027
2	6	4.014143167878508
2	7	3.839001083673409
2	8	3.9759883784477736
2	9	3.627355447670979
2	10	4.654865575234346
2	11	3.4706644338169284
2	12	-1.126309795939207
2	13	1.1865753395351604
2	14	3.1974037824318913
2	15	4.521005934127789
2	16	4.255377465854977
2	17	6.3813048615287755
2	18	4.181726423859942
2	19	5.365273561643974
2	20	4.0253187329211535
2	21	6.618977442758531
2	22	7.094058883226349
2	23	6.968209366010932
2	24	7.872766862567087
2	25	6.382525065651617
2	26	5.4749736741874955
2	27	5.920481938283068
2	28	6.071807849681363
2	29	5.754798646730045
2	30	7.292083815553337
2	31	5.26088579255233
2	32	5.4832659559859955
2	33	5.194609086609908
2	34	5.238279563636176
2	35	4.860666463302302
2	36	5.434218209999958
2	37	6.097877471120011
2	38	6.010953884288295
2	39	6.416941154839982
2	40	5.887344215039914
2	41	9.003410699157698
2	42	8.473088653011459
2	43	6.761987291754611
2	44	-2.58165773916312
2	45	8.240124994324475
2	46	8.178240728484926
2	47	4.059071140994831
2	48	7.638424109917917
2	49	0.8980980557089318
2	50	7.272963085911073
2	51	9.396349488079508
2	52	9.536959923076186
2	53	9.260699083454561
2	54	9.625020999563095
2	55	10.38262196178005
2	56	11.051225156524765
3	1	3.103412383222242
3	2	3.6278285411537965
3	3	4.179801632591499
3	4	3.7795955732538005
3	5	7.249417732580298
3	6	3.8756281874182363
3	7	4.073043636864895
3	8	3.7992623688950147
3	9	3.9534518436761914
3	10	4.777748771065873
3	11	3.734667907546843
3	12	4.385631150652195
3	13	4.485316112440067
3	14	5.0953629661053625
3	15	-0.09471269374889246
3	16	6.389550283918375
3	17	6.948391270560478
3	18	8.43921665399455
3	19	6.174218416181539
3	20	5.498180217940689
3	21	7.236867151833646
3	22	7.868700654828604
3	23	7.228739109823351
3	24	7.490222355402519
3	25	7.326511170731944
3	26	6.424170374144076
3	27	6.578319541409421
3	28	6.273718401135987
3	29	6.630178585939774
3	30	5.8066300595828615
3	31	5.565271376573172
3	32	5.293513459242622
3	33	5.149016381442735
3	34	4.419781951825499
3	35	5.059537501079113
3	36	5.840210394107647
3	37	5.625884418579847
3	38	6.9189000414745685
3	39	7.99922513226074
3	40	9.766632100406827
3	41	12.137149300981406
3	42	10.04625408939878
3	43	11.095899580074196
3	44	11.061850728053553
3	45	10.752934373395249
3	46	11.791354058923485
3	47	14.07947930426014
3	48	12.372249554989562
3	49	12.818636483846145
3	50	12.05759183102614
3	51	11.518364251431656
3	52	11.432748025551216
3	53	11.668862348760683
3	54	11.18950211115667
3	55	11.946668857133936
3	56	11.624824809151775
4	1	2.1238205464875506
4	2	2.994741263689619
4	3	3.7386379330691355
4	4	2.821714548503472
4	5	5.91811074169884
4	6	3.4396750827721263
4	7	2.945384062396812
4	8	3.1249245486605375
4	9	3.0954405540539582
4	10	3.5059294050215826
4	11	3.917472944455227
4	12	4.6396476508693
4	13	4.016362573577716
4	14	3.6654888888148704
4	15	4.308239976680984
4	16	6.191547678620935
4	17	7.040879329613887
4	18	7.5434285515195185
4	19	6.088677816963127
4	20	5.89765915845307
4	21	6.746637017226615
4	22	7.62597377684337
4	23	5.627783834096043
4	24	7.085532820013892
4	25	5.786780384800554
4	26	5.326037503974732
4	27	5.622609597857533
4	28	5.114361041170724
4	29	5.0526709871555795
4	30	6.249530624063976
4	31	5.005566643616163
4	32	4.419436168751954
4	33	5.130779299375867
4	34	5.025513606189371
4	35	3.9032196547916627
4	36	4.923579912127618
4	37	5.417026407290226
4	38	5.819464522586163
4	39	6.161622005903816
