FastQCFastQC Report
Fri 17 Jun 2016
SRR1525989_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525989_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2942
Sequences flagged as poor quality0
Sequence length40
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACGGGTACGCACAGTTCTGAGTCGGAGACACGCAGG301.0197144799456153No Hit
TTGGACGGGTACGCACAGTTCTGAGTCGGAGACACGCCGG290.9857239972807614No Hit
TTGGACGGGTACGCACAGTTCTGAGTCGGAGACCCGCCGG140.4758667573079538No Hit
TTGGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT120.4078857919782461No Hit
TTGGACGGGTACGCACAGCTCTGAGTCGGAGACACGCCGG70.2379333786539769No Hit
TTGGACGGGTACGCACAGTTCTGAGTCGGCGACACGCCGG60.20394289598912305No Hit
TTGGACGGGTACGCACAGTTCTGAGTCGGCGACACGCAGG50.1699524133242692No Hit
TTGGACGGGGTTTGTGTCTGCTTGTCTGTGTATACCACCT50.1699524133242692No Hit
TTGGACGGGTACCTGGTTGATCCTGCCCGTAGCCTATGCT50.1699524133242692No Hit
TTGGACGGGAAGCAGTGGTACGAGAGTCTTTTTTTTTTTT50.1699524133242692No Hit
TTGGACGGGCAGAGCTTTTTTTTTTTTTTTTTTTTTTTTT40.13596193065941536No Hit
TTGGACGGGTACGCACAGCTCTGAGTCGGAGACACGCAGG40.13596193065941536No Hit
TTGGACGGGTACGCACAGCTCTGAGTCGGCGACACGCCGG40.13596193065941536No Hit
TTGGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG40.13596193065941536No Hit
TTGGACGGGTACGCACAGTTCTGCGTCGGAGACACGCAGG40.13596193065941536No Hit
TTGGACGGGTTCGACTTTTTTTTTTTTTTTTTTTTTTTTT40.13596193065941536No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC40.13596193065941536No Hit
TTGGACGGGTACGCACCGTTCTGAGTCGGAGACACGCAGG40.13596193065941536No Hit
TTGGACGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.13596193065941536No Hit
TTGGACGGGTACCTGGTTGATCCTGCCCGTAGCATATGCT30.10197144799456152No Hit
TTGGACGGGTACCTGGTTGATCCTGCCCGTAGCATCTGCT30.10197144799456152No Hit
TTGGACGGGGTTTGTGTCTGCTTGTCTGTGTATACCCCCT30.10197144799456152No Hit
TTGGACGGGAAGCTGGTATCGATTTTTTTTTTTTTTTTTT30.10197144799456152No Hit
TTGGACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.10197144799456152No Hit
TTGGACGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGCGC30.10197144799456152No Hit
TTGGACGGGTACGCACCGTTCTGAGTCGGAGACACGCCGG30.10197144799456152No Hit
TTGGACGGGTACGCACAGTTCTGAGTCGGCGACCCGCAGG30.10197144799456152No Hit
TTGGACGGGTACGCACCGTTCTGCGTCGGAGACACGCCGG30.10197144799456152No Hit
TTGGACGGGTACGCACAGTTCTGAGTCGGAGACACCCCGG30.10197144799456152No Hit
TTGGACGGGATTTTGTGGTAGTTTGAATTGTATCACTTCT30.10197144799456152No Hit
TTGGACGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCG30.10197144799456152No Hit
TTGGACGGGGTGATGGTGGTGGTGGTAGGGGTACGCTTAG30.10197144799456152No Hit
TTGGACGGGATGAAACACTGTCTCTTCTATAGGATCCTTT30.10197144799456152No Hit
TTGGACGGGTACGCCCAGTTCTGAGTCGGAGACACGCCGG30.10197144799456152No Hit
TTGGACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.10197144799456152No Hit
TTGGACGGGTACCTGGTTGATCCTGCCAGTAGCCTATGCT30.10197144799456152No Hit
TTGGACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT30.10197144799456152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGGT559.094947E-1234.4736864
GACGGGG950.034.4736864
CGGGAAG356.011669E-734.4736866
TTGGACG2800.034.4736861
CGGGGGG309.378771E-634.4736826
GGGTACG309.378771E-634.4736827
GACGGGC200.002195467734.4736824
GACGGGA900.034.4736824
CGGGTAC403.7982318E-834.4736826
ACGGGTA452.3628672E-934.4736825
ACGGGGG452.3628672E-934.4736825
ACGGGGA309.378771E-634.4736825
ACGGGAG200.002195467734.4736825
ACGGGAA501.4370016E-1034.4736825
TGGACGG2650.034.4736822
GGGAAGC309.378771E-634.4736827
GGACGGG2600.034.4736823
ACACGCC200.00239852233.8793132
CTGAGTC301.0637619E-533.8793121
ACGCCGG200.00239852233.8793134
Produced by FastQC (version 0.11.5)