4	40	1.866067619295233
4	41	10.769368145966212
4	42	9.068833333862521
4	43	8.038583036435462
4	44	10.248129494879432
4	45	8.835869675175537
4	46	10.476113068910461
4	47	10.931411566526748
4	48	10.38310496098175
4	49	9.557672523794036
4	50	9.294239681655753
4	51	8.843157998717807
4	52	7.983768433714484
4	53	7.920273551539669
4	54	7.922893339988626
4	55	8.020919834120473
4	56	9.029948560780085
5	1	3.036864024748418
5	2	3.7010317354750057
5	3	4.047148571367011
5	4	4.2245822635821
5	5	6.99165375742502
5	6	3.7074826683410436
5	7	4.116521897734458
5	8	4.233157666144351
5	9	4.13845628023342
5	10	4.882895177454515
5	11	4.808314757591212
5	12	5.111895569463201
5	13	6.005724275705376
5	14	5.240882043301454
5	15	6.5228560728881995
5	16	6.238263682321218
5	17	8.383617534979287
5	18	9.185889236070864
5	19	7.2931181499881035
5	20	7.47999033329118
5	21	6.865970967272869
5	22	7.9423475048729735
5	23	7.633796785067361
5	24	7.4786873066003885
5	25	7.097141161858833
5	26	6.534640649210623
5	27	5.863590171400553
5	28	5.931659838580529
5	29	6.462476722585734
5	30	7.268032011668044
5	31	5.696593470628187
5	32	5.203895003164536
5	33	5.512370418709814
5	34	6.945957639491866
5	35	5.6895286279646555
5	36	7.207113677159999
5	37	7.838395509972923
5	38	7.146939083178207
5	39	9.835072514691973
5	40	11.125549580442321
5	41	13.95391948731681
5	42	13.385207061892125
5	43	12.96457748601918
5	44	13.18429970764539
5	45	12.977867825036775
5	46	13.938647759012216
5	47	14.73298418447309
5	48	13.617147514173237
5	49	14.668680849418461
5	50	13.95288908036066
5	51	14.103102494554996
5	52	13.440290172844922
5	53	13.869394735628475
5	54	14.911329972736084
5	55	14.462640463167652
5	56	13.278329689364725
6	1	3.254255329096246
6	2	3.614075213735873
6	3	3.612365962671362
6	4	4.181104002712537
6	5	6.817740713946755
6	6	3.9248739726888644
6	7	2.899130593386637
6	8	3.8853315791878273
6	9	3.8123693237116854
6	10	4.035069090497991
6	11	4.351793018460775
6	12	4.959721656419717
6	13	4.353550362661899
6	14	5.023490738953633
6	15	6.1967691163664576
6	16	6.6513071605820855
6	17	7.970574056718419
6	18	8.620671844766512
6	19	7.553987715205494
6	20	6.501729463725962
6	21	7.039884010751127
6	22	7.63799967878602
6	23	6.459883741589103
6	24	7.7612960022525606
6	25	7.4232281183805675
6	26	5.904205866601927
6	27	4.9070684322701155
6	28	4.866442447276185
6	29	5.918998461716171
6	30	5.3984667942767395
6	31	4.848767383671664
6	32	4.443025437947146
6	33	2.9688921578402514
6	34	4.837261987317952
6	35	3.7330068888342183
6	36	5.42450498150782
6	37	5.29491724910336
6	38	6.1686782136129885
6	39	6.98724642773545
6	40	3.8429408847901456
6	41	10.388702096012466
6	42	9.493902714066039
6	43	9.029794877323532
6	44	9.358212751123649
6	45	7.977867825036775
6	46	9.80821297640352
6	47	11.646027662733964
6	48	10.595408383738455
6	49	11.516506936374984
6	50	11.626802123838917
6	51	10.385711190207168
6	52	11.201159738062312
6	53	11.021568648671952
6	54	10.846112581431733
6	55	11.701770897950261
6	56	12.995720993712553
7	1	2.279376102043109
7	2	1.8831573393397392
7	3	2.6804819047003434
7	4	3.045355210442004
7	5	5.868465351627918
7	6	2.8538594799352452
7	7	2.4285991924204495
7	8	2.835090033294108
7	9	2.9389393720208616
7	10	3.1655038731066867
7	11	3.2054162068665732
7	12	4.87179895110571
7	13	4.905724275705374
7	14	4.041848226876333
7	15	5.324788440037953
7	16	6.3537226195192815
7	17	6.8526996605831485
7	18	7.390237062157816
7	19	5.9955336089252995
7	20	5.5326473381221
7	21	5.479497537321176
7	22	6.36167117637056
7	23	6.2622992005263
7	24	6.62264865925739
7	25	5.532406862341926
7	26	4.352515045345889
7	27	4.470836548212141
7	28	4.140838582541882
7	29	4.7571627129239005
7	30	5.220689016498962
7	31	4.173888156618524
7	32	3.578774230217679
7	33	2.8886989211252754
7	34	3.7073102964967006
7	35	4.044117999945328
7	36	5.747693387304924
7	37	5.878492128330411
7	38	6.066745846463231
7	39	6.094009712759608
7	40	5.329897406529273
7	41	9.835561999394109
7	42	9.404530733389706
7	43	8.4428383555844
7	44	10.56207748542317
7	45	9.43728811489185
7	46	11.288406213118495
7	47	11.947960029883717
7	48	10.683814180839907
7	49	11.362400656181745
7	50	10.507478452341335
7	51	10.10793341242939
7	52	10.117101767047817
7	53	10.305143527899006
7	54	10.699252678050094
7	55	9.79065978683915
7	56	9.7377499792198
8	1	3.2325161986614646
8	2	3.7879882572141312
8	3	4.525409440932229
8	4	4.2245822635821
8	5	7.317740713946755
8	6	4.337917450949739
8	7	4.073043636864895
8	8	4.211418535709569
8	9	3.8123693237116854
8	10	5.404634307889296
8	11	5.003966931504259
8	12	5.633634699897982
8	13	5.397028623531462
8	14	5.980012478084063
8	15	6.0228560728881995
8	16	7.694785421451648
8	17	8.101008839327115
8	18	8.859802279549122
8	19	7.5757268456402755
8	20	7.110425115899876
8	21	8.170318793359822
8	22	8.83365185269906
8	23	8.41640548071954
8	24	8.283035132687342
8	25	7.9232281183805675
8	26	7.034640649210623
8	27	5.950546693139678
8	28	6.649051142928357
8	29	6.440737592150953
8	30	6.050640707320216
8	31	6.0009412967151405
8	32	4.682155872729755
8	33	6.338457375231556
8	34	4.663348943839694
8	35	6.167789497529867
8	36	6.402765851073038
8	37	6.29491724910336
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115	26	5.1945785374093845
115	27	5.466074643450241
115	28	4.268616360319662
115	29	5.844464300225489
115	30	5.255609651419594
115	31	3.5754754582058226
115	32	5.012901214344662
115	33	4.827587810014165
115	34	5.1136595028459055
115	35	5.090149745977072
115	36	5.595312434923972
115	37	6.855476255314539
115	38	6.505634735352121
115	39	6.182898601648496
115	40	9.89418312081499
115	41	9.958577872409982
115	42	9.372784701643674
115	43	7.157124069870115
115	44	-0.6014145780688942
115	45	6.696811924415659
115	46	4.3066601813724645
115	47	-7.655214573290882
115	48	2.831433228458952
115	49	-2.12013902635794
115	50	4.246367341230222
115	51	8.064282618778595
115	52	7.590911290857342
115	53	7.224984797740277
115	54	6.77623680503422
115	55	7.844628040807404
115	56	8.247273788743609
116	1	3.2708793700169636
116	2	3.534791326267843
116	3	4.2530309243081845
116	4	3.760387890180567
116	5	7.299837900647525
116	6	3.973467323072498
116	7	3.6024554015707793
116	8	3.852083497346392
116	9	3.7049524439162838
116	10	4.341974461341984
116	11	3.7328671872587336
116	12	3.563302219079567
116	13	4.829253687470079
116	14	4.785639076549536
116	15	-1.8582180959097556
116	16	4.719082096643469
116	17	6.392568941628902
116	18	5.519648826863701
116	19	6.0961872036965445
116	20	6.022189821782231
116	21	5.698451785687187
116	22	6.602194052187553
116	23	6.880599854121073
116	24	6.823955848799873
116	25	6.774890522472642
116	26	4.877351646653082
116	27	5.243385567819985
116	28	5.136263419143191
116	29	6.083960098544814
116	30	6.6631726766296815
116	31	5.2183326010629685
116	32	4.716682726949703
116	33	5.106999574720049
116	34	3.743911603686243
116	35	6.233006888834218
116	36	7.439850250050025
116	37	7.328165330944795
116	38	6.637987676528592
116	39	9.624075072236728
116	40	9.7240150535881
116	41	10.250594679132668
116	42	6.780347726853762
116	43	3.4428383555843993
116	44	3.4490055899983325
116	45	7.245131252146752
116	46	1.6995173242296069
116	47	-5.676222976652227
116	48	0.7642063377026496
116	49	0.9218777803647491
116	50	3.4081320471125736
116	51	6.0915935431483454
116	52	5.572003727832133
116	53	7.054816730513384
116	54	4.395984704193882
116	55	5.2017708979502615
116	56	7.25147546941588
117	1	1.5468974695644704
117	2	1.8515334077158059
117	3	2.200994725213164
117	4	2.635953501040298
117	5	4.727439710602276
117	6	2.0436030696788308
117	7	2.419197483018742
117	8	2.7615857597898312
117	9	2.4244094574909454
117	10	2.203965411568227
117	11	-0.5997119982616326
117	12	-10.814525835219076
117	13	-13.455814185833084
117	14	-2.6102885252604153
117	15	-8.064100448850933
117	16	-7.649696183899522
117	17	-0.032770424886933824
117	18	-4.3559167839960296
117	19	-0.23865442526273384
117	20	-0.9280364225616644
117	21	2.334198392022035
117	22	3.3804745951739754
117	23	1.1588803971074952
117	24	3.00042643703517
117	25	2.6640307939658534
117	26	3.3072159000467494
117	27	4.191349368724964
117	28	3.172462514165815
117	29	3.97083792659911
117	30	4.47538987119982
117	31	2.2183326010629685
117	32	1.1804836319270784
117	33	1.7698955023218588
117	34	1.9158573050437084
117	35	0.8483915042188315
117	36	3.6887190283305635
117	37	2.6109707608090496
117	38	4.178711658429041
117	39	5.067513986263883
117	40	6.737589714221581
117	41	7.626160289992399
117	42	5.01564184450082
117	43	-6.403315490569447
117	44	-0.9310849077392263
117	45	4.150108627712363
117	46	0.237978862691147
117	47	-0.5947750128513221
117	48	0.8863782834040066
117	49	-2.70255660877552
117	50	1.9194442643071454
117	51	6.1934034978994745
117	52	5.714537664483714
117	53	5.6453144680699445
117	54	6.185577464374877
117	55	4.624847821027185
117	56	5.25826279973262
118	1	1.6238205464875506
118	2	2.187197743380139
118	3	3.138057662276104
118	4	2.2443451094319045
118	5	5.8987683819309495
118	6	2.1659806920564577
118	7	2.0730436368648952
118	8	1.4189284171324914
118	9	2.253080786162272
118	10	2.483685691288507
118	11	-1.7430686416182724
118	12	-13.26557478626803
118	13	-9.448821178840078
118	14	-11.393505308477199
118	15	-11.518645903396386
118	16	-9.775570309773645
118	17	0.7749218828053728
118	18	-4.1461265742058195
118	19	-0.9973956840039939
118	20	0.3376978431726023
118	21	4.757275315098955
118	22	4.936418651118036
118	23	5.2148244530515555
118	24	5.68224461885335
118	25	5.055639185574247
118	26	5.412111004941849
118	27	4.310230487606084
118	28	3.5640709057742086
118	29	5.3379707937319765
118	30	5.034830430640373
118	31	2.8546962374266016
118	32	4.970693422136865
118	33	4.986678719105072
118	34	4.230542619729022
118	35	5.551188707016038
118	36	6.790117629729163
118	37	6.624956774795063
118	38	7.119271098988488
118	39	9.31926223801213
118	40	10.111715588347455
118	41	7.643642807474915
118	42	9.197460026319
118	43	1.8973838101298526
118	44	0.5479360712817538
118	45	7.44031841792215
118	46	1.1540627787750601
118	47	-2.213656131732442
118	48	1.88987478690051
118	49	-1.1850740912930036
118	50	5.1781855230484055
118	51	7.794802099298078
118	52	6.711041160987211
118	53	6.260699083454561
118	54	7.091171869969283
118	55	6.883589079768445
118	56	11.7407802822501
119	1	1.94524911791612
119	2	2.505379561561959
119	3	3.36857714279558
119	4	3.3612282263150206
119	5	6.570846304008867
119	6	3.013383289459057
119	7	3.2873293511506105
119	8	3.1819154301195027
119	9	3.2595742926557847
119	10	4.058361015963833
119	11	0.5101781116284769
119	12	-9.690899461592704
119	13	-2.9942757242946243
119	14	-4.981167646139536
119	15	-7.135529020279506
119	16	-7.778817063020401
119	17	0.5606361685196575
119	18	-6.759762937842183
119	19	-0.06557750218581049
119	20	0.324710830185591
119	21	5.900132457956101
119	22	7.001353716053099
119	23	6.065473803700904
119	24	6.071855008463739
119	25	6.153041782976846
119	26	6.087435680266523
119	27	5.394646072021665
119	28	6.090044931748231
119	29	5.630178585939774
119	30	6.862752508562455
119	31	5.146904029634399
119	32	5.298615500058947
119	33	5.756159238585596
119	34	5.542230931417329
119	35	4.983006888834218
119	36	5.702455292066826
119	37	5.998333398171685
119	38	5.148491878209267
119	39	7.325755744505642
119	40	9.537040263672129
119	41	7.494292158124267
119	42	8.908498987357966
119	43	0.12140978415582637
119	44	0.2200139933596752
119	45	8.16109763870137
119	46	0.7709458956581798
119	47	-1.7980717161480264
119	48	-2.918566771541048
119	49	-4.37013902635794
119	50	3.6035101983730797
119	51	9.242854047350026
119	52	9.555197005143057
119	53	8.724984797740277
119	54	9.419093947891362
119	55	10.023199469378834
119	56	11.104416645886467
120	1	1.158303305108241
120	2	1.7122761132860944
120	3	2.409217536884249
120	4	2.082903103162316
120	5	3.6040975355359635
120	6	2.0860433879812206
120	7	1.6592505334166177
120	8	1.870339075439702
120	9	2.047751632557258
120	10	3.5103314593135835
120	11	-1.5784918391104341
120	12	-2.9803083285877783
120	13	-7.2873791725704855
120	14	-8.8370789761888
120	15	-1.2537556212647267
120	16	-7.336698836419416
120	17	0.65423222763296
120	18	-7.285625006807699
120	19	-0.2810947435651201
120	20	-1.3033679875484019
120	21	1.7400339357886132
120	22	4.21071332196443
120	23	2.48911912389795
120	24	3.828012643931725
120	25	3.0963915366714225
120	26	4.178568685192637
120	27	4.330606663154676
120	28	4.104823256871384
120	29	4.063676122885582
120	30	4.639846104621565
120	31	3.6666084631319364
120	32	3.092950475428406
120	33	1.6465533272555462
120	34	2.0988811777227525
120	35	0.7674896474549087
120	36	3.2948198240865345
120	37	3.3099097528514854
120	38	2.8590830112141923
120	39	4.355312394751941
120	40	5.067828441012033
120	41	-3.3751659699545513
120	42	-2.760220224464696
120	43	-3.143368540967323
120	44	-14.884665809595989
120	45	-3.247769356372519
120	46	-5.731517158529012
120	47	-4.871963341763788
120	48	-3.3138869685853827
120	49	-14.479744937687988
120	50	-1.8312188656663295
120	51	3.265021535034755
120	52	2.2596305026800003
120	53	3.950354255868355
120	54	0.5237737508470275
120	55	1.2190122772606067
120	56	6.190623542438189
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	479.0
5	51.0
6	88.0
7	93.0
8	120.0
9	132.0
10	132.0
11	79.0
12	85.0
13	75.0
14	65.0
15	108.0
16	114.0
17	143.0
18	206.0
19	241.0
20	359.0
21	406.0
22	536.0
23	694.0
24	772.0
25	861.0
26	956.0
27	983.0
28	1049.0
29	1109.0
30	1231.0
31	1406.0
32	1601.0
33	1739.0
34	1912.0
35	2100.0
36	2231.0
37	2496.0
38	2537.0
39	2376.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	100.0	0.0
3	100.0	0.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	99.21531488872354	0.7136575796523034	0.04396942433876751	0.027058107285395386
8	99.44192653723873	0.35851992153148887	0.14881959006967463	0.05073395116011636
9	98.88047081106677	0.4329297165663262	0.3889602922275587	0.29763918013934926
10	55.71602516403977	26.523709666508825	8.766826760468104	8.993438408983291
11	37.75620645335859	22.522492051680985	21.626192247852263	18.095109247108166
12	31.184468646418186	18.03084624230535	22.576608266251778	28.208076845024692
13	25.21139146316715	15.152540079821417	26.13474937428127	33.50131908273016
14	23.547317865115335	24.565379151728337	26.970168436717852	24.917134546438476
15	28.296115019883754	20.70629822235818	24.785017504503585	26.21256925325448
16	31.376264762613786	24.679367872491625	23.156576765591687	20.787790599302898
17	29.54806846627427	20.546647723428727	28.51633854272377	21.388945267573238
18	25.617263072448083	21.047825204626935	25.816816613677872	27.51809510924711
19	27.37176884558127	22.73311679523616	23.372580863445663	26.522533495736905
20	28.06708453328816	23.100118583770964	27.27426732170083	21.558529561240046
21	26.811202056416157	20.750186024487586	27.463978894676316	24.974633024419944
22	24.423324088480012	20.367990259081378	29.71318406277481	25.495501589663803
23	22.958804031657987	23.111005885138333	28.160725157275245	25.769464925928432
24	22.4293059125964	22.899472331213637	26.47814910025707	28.193072655932895
25	25.137831895822764	25.729748012853037	25.932690681549133	23.19972940977507
26	29.05768905430553	24.750465234308916	22.2568093385214	23.93503637286415
27	22.124822310972718	25.11338252216882	23.32295403777161	29.438841129086846
28	25.475208009199758	24.49773388351485	23.39173374822431	26.635324359061087
29	28.065718157181575	24.26151761517615	22.2289972899729	25.443766937669377
30	28.092853275582026	21.876691932864105	27.07769355711965	22.95276123443422
31	29.984099597415337	24.53398288169424	23.275482932440205	22.206434588450218
32	30.681280021649414	24.446925106555714	23.222380082538393	21.649414789256475
33	28.82727334281265	24.33136976098585	23.677974135012526	23.163382761188977
34	25.220279246306088	24.935610681848992	29.225972617595232	20.618137454249695
35	24.67488485505283	24.806962882687618	29.602411270658358	20.915740991601194
36	26.67501353546291	26.055766107200867	25.365457498646453	21.903762858689767
37	27.016593294954284	24.361666102268877	27.365391127666776	21.256349475110056
38	28.784951703101168	26.056600576173526	23.951872563972206	21.206575156753093
39	27.816901408450708	24.12987540628386	23.0396803900325	25.013542795232937
40	22.027888582046298	19.815212573727457	24.537179093791554	33.6197197504347
41	19.645848289064368	17.006802721088434	22.29514921546508	41.05219977438212
42	24.009497964721845	25.128900949796474	24.9966078697422	25.864993215739485
43	21.48286392941975	22.466915507295553	26.7967424499491	29.253478113335596
44	23.672318303829297	23.25439162787537	25.191125004247223	27.882165064048113
45	25.47623889905769	23.54755609789167	26.781235170496913	24.194969832553724
46	22.99860861302474	25.81531883123494	25.156955238062917	26.029117317677404
47	19.196383043818198	22.837134989971787	25.55325152122922	32.4132304449808
48	25.267361581156084	23.263841884391496	26.74631108704481	24.722485447407607
49	21.91836596206698	23.295897940487905	26.02381841007023	28.761917687374883
50	23.98332655551037	24.332384438118478	27.43662735529348	24.247661651077674
51	22.653545978961656	25.39192399049881	28.299966067186972	23.65456396335256
52	23.375567450369267	25.82153262416153	27.945660275086386	22.85723965038282
53	23.5697086851833	24.43110523281446	27.90382202326449	24.095364058737747
54	23.458381385252018	23.658503493657147	28.471609795807613	24.411505325283223
55	22.199226436859604	23.753138359231865	28.54380131641447	25.503833887494064
56	23.049801674746583	22.89724378750381	29.958978879208054	24.09397565854155
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	1.0
12	16.5
13	33.0
14	34.0
15	42.0
16	50.0
17	49.0
18	48.0
19	56.0
20	64.0
21	61.0
22	61.5
23	65.0
24	68.5
25	72.0
26	92.5
27	113.0
28	130.5
29	177.5
30	207.0
31	238.0
32	269.0
33	292.5
34	316.0
35	376.0
36	436.0
37	452.5
38	532.5
39	596.0
40	683.5
41	771.0
42	854.5
43	938.0
44	1106.5
45	1275.0
46	1589.0
47	1865.0
48	1827.0
49	1734.0
50	1641.0
51	1650.0
52	1659.0
53	1700.5
54	1843.5
55	1945.0
56	1887.0
57	1829.0
58	1922.5
59	2016.0
60	2066.0
61	2116.0
62	1954.5
63	1613.0
64	1433.0
65	1305.0
66	1177.0
67	952.5
68	728.0
69	663.0
70	598.0
71	508.5
72	356.0
73	293.0
74	229.0
75	165.0
76	123.0
77	81.0
78	77.5
79	93.5
80	113.0
81	77.0
82	41.0
83	24.5
84	8.0
85	5.0
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.5
95	1.0
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.49042819454779135
16	0.05073395116011636
17	0.013529053642697693
18	0.0
19	0.03382263410674423
20	0.1724954339443956
21	0.0
22	0.0
23	0.0
24	0.0067645268213488464
25	0.0033822634106744232
26	0.03720489751741866
27	0.06764526821348846
28	0.0
29	0.15558411689102347
30	0.04735168774944192
31	0.02367584387472096
32	0.013529053642697693
33	0.09470337549888384
34	0.19617127781911656
35	0.1285260096056281
36	0.04735168774944192
37	0.12176148278427924
38	0.2063180680511398
39	0.10146790232023271
40	0.7948319015084894
41	1.0586484475410944
42	0.2908746533180004
43	0.32469728742474463
44	0.45660556044104716
45	0.2164648582831631
46	0.3348440776567679
47	0.5039572481904891
48	0.06088074139213962
49	0.3145504971927214
50	0.19617127781911656
51	0.32469728742474463
52	0.16234864371237231
53	0.26719880944327945
54	0.28411012649665157
55	0.31116823378204694
56	0.2333761753365352
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	29566.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.83717783941013
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.46329743875756	70.53033890279376
2	6.735595692650907	10.62030710951769
3	1.7761379724569908	4.200771156057634
4	0.6692693809258227	2.11053236826084
5	0.3989875155519327	1.5727524859636066
6	0.21021922862413658	0.9943854427382804
7	0.15873696855291947	0.8760062233646757
8	0.09867433180316615	0.6223364675640939
9	0.0686430134282895	0.48704593113711697
>10	0.39040713887339656	5.580734627612798
>50	0.02145094169634047	1.3089359399310017
>100	0.008580376678536188	1.095853345058513
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTGGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	177	0.598660623689373	No Hit
TTGGACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	147	0.4971927213691402	No Hit
TTGGACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	92	0.31116823378204694	No Hit
TTGGACGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT	85	0.287492389907326	No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	83	0.28072786308597714	No Hit
TTGGACGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC	70	0.23675843874720964	No Hit
TTGGACGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT	57	0.19278901440844212	No Hit
TTGGACGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC	49	0.16573090712304675	No Hit
TTGGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	46	0.15558411689102347	No Hit
TTGGACGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA	45	0.15220185348034906	No Hit
TTGGACGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC	39	0.1319082730163025	No Hit
TTGGACGGGAGCCACATTATTGACAGTCTTGCATTTTGTTTTGTCTCGTGATGGTG	39	0.1319082730163025	No Hit
TTGGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG	37	0.12514374619495366	No Hit
TTGGACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT	35	0.11837921937360482	No Hit
TTGGACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	33	0.11161469255225598	No Hit
TTGGACGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC	32	0.10823242914158154	No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC	30	0.10146790232023271	No Hit
TTGGACGGGGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGA	30	0.10146790232023271	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0067645268213488464	0.0	0.0	0.0	0.0
11	0.013529053642697693	0.0	0.0	0.0	0.0
12	0.016911317053372118	0.0	0.0	0.0	0.0
13	0.016911317053372118	0.0	0.0	0.0	0.0
14	0.0439694243387675	0.0	0.0	0.0	0.0
15	0.0439694243387675	0.0	0.0	0.0	0.0
16	0.05073395116011635	0.0	0.0	0.0	0.0
17	0.06088074139213962	0.0	0.0	0.0	0.0
18	0.06088074139213962	0.0	0.0	0.0	0.0
19	0.06426300480281405	0.0	0.0	0.0	0.0
20	0.06764526821348847	0.0	0.0	0.0	0.0
21	0.06764526821348847	0.0	0.0	0.0	0.0
22	0.07102753162416289	0.0	0.0	0.0	0.0
23	0.07102753162416289	0.0	0.0	0.0	0.0
24	0.07779205844551174	0.0	0.0	0.0	0.0
25	0.08455658526686058	0.0	0.0	0.0	0.0
26	0.087938848677535	0.0	0.0	0.0	0.0
27	0.09470337549888386	0.0	0.0	0.0	0.0
28	0.10485016573090712	0.0	0.0	0.0	0.0
29	0.11499695596293039	0.0	0.0	0.0	0.0
30	0.11499695596293039	0.0	0.0	0.0	0.0
31	0.11837921937360482	0.0	0.0	0.0	0.0
32	0.11837921937360482	0.0	0.0	0.0	0.0
33	0.11837921937360482	0.0	0.0	0.0	0.0
34	0.12176148278427924	0.0	0.0	0.0	0.0
35	0.12514374619495366	0.0	0.0	0.0	0.0
36	0.12514374619495366	0.0	0.0	0.0	0.0
37	0.12514374619495366	0.0	0.0	0.0	0.0
38	0.13529053642697694	0.0	0.0	0.0	0.0
39	0.13867279983765135	0.0	0.0	0.0	0.0
40	0.13867279983765135	0.0	0.0	0.0	0.0
41	0.13867279983765135	0.0	0.0	0.0	0.0
42	0.14881959006967463	0.0	0.0	0.0	0.0
43	0.14881959006967463	0.0	0.0	0.0	0.0
44	0.14881959006967463	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTCGGG	15	0.008030021	50.495712	38
CAATCCG	50	5.456968E-12	50.2372	42
TAGCACC	15	0.008195017	50.2372	42
TTGGGCG	20	4.0219462E-4	50.237198	34
CCACGAT	15	0.008250573	50.15162	46
TCCGCCC	55	0.0	50.15162	45
GCACCTT	15	0.008250573	50.15162	44
ATCCGCC	55	0.0	50.15162	44
TGGTTGA	45	1.2551027E-10	50.066326	14
GACAGTG	20	4.0903644E-4	50.066326	9
CTGGTTG	45	1.2551027E-10	50.066326	13
ACCTGGT	45	1.2551027E-10	50.066326	11
TACCTGG	45	1.2551027E-10	50.066326	10
CCTGGTT	45	1.2551027E-10	50.066326	12
TGGCCTT	15	0.008306407	50.066326	14
AAGCAGT	35	5.0044036E-8	50.066322	10
CCCAATC	40	2.5411282E-9	49.98132	49
CCAATCT	35	5.140828E-8	49.89661	50
CGGGGTC	50	7.2759576E-12	49.812187	6
CGGGCAT	25	2.0839194E-5	49.812187	6
>>END_MODULE